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Citations for Thermo Fisher :
7201 - 7250 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Proteins in cell lysates (20-40 μg) were separated on precast NuPAGE™ 4-12% Bis-Tris gels (Invitrogen) using MES (Figure 4 ...
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bioRxiv - Biophysics 2023Quote: ... Total cell lysates were prepared and analyzed by SDS-PAGE using 4-12% gradient Bis-Tris NuPAGE gels (ThermoFisher) followed by immunoblotting71 ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg of protein were resolved using a NuPAGE (4– 12%) Bis-Tris gel (Thermo-Fisher Scientific, Cat. # NP0322PK2). After the run ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... Equal amounts of protein were loaded and resolved on a 4–12% Bis-Tris gradient gel (Thermo Fisher Scientific) and analyzed by western blot with anti-SARS-CoV-2 N protein monoclonal antibody (0.5 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Equal amounts of protein samples within each group were loaded and resolved using 4–12% NuPAGE gels (Life Technologies), and subsequently electro-transferred onto nitrocellulose membrane (iBlot ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were prepared and run on NativePage 4-12% Bis-Tris gels as per the manufacturer’s instructions (Life Technologies). For immunoblotting ...
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bioRxiv - Molecular Biology 2023Quote: ... The sample was eluted with 20 µl Laemmli buffer and further analyzed by immunoblotting using anti- IFITM3 (1:2,000) and anti-Caveolin1 (1:1,000) antibodies on a pre-cast 10 – 20% Tricine gel (ThermoFisher Scientific). The ‘Precision Plus’ (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Gels were run at 150 V for 2h in 1× NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific). Protein was transferred onto a PVDF membrane (Merck ...
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bioRxiv - Cell Biology 2023Quote: Lysates containing 25 µg of protein were electrophoresed on a NuPage 4%-12% Bis-Tris Protein gel (Thermofisher Scientific). Proteins were transferred to a PVDF membrane (0.2 µm ...
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bioRxiv - Biophysics 2023Quote: ... fluorescent dye were added to gel filtration buffer containing 0.2 mg/mL (final concentration) Cascade Blue Hydrazide (ThermoFisher Scientific) and either 1 μM WT NEC or NEC mutant (final concentration) ...
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bioRxiv - Biochemistry 2023Quote: ... Matrix Sciences) and the results from each gel slice compiled into a consensus report using Proteome Discoverer (Thermo Scientific). The MS data were compared with the Uniprot sequence database (August 2020 version containing 563,000 sequences ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were solubilized in Laemmli buffer and stacked in the top of a 4-12% NuPAGE gel (Invitrogen). After staining with R-250 Coomassie Blue (Biorad) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were visualized in 1% agarose gel stained with SYBR safe (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 10 min at 95C and subjected to NuPage 3-8% Tris-Acetate gel (#EA0375PK2, Invitrogen). Separated proteins were transferred to cellulose membrane using a semi-dry transfer method and blocked in TBS-T with 5% dry milk containing 2% normal goat serum for 1hr ...
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bioRxiv - Bioengineering 2023Quote: ... We activate the gel surface by sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino) (sulfo-SANPAH, Thermo Scientific, Cat. No. 22589), which allows covalent conjugation of type IV collagen to the gel surface ...
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bioRxiv - Cancer Biology 2023Quote: ... aliquots from each sample containing 50 µg of protein were separated on Novex WedgeWell 10% Tris-Glycine gel (Invitrogen) and transferred to nitrocellulose membrane with iBlot 2 Dry Blotting System (Invitrogen).The blots were subsequently blocked for 1 h with 5% milk powder in Tris-buffered saline (TBS)/Tween 20 at RT and then probed overnight (at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: ... then ran 20 µg total protein on a NuPAGE 4-10% BisTris Gel at 240V for 40 minutes (ThermoFisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... Samples were denatured for 2 minutes at 90°C and hemolymph from 10 mosquitoes was loaded on each lane of a 4 - 20% 1.5mm polyacrylamide gel (Invitrogen). After electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... the products were purified following manufacturer’s protocols by resolving on a 1 % agarose gel with SYBR Safe (ThermoFisher Scientific), followed by a gel extraction (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were separated using precast 4–20% Tris-Glycine SDS-PAGE gels (1.0 mm thick) (Life Technologies, Carlsbad, CA). Protein gel was stained with Simply Blue SafeStain (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Equivalent protein quantities for all genotypes were loaded onto a Nupage 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Protein purity and size were checked trough SDS-Page on Novex WedgeWell 12% Tris-Glycine Gel (Invitrogen, Waltham, US). The protein ladder used was Colour Protein Standard Broad Range (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 μg of protein were loaded and run on pre-casted 4-12% NuPAGE Bis-Tris gels (Invitrogen #NP0335) for 90 minutes at 120V ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were run in a 3-8% Tris-acetate gel with 1X NuPAGE MES Running buffer (Life Technologies, Invitrogen). A wet transfer was done overnight at 30 V ...
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bioRxiv - Microbiology 2023Quote: ... After staining the gel for 10 min in 1x TBE buffer with a 1:10,000 dilution of SYBR Gold Nucleic Acid Gel Stain (Invitrogen), the bands in the range of 130- 1000 bp were cut into small pieces and transfer to a 2 ml LoBind tube ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were run in a 3-8% Tris-acetate gel with 1X NuPAGE MES Running buffer (Life Technologies, Invitrogen). A wet transfer was done overnight at 30 V ...
