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Citations for Thermo Fisher :
9251 - 9300 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Whole protein extracts were prepared from JJN3 pellets (4°C, 150 x g, 5 min) according to the cell extraction protocol for ELISA sample preparation by ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... A Lsm12-KO cell line of HEK293 cells was generated with Synthego’s chemically modified sgRNA 5’-CCAGAAUGUCCCUCUUCCAG-3’ and GeneArt Platinium Cas9 nuclease (ThermoFisher Scientific) that were transfected together into cells using Lipofectamine CRISPRMAX Cas9 transfection reagent (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Cell Biology 2020Quote: ... 20*106 THP-1 cells were treated with doxycycline (200 ng/ml) for 5 h and stimulated with L18-MDP (200 ng/ml, Invitrogen) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 × 0.075 μm, 2 μm particle size, Thermo Fisher Scientific), coupled to a Linear Trap Quadrupole Orbitrap Velos (with CID ...
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bioRxiv - Cell Biology 2020Quote: ... RSLC systems were equipped with C18 μ-precolumns (PepMap™, 5 mm x 0.3 mm; Thermo Fisher Scientific, Bremen, Germany) and C18 reversed-phase nano RSLC columns (Acclaim PepMap™ ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed three times with PBS 0.1% Triton X-100 for 5 minutes and nuclei were counterstained with 2 μg/μl Hoechst 33342 (Invitrogen H3570) in PBS 0.1% Triton X-100 for 5 minutes at RT ...
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bioRxiv - Cell Biology 2020Quote: ... sections / cells were then incubated in PBS−/− containing either DAPI (5 μg ml−1; 10 min; RT; Thermo Fisher Scientific) or DRAQ5 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: 1 mg of each of the two pX335 guide RNA/SpCas9n constructs and 5 mg of the Citrine donor template were transfected into 1 million OP9 cells using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... cells were rinsed 4x for 5 minutes in PBS and mounted on glass slides using ProLong Gold antifade reagent (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... samples were washed thrice with PBS having 0.3% Triton X-100 (PBTX) and blocked using 5% Fetal Bovine Serum (ThermoFisher Scientific) in PBTX for 2 hrs at RT ...
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bioRxiv - Immunology 2021Quote: OT-I T cells were isolated from spleens of CD45.1 mice and intraveneously injected (1×107 cells) either unlabeled or following CFSE labeling (5 µM, Molecular Probes) into recipient CD45.2 DC ICAM-1 KO mice and their control counterparts ...
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bioRxiv - Immunology 2021Quote: Thymi from 4-week-old wild type mice were harvested and 5 million thymocytes per condition were cultured in IMDM medium (Gibco) with 2% FBS (HyClone Thermo Fischer Scientific ...
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bioRxiv - Immunology 2021Quote: ... monocyte-derived DCs that were transfected with hACE2 were seeded at 0.5×106 cells/mL in a 6-well plate and transfection was performed with Lipofectamine LTX and PLUS reagents (Invitrogen) according to the manufacturer’s instructions for primary cells ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed 3 times with washing buffer (1x PBS and 0.025% of Tween20) for 5 min and incubated for 1h at RT with the Alexa Fluor secondary antibodies (Thermo Scientific). Cells were washed twice with washing buffer for 5 min and incubated in with DAPI (1 μg/mL ...
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bioRxiv - Genetics 2021Quote: The SNP STARRseq library (100ug plasmid DNA/replica) was transfected into LNCaP cells (5 × 107 cells/replica; 3 biological replicas) using the Neon Transfection System (Invitrogen). Cells were grown in RPMI 1640 medium supplemented with 10% FBS and collected 48hrs post-electroporation ...
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bioRxiv - Genetics 2021Quote: ... designing primers to full length MC1R and cloning PCR product of cDNA and genomic from NK cells from 5 heterozygous individuals heterozygous using TA Cloning kit (Thermofisher). In addition ...
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bioRxiv - Genetics 2021Quote: ... cDNA was synthetized from 5 μg of RNA with the Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Scientific) and oligo (dT)18 primer ...
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bioRxiv - Physiology 2020Quote: ... and 5 μl were subjected to high pressure liquid chromatography (HPLC) in a 25 cm PepMap RSLC C18 column (Thermofisher) coupled to tandem mass spectrometry (MS/MS ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 ng cDNA was subjected to real time qPCR using PowerUp™ SYBR® Green Mastermix (A25741, Thermo Fisher Scientific) on the QuantStudio 3 Real-Time PCR system (A28567 ...
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bioRxiv - Immunology 2021Quote: Splenocytes were processed as described in the previous section and stimulated with RBD peptides for 5 hours at 37°C with protein transport inhibitor (Invitrogen) and anti-mouse CD107a-FITC antibody (BioLegend) ...
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bioRxiv - Biochemistry 2020Quote: Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed three times for 5 min in PBT and incubated in Alexa Fluor 488 or 594 secondary antibodies 1:500 (Invitrogen) for 72 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... 1 μg of this plasmid was transfected into 5×105 SH-SY5Y cells with Lipofectamine LTX PLUS (1:1 ratio; Invitrogen) and selected for colonies with G418 (0.5 mg/ml) ...
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bioRxiv - Microbiology 2019Quote: ... The alkaline hydrolysis ladder was generated by incubating the end-labeled transcript at 95 °C for 5 min in alkaline buffer (Ambion). RNase T1 was used for 5 min at 37 °C to generate the G ladder ...
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bioRxiv - Microbiology 2019Quote: ... 0.1 pmol of 5’-end labeled manY and thrS mRNAs were incubated at 37 °C for 30 minutes in structure buffer (Ambion) containing 1 ng of yeast RNA (Ambion) ...
