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Citations for Thermo Fisher :
8651 - 8700 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... BHK21 and Vero E6 cells were maintained at 37°C in a humidified atmosphere containing 5% CO2 and cultured in Dulbecco’s Modified Eagle medium (DMEM; Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 8-10 times in PBS for 5 minutes and incubated with DAPI (0.1 mg/ml in PBS; #62248, Invitrogen) to stain nuclei ...
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bioRxiv - Physiology 2024Quote: ... Transfections were performed for 5 h in OptiMEM reduced serum media with Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA). Terminal experiments were performed ≈48 h after the second transfection.
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR reaction and data collection was performed using the QuantStudio 5 Real-Time PCR System (Applied Biosystems, USA) and raw data exported to Microsoft Excel for analysis ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were permeabilized and nonspecific binding blocked with PBS containing 0.5% TritonX100 (#T8655, US Biological) and 5% goat serum (#16210-072, Gibco) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: HeLa cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) with Glutmax and 5% FCS (Gibco) and passaged every few days ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... Cells were cultured at 37C at 5% CO2 in Advanced Dulbecco’s Modified Eagle Medium: Nutrient mixture F-12 (DMEM-F12) with Glutamax (GIBCO), supplemented with 12% FCS (Clontech) ...
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bioRxiv - Cell Biology 2024Quote: ... transient transfections of 5 μg of pcDNA3.1-Foxm1 were performed using 25 μg of Lipofectamine 2000 (Thermo Fisher Scientific). After 24 h ...
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bioRxiv - Cell Biology 2024Quote: HeLa (ATCC CCL-2) cells were grown at 37 °C and 5% CO2 in Dulbecco’s modified Eagle medium (DMEM, GIBCO) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2024Quote: ... Tissues were rehydrated with PBS and blocked for 1 hour at room temperature with 5% normal goat serum ((Gibco) source ...
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bioRxiv - Genomics 2024Quote: ... 5-10 μg of antibody was coupled to 50-100 μl of Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR was performed using the SYBR Select Master Mix and a QuantStudio 5 Real-Time PCR System (Applied Biosystems). Primers against specific target genes are listed in Supp ...
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bioRxiv - Immunology 2024Quote: ... and the RT-PCR reaction was performed using a Quant Studio 5 Real-Time PCR Instrument (Applied Biosystems, MA). For quantification ...
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bioRxiv - Genetics 2024Quote: ... Cells were cultured at 37C at 5% CO2 under humidity in myoblast proliferation medium [1Vol medium 199 (ThermoFisher, 41150020) + 4 vol DMEM (ThermoFisher ...
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bioRxiv - Developmental Biology 2024Quote: ... at a ratio of 5 uL Lipofectamine™ 2000: 2.5 ug plasmid DNA diluted in Opti-MEM (Gibco: 31985062). After 48 hrs ...
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bioRxiv - Cell Biology 2024Quote: ... and aliquots containing 5 × 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen). The sample lane was divided into eight slices that were excised from the Coomassie-stained gel ...
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bioRxiv - Microbiology 2024Quote: ... were then mixed 1:1,000 with 5 mL of MMB agar + Tetracycline and poured into 4-well Nunc Rectangular Dishes (ThermoFisher). Dishes were dried at room temperature for 1 hour ...
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bioRxiv - Microbiology 2024Quote: Both 3D7 and IT4 parasite lines were maintained following standard procedures at 5% hematocrit in RPMI 1640 medium supplemented with 0.5% Albumax II (Invitrogen) in an atmosphere containing 5% oxygen ...
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bioRxiv - Microbiology 2024Quote: ... RAW 264.7 cells were seeded at a density of 5 × 105 cells/well in 24-well tissue culture plates (ThermoFisher) for bacterial uptake assay and in 12-well plates with round coverslips for immunofluorescent microscopic analysis ...
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bioRxiv - Microbiology 2023Quote: ... Samples were washed three times with PBS and nuclei were stained for 5 min with Hoechst 33342 (Life Technologies) in PBS ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 40 µg of RNA were treated with 5 U of DNase I (Fermentas) and 0.5 µL of Superase-In RNase Inhibitor (Ambion) for 45-60 min at 37°C in 50 µL reaction volumes ...
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bioRxiv - Microbiology 2024Quote: ... DAPI staining was performed for 5 min in PBS 1X to reveal the nuclei (D1306, Invitrogen, Thermo Fisher Scientific). Slides were mounted on coverslips with Fluoromount-G mounting media (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... DAPI staining was performed for 5 min in PBS 1X to reveal the nuclei (D1306, Invitrogen, Thermo Fisher Scientific). Slides were mounted on coverslips with Fluoromount-G mounting media (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... falciparum (3D7, Dd2, and HB3) parasites were cultured in 3-5% human O+ RBCs in RPMI-1640 (Gibco, 110875093) complete medium containing 0.5% Albumax-II (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Colon was further cut into 5-10 mm pieces and put into a 15 mL falcon tube with 5 mL prewarmed 2 mM EDTA in Hank’s balanced salt solution (HBSS, Gibco) and shaken at 250 rpm for 15 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were trapped on a C18 Acclaim PepMap 100 (5 µm, 300 µm x 5mm) trap column (ThermoFisher Scientific) and eluted onto a C18 Acclaim PepMap100 3 µm ...
