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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Cell or tissue samples were homogenized and proteins extracted using RIPA (Thermofisher, USA). After quantification ...
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bioRxiv - Biochemistry 2023Quote: ... wild-type and mutant proteins were expressed in Expi293F cells (Thermo Fisher Scientific) using the same protocol.
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bioRxiv - Cell Biology 2023Quote: ... Suspension Expi293 cells used for protein purifications were grown in Expi293 media (ThermoFisher) at 37 °C/8% CO2/80% humidity in an Infors Multitron shaker.
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bioRxiv - Cancer Biology 2023Quote: Proteins were obtained by cell lysis using RIPA buffer (Thermo Fisher Scientific #89901) containing Protease Inhibitor (Thermo Fisher Scientific #78438 ...
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bioRxiv - Biochemistry 2023Quote: Proteins from human cell lines were extracted in IP lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Pierce™ cell surface protein biotinylation and isolation kit (Thermo Fisher Scientific, A44390). LysoSensorTM Yellow/Blue DND160 (Invitrogen™ ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole-cell protein lysates were resolved on 4%–12% Bis-Tris gels (Invitrogen) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: Whole-cell protein extraction was completed using Pierce™ RIPA buffer (ThermoFisher 88901) supplemented with Protease Inhibitor Cocktail (Sigma P8340-5ML ...
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bioRxiv - Molecular Biology 2021Quote: 400000 Schneider SL2 cells were transfected with 50 ng of plasmid bearing the Srrm234 minigene (when it applies) and 3 ug of Srrm protein expression plasmid (or control) using Lipofectamine 2000 (Invitrogen, following manufacturer’s instructions) and plated in 6-well plates ...
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bioRxiv - Genetics 2019Quote: ... SDS-Page separation was completed by running 2 μg of total protein on a NuPAGE™ 3-8% Tris-Acetate gel (Thermo Fisher Scientific). Next ...
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bioRxiv - Biochemistry 2021Quote: Hsc70cb cDNA was amplified using PCR and inserted into the pProEX-HTA protein expression vector using 5’ SacI and 3’ SpeI restriction enzyme sites (Invitrogen, Carlsbad, CA, USA). Two Drosophila NBD constructs (with or without a stop codon ...
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bioRxiv - Plant Biology 2022Quote: ... and proteins were loaded at equal Chl content in the native gel (NativePAGE™ 3 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well from ThermoFisher catalog number BN1001BOX). Prior to loading the samples were supplemented with sodium deoxycholate (final concentration 0.3%) ...
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bioRxiv - Genomics 2021Quote: ... and run on a NuPAGE™ 3-8% Tris-Acetate Protein Gel with NuPAGE™ Tris-Acetate SDS Running Buffer (ThermoFisher Scientific, LA0041) in a XCell SureLock™ Mini-Cell (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: PGT145 and VRC26-family antibodies were captured with Protein A columns (Cytiva, 11003493) and gently eluted with 3 M MgCl2 solution (Thermo Fisher Scientific, 21027). Elutions were then buffer exchanged to HEPES buffer (10 mM HEPES pH 8.0 and 75 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... two independent IPs were carried out using 120 μg of Arabidopsis chromatin mixed with 3 % of Drosophila chromatin quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). DNA samples eluted and purified from the two technical replicates were pooled before library preparation (Illumina TruSeq ChIP ...
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bioRxiv - Cell Biology 2023Quote: ... Samples (10 µg) were then added to each well and protein complexes were separated on 3-12% NativePAGE gels (Thermo Fisher Scientific, BN1003BOX). Proteins were transferred to PVDF membranes and probed with specific antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... free amino acids from leaf or insect samples were extracted with vortex agitation for 30 sec at room temperature in 1 mL acetonitrile 25 % (Fisher Scientific; Hampton, New Hampshire, USA) in hydrochloric acid 0.01 N (1:3 ...
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bioRxiv - Cancer Biology 2021Quote: ... γδ T cells were incubated with 3 µg/mL purified anti-TCRγδ (clone 5A6.E9, Invitrogen) for 1h at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... U251 cells were treated with 4 uM CellEvent Caspase-3/7 green detection reagent (Molecular probes) prior to imaging ...
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bioRxiv - Immunology 2021Quote: ... 0.75 x106 cells/mL were cultured in 3 mL of RPMI1640 medium (Gibco Cat. #23400-021) in a 6-well cell culture plate (Peak Serum Inc ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed 3 times and then left in warm PBS without calcium and magnesium (Thermofisher). Cells were imaged every 60 minutes for 20 hours using a 20X air objective (NA 0.75 ...
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bioRxiv - Immunology 2021Quote: ... cells were primed with 200 ng/ml LPS for 3 h in Opti-MEM® (Gibco) or DMEM supplemented with 10% FBS before NLRP3 stimulus was used ...
