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Citations for Thermo Fisher :
8801 - 8850 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 5×107 ES cells engineered with T7-dTAG-TAF1 were fixed with 1% formaldehyde (methanol-free, Thermo Fisher Scientific) for 10 min at 25C under constant gentle rotation ...
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bioRxiv - Neuroscience 2023Quote: ... the interscutularis muscle was exposed and labeled with 5 µg/ml Alexa 647-conjugated alpha-bungarotoxin (ThermoFisher Scientific, USA) for 10 minutes at room temperature to stain acetylcholine receptors as a guide for neuromuscular junctions ...
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bioRxiv - Biochemistry 2023Quote: ... coverslips were stained for 5 min with 1 μg/ml 300 nM 4′,6-diamidino-2-phenylindole (Life Technologies) to visualize nuclei ...
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bioRxiv - Biochemistry 2022Quote: ... After membrane permeabilization with 1% Triton X-100/D’PBS for 5 min cells were incubated for 30 min in the primary antibody aPMP70 (rabbit, 1:500, Invitrogen) in D’PBS supplemented with 1% BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic hg13 larvae (mmachchg13/hg13 Tg(col2a1a:EGFP)) were staged and fixed at 5 DPF with 4% paraformaldehyde (Fisher Scientific). Larvae were dissected for genotyping and unbiased imaging was performed on the remaining head tissue ...
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bioRxiv - Cell Biology 2023Quote: ... or CDLK5 kinase domain at 4 μM in 10 mM HEPES-NaOH pH 7.4 and 500 mM NaCl was incubated with the inhibitors at different concentrations (12.5, 25, or 50 μM) in the presence of 5× SyPRO orange dye (Invitrogen). A Real-Time PCR Mx3005p machine (Stratagene ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were maintained in embryo media at 28.5°C until 5 DPF and fixed in 4% paraformaldehyde (Fisher Scientific) at room temperature for 1 hour and stored in 1x PBS (Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured following 15-minute incubation with 5 nM SYTOX™ Red Dead Cell Stain (#S34859, Invitrogen) on ice as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... SPD-5 and GIP-1 cDNAs was amplified by PCR and cloned into the entry vector pDONR201 (Thermo Fisher). Thereafter ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μg of total protein were loaded on NovexTM 10% Zymogram Plus (Gelatin) Protein Gels (Thermo Fisher Scientific: ZY00102BOX) using 2x non-reducing sample buffer (1M Tris-HCl pH 6,8 (Carl Roth ...
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bioRxiv - Cell Biology 2023Quote: ... 300-1500 ng DNA was incubated with 1-5 μl Lipofectamine 2000 in 150 μl Optimem (Thermo Fisher, 31985047) for 10 minutes at room temperature and then given to cells ...
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bioRxiv - Cancer Biology 2023Quote: ... On day 5 the culture medium was replaced with DMEM/F12 containing 1% N2 (v/v, Gibco, Cat#17502048), 1% GlutaMAX (v/v) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cat#30-2020) at 37 °C incubators supplied with 5% CO2 and passaged with Trypsin/EDTA (Gibco, Cat#15400054). For lentivirus production ...
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bioRxiv - Genomics 2023Quote: Library quality was analyzed on an Agilent Tapestation and quantified by qPCR (KAPA KK4835-Thermo Fisher Sci Quantstudio 5) at The Arizona State University (ASU ...
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bioRxiv - Genomics 2023Quote: ... in condΔEXP168 were maintained in 3% hematocrit in human O+ blood in RPMI 1640 supplemented with 5% Albumax-II and 50µg/ml hypoxanthine (Gibco), 25mM HEPES (Sigma ...
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bioRxiv - Immunology 2023Quote: ... cells were restimulated for 5 hr with PMA/Ionomycin (1x in 100 μL per sample Thermofisher, #00-4970-93) in round-bottom 96-well plates ...
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bioRxiv - Cell Biology 2023Quote: ... X-chromosome probes (10 BACS covering approximately 5% of the chromosome) were labeled with AlexaFluor 594 (Molecular Probes, F32949), and chromosome III probes (3 BACS covering approximately 1% of the chromosome ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... MEFs were cultured at 37°C under 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, 12800082), supplemented with 10% FBS (Capricorn Scientific ...
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bioRxiv - Immunology 2023Quote: ... were cultured in vacuum-gas plasma-treated tissue culture flasks (Falcon) at 37°C and 5% CO2 and maintained in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... Cells were co-cultured at 37°C with 5% CO2 during 21 or 25 days in RPMI-1640 (Invitrogen) supplemented with 10% HyClone FBS (Thermo Scientific) ...
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bioRxiv - Genetics 2023Quote: ... MEFs were grown at 37°C and 5% CO2 in high glucose DMEM GlutaMAX™ (ThermoFisher, cat no. 31966021) supplemented with 10% FBS (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by addition of 2.8 μL quenching buffer (0.7 μL of Triton X-100, 0.7 μL of 2% BSA, and 1.4 μL of 5× SuperScript II buffer [Thermo Fisher]). Then ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by the addition of 2.8 μL quenching buffer (0.7 μL of Triton X-100, 0.7 μL of 2% BSA, and 1.4 μL of 5× SuperScript II buffer [Thermo Fisher]) to reduce the denaturing effect of SDc ...
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bioRxiv - Developmental Biology 2022Quote: ... The denaturing effect of SDc was quenched by addition of 0.8 μL of 1:2500,000 diluted ERCC RNA Spike-In Mix 1 and 2.8 μL of quenching buffer (0.7 μL of Triton X-100, 0.7 μL of 2% BSA, and 1.4 μL of 5× SuperScript II buffer [Thermo Fisher]), followed by incubation at 72°C for 90 sec ...
