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Citations for Thermo Fisher :
7351 - 7400 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... Proteins were loaded on 4-20%Tris-Gly gradient gels (invitrogen XP04200BOX) then transferred to nitrocellulose membranes with a dry transfer system (Invitrogen iBIot Blotting System and Gel Transfer Stacks Invitrogen IB301001) ...
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bioRxiv - Microbiology 2019Quote: ... These gels were dried down and visualized using a phosphor storage screen (imaged with a GE Life Technologies Typhoon 9500). Reactions containing large substrates were resolved on 6% polyacrylamide (37.5:1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Thirty μg of protein in each sample was resolved by electrophoresis using 4-12% bis-tris gels (Thermo Fisher Scientific) and transferred to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... The samples were heated at 95°C for 10 minutes and centrifuged at 4°C for 10 minutes to remove potential protein aggregates before proteins were separated in gradient polyacrylamide gels (4-12%) (Invitrogen). Proteins were transferred onto nitrocellulose membranes (Whatman ...
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bioRxiv - Cancer Biology 2019Quote: ... and samples were denatured at 70C for 10 minutes in NuPAGE LDS sample buffer with NuPAGE sample reducing agent and then resolved on a 4-12% Bis-Tris gradient gel (Invitrogen). For chemiluminescent detection ...
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bioRxiv - Physiology 2019Quote: ... 20-30 μ g of protein lysates were denatured in NuPAGE 4× LDS sample buffer and resolved on NuPage 4-12 % Bis-Tris gels (Invitrogen) and the proteins transferred by iBlot (Invitrogen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 3 μl of PCR product was visualised on a 1.4% agarose gel and then purified with FastAP and Exonuclease I (Thermo Scientific). 1.0–2.0 U of both enzymes were added to 12–27 μl of PCR solution and incubated for 15 min at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... Cell lysates dissolved in SDS load buffer were heated at 90°C for 3 min and separated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to a PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Genetics 2019Quote: ... by incubating at 70°C for 10 minutes and separated on 4-12% NuPAGE Bis-Tris gels (Thermo Fisher Scientific) along with 5% of input samples and analysed by Western blotting as described above.
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bioRxiv - Cancer Biology 2020Quote: ... ATM and ATR in the CE samples as follows: the samples were loaded on 4-8% (w/v) Tris-acetate SDS-PAGE gels (ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: Total protein from equal amounts of cells was separated on a NuPage Gradient 4-12% Bis-Tris Mini Gel (Invitrogen), transferred to a PVDF membrane ...
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bioRxiv - Neuroscience 2020Quote: ... Undigested bands were gel extracted and subcloned into pJet2-1 using the Clone JET PCR Cloning kit (Thermo Fisher Scientific) and sequence-verified.
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bioRxiv - Neuroscience 2021Quote: ... 25 µl of supernatant was mixed with loading buffer and resolved by SDS-PAGE in 4- 12% Bis–Tris gels (Invitrogen) under reducing conditions and electro-blotted onto nitrocellulose membranes ...
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 4-12% Novex WedgeWell Tris-Glycine gels (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 12% Novex WedgeWell Tris-Glycine gels (ThermoFisher). Protein bands were stained with an InstantBlue Coomassie solution (Abcam ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Approximately 20 μg of protein per sample was loaded into a NativePAGE Novex 4-16% Bis-Tris polyacrylamide gel (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were visualized by electrophoresis on a 1% agarose and 1X TAE gel containing 0.3 μg/mL ethidium bromide (Invitrogen, 15585011). Gel extraction was performed to select for products between 200 – 1000 bp using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel ...
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bioRxiv - Biophysics 2021Quote: Purity of protein constructs was checked by running them on a 12% SDS-PAGE gel followed by staining with Coomassie blue (SimplyBlueStain, Invitrogen) and imaging on a ChemiDoc MP (BioRad).
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bioRxiv - Cell Biology 2020Quote: ... Laemmli sample buffer (0.1 mL of 4x) was added to lysate prior to loading 10 μL on a 4%–12% Bis-Tris NuPAGE gel (Invitrogen). Gels were transferred onto a Mini-size polyvinylidene difluoride membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... WCE and bound proteins were separated by running in a 4-12% Bis-Tris gradient gel with MOPS SDS running buffer (Invitrogen). Mif2-TAF and Flag-Cse4 were detected by immunoblotting with anti-protein A (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples (10-20 μg) were run on SDS/PAGE with NuPAGE precast 4-12% gradient gels (Invitrogen; Carlsbad, CA, USA) and blots were incubated overnight at 4°C with primary antibodies (Supplementary Table 1 ...
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bioRxiv - Bioengineering 2021Quote: ... 10 µg of each sample was boiled at 95°C for 5 min and loaded onto the 4-12% Bis-Tris Gel (NP0336, ThermoFisher). Gels were run for 1 hour and 15 min at 120 V in with NuPage MES Buffer (NP0002 ...
