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Citations for Thermo Fisher :
7251 - 7300 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Gels were then stained with the ultra-sensitive Pierce Silver Stain Kit (Thermo Fisher Scientific™, catalog no. 24612) following the manufactureŕs instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was incubated for 15 minutes at room temperature and then separated on a 6% DNA retardation gel in 0.5X TBE running buffer (ThermoFisher). The gel was imaged with a SapphireTM multimode imager in the Keck Biophysics facility at Northwestern University.
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bioRxiv - Cell Biology 2024Quote: ... and 20μg of proteins were electrophoresed on SDS–PAGE gels and then transferred to PVDF membrane (Thermo Scientific, # 88518). The membranes were blocked using 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... PAA gels were washed gently once with PBS followed by coating with 100 μg/ml collagen type I (Gibco) in PBS overnight at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... and BN-PAGE gels were run for 2-2.5 h at 150 V using NativePAGE running buffer (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation and were resolved with NuPAGE 4-12% Bis-Tris protein gel (Thermo Fisher Scientific) prior to transfer onto nitrocellulose membranes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... For visualization of electrophoretic gels we used SYBR® Safe DNA binding dye (Invitrogen Life Technologies, Carlsbad CA, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... For visualization of electrophoretic gels we used SYBR® Safe DNA binding dye (Invitrogen Life Technologies, Carlsbad CA, USA).
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bioRxiv - Bioengineering 2024Quote: ... SYBR Green I nucleic acid gel stain (10,000X concentrate in DMSO) (S7563) and Qubit dsDNA HS Assay Kit (Q32851) were purchased from Invitrogen. UltraPure TBE buffer (10X) ...
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bioRxiv - Plant Biology 2024Quote: ... 50 μg of sample was then loaded onto an SDS PAGE pre-cast 4-12% Bis Tris gel (ThermoFisher) and separated with MOPS buffer (ThermoFisher) ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: ... 2/3rd of the eluted protein samples were resolved on a 4–12% Bris-Tris gel (Invitrogen: cat# NW04125box) and subjected to Silverstein (Pierce™ Silver Stain Kit ...
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bioRxiv - Bioengineering 2024Quote: Protein samples were diluted in PBS to 5 μM and mixed with 5000× SYPRO Orange Protein Gel Stain (Invitrogen) to final 1.25x concentration ...
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bioRxiv - Immunology 2024Quote: Protein lysates were separated using 4-12% Bis-Tris SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Thermo Fisher Scientific, #NP0321) for 1-2 h at 150V ...
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bioRxiv - Microbiology 2024Quote: ... The quality of the extracted DNA was measured by 1% agarose gel electrophoresis and Qubit® 3.0 Fluorometer (ThermoFisher). Subsequently ...
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bioRxiv - Synthetic Biology 2024Quote: ... The samples were first separated by 12% SDS-PAGE gel and then transferred to a nitrocellulose membrane (Fisher Scientific). The membranes were incubated with the Anti-FLAG epitope (DYKDDDDK ...
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bioRxiv - Microbiology 2024Quote: ... The LOS extracts were then run on NuPAGE Bis-Tris gradient (4-12%) SDS-PAGE gels (Invitrogen, Waltham, USA) with NuPAGE MES running buffer (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... heated to 70°C for 5 min and ran on a 4-12% Nupage precast gel (Thermo Fisher Scientific). Protein were then detected using InstantBlue™ Coomassie Stain (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... HEPA1-6 were lysed in RIPA buffer and loaded on a 4-15% gradient polyacrylamide gel (Thermo Fisher, NP0335). Protein transfer was carried out using the iBlot system (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... Different concentrations of acrylamide and bis-acrylamide were mixed to produce gels of different rigidity53 and mixed with 0.04% v/v fluorescent carboxylated 200 nm beads (Invitrogen), 0.05% APS (Sigma-Aldrich A3678) ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 µg protein were loaded onto 4-12% Bis-Tris polyacrylamide gel (Thermo Fisher Scientific; Cat No. NP0336BOX) Nitrocellulose (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... Time points were resolved by SDS-PAGE on NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gels (Invitrogen) in MOPS running buffer ...
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bioRxiv - Plant Biology 2020Quote: ... We controlled the RNA integrity through agarose gel electrophoresis (1.5 %) and RNA concentration and purity were measured with a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2020Quote: ... and 7.5 µg of lysate was run on a 4-12% Bis-Tris sodium dodecyl sulfate (SDS)-polyacrylamide gel with MOPS running buffer (Invitrogen) under reducing conditions ...
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bioRxiv - Biochemistry 2020Quote: ... Denatured protein samples were then resolved on a 10% polyacrylamide gel and transferred to a PVDF membrane (Thermo Scientific, 88518). Blots were probed as previously described 37 ...
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bioRxiv - Neuroscience 2021Quote: ... and 20 µg protein lysate was denatured at 95°C for 5 min and then separated on a 10-20% Tris-tricine gel (Invitrogen) at 125V for 90 min ...
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bioRxiv - Biophysics 2019Quote: ... the sample was heated to 95 °C for 5 min and 15 µl of sample were resolved by a 12% NuPAGE Bis-Tris gel (Invitrogen). Proteins were transferred onto a Immobilon-P polyvinylidene difluoride membrane (Millipore ...
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bioRxiv - Biophysics 2019Quote: ... the sample was heated to 95 °C for 5 min and 15 µl of the sample were resolved by 12% NuPAGE Bis-Tris gel (Invitrogen). Molecular weight markers were purchased from ThermoFisher Scientific (PageRuler™ Plus Prestained Protein Ladder ...