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bioRxiv - Molecular Biology 2023Quote: ... Purification of PCR products was performed using the Gene JET™ Gel Extraction Kit (Cat#: K069, Thermo Scientific, China). Sequencing library was constructed using Ion Plus Fragment Library Kit 48 rxns (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Physiology 2023Quote: ... The samples were run under reducing conditions with 4-12% Bolt™ Bis-Tris Plus pre-cast gels (Invitrogen) and proteins were transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were prepared via PCR as previously described55 and purified via GeneJET Gel Extraction Kit (Thermo Fisher Scientific).
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bioRxiv - Genetics 2023Quote: RNA concentration and quality were determined by gel electrophoresis in 1% UltraPure™ agarose (Invitrogen Corp. Carlsbad, California, USA) in 1× TBE running buffer at 100 V for 40 min ...
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bioRxiv - Immunology 2023Quote: Proteins in VLP containing samples were separated by SDS-PAGE using 4-12% Bis-Tris Nu-PAGE gels (Invitrogen) and electro-transferred to a PVDF membrane using the Trans-Blot Turbo Transfer Pack (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... Amplification of PCR products for each sample was confirmed by both gel electrophoresis and Qubit Fluorometer (Invitrogen, Carlsbad, CA). We processed PCR blanks (molecular grade water ...
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bioRxiv - Genomics 2023Quote: ... the eluate was added to 2 μL of 1X E-Gel Sample Loading Buffer (Thermo Fisher Scientific, cat # 10482055) and the resulting 20 μL loaded onto a well on a 2% Agarose E-gel (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 2 ug of EV protein content were separated on a 4-12% Bis-Tris gel (Thermo Scientific, Rockford, IL), then blotted onto a PVDF membrane ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was then electrophoresed on a 3-12% Native PAGE Novex Bis-Tris gel (Invitrogen, Inc. Waltham, MA) at 80 V for 4 hours at 4°C in the supplier’s buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... An equal amount of total protein was resolved using SDS-PAGE gels and transferred to nitrocellulose membranes (Fisher Scientific) at 110mA for 2h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 8 µg of protein was separated on a NuPAGE 4–12% Bis-Tris gel (Life technologies, Carlsbad, CA, USA). Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P ...
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bioRxiv - Biochemistry 2023Quote: ... All samples were loaded on pre-casted SDS Tris-glycine 4–20% polyacrylamide gels (Life Technologies, Carlsbad, CA, USA). For western blot detection ...
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bioRxiv - Biochemistry 2023Quote: ... in-gel histone acylation using propionic anhydride and digestion with trypsin [MS Grade Pierce™ Trypsin Protease (Thermo Scientific)] were performed ...
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bioRxiv - Cell Biology 2023Quote: ... Electrophoresis was performed with 10 μL of sample per well using the Novex NuPAGE SDS-PAGE Gel System (Invitrogen). Proteins were transferred from the gel onto PVDF membranes (EMD Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Linearization efficiency was qualitatively determined using 1.2% agarose gel electrophoresis and DNA concentration was quantitatively determined using NanodropTM 8000 spectrophotometer (ThermoFisher). To investigate if DNA linearized by BspQI@BG can be used directly in IVT without purification ...
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bioRxiv - Biophysics 2023Quote: ... then the pooled amplicon libraries were gel extracted and quantified using the QuantIt dsDNA assay kit (Invitrogen ref Q33130) or via qPCR at the Vincent J ...
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bioRxiv - Biochemistry 2023Quote: ... Handcast SDS-PAGE gels (8%, 10%, 15%, or 4-18% gradient) containing 0.5% (v/v) trichloroethanol (Acros Organics 139441000) for stain-free imaging (79 ...
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bioRxiv - Genetics 2023Quote: ... followed by DNA quantification and quality assessment on agarose gels and using the Quant-It PicoGreen assay (Thermofisher, P11496). Sequencing library construction followed (61) ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were then analysed by agarose gel electrophoresis and purified using the GeneJET PCR purification kit (Thermo Scientific). The purified PCR products were subsequently cloned using the TOPO TA Cloning® kit (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE gels were loaded with 5-15 μL of lysate per lane (NuPAGE 4-12% Bis-Tris; Invitrogen). Lysates were diluted with additional 1x NuPAGE LDS buffer if necessary ...
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bioRxiv - Molecular Biology 2023Quote: ... The fragmented DNA samples were analyzed in a 1.5% agarose gel in TBE buffer and visualized using an Invitrogen iBright 1500 Imaging System (ThermoFisher).
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bioRxiv - Bioengineering 2024Quote: Protein samples were diluted in PBS to 5 μM and mixed with 5000× SYPRO Orange Protein Gel Stain (Invitrogen) to final 1.25x concentration ...
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bioRxiv - Plant Biology 2024Quote: ... 50 μg of sample was then loaded onto an SDS PAGE pre-cast 4-12% Bis Tris gel (ThermoFisher) and separated with MOPS buffer (ThermoFisher) ...