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bioRxiv - Cell Biology 2021Quote: ... The obtained protein samples were separated on 4-12% Bis-TRIS NuPAGE gel in 1 × MES buffer (Thermo Fisher Scientific) and transfer to nitrocellulose membrane before blocking with 5% non-fat milk dissolved in 1 × TRIS-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 mg Orange G in 10 mL H2O) was added to each reaction and run on an 8% TBE gel (Invitrogen) (180 V ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates containing 10 μg of total protein were loaded (without boiling) on precast NuPAGE 4-12% Bis-Tris gels (Invitrogen) for SDS-PAGE analysis ...
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bioRxiv - Neuroscience 2020Quote: ... Brain extracts containing 25 μg of total protein were loaded (without boiling) on precast NuPAGE 4-12% Bis-Tris gels (Invitrogen) for SDS-PAGE analysis ...
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bioRxiv - Bioengineering 2020Quote: Equal masses of protein lysate or equal volumes of immunoprecipitation eluents were loaded into NuPAGE 4-12% Bis-Tris gels (ThermoFisher). After electrophoresis ...
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bioRxiv - Bioengineering 2020Quote: ... For SDS-PAGE analysis proteins were run under reducing and non-reducing conditions on 10 or 12% acrylamide gels (Invitrogen) and stained using Coomassie blue ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 10 minutes at 70°C and then loaded into a 4-12% NuPAGE Bis-Tris gel (Invitrogen, NP0326BOX). After electrophoresis ...
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bioRxiv - Biochemistry 2021Quote: ... and samples were incubated at 70°C for 5 minutes and loaded onto Bis-Tris 4-12 % gradient gels (ThermoFisher). Bands were stained by CBB and relative band intensity was quantified by scanning and semi-automated analysis in ImageQuant (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... Reaction products were then analysed by running on a 20% non-denaturing PAGE gel in 0.5X TBE buffer (Life Technologies) for 20 min at room temperature and 200 V ...
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bioRxiv - Molecular Biology 2021Quote: The purified DNA fragments separated by electrophoresis in 1% agarose gel and transferred by the alkaline capillarity method to the Bio-dyne nylon membrane (Invitrogen) in a denaturing solution (NaOH 0.4 M) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resolved on NuPage 3-8% w/v Tris-Acetate or 4-12% w/v Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2020Quote: ... The isolated fractions were separated on SDS-PAGE (RunBlue 4-20 %, Expedeon; NuPAGE®Bis-Tris gel 4-12%, Invitrogen) and analysed by InstantBlue staining (Expedeon ...
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bioRxiv - Neuroscience 2020Quote: ... 10-30 μg of samples were loaded onto NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1403BOX) and electrophoresed at 130 V for 2 h with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were subjected to electrophoretic separation on a 4-20% Tris-Glycine polyacrylamide gel (ThermoFisher Scientific, #XP04200), transferred to Nitrocellulose membrane (LI-COR ...
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bioRxiv - Microbiology 2021Quote: Proteins were separated by electrophoresis using a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and transferred to a Hybond-P PVDF membrane (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: 20 µg of proteins extracted in SDS buffer (1% SDS supplemented with protease and phosphatase inhibitors) were loaded on to a 4-12% gradient SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 40μg of protein were added to each lane and samples were electrophoresed on 4-12% Bis-Tris gels (NuPage, Thermofisher). Gels were then transferred to PVDF membrane ...
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bioRxiv - Cell Biology 2021Quote: ... and one-fifth of the affinity purification (IP) were separated on Bolt 4-12% Bis-Tris Plus 15-well gels (Invitrogen), run in Bolt 1x MOPS SDS running buffer (Novex ...
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bioRxiv - Cell Biology 2021Quote: ... the total amount of PCR product was separated by electrophoresis at 110 V for 40 minutes in 1.8% agarose gel diluted in 1 × Tris-acetate EDTA buffer (w/v) and stained with 0.01% of SYBR Safe (Thermo Fisher).
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were transferred to a nitrocellulose membrane (iBlot Gel Transfer Stacks Nitrocellulose) using an iBlot dry blotting system (Life Technologies). After blocking ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were run on 2% agarose gels using ethidium bromide staining along with the 100-bp DNA ladder (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Extracts were analyzed by SDS–PAGE using a 4-12% Bis–Tris or 3-8% Tris-Acetate NuPAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: A total of 50 μg of protein for each of the samples was loaded into a pre-cast NativePAGE 3-12% Bis-Tris gel (Invitrogen). For the run ...
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bioRxiv - Physiology 2021Quote: ... Specific amounts of protein were separated by SDS–PAGE with precast Bolt 4-12% Bis-Tris gel (Thermo Fisher Scientific) or Mini PROTEAN TGX precast 4-20% (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... DNA quality was checked by agarose gel electrophoresis and quantified with a Qubit® fluorometer (Thermo Fisher Scientific, Waltham, MA). Library construction and sequencing was based on the 96-plex genotyping-by-sequencing (GBS ...
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bioRxiv - Plant Biology 2021Quote: ... for one hour followed by alkylation with 55 mM chloroacetamide (CAA) for 30min at room temperature and run into a 4-12% NuPAGE gel (Invitrogen) for approximately 1 cm ...
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bioRxiv - Cell Biology 2021Quote: ... Specific amounts of protein were separated by SDS–PAGE with precast Bolt 4-12% Bis-Tris gel (Thermo Fisher Scientific) or Mini PROTEAN TGX precast 7,5% gel (Bio-Rad) ...