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bioRxiv - Cancer Biology 2021Quote: ... 4X SDS-loading buffer was added to protein lysates and 15 μg of lysate were resolved on a Bolt 4-12% gel (NW04120BOX, Invitrogen). Gels were transferred onto an Immobilon-P membrane (IPVH00010 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was quantified with BCA (Pierce) and samples were loaded onto 4-12% Bis-Tris SDS-PAGE gels (Invitrogen) and electrophoresed at 150V until the loading dye reached the bottom of the gel ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell extracts (20 µg/lane) were fractionated in 3%-8% or 4-12 % tris-acetate gel (NP0006, NuPAGE Life Technologies). All antibodies were used at the concentrations recommended by the manufacturers.
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bioRxiv - Cancer Biology 2021Quote: ... equal protein amounts were loaded and then separated by electrophoresis through NuPAGE Novex Bis-Tris 4–12% gradient gels (Invitrogen). Proteins were transferred onto Nitrocellulose membranes using the iBlot system and protocol from InVitrogen (Carlsbad ...
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bioRxiv - Systems Biology 2020Quote: ... Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific) and transferred to PVDF membrane using an iBlot™ 2 dry blotting system (Invitrogen/Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were migrated on a SDS-PAGE gel (12% acrylamide) and transferred to Amersham Hybond P 0.2 PVDF membranes using a Pierce G2 Fast Blotter (Thermo Scientific). Membranes were blocked in 5% w/v milk or BSA ...
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bioRxiv - Microbiology 2021Quote: ... We mixed an equal volume of the extracted LPS samples with an equal volume of 2× SDS buffer and electrophoresed 10 µl of each sample on Novex 4-20% plyacrylamide gradient gels (ThermoFisher) in Tris-Glycine-SDS buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 0.2% (v/v) Triton X-100) and analyzed by SDS-PAGE on 4-12% NuPAGE Bis-Tris gradient gels (Invitrogen) followed by immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then eluted with Laemmli buffer and processed for SDS-PAGE in Novex Value 4-20% Tris-Glycine gels (Thermofisher). Proteins were then transferred to nitrocellulose or PVDF membranes and analyzed by immunoblot ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then eluted with Laemmli buffer and processed for SDS-PAGE in Novex Value 4-20% Tris-Glycine gels (Thermofisher). Proteins were then transferred to nitrocellulose or PVDF membranes and analyzed by immunoblot ...
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bioRxiv - Cell Biology 2020Quote: ... The eluted proteins were separated on a 4-12% NuPAGE gel (Novex) and stained with Imperial protein stain (Thermo Scientific). The proteins of interest were excised for identification by MS.
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bioRxiv - Cell Biology 2020Quote: ... IP and lysate samples were separated by SDS-PAGE on precast 3-8% tris-acetate and 4-12% bis-tris acrylamide gels (Invitrogen) followed by transfer to 0.2 μm nitrocellulose membranes (Amersham) ...
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bioRxiv - Cell Biology 2020Quote: ... was used to extract protein from mOSE cells and run on NuPAGE 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Cell Biology 2020Quote: ... Proteins were resolved by standard SDS polyacrylamide gel electrophoresis using pre-stained PAGE RULER plus (Thermo Fisher Scientific, cat# 26619) molecular weight markers ...
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bioRxiv - Immunology 2021Quote: ... in an SDS running buffer (192 mM glycine, 25 mM Tris base, 0.1% SDS) or using NuPAGETM 4-12% gradient gels (Invitrogen WG1403BX10) in NuPAGETM MOPS-SDS running buffer (Invitrogen NP0001-02) ...
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bioRxiv - Neuroscience 2020Quote: ... or 30 μg (retinal extract) of proteins were loaded per lane on NuPAGE 4-12% (wt/vol) Bis-Tris gels (Invitrogen). Proteins were transferred onto a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... Blots were imaged on a SynGene gel imaging system after a 1min exposure to SuperSignal Femto West Maximum Sensitivity Substrate (ThermoFisher), and band density was quantified using GeneTools software ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplified fragments were resolved in agarose 0.8% (w/v) gel electrophoresis together with 1 Kb Plus Ladder (Invitrogen, 10787026). While vsp1 ...
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bioRxiv - Microbiology 2021Quote: ... and 30 μl of each sample was used to load duplicate NuPAGE 4-12% Bis-Tris acrylamide gels (Invitrogen, #NP0321) which were electrophoresed simultaneously ...
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bioRxiv - Biophysics 2021Quote: ... was added to the samples and pellet fractions were run on a 4-12% BIS-Tris gel (Thermo Fisher Scientific). The gel was stained with EZBlue reagent (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: SDS-PAGE was performed using NuPAGE Novex 4-12% Bis-Tris gels according to the instructions of the manufacturer (Invitrogen). Gels were stained with SimplyBlue safe stain (Life technologies) ...
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bioRxiv - Immunology 2021Quote: Pneumococcal strains were mixed with non-reducing loading buffer (Laemmli SDS sample buffer, non-reducing 6X) and loaded on a 4-12% Bis-Tris gel (Invitrogen). Samples were then transferred to PVDF membranes via iBlot system (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Surveyor assay was performed using Sarm1AG3S PCR conditions and Surveyor Mutation Detection Kit (IDT) followed by electrophoresis on Novex 20% TBE gels (Invitrogen). Off-target CRISPR cleavage was accessed by PCR amplification using the primers listed in table I and cycling conditions 95° 30 sec ...