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Citations for Thermo Fisher :
8701 - 8750 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and rho kinase inhibitors such as a CEPT cocktail consisting of 5 µM Emricasan (50-136-5235, Fisher Scientific), 50 nM Chroman 1 (7163 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with Alexa488-labelled goat anti-mouse IgG (A11001, Thermo Fisher Scientific, 5 μg/mL, 45 min, RT), and titres were calculated by the modified Kärber method ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplification reactions were monitored for 5 hours at 30 °C in MicroAmp fast reaction tubes with cap (Applied Biosystems) and using real-time PCR machine (Applied Biosystems).
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bioRxiv - Molecular Biology 2023Quote: ... Human dispersed islets were seeded at a density of 5×104 cells/cm2 on ultra-low attachment plates (ThermoFIsher) and allowed to form spontaneous pseudoislets ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting single cell suspension was washed three time with 5 ml of warm Neurobasal Plus medium (Gibco, A3582901) containing B27 Plus supplement (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... were maintained at 37°C in 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Thermo Fisher), 2 mM Glutamax ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 5 hrs before the media was changed to media + 1% Calcein AM stain (Thermo Fisher) and incubated for a further hr ...
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bioRxiv - Bioengineering 2023Quote: ... qPCR was performed with PowerTrack SYBR green master mix (A46109) by using QuantStudio™ 5 qPCR system (ThermoFisher, A34322) with specific primers (Table 2) ...
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bioRxiv - Microbiology 2023Quote: ... 20 μl resuspended parasite culture were incubated with dihydroethidium (5 μg/ml, Cayman) and SYBR Green I dye (0.25 x dilution, Invitrogen) in a final volume of 100 μl medium for 20 min at room temperature protected from light ...
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bioRxiv - Molecular Biology 2023Quote: ... scraped into 6 ml Farnham lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% Igepal CA-630) with protease inhibitors (ThermoFisher, # A32953), transferred to 15 ml Falcon tubes ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies were prepared in 5% goat serum in blocking buffer: goat anti-mouse 594 (AlexaFluor Invitrogen, 1:2000) and goat anti-rabbit 488 (AlexaFluor Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... the slides were directly subjected to background blocking using the Ultra Vision Protein Block for 5 min (ThermoFisher Scientific) and exposed to primary and secondary antibodies for 45 min and 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... were routinely cultivated at 37°C with 5% CO2 in Dulbecco’s modified Eagle medium (DMEM; Gibco, Dun Laoghaire, Ireland) supplemented with 10% FBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were kept in culture at 37°C and 5% CO2 and split twice per week by trypsinization using 0.05% trypsin EDTA (Gibco) followed by passage at 1:5 or 1:10 into a new tissue culture (TC)–treated T75 flask (Thermo Scientific ref 156499) ...
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bioRxiv - Microbiology 2023Quote: HEK 293T/17 cells and Vero-E6 cells were cultured at 37 °C and 5% CO2 in growth medium containing high glucose Dulbecco’s modified eagle medium (Gibco) supplemented with 10% calf serum (Hyclone) ...
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bioRxiv - Immunology 2023Quote: ... 100mL cultures of Expi293F cells at a density of 2.5×106 cells/mL were transiently transfected with 50μg each heavy and light chain encoding plasmids and Expifectamine (Invitrogen) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from fly heads was extracted from 5-day-old female flies using the Trizol reagent (Invitrogen, USA), mRNA was purified from total RNA using oligo(dT)-attached magnetic beads ...
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bioRxiv - Neuroscience 2022Quote: Human SH-SY5Y neural cells and HEK-293T cells were maintained at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
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bioRxiv - Neuroscience 2023Quote: ... animals were injected intraperitoneally with 5-ethynyl-2’-deoxyuridine in sterile saline (EdU, Thermo Fisher Scientific, USA, catalog #A10044), at a dose of 50mg/kg mouse on post-MCAO day 3-7 in single daily injections ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were loaded with 5 μM Fura2-AM and 400 ng/mL Pluronic F-127 (Thermo Fisher Scientific, USA) in control external solution for 45 min at 37 °C in 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5% CO2 and then fixed and permeabilized using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen, 00-5523). Cells were stained with primary and secondary antibodies as described previously for immunofluorescence ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... K18 tau and αSyn fibrils were diluted to 2.5-10 µM in 5 mM acetate buffer (Fisher Scientific, Switzerland) at pH 4.5 and coupled for 750 s at a flow rate of 5 µL/min on all surfaces except for ZD150D ...
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bioRxiv - Neuroscience 2022Quote: ... Background staining was blocked in 5% serum and incubated with either an anti-MHCII (1:500, M5/114.15.2; Thermo Fisher) or a pSer129 (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: Forty micrograms of protein was heated in the loading buffer and loaded on 5–12% gradient NuPage gels (Invitrogen). After the transfer on nitrocellulose membranes ...
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bioRxiv - Genomics 2022Quote: ... The flow-through was centrifuged and resuspended in 5 ml of red blood cell lysis buffer (Invitrogen, 00-4300) for 10 min.