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bioRxiv - Molecular Biology 2021Quote: ... Individual reactions were heated to 65 °C for 5 min and transferred to ice for 3 min to facilitate annealing in SuperScript III reaction buffer (Invitrogen). After annealing ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by mechanical and enzymatic dissociation by pipetting every 5 minutes in protease solution (0.25% trypsin (Life Technologies, 15090-046), 1 mM EDTA ...
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bioRxiv - Developmental Biology 2021Quote: A gene fragment spanning from 488 base pairs upstream from the 5’UTR through 61 base pairs downstream from exon 1 was cloned into pDONR221 using a BP reaction (ThermoFisher) using the primers prkci-BP-forward 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTCctatctaggtatatgggccctc-3’ and prkci-BP-reverse 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTcgcaatcctgagaataagtgaga-3’ ...
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bioRxiv - Developmental Biology 2021Quote: ... Alkaline phosphatase staining was performed using the one-step nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP) solution (Thermofisher).
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bioRxiv - Developmental Biology 2021Quote: ... at 5 m/s for 30 s with 1.4 mm ceramic beads (15-340-153, Fisher Scientific, Waltham, MA, USA). HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Institution Code: CBTCCCAS) and were cultured at 37°C in a humidified incubator with 5% CO2/95% air with MEM (Gibco) supplemented with 1.5 mg/ml NaHCO3 ...
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bioRxiv - Microbiology 2021Quote: ... 11 µl of nucleic acids were incubated for 2 min at 72°C with 100 pmol of primer A (5’GTTTCCCACTGGANNNNNNNN3’) followed by a reverse transcription step using 200 units of Superscript III (Invitrogen) at 50°C for 60 min with a subsequent Klenow DNA polymerase step using 5 units (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: HCT8 cultures infected with 5 × 106 oocysts were for 48h were treated with Trypsin-0.25% EDTA (ThermoFisher Scientific, Waltham, MA), pelleted ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:2000 anti-Ty1 (Invitrogen), then 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Genomics 2021Quote: ... and the amplified fragments were visualized by electrophoresis on agarose gels (2% for DBP1 and 1% for RBP2a) in 1x TAE buffer (40 mM Tris-acetate, 1 mM EDTA) with 5 μg/ mL ethidium bromide (Invitrogen) in a horizontal system (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 kb of the 5’ flanking region of MpBLD10 with a SmaI site was introduced into the pENTR/D-TOPO vector (Invitrogen). The chimeric sequence was introduced into pMpGWB307 (Ishizaki et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... freshly cultured hESC colonies were dissociated into clumps (2-5 cells) with Versene buffer (with EDTA, Gibco, Cat. No. 15040066). The dissociated cells were seeded on Geltrex-coated 6-well tissue culture plates at 25,000 cells/cm2 (0.25M per well in the 6-well plates ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 ng of isolated total RNA was used as a template for the RT performed using SuperScript IV (Invitrogen, 18090050), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... for 5 min at room temperature with gentle agitation followed by mounting samples with ProLong Gold antifade reagent with DAPI (Invitrogen). Images were collected with an Olympus FluoView-1000 confocal microscope and processed using Olympus FluoView Ver.2.0a Viewer software at University of California ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were indexed by ligating TruSeq Illumina adaptors by incubating DNA-bound beads in 50 μl adapter ligation mix (1x T4 Ligation buffer, 5% PEG-4000, 0.3 U/ μl T4 DNA Ligase (ThermoFisher, EL0011), 1.5 μl TruSeq index adapter) ...
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bioRxiv - Developmental Biology 2020Quote: ... and cultured at 37 °C in a humidified 5% CO2 atmosphere with kidney culture medium (F12:DMEM (1:1) + Glutamax (Gibco) supplemented with 10% fetal bovine serum and penicillin-streptomycin)(Ihermann-Hella & Kuure ...
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bioRxiv - Developmental Biology 2020Quote: ... at 5 m/s for 30 s with 1.4 mm ceramic beads (15-340-153, Fisher Scientific, Waltham, MA, USA). HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154 ...
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bioRxiv - Genomics 2020Quote: ... The HEK293T cells were seeded in a 96-well plate at a cell density of 1.5 x 104 and transfected the next day with 100 ng of mini-genes using Lipofectamine 3000 transfection reagent (Invitrogen), in 4 biological replicates ...
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bioRxiv - Genomics 2020Quote: ... the DNA from above was incubated with 1 μl (5 U/μl) of single-strand specific S1 nuclease (ThermoFisher Scientific) in 1x reaction buffer (40 mM sodium acetate ...
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bioRxiv - Bioengineering 2022Quote: ... DAPI was used as a counterstain at 300nM in 1xPBS for 5 minutes at room temperature and then mounted in mounting medium ProLong Diamond Antifade (ThermoFisher). Slides were imaged on a Keyence BZ-X810 microscope using the 10X objective ...
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bioRxiv - Neuroscience 2022Quote: ... and maintained in culture in Ham’s F-12 culture medium (PAA Laboratories) with 5% foetal bovine serum (Thermo Fisher Scientific). Cell culture media containing sNEGR1 secreted by the cells was collected and centrifuged for 15 min at 5000 g at 4°C to remove cell debris ...
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bioRxiv - Immunology 2022Quote: HEK293-hACE2 cells15 or VeroE6-TMPRSS214 were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep and 8ug/mL puromycin for TMPRSS2 maintenance with 5% CO2 in a 37°C incubator (ThermoFisher). One day prior to infection ...
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bioRxiv - Immunology 2022Quote: ... The cells were individually collected using an automated micro-manipulating system (CellCelector, ALS) and deposited in nuclease-free microtubes containing 5 μL of 2× CellsDirect buffer and RNase Inhibitor (Invitrogen). Single cell RT-qPCR was then performed using the protocol ADP41 developed by Fluidigm ...