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bioRxiv - Immunology 2024Quote: ... comprising 2 μl sample DNA and 48 μl reaction mixture containing per reaction 5 μl PCR buffer 10x (Invitrogen), 2 μl MgCl2 (50 mM) ...
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bioRxiv - Genomics 2024Quote: ... St Jude Children’s Research Hospital) cells were cultured in culture medium F12 media with 2.5% or 5% heat inactivated fetal bovine serum (GIBCO) and Penicillin-Streptomycin (GIBCO) ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed with PBS-0.1%Tríton-X three times (5 minutes each) and incubated with the secondary antibodies (1:2000; Alexa-Fluor conjugated; Invitrogen), at RT for 1h ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA of tooth germs (E15.5 cultured for 5 days) were extracted by using TRIzol™ Reagent (Invitrogen, USA) according to instruction of manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were cultured in 6-well plates at 37 °C in a 5% CO2 atmosphere and split every 72 hours by trypsinization (0.25%, Gibco, ThermoFisher). Stable HEK293 lines were generated by lipofection (Lipofectamine 2000 ...
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bioRxiv - Molecular Biology 2023Quote: The cDNAs encoding MpRBOHB and its variants with 3 × FLAG tag at their 5’-end were cloned into the pcDNA3.1(-) vector (Invitrogen) for the quantitative measurement of ROS in HEK293T cells.
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with secondary goat anti-rabbit antibodies (dilution 1:1000 in TBST/5% milk; Invitrogen, 32460) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in keratinocyte serum-free medium supplemented with 0.05 mg/ml bovine pituitary extract and 5 ng/ml human recombinant epidermal growth factor (Gibco). T24 human bladder cancer cells were cultured in HAM’s F12 medium supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin/glutamine (PSG) ...
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bioRxiv - Cell Biology 2023Quote: MDCK strain II cells were cultured at 37°C with 5% CO2 in high-glucose Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen). The medium was supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the media was replaced with Opti-MEM containing Ambion Silencer-Select ERK3 siRNA (5 pmol, catalog number: 4390771 ID: S78451) and Lipofectamine 2000 (2µl, Invitrogen) and cells incubated for 19 h ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from 5×107 promastigotes with the help of the PureLink RNA mini kit (Invitrogen, USA). The RNA was treated with DNaseI (1U DNase I per 2 μg RNA at 37°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed briefly with PBS and mounted in 5 µL ProLong Gold Antifade mounting medium (Thermo Fisher Scientific). Cells were imaged at room temperature using a confocal laser scanning microscope (Zeiss LSM800 ...
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bioRxiv - Genetics 2023Quote: ... mice were overdosed with inhaled isoflurane (>5%) and transcardially perfused with 4°C Ca2+/Mg2+-free 1X HBSS (Invitrogen). Bilateral T11-L2 DRGs ...
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bioRxiv - Developmental Biology 2023Quote: Lateral wall explants were immersed for the indicated times at 37°C with 5% CO2 in DMEM-Glutamax (Invitrogen) supplemented with 10% FCS ...
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bioRxiv - Immunology 2023Quote: ... The paraffin blocks were sliced into 5 to 6 μm sections using a Microm HM 325 microtome (Thermo Scientific), and the sections were stained with Hematoxylin and Eosin (H&E) ...
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bioRxiv - Immunology 2023Quote: ... IL-5 and IL-13 concentration were assessed following manufacturer’s instructions using mouse uncoated IL-5 and IL-13 ELISA kits (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... cells were suspended in 200 μl of FACS buffer and acquired on Bigfoot 5-laser Spectral Cell Sorter (Invitrogen). Acquired samples were unmixed using Bigfoot Sasquatch software and analyzed with FlowJo software v10.9 (Tree Star Inc.).
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bioRxiv - Pharmacology and Toxicology 2023Quote: Human Embryonic Kidney (HEK)293T cells (ATCC) were grown at 37°C and 5% CO2 in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal bovine serum (Bodinco and Gibco) ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were maintained in high glucose Dulbecco’s modified Eagle’s medium 5 g/L glucose (DMEM; Gibco, UK, 11965084). Cell culture media were supplemented with 10% (v/v ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were kept in culture at 37°C and 5% CO2 and split twice per week by trypsinization using 0.05% trypsin EDTA (Gibco) followed by passage at 1:5 or 1:10 into a new tissue culture (TC)–treated T75 flask (Thermo Scientific ref 156499) ...
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bioRxiv - Microbiology 2023Quote: HEK 293T/17 cells and Vero-E6 cells were cultured at 37 °C and 5% CO2 in growth medium containing high glucose Dulbecco’s modified eagle medium (Gibco) supplemented with 10% calf serum (Hyclone) ...
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bioRxiv - Immunology 2023Quote: ... 100mL cultures of Expi293F cells at a density of 2.5×106 cells/mL were transiently transfected with 50μg each heavy and light chain encoding plasmids and Expifectamine (Invitrogen) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from fly heads was extracted from 5-day-old female flies using the Trizol reagent (Invitrogen, USA), mRNA was purified from total RNA using oligo(dT)-attached magnetic beads ...
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bioRxiv - Neuroscience 2022Quote: Human SH-SY5Y neural cells and HEK-293T cells were maintained at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...