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Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... MPCs were cultured at 37°C and 5% CO2 in Hams F-12 Nutrient Mixture (Thermo Fisher, Waltham, MA) supplemented with 20% FBS (Biotechne ...
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bioRxiv - Genomics 2023Quote: ... Fragmented DNA was purified using ZYMO DNA Clean & Concentrator™-5 kit and quantified using Qubit fluorometer (Life Technologies). Purified DNA was serial diluted with healthy donor cfDNA to generate libraries from 100% ...
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bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
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bioRxiv - Immunology 2023Quote: ... counted and 5×105 cells were replated in 350 µl in complete glucose-free RPMI (11879020, Thermo Fisher Scientific), supplemented with 100 U/mL penicillin ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco) at 1:8 beads:T cell ratio ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated at 37 °C in 5% CO2 in high glucose Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies), supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Gibco, 11965092; 10% FBS, heat-treated at 56C for 30 minutes, Gibco, 26140079; 5% penicillin-streptomycin 10,000U/mL, Gibco, 15140122 ...
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bioRxiv - Pathology 2023Quote: Primary human lung fibroblasts (n = 6 for both control and IPF, all at passage 5) were harvested using 0.25% Trypsin-EDTA (Gibco) and centrifuged at 500 x g for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Gibco, 11965092; 10% FBS, heat-treated at 56C for 30 minutes, Gibco, 26140079; 5% penicillin-streptomycin 10,000U/mL, Gibco, 15140122 ...
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bioRxiv - Cancer Biology 2023Quote: ... containing 5% β-Mercaptoethanol for 10 minutes at 70°C and loaded to a 10% NuPAGE gel (ThermoFisher, NP0301BOX). After electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: EdU (5-ethylnyl-2’-deoxyuridine) was prepared according to the manufacturer’s recommendations (10mM stock solution in DMSO, ThermoFisher #C10337). This solution was then diluted in sterile PBS to achieve a final concentration of 5mg/kg body weight of the pup when injected ...
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bioRxiv - Zoology 2023Quote: Three biological replicates of 3rd instar larvae and adults were collected in pools of 10 and 5 respectively in 1000μL TRIzol Reagent (Invitrogen) then RNA extracted following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with cold PBS and chromatin was harvested by scraping the cells in Farnham lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40) supplemented with protease inhibitors (Thermo Fisher). Chromatin immunoprecipitation was performed as previously described [33] using an anti-FLAG M2 antibody (Sigma-Aldrich F1804 ...
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bioRxiv - Bioengineering 2023Quote: ... with a multiwavelength UV-Vis photodetector and a C18 Hypersil BDS column (4.6 x 150 mm, 5 µm, ThermoFisher). The chromatographic analysis was carried out using mobile phase A (0.1 % TFA in water ...
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bioRxiv - Physiology 2023Quote: Amplicons for dsRNAs were designed using the SnapDragon dsRNA design tool (https://www.flyrnai.org/snapdragon) and in vitro transcribed (IVT) using MEGAscript® T7 Transcription Kit (Cat# AMB1334-5, ThermoFisher). IVT reactions were carried out as per the protocol provided by the DRSC (available at ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 5% FBS and antibiotics (penicillin 100 U ml−1, streptomycin 100 U ml−1 (pen/strep) (Gibco), 10mM glutamax (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... The established stable cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque) supplemented with 4∼5% fetal calf serum (FCS; Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample was washed 3 x 5 min with PBS and mounted in Fluoromount-G (ThermoFisher, 00-4958-02). 15 samples per genotype were prepared and photographed using a tile scan at a confocal microscope Zeiss LSM780 (Zeiss ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were grown on gelatinised culture plate at 37C and 5% CO2 in medium containing Dulbecco’s Modified Eagle Medium (Gibco) with 10% foetal bovine serum (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... and lectin microarrays) were carried out in the same mediums with 5% dialyzed Fetal Bovine Serum (FBS) (Gibco, A3382001) replacing the standard HS used for MCF10A culture ...
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bioRxiv - Cell Biology 2023Quote: ... boiled at 95°C for 5 min and loaded into a NuPAGE 3-8% Tris-Acetate Gel (Thermo Scientific) along with HiMark™ Pre-stained Protein Standard (Thermo Scientific LC5699) ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with DAPI (1/25,000 in PBS) for 5 min and mounted with Prolong Diamond antifade mounting medium (Invitrogen). The samples were imaged and analyzed with C-Plan Apochromat 63x/1.4 Oil DIC M27 objective under confocal microscope (LSM780NLO ...
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bioRxiv - Cancer Biology 2023Quote: ... cultured at 37°C in a humid 5% CO2 atmosphere in either the RPMI 1640+GlutaMAX medium (Life Technologies) supplemented with 10% fetal bovine serum and 2 nM DHT (AD-cells ...
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bioRxiv - Biochemistry 2023Quote: ... citri inocula OD600nm was normalized to 0.05 and incubated for 5 days at 30°C in a laminated microscopy chamber (Nu155411; Lab-Tek, NUNC). Biofilm images were acquired using a Nikon Eclipse Ti microscope equipped with a 100x magnification objective (CFI Plan Apo Lambda 100XH ...
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bioRxiv - Biophysics 2023Quote: ... The resulting cell pellets were resuspended in FACS buffer (PBS supplemented with 0.5% (w/v) BSA and 0.1% (w/v) sodium azide and counted using an Attune NxT Flow Cytometer (ThermoFisher). Gating for live cells with moderate levels of the GFP were used to compare MHC-I expression ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 1 μg of peptides were loaded on a C18 precolumn (PepMap100, 5 μm, 300 Å, Thermo Fisher Scientific) and separated over a C18 column (50 cm × 75 μm ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: 5 µg of total RNA was used for poly(A)+ RNA purification with MicroPoly(A)Purist™ Kit (Ambion). Poly(A)+ RNA was fragmented at 70°C for 10 min in RNA Fragmentation buffer (Ambion) ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was then performed using EvaGreen Master Mix (Solis BioDyne) detected by the QuantStudio 5 and analyzed using QuantStudio Software v1.5.2 (Applied Biosystems). Primer sequences used for qRT-PCR are shown in supplementary materials and methods ...
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bioRxiv - Molecular Biology 2023Quote: ... concentrated over an Acclaim PepMap C18 pre-column (5µm particle size, 0.3mm ID x 5 mm length, ThermoFisher Scientific), then bound to an EasySpray C18 column (2 µm particle size ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked with 5% bovine serum albumin in TBST and biotin was detected with Streptavidin-HRP (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... Metabolites were detected and quantified as area under the curve based on retention time and accurate mass (5 ppm) using the TraceFinder 4.1 software (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... IL-5 and IL-13 concentration were assessed following manufacturer’s instructions using mouse uncoated IL-5 and IL-13 ELISA kits (Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... Fibroblasts were cultured at 37° C at 5% CO2 in Dulbecco’s modified Eagle’s medium with nutrient mixture F-12 (Gibco-11330) and supplemented with 15% fetal bovine serum ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... sections were washed three times for 5 min in PBS (0.1 M, pH 7.4) and incubated with rabbit anti-GFP (1:500; Invitrogen, A11122) and chicken anti-GFAP (1:500 ...
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bioRxiv - Genomics 2023Quote: ... HB-8065) were cultured in a humidified incubator at 5% CO2 at 37 C using high-glucose DMEM (Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized and quantitative PCR performed using SYBR Green Express and a QuantStudio 5 qPCR instrument (Life Technologies). Primers were:
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bioRxiv - Microbiology 2023Quote: ... or BSA (control, 5 µg/mL, Roth, Karlsruhe, Germany) in colorless RPMI (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) for 2 h at 37 °C ...
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bioRxiv - Genomics 2023Quote: cDNA was generated from 5 μg of RNA per sample using the SuperScript IV First-Strand Synthesis System (Invitrogen). A VHL-specific primer complementary to the gene’s 3’ UTR was used for priming the reaction ...
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bioRxiv - Immunology 2023Quote: ... and cellular debris spun down at 12,000 g for 5 minutes before being subjected to electrophoresis on Bolt 4- 12% Bis-Tris gels from Invitrogen/Thermo Fisher (Cat ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed extensively (3 x 5 mins) in TBS-tween20 and incubated in appropriately labeled secondary antibodies (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Following preset incubation times (0, 0.5, 1, 3, or 5 h) cells were harvested by trypsinization (500 μl TrypLE, Gibco) for 5 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... washed in 1xTBS and then incubated for 5 min with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher, 34577). The chemiluminescent signal was scanned with UVP ChemStudio (Analytik Jena) ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: HEK293 (ATCC CRL-1573) and HEK239A ΔGRK2/3/5/646 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco 1196511) supplemented with 10% FBS (Hyclone SH30910.03) ...
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bioRxiv - Neuroscience 2023Quote: ... Zebrafish embryos were injured at 3 dpf and placed in 5 ug/mL acridine orange (ThermoFisher, Cat. No. A1301) diluted in egg water for 20 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 µL 40 µg/µL of each DSP was combined with 45 µL DNA coating solution (17250, ThermoFisher Scientific) and added to six wells of a medium-binding microplate (655076 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 µL of total mRNA was converted to total cDNA using random hexamers following manufacturer’s instructions (Verso, Life Technologies).
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bioRxiv - Immunology 2023Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... 5 µg of each protein was injected on to a 2.1 × 30 mm Poros R2 column (Thermo Fisher Scientific) and resolved using the following rapid gradient ...