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Citations for Thermo Fisher :
8851 - 8900 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed with PBS (4 x 5 min) and mounted on microscope slides with ProLong Gold (ThermoFisher Scientific). Slices were kept in the fridge for at least two weeks before confocal imaging ...
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bioRxiv - Neuroscience 2023Quote: ... After addition of protein G-coated magnetic dynabeads (5 μl beads/μg antibody, Thermo Fisher Scientific, cat. no. 13424229), the lysate+antibody+bead mixtures were incubated for 1 hour at 4°C with end-over-end mixing ...
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bioRxiv - Cell Biology 2023Quote: ... Chondrocytes were collected and cultured in an incubator containing 5% CO2 at 37°C with Dulbecco’s DMEM/F12 (HyClone) supplemented with 10% fetal bovine serum (FBS, Gibco) and 100 U/ml penicillin G sodium ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then washed 5 times with wash buffer and 2 times with 5x SSC (Gibco ref 15557-044) + 0,1% Tween 20 ...
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bioRxiv - Developmental Biology 2023Quote: ... Separation of metabolites was achieved in 12 minutes using a Hypercarb column (5 mm, 100X2.1 mm column, Thermo Scientific) and a gradient run of solvent A (7.5 mM ammonium acetate with 0.05% ammonium hydroxide ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.4) and then each well was loaded with a cell-permeant 5 μM Fluo-4AM solution (ThermoFisher Scientific) for 40 minutes ...
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bioRxiv - Biophysics 2023Quote: ... HeLa and MDCK cells were cultured at 5% CO2 and 37°C in Dulbecco’s modified Eagle medium (DMEM, Capricorn) with 1% Penicillin Streptomycin (Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% Fetal Bovine Serum (FBS) low in endotoxins and heat-inactivated (Life Technologies, Inc., Rockville, MD, USA) and incubated at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were cultured as neurospheres at 37 °C with 5% CO2 in NPC medium: DMEM/F12 (Gibco, CAT# 10565018) with GlutaMAX supplement ...
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bioRxiv - Developmental Biology 2023Quote: ... Human immortalised endometrial epithelial cells (Ishikawa cells) were cultured at 37°C (5% CO2) in DMEM:F12 B-Nut mix (50:50, ThermoFisher) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was aspirated and cells were resuspended in 5 mL of NB-B27 Plus medium (Neurobasal Plus (NB, Gibco) supplemented with B27 Plus (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Tissue was washed for 5 min on ice with 1× Hanks’ balanced salt solution (HBSS, Gibco, Carlsbad, CA, USA) and cut into small pieces ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were used at a ratio of 1:500 in 5% GS/1% BSA/0.2% PBST (anti-rabbit-Alexa-Fluor-555, Invitrogen A27039; anti-mouse-Alexa-Fluor-647, Invitrogen A21235 ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... Each peptide extracts were loaded on a 300 µm ID x 5 mm PepMap C18 precolumn (Thermo Scientific, USA) at a flow rate of 10 µL/min ...
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bioRxiv - Neuroscience 2024Quote: ... 0.05% Tween-20) overnight at 4 °C and with secondary antibodies diluted in 5% nonfat milk in TBST (ThermoFisher, A16078 [goat anti- mouse HRP ...
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bioRxiv - Biochemistry 2024Quote: ... LUHMES cells were grown at 37°C in a 5% CO2 atmosphere in DMEM/F-12 (Gibco 11330-032) containing N2 supplement (Gibco 17502048 ...
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bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE gels were loaded with 5-15 μL of lysate per lane (NuPAGE 4-12% Bis-Tris; Invitrogen). Lysates were diluted with additional 1x NuPAGE LDS buffer if necessary ...
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bioRxiv - Genomics 2024Quote: HEK293T cells (ATCC CRL-3216) were grown at 37°C and 5% CO2 in Dulbecco’s modified Eagle’s medium (ThermoFisher Scientific) supplemented with 10% fetal bovine serum (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... All cell lines were maintained at 37 °C in 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Gibco 11965126) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... were propagated in 5% of human O+ erythrocytes and 10 mL of RPMI-1640 medium containing 0.5% Albumax II (Invitrogen), 2 mM L-glutamine ...
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bioRxiv - Molecular Biology 2023Quote: 5 μg of RNA were used for poly(A)+ RNA purification with Dynabeads mRNA DIRECT Micro kit (ThermoFisher Scientific). Poly(A)+ RNA was fragmented at 70°C for 5 min in RNA Fragmentation Reagents (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific) using a 120 minute linear gradient for separation with two FAIMS compensation voltages (-45 ...
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bioRxiv - Immunology 2023Quote: ... All staining and blocking solutions were prepared in FACS buffer (a PBS solution with 5% fetal bovine serum (Gibco) and 0.1% sodium azide) ...
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bioRxiv - Molecular Biology 2023Quote: ... Detached cells were centrifuged at 2000 rpm and 4°C for 5 minutes and washed with 1x phosphate-buffered saline (PBS pH 7.4; Gibco) for use downstream.
