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Citations for Thermo Fisher :
7451 - 7500 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by SDS-PAGE and transferred on a PVDF membrane from pre-casted gels (Invitrogen, Carlsbad, CA, USA). Primary antibodies were diluted in 5% non-fat milk ...
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bioRxiv - Cell Biology 2020Quote: ... 20-25 μg of protein was separated on a NuPAGE 4–12% Bis-Tris gel (Life technologies, Carlsbad, CA, USA). Proteins were electroblotted to 0.45μm PVDF membranes (Imobilon-P ...
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bioRxiv - Cell Biology 2021Quote: ... and equal concentrations of each sample were separated on a 3-8% Tris-Acetate or 4-12% Bis-Tris gel (Invitrogen) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 minutes in NuPage LDS sample buffer and run on NuPage Bis-Tris 4-12% protein gels (Invitrogen), and proteins were transferred to PVDF membranes (Immobilon-FL ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR products were resolved by agarose gel electrophoresis and cloned in pCRTM4Blunt-TOPO® Vector (Invitrogen cat # K2875-20) by TA cloning ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were run in triplicate on a 4-12% Bis-Tris gel in 1X MES SDS running buffer for 40 minutes at 200V (all Invitrogen). Three biological replicates were run for each of the four groups ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA quality was checked on 1 agarose gels stained with RotiSafe (Roth) and a UV-VIS spectrometer (Nanodrop2000c, Thermo Scientific). DNA concentration was measured with Qubit fluorometer (BR dsDNA Assay Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were loaded in each lane and separated on a 4-12% Bis-Tris NuPAGE gel (Invitrogen), then transferred onto a PVDF membrane ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA quality was assessed by denaturing agarose gel electrophoresis and quantified by the Qubit RNA BR Assay Kit (ThermoFisher). For library preparation ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were loaded for each group in each well and run on 8-16% sodium dodecyl sulfate–polyacrylamide gel (ThermoFisher) for electrophoresis and then transferred to the PVDF membrane ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded onto a 10% gelatin gel (9-10 µL for apical, 18-20 µL for basal, Novex, Life Technologies). Samples were electrophoresed for ∼90 min at 125-140V ...
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bioRxiv - Cell Biology 2022Quote: ... Different concentrations of acrylamide and bis-acrylamide were mixed to produce gels of different rigidity 5 and mixed together with 2% v/v fluorescent carboxylated 200 nm beads (Invitrogen), 0.05% APS (A3678 ...
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bioRxiv - Neuroscience 2022Quote: ... The PCR products were run on a 2% agarose gel along with a 100 bp ladder (Thermo Fisher, Waltham, MA).
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bioRxiv - Immunology 2022Quote: ... 2.5 μg of GPC or GPC-I53-50A was mixed with loading dye and run on a 4%–12% Bis-Tris NuPAGE gel (Invitrogen). For SDS-PAGE analysis ...
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bioRxiv - Biophysics 2022Quote: ... 12% polyacrylamide-gel electrophoresis (PAGE) gels in 1x Tris Borate EDTA (TBE) running buffer and were visualized by staining with SYBR Gold II (Invitrogen) stain or fluorescence imager.
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bioRxiv - Cell Biology 2022Quote: ... Images of the stressed (before lysing) and unstressed (after lysing) gels were captured by the EVOS FL Auto cell imaging system (Invitrogen). An average of 10 cells was analysed per gel ...
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bioRxiv - Microbiology 2022Quote: ... Samples were loaded into two 15-well Novex™ WedgeWell™ 10% Tris-Glycine 1.0 mm Mini Protein Gels (Invitrogen), to a total protein amount of approximately 1.8 mg in a maximum volume of 30 µL ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of the His6 tag was assessed by running “pre-thrombin” and “post-thrombin” samples on a 4-12% BOLT SDS PAGE gel in MES buffer (Invitrogen); the difference in size between the His6-tagged and untagged version was clearly resolved on these gels ...
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bioRxiv - Biochemistry 2022Quote: ... purified PEP from chloroplasts was solubilized in Laemmli buffer and stacked in the top of a 4-12 % NuPAGE gel (Invitrogen). After staining with R-250 Coomassie Blue (Biorad) ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µg input protein (typically < 2% total input protein) and 40% of IP eluates were loaded onto 4-12% Bis-Tris gradient gels (Invitrogen) and analyzed by immunoblotting.
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bioRxiv - Biochemistry 2022Quote: ... Purity of the extracted 12×601 and 1×601 fragments were verified on 0.7% agarose gels and the concentration measured by 260 nm absorbance on a NanoDrop spectrophotometer (Thermo Scientific).
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bioRxiv - Biochemistry 2022Quote: ... 1:1 serial dilutions of UPF3B-WT were then added to the nucleotide solutions and incubated on ice for 30 mins before supplementing with 7.5% (final) glycerol and running on a Novex 6% Tris-Glycine WedgeWell gel (Invitrogen #XP0006A) equilibrated in Novex Tris-Glycine Native Running Buffer at 4 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA concentration was measured by gel electrophoresis (5µl sample on 1.2% agarose) and by spectrophotometry through Nanodrop 3300 (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 3 minutes at 95°C and loaded on NuPAGE 4 - 12 % Bis-Tris Mini Protein Gel (ThermoFisher Scientific). Proteins were separated by electrophoresis in NuPAGE MOPS SDS running buffer and transferred in Bjerrum Schafer-Nielsen transfer buffer (48 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated by size on a 12% sodium dodecyl sulfate polyacrylamide gel and blotted onto a 0.2 µm PVDF membrane (Thermo Scientific) by semi-dry blotting (BioRad) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples of elution fractions were taken to perform sodium dodecyl sulfate polyacrylamide agarose gel electrophoresis (SDS-PAGE) (Bolt 4-12% Bis-Tris Plus, Invitrogen) at 180 V for 30 min in 1× MES buffer (Novex ...
