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Citations for Thermo Fisher :
8601 - 8650 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Slides were rinsed 3 x 5 min and cell nuclei were labeled by DNA staining using Hoechst (Life Technologies) for 30 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... A μ-Precolumn C18 PepMap100 trap column (5 μm, 300μm i.d., 5mm length, 100 Å; Thermo Fisher Scientific, USA) and a self-packed analytical column (XBridge Premier Peptide BEH C18 ...
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bioRxiv - Neuroscience 2023Quote: ... Cultures were maintained in a humified incubator at 37 °C and 5% CO2 in Neurobasal medium (Gibco™ 21103049) supplemented with B27 (Gibco™ 17504044) ...
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bioRxiv - Microbiology 2022Quote: ... One ml of fd or Pf4 phage (5 mg/ml) was incubated with 100 µg A488 fluorescent dye (ThermoFisher) for 1 hour at room temperature (RT ...
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bioRxiv - Biochemistry 2022Quote: ... cells were maintained in a humidified 37° C incubator with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, MilliporeSigma) supplemented with 2 mM L-Glutamine (L-Glu, Gibco), 0.1 mM non-essential amino acids (NEAA ...
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bioRxiv - Physiology 2022Quote: ... Cells were grown in DMEM-F-12 (D6421) supplemented with 5% FBS (GIBCO/Thermo Fisher Scientific, Grand Island, NY) and maintained at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... ACC 57) were cultured at 37°C and 5% CO2 in high glucose Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal calf serum (Linaris) ...
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bioRxiv - Neuroscience 2022Quote: ... Eyes were intravitreally injected using a glass micropipette with 1-5 μl of 0.2% cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 or 555 (Invitrogen) diluted in 1% DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... NSCs were seeded at a density of 5 × 104 cells/cm2 and precoated with Geltrex (A1413301, Thermo Fisher Scientific) in NSC expansion medium ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were loaded with 5 µM calcium-sensitive fluorescent dye Fluo-4AM (Direct Calcium Assay Kit, Invitrogen, USA) for 30 min at 37° C and washed for 15 min or 30 min at 37° C and then for 5 min at RT in basic solution (BS ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 ug of psPAX2 and 5 ug pMD.G plasmid were vortexed with 40 ul P3000 reagent in 1.5 ml OptiMEM (ThermoFisher, 31985070). Then 40 ul Lipofectamine was added to 1.5 ml OptiMEM and briefly vortexed ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody diluted in 5% NGS in PBS (β-catenin 1:200, ProteinTech, rhodamine-phalloidin 1:200, Molecular Probes) for 36 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM EDTA) in the presence of one tablet of the protease inhibitor cocktail per 25 ml (Complete, Invitrogen). Cell lysates were analyzed by 12% SDS-PAGE for 80 min at 100 V (Mini-Protean Cell ...
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bioRxiv - Immunology 2022Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated for 5 minutes in the dark with SuperSignal™ West Pico Plus substrate ((Invitrogen, 34577) was used for actin and Tuj1 detection ...
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bioRxiv - Physiology 2022Quote: Adipocyte differentiation was induced by treating confluent cells for 2 days with 5 mg/ml insulin (Thermo Fisher Scientific) and 10μM ETYA (Santa Cruz Biotech ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa S3 cells (ATCC, CCL-2.2) were maintained at 37°C and 5% CO2 in DMEM medium (ThermoFisher, 11995073) containing 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: K562 cells (ATCC, CCL-243) were maintained at 37°C and 5% CO2 in RPMI 1640 medium (ThermoFisher, 11875119) containing 10% FBS (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... 3.125 µM Oligo-dT30VN (IDT, 5′-AAGCAGTGGTATCAACGCAGAGTACT30VN-3′) and 1:600,000 ERCC RNA spike-in mix (Thermo Fisher, 4456740)) into 384-well hard-shell PCR plates (Biorad HSP3901 ...
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bioRxiv - Microbiology 2022Quote: The maleimide labeling of the HR2 peptides was performed by mixing 100 µM peptide and 2.5 mM fluorescein-5-maleimide (ThermoFisher) in degassed buffer (50 mM sodium phosphate ...
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bioRxiv - Bioengineering 2022Quote: Samples were embedded in paraffin and 5 µm thick sections were prepared using a microtome (Microm, HM430, Thermo Scientific). Safranin-O ...
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bioRxiv - Molecular Biology 2022Quote: 1-5 × 107 parasites were harvested by centrifugation and RNA was extracted from cells using Trizol (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides were loaded onto a C18 trap column (Acclaim PepMap, 100 A 5 µm particle size, Thermo Fisher Scientific) at a flow rate of 5 µL/min in Solvent A (0.1% formic acid in water ...
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bioRxiv - Cell Biology 2022Quote: ... cells (5×106/0.5 ml) were labeled with 100 μg Immunopure Sulfo-NHS-LC-Biotin (Thermo Scientific, Rockford, IL) for 30 min at 22 °C ...
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bioRxiv - Cell Biology 2022Quote: ... were maintained as a confluent culture at 37°C and 5% CO2 in DMEM (4.5 g/L glucose, Gibco) supplemented with 10% heat- inactivated FCS ...