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bioRxiv - Molecular Biology 2023Quote: ... 5 µg of purified total RNA was then treated with DNase (TURBO DNA-free kit, Thermo Fisher (MA, USA) according to manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... 5′-labelled Aar RNA (4 nM) was incubated for 1 h at 37°C with 1× structure buffer (Ambion), 1 µg yeast RNA and putative mRNA interaction partners at the indicated concentrations (0 nM ...
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bioRxiv - Microbiology 2023Quote: ... The RNase T1 ladder was prepared by denaturing 0.4 pmol of 5′ labelled Aar with 8 µL of 1× sequencing buffer (Ambion) at 95°C for 1 min ...
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bioRxiv - Biochemistry 2023Quote: ... phenol red free 0.5% trypsin EDTA (15400054), PBS (10010-023), and Dulbecco’s phosphate-buffered saline medium (DPBS, 14040-133) were from Gibco. Geneticin (10131-035 ...
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bioRxiv - Bioengineering 2023Quote: ... Mice were sacrificed and spleens were removed and placed in 5 mL of Hanks Balanced Salt Solution (ThermoFisher, 24020117). Using small razors ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% Fetal Bovine Serum (BioWest, cat# S1620) and 1% Penicillin/Streptomycin (Fisher Scientific, cat#15-140-122) unless otherwise noted ...
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bioRxiv - Cancer Biology 2023Quote: Approximately 1 × 107 cells were resuspended in 0.5 ml cold TNE buffer (25 mMTris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, and 1% Triton X-100; Surfact-Amps, ThermoFisher) with a tablet of protease inhibitors (Roche) ...
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bioRxiv - Biophysics 2023Quote: ... About 5 µl of a 1 µg/µl sample was injected into a QExactive-Orbitrap mass spectrometer (Thermo Scientific) and a data-independent acquisition (DIA ...
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bioRxiv - Biophysics 2023Quote: ... The eluted complexes were centrifuged at 16,000 g for 5 minutes and fractionated on a Superdex 200 SEC column (Invitrogen). Proteins and lipids in the SEC fractions were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... Dispase 5 mg/mL [GIBCO], Y-27632 10.5 μM [Sigma], DNase I 10 μg/mL [Sigma], Advanced DMEM/F12 [GIBCO] ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 5 minutes at 95°C and loaded on 4-12% Bis-Tris NuPage gels (Thermo Fisher Scientific). After electrophoretic separation ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of milk was diluted with 50 μL of Tris-Glycine SDS Sample Buffer (Novex, Thermo Fisher Scientific) and 35 μL of milli-Q water ...
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bioRxiv - Synthetic Biology 2023Quote: WT HeLa (ATCC CCL-2) were cultured at 37 °C with 5% CO2 in flasks with Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 1 mM L-glutamine (Gibco) ...
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bioRxiv - Systems Biology 2023Quote: ... We quantified cDNA abundance using quantitative-polymerase chain reaction (qPCR) using the QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Single cell suspensions from dissociated larvae in 5 mL FACS tubes were stained with 1:1000 DAPI (Thermofisher, #62248) to indicate cell viability and FACS was carried out at the QMRI Flow facility using a BD 5 Laser LSR Fortessa cell analyser ...
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bioRxiv - Cancer Biology 2023Quote: ... in PBS/5% FBS at 37°C for 10 min or 2 μM H2-DCFDA (Thermo Fisher Scientific, C6827) in PBS/5% FBS at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... PDOs were centrifuged at 1,200 rpm for 5 min at 4°C and washed with HBSS (Thermo Fisher Scientific). Counting and viability measurements were done using 0.4% Trypan Blue staining solution (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Tryptic peptides were loaded onto a trap column (Pepmap 100 5μm, 5 × 0.3 mm, ThermoFisher Scientific, San Jose, CA) at a flow rate of 10 μL/min using 0.1% TFA as loading buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were cultured as neurospheres at 37 °C with 5% CO2 in NPC medium: DMEM/F12 (Gibco, CAT# 10565018) with GlutaMAX supplement ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae cells (OD 5-10) were pelleted and lysed in 200 µL 0.9% physiologic salt supplied with 2 µL RNAse cocktail (Invitrogen) and 5mg/mL Zymolyase 100T (MP Biomedicals) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cell line was obtained from ATCC and grown at 37°C and 5% CO2 and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (Gibco) ...
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Glioblastoma cells use an integrin- and CD44-mediated motor-clutch mode of migration in brain tissuebioRxiv - Cancer Biology 2023Quote: ... cells were removed from the flask using a 5 minute incubation with 0.25% trypsin with EDTA in Hanks’ balanced salt solution (25-052-CI; Gibco).
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bioRxiv - Cell Biology 2023Quote: ... and HAP1 cells68 were grown in a humidified incubator at 37°C with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (Corning) ...
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bioRxiv - Cancer Biology 2023Quote: ... HeLa Kyoto and RPE1 cells were cultured at 37°C with 5% CO2 in MEM-α medium (Gibco, 12561072) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... All were cultured at 37 °C and in a 5% CO2 atmosphere in high glucose DMEM (Thermo Fisher, 11965084), supplemented with 100 μg/ml penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Amplification and cloning of the truncated versions of TGM4 (D1-3 and D4-5) was performed by PCR amplification using proofreading Taq polymerase Phusion Hi as per the manufacturer’s instructions (Invitrogen), full-length codon-optimised TGM4 as template ...