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bioRxiv - Pathology 2022Quote: ... for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher). After separation ...
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bioRxiv - Neuroscience 2022Quote: ... they were separated by 1D SDS/PAGE using Invitrogen NuPAGE 4-12% Bis-Tris midi gels (ThermoFisher Scientific, Massachusetts, USA) and Invitrogen MES running buffer using the manufacturer’s instructions (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were denatured at 75 °C for 20 min and run at 150 V for 1.5 h on 4-12% NuPAGE Bis-Tris gels in NuPAGE MOPS running buffer (Thermo Fisher). Proteins were transferred to polyvinylidene difluoride membrane using NuPAGE transfer buffer (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Protein purity was determined by resolving one to two micrograms of purified protein by SDS-PAGE using NuPAGE 4–12% Bis Tris precast gels (ThermoFisher) for 50 minutes at 200V ...
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bioRxiv - Microbiology 2022Quote: ... An equivalent of 0.25 midgut and 5 salivary glands were resolved in a NuPAGE™ 10% Bis-Tris Protein Gel (Invitrogen) under reducing conditions and transferred to a PVDF membrane Invitrogen™ Power Blotter Select Transfer Stacks ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated to 90°C for 10 min before being loaded on 4-12% Bis-Tris polyacrylamide gels (ThermoFisher). Each experiment was triplicated ...
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bioRxiv - Bioengineering 2022Quote: ... Cells within the HPL-based gels were than stained with AF® 488 phalloidin (0.1 U/ml, Molecular Probes, US) and HUVECs with a rabbit anti-human von Willebrand factor (vWF ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.06% Bromophenol Blue) at 98°C for 5 minutes and separated on 12% sodium dodecyl sulfate (SDS)-polyacrylamide gels (Invitrogen). Proteins were transferred to nitrocellulose membranes (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The integrity of all RNAs was verified by running RNAs on a MOPS-formaldehyde agarose gel and visualizing product by SybrGold staining (ThermoFisher). When required ...
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bioRxiv - Biophysics 2022Quote: SDS-PAGE electrophoresis was conducted by using Novex® NuPAGE® SDS-PAGE Gel System (Life Technologies, Carlsbad, CA, USA). NuPAGE® 4–12% Bis-Tris gels (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... a fraction of these (amount corresponding to approximately 0.5 pmoles of total ID2) were loaded on 4-12% Wedge-Well Tris- Glycine gels (Thermo Fisher). Following SDS-PAGE ...
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bioRxiv - Physiology 2022Quote: ... Lysates were separated by SDS–PAGE using the XCell SureLock Electrophoresis Cell on NuPAGE Novex 4%–12% Bis-Tris Protein Gels (Invitrogen). Proteins were transferred onto polyvinylidene fluoride membranes (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes using the iBlot2 Gel Transfer Device (Thermo Fisher Scientific). ALK (3633) ...
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bioRxiv - Immunology 2022Quote: ... Samples were then run on a 1.5% agarose (w/v) gel with a 100-base pair ladder (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... Proteins separated by their approximate sizes on the gel were then transferred onto nitro-cellular membrane (Thermo Fisher Scientific; 88018). We next blocked the membrane in blocking buffer (Li-Cor ...
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bioRxiv - Plant Biology 2021Quote: SDS-PAGE was performed using 14% (w/v) polyacrylamide gels according to (Laemmli, 1970) and the molecular weight markers Spectra Multicolor Broad Range Protein Ladder from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Samples were heated at 95 °C for 10 min before loading onto a 4-12% gradient Bis-Tris mini-gel (Invitrogen). After separation ...
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bioRxiv - Neuroscience 2021Quote: ... samples were embedded in a thin 4% polyacrylamide gel and then incubated for 48 hours at 37 ⁰C in digestion buffer (50 mM Tris, pH 8.0 (ThermoFisher 15568025), 1mM EDTA (ThermoFisher 15575020) ...
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bioRxiv - Neuroscience 2021Quote: ... Separation of an equal amount of proteins by SDS-PAGE was performed using 4-12% NuPAGE Tris-Acetate gels (Invitrogen). Subsequently ...
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bioRxiv - Microbiology 2019Quote: ... The quality of the DNA was assessed by gel electrophoresis and the quantity was estimated using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) and a Tecan Infinite 200 Microplate Reader (Tecan Deutschland GmbH).
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bioRxiv - Molecular Biology 2020Quote: ... Samples were boiled for 5min at 95°C before being loaded on a 3-8% NuPAGE Tris-Acetate gel (Invitrogen). Western blots were quantified using ImageJ ...
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bioRxiv - Plant Biology 2021Quote: ... The integrity of the RNA was verified on 1% agarose gel and quantified on the NanoDrop One spectrophotometer (Thermo Fisher). All samples used showed a ratio reading 1.8-2.0 of absorbance at 260/280 nm and 260/230 nm for high-quality RNA.
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bioRxiv - Microbiology 2021Quote: ... and was detected by the agarose gel electrophoresis as well as quantified by Qubit® 2.0 (Thermo Scientific, Waltham, USA). Libraries for ONT sequencing were prepared using an SQK-LSK109 kit of Oxford Nanopore Technologies Company ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were heated to 70°C and loaded on 3-8% Tris-Acetate Protein Gels (ThermoFisher Scientific, Waltham, MA) or NuPAGE 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific ...