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Citations for Thermo Fisher :
7501 - 7550 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Approximately 30 μg of protein was subjected to gel electrophoresis and transferred to membrane using Bolt western blot reagents from ThermoFisher Scientific Inc (bolt 4-12% Bis Tris gel ...
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bioRxiv - Cell Biology 2021Quote: Protein lysates were obtained from all cell lines using RIPA buffer and separated in a NuPAGE Bis-Tris 4-12% gradient gel (ThermoFisher). Proteins were transferred to a nitrocellulose membrane ...
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bioRxiv - Immunology 2020Quote: ... Samples containing 30 µg/µl of cell lysate proteins per lane were resolved on SDS-PAGE (10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis) along with pre-stained protein ladder and transferred onto PVDF membranes (Invitrogen). The transferred membranes were blocked for 2 hours using 5% BSA in TBST (25 mm Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... The gel was transferred to a Nytran Supercharge membrane (Whatman) per manufacturer protocol using a TurboBlotter and NorthernMax Transfer Buffer (Ambion) for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... The purified amplicons were then inspected for quality using agarose gels and quantified using a Qubit 3.0 fluorometer (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... RNA quality and quantity were checked on a 1% agarose gel and with a NanoDrop spectrophotometer (ND-1000, ThermoFisher Scientific), respectively.
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bioRxiv - Neuroscience 2021Quote: ... a total of 5 ug of reduced protein lysates were loaded onto a 4-12% gradient Bis-Tris gel in 1X SDS Running buffer (Invitrogen) supplemented with the NuPAGE antioxidant ...
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bioRxiv - Plant Biology 2021Quote: ... RNA quality was determined via agarose gel electrophoresis and RNA purity and quantity was measured via NanoDrop 2000C (Thermo Scientific). 1000µg total RNA was used in iScript cDNA Synthesis (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... was separated on 4-12% Bis-tris SDS polyacrylamide gels (reducing) and then subjected to dry blot transfer onto PVDF membranes according to the manufacturer’s instructions (Life Technologies). The PVDF membrane was imaged using an Odyssey CLX system (Li-Cor Biosciences) ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 µl of cell lysates were separated on 4-12% tris-glycine SDS-polyacrylamide gel electrophoresis (SDS-PAGE) from Invitrogen, and transferred to methanol-soaked polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated for 10 min at 90°C and loaded on a 4–20% Tris-Glycine precast gel (ThermoFisher). Protein was transferred onto a nitrocellulose membrane using the iBlot Dry Blotting System ...
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bioRxiv - Cell Biology 2020Quote: ... Twenty μg of total protein extracts and 10 μg of nuclear extractions were loaded on 4-12% Bis-Tris gels (Invitrogen) in SDS-MES running buffer ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the GeneJET Gel Extraction Kit (ThermoFisherScientific, Vilnius, Lithuania) and quantified using a fluorometric RiboGreen kit (Qubit Assay Kit, Invitrogen, Life Technologies Corporation ...
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bioRxiv - Neuroscience 2021Quote: ... where a Tricaine-anesthetized zebrafish larva is trapped inside 2.5% low-melting agarose gel (UltraPure LMP Agarose, Invitrogen 16520-050). To facilitate the exposure of NECs to oxygen in the medium and observe larval movements ...
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bioRxiv - Immunology 2020Quote: ... 1μL aliquots were analysed by non-reducing SDS-PAGE electrophoresis on NuPAGE™ 12% Bis-Tris protein gels (ThermoFisher Scientific) to confirm the presence of non-aggregated HLA-E heavy chain and β2m.
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bioRxiv - Neuroscience 2021Quote: ... Indel mutations were confirmed by sequencing (Eurofins Genomics LLC, KY, USA) of gel-purified PCR products cloned into the pJet1.2 cloning vector (Life Technologies).