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bioRxiv - Immunology 2023Quote: ... peptides were first loaded onto a trap cartridge (ThermoFisher PepMap, C18, 5 μm, 100 μm i.d. x 20 mm), then eluted onto a reversed phase Easy-Spray column (ThermoFisher PepMap ...
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bioRxiv - Developmental Biology 2023Quote: WTC-mTagRFPT-LMNB1 human iPSCs were dissociated into single cells with 5 min incubation in Accutase (Life Technologies, #A1110501) at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: The collected tissue was quickly washed in PBS twice and centrifuged for 5 minutes at 0.2 x g prior to dissociation with 500 μL of TrypLE Express (Gibco) at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... 5 μL of each restriction digest reaction was combined with 1 μL 6X TriTrack DNA Loading Dye (Thermo Fisher) and run on a 2% agarose gel pre-stained with ethidium bromide ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... Two rounds of gravity settling were performed before plating the cells on laminin (5 μg/ml in PBS, ThermoFisher) coated plates with addition of DMEM/F12 with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... and the extraction was repeated with 50 % ACN / 5 % Formic Acid (HCOOH) (LC-MS Grade, #85178, Thermo Fisher Scientific). The solution was dried in vacuum centrifugation for 2 hours 30 minutes (maximum vacuum pressure up to 5.1 ...
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bioRxiv - Biophysics 2023Quote: ... immunostained cells were exposed for 5 min to 1 mg/100 ml Hoechst 33342 (Thermo Fisher Scientific, Waltham, Massachusetts) in water.
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bioRxiv - Developmental Biology 2023Quote: ... 1×3–5 min with 1x PBS + 1 ng/mL DAPI and mounted in ProLong Gold antifade reagent (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... 10,000 cells were seeded in a 100 mm dish and selected with 5 µg/ml Blasticidin S HCl (Invitrogen). Colonies formed after 2-3 weeks were analyzed for anti-TET repressor by western blots (data not shown) ...
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bioRxiv - Biochemistry 2023Quote: ... at 37°C and under 5% CO2 atmosphere in a HERA cell VIOS 160i CO2 incubator (Thermo Fisher Scientific). The cells were passaged using 1× TrypLETM Express (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... The 5 framework sequences adjacent to the targeted CDRs were amplified using Phusion High-Fidelity DNA polymerase (Thermo Scientific). Framework fragments were assembled with the neighboring mutagenized CDR fragments by sequential ...
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bioRxiv - Microbiology 2023Quote: RK-13 rabbit kidney cell line obtained from the ATCC (ATCC CCL-37) was grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco), containing 10% FBS (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... incorporated 5-EU was detected by the Click-iT™ RNA Alexa Fluor 594 Imaging Kit (ThermoFisher, cat: C10330). Intracellular antibody labelling was performed after the Click reaction ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently centrifuged for 5 minutes at 200 x g and resuspended in 4% paraformaldehyde (Thermo Scientific Chemicals) for 30 minutes at 22°C ...
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bioRxiv - Immunology 2022Quote: ... cells and cellular debris were removed by centrifugation at 4,000 × g for 5 minutes followed by filtration through a 0.22 μm Nalgene rapid-flow filter (ThermoFisher Scientific). Clarified supernatant was incubated with His60 Ni-Superflow resin (Takara Bio ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were cultured at 37 °C in a humidified atmosphere at 5% CO2 with Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS for 1 hour at room temperature and subsequently blocked with 5% bovine serum albumin (ThermoFisher Scientific 37525) and 10% donkey serum (Lampire 7332100 ...
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bioRxiv - Immunology 2023Quote: ... Confluent TeloRF cells were infected with 1 MOI UCD52 RhCMV in low serum (5%) medium in T75 flasks (ThermoFisher). Mock infection was performed in parallel ...
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: Yamato cell lines were seeded into 6-well plates and incubated with 5 µM BODIPY 581/591 C11 (Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Quantitative real-time PCR was conducted using SYBR Green chemistry on a Quant Studio-5 instrument (Applied Biosystems, USA). The reaction volume of 20 µL consisted of 10 µL of TB Green Premix Ex Taq II (Tli RNase H Plus ...
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bioRxiv - Genetics 2023Quote: ... 5 µg of purified total RNA was then treated with DNase (TURBO DNA-free kit, Thermo Fisher (MA, USA) according to manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 25,000 cells were plated onto 24-well plates that were coated with fibronectin (5 µg/cm2, ThermoFisher). To evaluate the effects of exogenous TGFβ ligand or inhibition of TGFβ signaling ...
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bioRxiv - Developmental Biology 2023Quote: ... and p-Smad1/5/8 and thoroughly analyzed under a fluorescent light microscope (EVOS FL, Life Technologies, Carlsbad, CA). Single-plane laser scanning confocal photomicrographs were taken from every other section using a laser scanning confocal microscope (Zeiss LSM 710 ...
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bioRxiv - Immunology 2023Quote: ... we assembled RNP complexes by thoroughly mixing 2.25 ul of 5 ug/ul TrueCut Cas9 Protein V2 (Thermo Fisher) and 5ul 40uM gRNA duplex and incubating at RT for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... for 2 h at 37°C at 5% CO2 in the presence of 0.5 mg/ml dextran-rhodamine (MW=10,000, Molecular Probes). To test sporozoite hepatocyte traversal in the absence or presence of anti-CSP mAbs ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed with PBS (4 x 5 min) and mounted on microscope slides with ProLong Gold (ThermoFisher Scientific). Slices were kept in the fridge for at least two weeks before confocal imaging ...