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bioRxiv - Bioengineering 2019Quote: ... n=3 pellets/condition/cell-type) and cultured in differentiation media (StemPro Chondrogenic Media; ThermoFisher Scientific) for 28 days ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells of both the PC-3 and GS689.Li lines were cultured in DMEM/F12 (Gibco) supplemented with 10% FBS (Atlanta Biologicals ...
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bioRxiv - Bioengineering 2019Quote: Cells were loaded with the Ca2+sensitive fluorescence indicator Fluo-3/AM (5 µM, Life Technologies) together with 0.1% Pluronic f-127 (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... The cells were passaged every 2-3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer.
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bioRxiv - Immunology 2021Quote: ... Target cell killing was measured using CellEvent™ Caspase-3/7 Green Detection Reagent (Life Technologies) and analyzed by flow cytometry.
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bioRxiv - Biochemistry 2020Quote: ... non-denatured cell lysates were separated on NativePAGE™ 3-12% Bis-Tris gel (Thermo Fisher) following manufacturer’s procedure ...
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bioRxiv - Cancer Biology 2020Quote: ... cell viability (n =3) was assayed at different times using the PrestoBlue cytotoxicity assay (Thermo Fisher), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Poly (I:C) and 2’-3’ cGAMP were delivered into cells using Lipofectamine 2000 (Thermo Fisher, USA) as described previously.
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bioRxiv - Bioengineering 2022Quote: 3×105 Luc-1B3 cells were seeded and grown on Thermanox cover slips (150067, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were allowed to adhere for 1-3 hours before media was changed to Neurobasal (Invitrogen) supplemented with 1x B27 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... cells were washed 3 times with PBS before addition of LPS diluted in Opti-MEM (Gibco). Cell lysates and supernatants were collected 24 hours post treatment (hpt) ...
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bioRxiv - Biophysics 2021Quote: ... Cells at 80% confluence were transiently transfected with 3 μg DNA using Neon electroporation system (Invitrogen). EGFR-mEOS and Actin-mapple were used for Neon transfection.
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bioRxiv - Immunology 2020Quote: RNA from 1-3 x 106 cells was extracted and purified with TRIzol reagent (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were transfected with ASO 3 or NTC ASO using lipofectamine 2000 (Thermo Fisher Scientific) or TransIT-X2 (Mirus Bio).
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bioRxiv - Cancer Biology 2022Quote: ... DM-3 and RS-5 cells were cultured in NCTC-109 medium (Gibco, Carlsbad, CA, USA) supplemented with antibiotics ...
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bioRxiv - Cancer Biology 2022Quote: 500000 cells in suspension were loaded with 3 μM Fluo-4 AM (Thermo Fisher Scientific, F14201) in DMEM without serum supplemented with 0.02% Pluronic-F127 (Sigma ...
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bioRxiv - Genetics 2019Quote: ... Twenty micrograms of whole-cell extracts were loaded on 3-12% gradient native-PAGE gels (Invitrogen) and subsequently subjected to a 16-h electrophoresis at 45 V using a running buffer consisting of 50 mM BisTris/Tricine ...
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bioRxiv - Molecular Biology 2019Quote: Whole cell lysates were resolved in NuPAGETM 3-8% or 4-12% gradient gels (ThermoFisher Scientific) and transferred onto PVDF membranes using the Trans-blot Turbo transfer system (BioRad) ...
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bioRxiv - Neuroscience 2019Quote: Dead and apoptotic cells were detected using CellEvent Caspase-3/7 Kit (#C10423, Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2020Quote: ... After incubation cells were washed with Aldefluor® buffer containing TOTO™-3 Iodide (Invitrogen™) to counterstain and exclude dead cells in subsequent flow cytometric analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... PC-3 cells were cultured in 10% FBS in F-12K Kaighn’s modification + L-Glutamine (Gibco). Cell culture experiments were performed in FBS-containing media.
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bioRxiv - Cell Biology 2020Quote: Mouse bone marrow cells were isolated by crushing entire long bones into DMEM/3% FCS (GIBCO). Bone fragments were removed by filtration through a 70 μm filter mesh ...
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bioRxiv - Molecular Biology 2021Quote: ... 4.105 cells were transfected with 3 μg of each gRNA plasmid by Lipofectamine 3000 (Thermo Fisher). Three days post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed 3 x 30 minutes with 100µl of phosphate buffered saline (PBS; #P5119; Gibco) at 37°C to remove excessive mucus ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 2B4 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO, Grand Island, NY) supplemented with 20% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and MCR:SATB2 (45-3 and 63-4) cell lines were cultured in DMEM medium (Life Technologies) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Prostate cancer cell lines: PC-3 and DU145 were cultured in DMEM/F12 (#31330-038, Gibco) with 5% FBS ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with siRNA (5’-GGACTGAGGUUGUCAAGAA-3’) for PRC1 using Oligofectamine (Life Technologies, Carlsbad, CA) as previously described (Udy et al. ...