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Citations for Thermo Fisher :
7401 - 7450 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 5–12 µl of protein lysate was loaded into pre-cast protein gels (4–12 % Bolt Bis Tris Plus, ThermoFisher) together with 10 µl of the molecular marker (PageRuler prestained ...
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bioRxiv - Microbiology 2020Quote: 1 μg of total RNA from mock- and ZIKV-infected cells was resuspended in 2x formamide RNA loading buffer and run on a 6% denaturing PAGE gel (Invitrogen) with an RNA ladder (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: Purified RNA of 10 μg was denatured at 95°C for 5 min in gel loading buffer II (#AM8547, Invitrogen) and separated by 6% polyacrylamide gel with 7M urea at 300 V for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... 0.1% v/v bromophenol blue in milli-Q water) and incubated at 95° C for 10 min prior to loading on a 4-12% Tris-Glycine gel (Invitrogen). For reducing SDS-PAGE ...
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bioRxiv - Systems Biology 2020Quote: ... 30 ul of the supernatant was loaded onto a NuPAGE™ 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific) and transferred using iBlot™ Transfer Stack to nitrocellulose (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein from the finished gel was transferred to a nitrocellulose membrane using an iBlot 2 Transfer stack (Invitrogen, Cat# IB23002). Membranes were washed in Odyssey Blocking buffer (LI-COR Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... crude extracts and purified recombinant SepF and FtsZ in serial dilution were loaded on a 4−12 % (w/v) BisTris PAGE gel (Invitrogen). The amounts of crude extract were 120 µg for FtsZ and 290 µg for SepF ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were size-selected (50-400 bp) using agarose gel electrophoresis and quantified fluorometrically using a Qubit 3.0 Fluorometer (Life technologies), and a total of 10 ng of digested DNA (200 pg l-1 ...
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bioRxiv - Neuroscience 2021Quote: ... cell lysates or precipitated media) were loaded on precast Nu-PAGE 4–12% Bis-Tris protein gels (Thermo Fisher Scientific), self-cast 10% or 12% SDS-gels or Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... Gels were run in 1X MOPS or 1X tris-acetate SDS running buffer containing 500 μL of antioxidant (Life technologies) for 90 min ...
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bioRxiv - Microbiology 2020Quote: Proteins were disrupted in NuPAGE loading buffer (Thermo) and separated by SDS-PAGE using 4-12% precast Tris-Glycine SDS polyacrylamide gels (Invitrogen) for 30min at 200V ...
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bioRxiv - Systems Biology 2020Quote: ... 20 μg of protein from each sample was separated on NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific) and transferred to nitrocellulose membranes (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... Protein lysates were separated by size on a 12% sodium dodecyl sulfatepolyacrylamid (SDS) gel and blotted onto a 0.2 µm polyvinylidene difluoride (PVDF) membrane (Thermo Scientific) by semi-dry blotting (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... loaded equivalently in each lane (ranging from 4-20 μg between experiments) and run on a 4-20% Tris-Glycine gel (Novex, Invitrogen) under reducing conditions ...
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bioRxiv - Microbiology 2021Quote: ... the extracted DNA samples were checked for quality and quantity by a 1% agarose gel (Midori Green-stained) electrophoresis and a Qubit dsDNA HS Assay Kit (Invitrogen). Prior to library preparation ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were submitted to an SDS-PAGE protein separation in 4-12 % or 3-8 % (depending on the molecular weight of the protein of interest) NuPAGE bis-tris gels (Invitrogen) and transferred to a nitrocellulose membrane (Amersham) ...
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bioRxiv - Neuroscience 2020Quote: ... 50 μg of proteins was separated by SDS-PAGE using 4–12% Novex Tris-Glycine gels in Novex Tris-Glycine SDS running buffer (all from ThermoFisher). Proteins were then transferred onto a polyvinylidene difluoride membrane (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... Amplification products are first visualized by electrophoresis in agarose gels and then purified by the ExoSAP-IT method using the Exonuclease-I and the Shrimp Alkaline Phosphatase (Invitrogen). The purified amplicons were sequenced using the Big Dye Terminators v3.1 kit (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... DNA-protein complexes on the gel were transferred to 0.45mm Biodyne™ Pre-Cut Modified Nylon Membrane (77016, Thermo Scientific) at 380 mÅ for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Protein purity was determined by resolving one to two micrograms of purified protein by SDS-PAGE using NuPAGE 4–12 % Bis Tris precast gels (ThermoFisher) for 50 minutes at 200 V ...
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bioRxiv - Microbiology 2020Quote: ... and RNA integrity was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... all the enzymes were buffer exchanged against 1 mol·L−1 ammonium acetate at pH 7.5 using microcentrifuge gel-filtration columns (Zeba 0.5 mL, Thermo Scientific, Rockford, IL). Protein concentration was determined with a NanoDrop™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Proteins present in a 2 µL aliquot of each preparation were separated on a 10% SDS gel containing 0.1% gelatin (ThermoFisher, #ZY00105BOX) under non-reducing ...
