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Citations for Thermo Fisher :
8901 - 8950 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the tubules were digested using 10 ml of DMEM-F12 buffer containing 5 mg of trypsin (Thermo Fisher 27250018) on a 50 ml conical tube and the reaction was carried out on a 35°C shaker water- bath ...
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bioRxiv - Bioengineering 2023Quote: ... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.
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bioRxiv - Cancer Biology 2023Quote: ... and the mouse macrophage cell line RAW 264.7 were cultured at 37°C in 5%CO2 in RPMI-1640 medium (Invitrogen) containing 10% (vol/vol ...
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bioRxiv - Immunology 2023Quote: NIH/3T3 mouse embryonic fibroblast cells (ATCC) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) (ThermoFisher Scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 2-5 µL of 50 µM siRNA stock was resuspended in 500 µL OptiMEM reduced serum media (ThermoFisher 51985034) and gently inverted to mix ...
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bioRxiv - Synthetic Biology 2023Quote: ... 3 nM of fluorescently labeled and biotinylated DNA (or 2x1.5 nM with experiments for the intermolecular cases) were bound to 5 nM Streptavidin in PBS and with 1:1800 Tetraspeck beads (0.1 µm, ThermoFisher). After ∼10 min incubation time ...
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bioRxiv - Plant Biology 2023Quote: ... 30 ng of the cDNA were used for RT-qPCR analysis (QuantStudio 5 Real-Time PCR system; Applied Biosystems). Ct values were analyzed with QuantStudio design and analysis software (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... or CDLK5 in 10 mM HEPES-NaOH pH 7.4 and 500 mM NaCl was incubated with B1 (12.5, 25, or 50 µM) in the presence of 5× SyPRO orange dye (Invitrogen). Next ...
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bioRxiv - Biophysics 2023Quote: MDCK cells were cultured at 37 °C in an atmosphere of 5% CO2 in DMEM (1X) + Glutamax (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (18,253 xg, 5 min, 4°C) and protein concentrations determined by absorbance on a NanoDrop instrument (ThermoFisher). Equal amounts of lysate were mixed with 4x loading dye (2% SDS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated at 37 °C with 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) (11965; Thermo Fisher Scientific) with 25 mM HEPES (15630080 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with PBS for 5 minutes at RT and F-actin was stained with Phalloidin-AF555 (Invitrogen) in Blocking buffer for 30 minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were used at a ratio of 1:500 in 5% GS/1% BSA/0.2% PBST (anti-rabbit-Alexa-Fluor-555, Invitrogen A27039 ...
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bioRxiv - Bioengineering 2023Quote: ... we incubated the cells for 5 minutes with a permeabilization solution of 0.1% Triton X-100 (ThermoFisher, A16046.AE) in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... The macrophages were cultured in RPMI 1640 Medium (ATCC) growth media supplemented with 5% fetal bovine serum (FBS, ThermoFisher) and 1% Antibiotic -Antimycotic (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... and rho kinase inhibitors such as a CEPT cocktail consisting of 5 µM Emricasan (50-136-5235, Fisher Scientific), 50 nM Chroman 1 (7163 ...
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bioRxiv - Developmental Biology 2023Quote: ... The remaining part of embryo was treated with 5 μL Cells-to-cDNA™ II Cell Lysis Buffer (Invitrogen), and then reverse-transcribed into cDNA with the HiScript II Q Select RT Kit (Vazyme).
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bioRxiv - Synthetic Biology 2024Quote: Kinetic constants of SrIRED for cyclohexanone were determined in 100 mM Tris pH 8.0 with 0.5 mM NADPH and varying concentrations of cyclohexanone (0.06 mM - 35 mM) in transparent Nunc 96-well plates (ThermoFisher) and by monitoring the depletion of NADPH concentration at λ of 340nm (Spectramax 190 ...
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bioRxiv - Biochemistry 2024Quote: ... Reaction mixtures were adjusted to a total volume of 5 μl (2 μl solution A; 1,5 μl solution B; 0,3 μl RiboLock (40 U/μl, Thermo Scientific); 0,2 μl D-Luciferin (10 mM ...
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bioRxiv - Immunology 2024Quote: ... the cells were seeded at 5 × 104 cells per well in U-bottom 96-well plates (Fisher Scientific; #163320). Two dilutions of antibody ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was placed into a hybridization bottle and was prehybridized with 5 ml ULTRAhyb Ultrasensitive Hybridization Buffer (Invitrogen) at 42°C for 45 minutes in a hybridization oven ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of culture supernatant was collected and treated with 2 µl (5 U) DNase solution I (ThermoFisher Scientific) at 37°C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... 5 × 105 cells/ml were plated in three 50 mm non-treated tissue-culture dishes (122-17, Thermo Scientific). For control ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 2-5 µg of the indicated plasmid using Lipofectamine 3000 as per manufacturer’s instructions (ThermoFisher). Transfection medium was exchanged to fresh medium and cells were moved to 10 cm plates 4-6 hours post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Physiology 2024Quote: Platelet intracellular Ca2+ was measured as described previously using 5 µM Fluo-4 fluorescence dye (Invitrogen, Waltham, Massachusetts, USA).20 1 x 105 washed platelets were labeled with Fluo-4 for 30 min at room temperature (RT) ...
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bioRxiv - Physiology 2024Quote: 5 μL hemolymph of each biological replicate was transferred into pre-chilled siliconized glass vials (Fisher Scientific 03-395G) on ice with a siliconized glass pipette and supplemented with 150 μL of chilled PBS from the same tip ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were maintained in a water jacketed 5 % CO2 incubator and passaged every 2-3 days using trypsin/EDTA (Gibco). 1E6 cells were seeded into 6 well plates ...
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bioRxiv - Immunology 2024Quote: 2×106 THP-1 cells were centrifuged for 5 min at 1200 rpm and washed in 1X PBS (Gibco). They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza ...
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bioRxiv - Genetics 2024Quote: ... The paraffin blocks were cut into 5-um-thick sections using a Microm HM 325 microtome (Thermo Scientific, USA) and allowed to dry on glass slides overnight at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... were cultured in a humidified incubator (5 % CO2, 37 °C) on 0.1 % gelatin-coated cell culture dishes in ESLIF medium (GMEM (Gibco) containing 10 % fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were maintained in a humidified incubator at 37°C with 5% CO2 in DMEM (GIBCO-11995) supplemented with 10% heat-inactivated fetal bovine serum (#10082147 ...
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bioRxiv - Genomics 2024Quote: ... Real-time quantitative PCR reactions were performed using SYBR Green reagent and analyzed using QuantStudio 5 System (Applied Biosystems). HPRT and B2M were used as housekeeping genes ...
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bioRxiv - Genomics 2024Quote: 129S1/SvImJ / C57BL/6 mESCs24 were cultured in a humidified incubator (5 % CO2, 37 °C) on 0.1 % gelatin-coated cell culture dishes in ESLIF medium (GMEM (Gibco) containing 10 % fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: Passaging of ROBO1+ sorted Rounded/CBC cultures every 4-5 days is best done by incubating cells with 0.25% trypsin-EDTA (Gibco) for 1-2 min at RT/37°C ...
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bioRxiv - Physiology 2024Quote: ... the peptides were concentrated on an Accalaim μ-Precolumn (0.5 mm × 3 mm, particle size 5 μm; Thermo Scientific) in the isocratic mode at a 10 μL/min flow for 5 min in the mobile phase C (2% acetonitrile ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5-LOX was stained with Alexa Fluor™ 555 goat anti-mouse IgG (1:500, # A21422, Lot: 10143952; Invitrogen), and perilipin-2 was stained with Alexa Fluor™ 488 goat anti-rabbit IgG (1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycopeptides were trapped on a C18 cartridge column (Acclaim PepMap100 C18, 0.3 mm I.D. × 5 mm; Thermo Fisher Scientific) and separated on a C18 tip column (75 µm I.D ...
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bioRxiv - Physiology 2024Quote: FAP proliferation was detected using 5-ethynyl-2’-deoxyuridine (EdU)/click-it assay (cat. no.: C10337 and C10340, Invitrogen). Experiments were carried out in ECM coated 96-well half-area plates ...
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bioRxiv - Microbiology 2024Quote: The samples prepared for light microscopy were sectioned at 5 μm with an HM 325 rotary microtome (ThermoFisher-Scientific) and stained them with hematoxylin and eosin ...
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bioRxiv - Neuroscience 2024Quote: ... once in 0.5% BSA with 0.05% Tween20 in PBS for 10 minutes and incubated with secondary antibodies and Hoechst33342 (H1399, ThermoFisher) for 1 hour at RT in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... GCT cell lines were routinely maintained at 37°C with 5% CO2 in complete medium consisting of Dulbecco’s modified Eagle medium: nutrient mixture F-12 (DMEM/F12) (Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2024Quote: ... human female) cells were cultured in a 37°C 5% CO2 and 20% O2 humidified incubator with DMEM (Gibco) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed three times (in 0.1% Tween-20 in PBS (PBST) before incubation with secondary antibodies (5 μg/ml; Invitrogen) in 5% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR master mix was prepared by mixing 5 µL SYBR Green PCR Master Mix (Thermo Fisher Scientific, Cat# A25780) with 0.5 µL primer mix (forward and reverse 10uM ...
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bioRxiv - Cancer Biology 2024Quote: OSCC cells were collected and suspended in PBS containing 5 μM C11-BODIPY 581/591 (Thermo Fisher Scientific, #D3861) in the dark at 37°C for 30 min and washed 3 times with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Cell Biology 2024Quote: ... AML12 cells were cultured at 37°C in 5% CO2 in DMEM/Ham’s F12 50/50 media (Fisher Scientific) with 1X Insulin-Transferrin-Selenium (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... HEK 293T and MEF cells were cultured at 37°C with 5% CO2 in DMEM/F12 medium (Thermo Fisher Scientific – Life technologies #LTI 11039-047 ...
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bioRxiv - Immunology 2024Quote: ... lymph node suspensions at 107 cells per mL in PBS 0.5%FCS were labelled with 5 µM CTV (Invitrogen) for 20 min at 37°C before reaction was quenched and cells washed with cold medium ...