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bioRxiv - Biochemistry 2021Quote: ... The flow through was collected and analysed by SDS-PAGE (NuPAGE Bis-Tris 4-12% Pre-cast gels, ThermoFisher Scientific), concentrated using 10,000 molecular weight cut off (MWCO ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose membranes using an iBlot (Life Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... 75 μg of protein was loaded and samples were run on a NuPAGE 4-12% Bis-Tris Gel in MOPS SDS Running Buffer (Invitrogen). After separation by electrophoresis ...
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bioRxiv - Cancer Biology 2021Quote: ... 10-40 μg of proteins were loaded on a precast gel NuPAGE Novex 4-12% Bis-Tris (Thermo Scientific, NP0336BOX). Proteins were then transferred onto a nitrocellulose membrane using an iBlot2 dry blotting system with iBlot2 transfer stacks (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction products were then analysed by running on a 20% non-denaturing PAGE gel in 0.5X TBE buffer (Life Technologies) for 20 min at room temperature and 200 V ...
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bioRxiv - Biophysics 2021Quote: ... Gel bands were prepared for mass spectrometry by digesting them in 2 μg/band Trypsin/LysC protease mix (Thermo Scientific) for 16 hours at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... 15% polyacrylamide-gel electrophoresis (PAGE) gels in 1x Tris/Borate/EDTA (TBE) running buffer and were visualized by staining with SYBR Gold II (Invitrogen) stain.
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bioRxiv - Physiology 2021Quote: ... centrifuged for 1 min at 10’000 x g and 40 μg protein was loaded onto NuPAGE Bis-Tris 10% Protein Gels (ThermoFisher #NP0301BOX), and proteins were transferred to nitrocellulose membranes (Sigma #GE10600002) ...
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bioRxiv - Developmental Biology 2022Quote: ... ex vivo: 20 μg) of protein lysates as described above were resolved on 4- 12% Bis-Tris Protein gels (Invitrogen). Proteins were transferred to nitrocellulose membranes (Millipore ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and RNA fragments were recovered via ethanol precipitation and resuspended in 10 μl water and 10 μl Gel Loading Buffer II (Ambion). The OH ladder was generated by incubating the RNA in 1X Alkaline Hydrolysis Buffer (Ambion ...
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bioRxiv - Microbiology 2022Quote: ... and the aqueous layer was transferred to a 2 ml phase lock gel tube (QuantaBio) alongside an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1) solution (Invitrogen; 15593-031). Samples were centrifuged (18,000 x g for 5 min at 4°C) ...
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bioRxiv - Immunology 2022Quote: ... and 120 ug of protein were resolved on a gradient SDS-PAGE A total of 120 μg of protein from mixed lysates was migrated on a pre-cast 4-12% Novex NuPAGE Bis-Tris SDS Mini Gel (Invitrogen). After Coomassie staining ...
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bioRxiv - Microbiology 2022Quote: ... An equal amount (~25 μg) of the proteins were loaded on a NuPAGE 4-12% Bris–Tris gradient gels (Invitrogen) as previously described82 ...
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bioRxiv - Cell Biology 2022Quote: ... Protein purity was determined by resolving purified protein by SDS–PAGE using NuPAGE 4–12% Bis Tris precast gels (ThermoFisher) at 200 V ...
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bioRxiv - Developmental Biology 2022Quote: ... The quality of the gRNA prep was confirmed by agarose gel electrophoresis and quantified using a NanoDrop One (Thermo Fisher).
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bioRxiv - Plant Biology 2020Quote: ... the quality and quantity of the DNA was verified in 1.2 % agarose gel followed by quantitation using NanoDrop 1000 (Thermo Scientific). Genotyping of the DNA samples was performed through double digest restriction associated DNA (ddRAD ...
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bioRxiv - Plant Biology 2019Quote: ... The resultant PCR fragment was gel-purified and then recombined with pDONR 221 following the instructions of the Gateway BP Clonase II Enzyme mix (Invitrogen).