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bioRxiv - Systems Biology 2020Quote: ... Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific) and transferred to PVDF membrane using an iBlot™ 2 dry blotting system (Invitrogen/Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... 5-10 μl of boiled supernatant of whole cell lysates was resolved on 4-12% Bis-Tris NuPAGE protein gel (Invitrogen) in 1×MES buffer (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... in non-reducing conditions and the proteins were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Gel, Life Technologies). Proteins were transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Briefly,10μg of denatured protein was loaded onto Novex 4-20% Tris-Glycine Mini Gels (Thermo Fisher Scientific, MA, USA) and subjected to electrophoresis (45 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... were removed from the glass plates and incubated in ∼140 ml of TBE + 14 μl SYBR Gold Nucleic Acid Gel Stain (Invitrogen) for several minutes then destained in TBE for ∼10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 µg of total protein were loaded per well on Bolt™ 4–12 % Bis-Tris gels (Thermo Scientific, #NW04120BOX) and blotted on a 0.2 µm PVDF membrane (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were separated by electrophoresis on a 1.5% agarose gel and stained with SYBR® safe (Thermo Fisher Scientific) before being visualized under an ultraviolet transilluminator ...
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bioRxiv - Microbiology 2020Quote: Proteins were mixed with NuPAGE reducing sample buffer and boiled at 70°C for 10 min and separated by SDS-PAGE on NuPAGE 4–12% Bis-Tris gels in anti-oxidant buffer (all from ThermoFisher). Resolved proteins were transferred to a nitrocellulose membrane using the Trans-blot Turbo system (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... 100μg of whole cell lysate from each strain was run on NuPage 4-12% bis-tris protein gel (Thermo Scientific) at 120V for 3hours ...
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bioRxiv - Neuroscience 2021Quote: ... 20-25 µg of RIPA or SDS lysate or 1 µg of S4 lysate were separated in a 4-20% Tris-glycine gel (Novex, Invitrogen) and transferred to PVDF membranes ...
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bioRxiv - Genomics 2021Quote: ... The quality and quantity of the genomic DNA were assessed on Agarose gel and Nano-drop One (Thermo Fisher Scientific). The paired-end (PE ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA fragments resulting from nuclease activity during the Surveyor assay were separated by electrophoresis in 10% TBE-polyacrylamide gels (ThermoFisher). A quantitative measurement of optical density through Image J was performed to quantify efficiency of CRISPR/Cas9 editing ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were analyzed by 1% agarose gel electrophoresis and visualized under UV illumination after SYBR-Safe staining (Invitrogen, S33102). For Sanger sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Western blotting was completed using 4-12% Bis-Tris pre-cast gels and MOPS running buffer purchased from Invitrogen (USA), Nitrocellulose membrane obtained from GE Healthcare (Amersham ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples (50µg total protein) were resolved by SDS-PAGE using 4-12% Bis-Tris gel (Nupage, Invitrogen, Carlsbad, CA) and transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... protein samples were separated using pre-cast 4%-12% Bis-Tris Gels (Thermo-Fisher) and stained using the Silver Quest Staining Kit (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins were briefly subjected to SDS-PAGE (3 min at 200 V, 4– 12% Bis-Tris gel, Life Technologies) and stained with InstantBlue Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... so that 50 μg of total protein could be mixed with 5% Coomassie brilliant blue G-250 before loading on a 3-12% Bis-Tris BNE gel (Invitrogen). After electrophoresis (2.5 hr ...
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bioRxiv - Cell Biology 2021Quote: ... Purified p roteins (35μl) were run on a 10% SDS-PAGE gel (1mm) and stained with colloidal Coomassie Brilliant Blue (CBB, Novex, Invitrogen). Those with a weight >28 kD (Streptavidin dimer ...
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bioRxiv - Cell Biology 2020Quote: ... The gel was rinsed with PBS then covered with 1% UltraPure™ Low Melting Point Agarose (16520050 Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated for 2 min at 90 °C and 18 μL of resuspended cross-linked pellets were loaded onto a 4-12% pre-cast Novex Tris-Glycine gels (Invitrogen). Further immunodetection of caspase-4 was performed following standard western blotting procedures.
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bioRxiv - Cell Biology 2020Quote: ... all the crosslinking samples were boiled for 10 min and resolved on a NuPAGE 4–12% gradient polyacrylamide gel (Invitrogen). Gels were stained using SimplyBlue (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were resolved by SDS-PAGE using Novex NuPAGE pre-cast gradient Bis-Tris acrylamide gels (4-12%) (Invitrogen). After gel electrophoresis ...
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bioRxiv - Biophysics 2020Quote: ... Samples were run on a 16% tricine gel and stained using a Peirce Silver Stain Kit (Thermo Scientific, Waltham MA). Bands were quantified in ImageJ using the Band Peak Quantification plugin.
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bioRxiv - Biophysics 2020Quote: ... The samples were incubated for 20 min at 4°C before they were loaded onto a pre-run 4-20% Novex TBE gel (Invitrogen). Electrophoresis was performed at 200 V for 45 min in TBE buffer (89 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... and equal amounts of protein (20-40 μg) were run on NuPage 4-12% Bis-Tris gradient gels (Thermo Fisher) by SDS-PAGE and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: Denatured protein lysates (15 μg/sample) were loaded on to NuPAGE 4-12% Bis-Tris 1.0 mm Mini Protein Gels (Invitrogen, NP0321) alongside Chameleon Duo Protein Ladder (3 μL/lane ...