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bioRxiv - Genomics 2022Quote: ... The flow-through was centrifuged and resuspended in 5 ml of red blood cell lysis buffer (Invitrogen, 00-4300) for 10 min ...
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bioRxiv - Genomics 2022Quote: ... The fixed cells were centrifuged at 2,500g for 5 min to discard the supernatant and washed by 0.5 mL PBS (Gibco, #20012027) once.
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bioRxiv - Molecular Biology 2022Quote: ... 5 days after electroporation cells were collected and subjected to flow cytometry with an Attune Flow Cytometer (ThermoFisher Scientific). Downstream analysis was performed using FlowJo Software v10.8.2 (FlowJo ...
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bioRxiv - Immunology 2023Quote: ... T-cells were maintained at 37°C in an atmosphere of 5% CO2 in 1640 RPMI media (Life technologies) supplemented with 100 µg ml-1 penicillin (Life technologies) ...
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bioRxiv - Immunology 2023Quote: ... Slides were then washed in 1X PBS three times for 5 minutes and incubated with a secondary antibody (Invitrogen Goat-anti-rabbit 594 ...
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bioRxiv - Biophysics 2023Quote: ... their chest was cut and intra-cardiac injection was performed with 5 ml solution of Dil stain (ThermoFisher D282) to label the luminal side of blood vessels ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were online desalted on a trapping cartridge (Acclaim PepMap300 C18, 5 μm, 300Å wide pore, Thermo Fisher Scientific) for three minutes using 30 uL/min flow of 0.05% TFA in water ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 5 × 105 KP or LLC cells suspended in 450 µl Dulbecco’s phosphate-buffered saline (PBS, Gibco) were injected subcutaneously into the flanks of MPO-KO and WT mice aged 6–14 weeks ...
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bioRxiv - Bioengineering 2022Quote: ... organoids were washed 3 times for 5 minutes each using PBS and mounted on glass microscope slides (Fisher Scientific). 90 μm Polybead Microspheres (Polyscience ...
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bioRxiv - Microbiology 2022Quote: ... the sample was drawn off and the grids were rinsed with 5 μL of nuclease-free water (Invitrogen AM9938). The water was then drawn off and the rinse was repeated ...
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bioRxiv - Genomics 2023Quote: ... The samples were further post-fixed with 1.5 mM BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate) (Thermo Scientific A35396) in 1× PBS at room temperature for 20 minutes and quenched with 100 mM Tris-HCl pH7.4 (Alfa Aesar J62848 ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were resuspended for a final concentration of 50,000 cells per 5 μL in Neuronal media supplemented with 1% anti-anti (GIBCO), 5% KSR ...
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bioRxiv - Microbiology 2023Quote: ... A549 cells (a human adenocarcinoma epithelial cell line) were cultured at 37°C under 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 1% penicillin-streptomycin (Pen-Strep ...
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bioRxiv - Biophysics 2023Quote: ... followed by 5 min UV treatment and 3x washing with phosphate buffered saline (Gibco PBS, Thermo Fisher Scientific, Waltham). Next ...
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bioRxiv - Biophysics 2023Quote: ... followed by 5 min UV treatment and 3x washing with phosphate buffered saline (Gibco PBS, Thermo Fisher Scientific, Waltham). Next ...
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bioRxiv - Cell Biology 2023Quote: ... tissues embedded in paraffin were cut into 5 µm thick sections with an automated microtome (HM355S, Thermo Fisher Scientific). After deparaffinization ...
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bioRxiv - Cell Biology 2023Quote: ... the former growth medium with the fetal calf serum replaced by 5% horse serum (Gibco/Thermo Scientific, Waltham, USA). The used horse serum was from a batch that had been tested regarding muscle cell differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... the former growth medium with the fetal calf serum replaced by 5% horse serum (Gibco/Thermo Scientific, Waltham, USA). The used horse serum was from a batch that had been tested regarding muscle cell differentiation ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 uL proteinase K mix from the ARCTURUS® PicoPure® DNA extraction kit (Applied Biosystems, Cat no. KIT0103) was added to each sample and they were incubated for 30 min at 65°C followed by 5 min at 95°C ...
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bioRxiv - Genomics 2023Quote: Confluent low-passage human lung epithelial A549 cells were obtained from ATCC and grown at 37°C in 5% CO2 and in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Gibco/Life Technologies) supplemented with 10% fetal bovine serum (Omega Scientific) ...
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bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody incubation was performed overnight at 4°C in PBST with 5% normal goat serum (Thermo Fisher, USA). Secondary antibodies were diluted in PBST and incubated for 1 hr at room temperature ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum and activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) at a 1:1 bead:cell ratio according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Intracellular staining was performed by permeabilization for 5 minutes on ice with 0.5% saponin in PBA-E (PBA-S) or permeabilization buffer (ThermoFisher) and subsequent staining with antibodies diluted in PBA-S ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were washed in DPBS for 5 min three times and then incubated with DAPI (Thermo Fisher, 62248) at 1:1000 dilution in 2× SSC (diluted from 20× SSC ...