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bioRxiv - Microbiology 2019Quote: ... Equal protein amounts were run on 4-12% Bis/Tris gels in 1X NuPAGE MOPS SDS Running Buffer (ThermoFisher Scientific). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Synthetic Biology 2019Quote: ... benthamiana leaves infiltrated with SN14 (MTP-Su9::NifD::HA) were run on SDS-PAGE using a gel having a polyacrylamide concentration of 4-20 % (Invitrogen). The gel was stained with Aqua stain (Bulldog Bio) ...
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bioRxiv - Plant Biology 2019Quote: Blue native polyacrylamide gel electrophoresis (BN-PAGE) was performed using the Bis-Tris NativePAGE system from Invitrogen (Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... and then rotated for another 5 min before loading the strip into the well of a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen).
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bioRxiv - Molecular Biology 2019Quote: ... Ten micrograms of cell lysate or 20 µg of exosomal protein was separated on gradient SDS-polyacrylamide gel (4-12% Bis-Tris, Invitrogen) and transferred to nitrocellulose membrane (Trans-Blot Turbo ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantification was done using Pierce BCA protein assay (Thermo Fischer Scientific) and 20 µg were separated by NuPAGE 4-12% Bis-Tris gel (Invitrogen). Primary antibodies used were LAT1 (Cat #5347S ...
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bioRxiv - Genetics 2019Quote: ... 10 or 15μg of protein was loaded per well on Tris-Acetate 3-8% gels with Tris-Acetate running buffer (NuPAGE, ThermoFisher) for detecting KIF26B protein or on 4-12% NuPAGE Bis-Tris Gel with NuPAGE MES SDS Running Buffer (NuPAGE ...
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bioRxiv - Cell Biology 2020Quote: ... were denatured at 95°C for 5min and run on NuPAGE(tm) 4-12% Bis-Tris Midi Protein Gels (Invitrogen) at 200V for 1h ...
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bioRxiv - Molecular Biology 2019Quote: ... 3’-linked footprints were denatured at 80°C for 2 min and purified on 10% TBE-Urea polyacrylamide gels (Invitrogen) in 1x TBE (Ambion ...
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bioRxiv - Molecular Biology 2019Quote: ... The gel slurry was then transferred to a Spin-X cellulose acetate column (#60702, Thermo Fisher Scientific; Waltham, MA, USA) and centrifuged for 3 min at 20,000 × gav ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA quality was assessed using a NanoDrop (ThermoScientific) and by electrophoresis on 2% agarose gel containing ethidium bromide (Fisher Scientific).
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bioRxiv - Biochemistry 2019Quote: ... RNAs after hDcp2 treatment were resolved electrophoretically on denaturing 15% acrylamide / 7 M urea 1xTBE gel stained with SYBR Gold (Invitrogen) and visualized using a Typhoon FLA 9500 (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: Co-immunoprecipitation beads from two independent biological replicates were eluted in Laemmli buffer and run on a 4-12 % acrylamide gel (Invitrogen) and proteins were stained with Coomassie blue (Biorad) ...
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bioRxiv - Microbiology 2019Quote: Purified complexes and protein extracts were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on 4–12% NuPAGE Bis–Tris gels with MOPS running buffer and transferred to a nitrocellulose membrane (Invitrogen). Proteins were detected using standard immunoblotting techniques ...
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bioRxiv - Molecular Biology 2019Quote: ... and 25μg protein was loaded per lane onto a Bolt 4-12% 15-well Bis-Tris gel (Invitrogen, no. NW00105BOX). Protein transfer was performed using Thermo Fisher Scientific’s iBlot 2 Dry Blotting System (no ...
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bioRxiv - Plant Biology 2019Quote: ... 50 mg mL−1 Serva Blue G) and applied to the precast native 3-12% Bis-Tris polyacrylamide gel (Invitrogen, Life Technologies Corporation ...
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bioRxiv - Microbiology 2019Quote: ... The quality and quantity of the DNA was checked using gel electrophoresis and spectrophotometric analysis (NanoDrop 1000, Thermo Scientific, USA).