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bioRxiv - Microbiology 2024Quote: ... Confluent TRF cells were infected with 1 MOI FL-RhCMV in low serum (5%) medium in T75 flasks (ThermoFisher). Mock infection was performed in parallel ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were heated to 80°C for 5 min and electrophoresed on 4-12% NuPAGE Bis-Tris gels (ThermoFisher). Wet transfer was to a nitrocellulose membrane (0.45 uM pore ...
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bioRxiv - Biochemistry 2024Quote: ... were obtained from Janvier and infected with 5×103 AnTat1.1E trypanosomes (intraperitoneally (i.p.) in 200 µL HBSS (Hanks’ balanced salt solution, ThermoFisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... and forward/reverse primers from IDT Technology in the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems; A28574). The cycle was run 40 times at 95°C denaturation for 30 sec ...
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bioRxiv - Biochemistry 2024Quote: To perform DSF, 5 µM protein was mixed with substrate (0, 1, or 15 mM) and 2.5x SYPRO Orange dye (ThermoFisher), and made up to a total volume of 50 µl with DSF buffer (50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... 2,000 cells were dispensed in 5 μl of medium using Multidrop Combi (Thermo Fisher Scientific Inc., Waltham, MA, USA). The final concentration of the screened compounds was 10 μM ...
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bioRxiv - Bioengineering 2024Quote: ... and 72°C extension for 30 sec by using QuantStudio™ 5 Real-Time PCR System (A28574, Applied Biosystems). The expression levels of targeted genes were normalized using the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Cell Biology 2024Quote: ... half of the media was replaced with fresh HoxB8 Media Culture containing 5 µg/mL blasticidin-HCl (ThermoFisher A1113903) for selection ...
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bioRxiv - Cell Biology 2024Quote: ... 0,5µM Smartseq3 OligodT30VN (IDT; 5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’) adjusted to RT volume and 0,5mM dNTPs/each (Thermo Fisher, #R0181). After cell sorting lysis plates were centrifuged before storage at -80°C ...
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bioRxiv - Bioengineering 2024Quote: ... and forward/reverse primers from IDT Technology in the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems; A28574). The cycle was run 40 times at 95°C denaturation for 30 sec ...
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bioRxiv - Bioengineering 2023Quote: ... 5 × 105 HSkMC cells were incubated with 2 μg of eGFP-mRNA packaged in LPP or lipofectamine (Thermo Scientific) for 24 h in 6-well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... and cell membranes were labeled with 5 µg/ml fluorescent disordered/nonraft phase marker FAST DiO (Thermo Fisher Scientific) for 10 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS (GIBCO, 14080-055) for 1h in 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Each peptide extract was loaded on a 300 μm ID x 5 mm PepMap C18 precolumn (Thermo Scientific, USA) at a flow rate of 10 μL/min ...
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bioRxiv - Cancer Biology 2024Quote: ... After 15 minutes of incubation with 5 nM SYTOX™ Red Dead Cell Stain (#S34859, Invitrogen, Carlsbad, CA, USA) on ice ...
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bioRxiv - Microbiology 2024Quote: ... BMDM were grown at 37°C in 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) GlutaMAX (Gibco #10569-010) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Molecular Biology 2024Quote: ... Ten microliter samples (up to 5 µg per sample) were labeled with 50 µg of TMT18plex (Thermo Fisher Scientific) reconstituted in 4 µL acetonitrile ...
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bioRxiv - Neuroscience 2024Quote: ... followed by centrifugation (5 minutes, 160 x g) and two rounds of trituration in wash buffer (DMEM Ham’s F-12, Gibco 10565-018 ...
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bioRxiv - Neuroscience 2024Quote: ... the blood plasma was labeled with a 100-μL retro-orbital injection of 5% W/V FITC-dextran dye (MW=70,000 kDa; Invitrogen), or QtrackerTM 655 (20 μL in 100 μL saline ...
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bioRxiv - Neuroscience 2024Quote: ... DRG neurons or HEK293 cells were incubated with 5 μM Fura-2 AM and 0.2% Pluronic (Thermo Fisher Scientific) (prepared from a 200 mg/ml stock solution in DMSO ...
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bioRxiv - Immunology 2024Quote: ... 20.000 BMDCs were co-cultured with purified T cells in a 1:5 DC:T cell ratio in sterile tissue culture medium (TCM; RPMI (GIBCO) containing 10% fetal calf serum (Bodinco) ...
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bioRxiv - Neuroscience 2024Quote: ... 5303 SPRN SPRN-5) was biotinylated using the EZ-Link NHS-PEG4 Biotinylation Kit (Thermo Scientific, catalog No. PI21455) and used at a concentration of 1µg/ml ...
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bioRxiv - Neuroscience 2024Quote: Cultured sensory neurons from wild-type and TRESK-/- mice were loaded with 5 μM fura-2/AM (F1221, Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... brains were first dissected in 1x PBS before incubating with 5 μM H2DCFDA (Thermo Fisher Scientific, Washington, DC; #D399) for 10 minutes at room temperature protected from light ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... The alkaline hydrolysis ladder was prepared by incubating 0.4 pmol 5’ end-32P-labeled RNA in 9 µL of 1x alkaline hydrolysis buffer (Ambion) for 5 min at 95°C ...