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Citations for Thermo Fisher :
7101 - 7150 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Gels were functionalized with 0.1M sulfo-succinimidyl 6-(4’-azido-2’-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Cat# 22589, Thermo Scientific) dissolved in HEPES before overnight incubation in collagen type-I solution (Catalogue # A1048301 ...
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bioRxiv - Microbiology 2024Quote: ... 30 μL of each concentrated sample was run on a Bolt 4–12% Bis-Tris Plus Gel (Thermo Scientific, catalog number ...
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bioRxiv - Immunology 2024Quote: ... Gel was prepared with 0.5 × TBE buffer with 11 mM MgCl2 and 0.005% v/v SYBR Safe (ThermoFisher #S33102), run at 70V for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... and aliquots containing 5 × 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen). The sample lane was divided into eight slices that were excised from the Coomassie-stained gel ...
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bioRxiv - Plant Biology 2023Quote: ... DNA degradation and quantity were assessed by resolving sample and standard DNA by agarose gel electrophoresis (Thermo Fisher Scientific). The isolated DNA was subjected to next-generation short read sequencing (NGS ...
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bioRxiv - Cancer Biology 2023Quote: ... An equal amount of total protein was resolved using SDS-PAGE gels and transferred to nitrocellulose membranes (Fisher Scientific) at 110mA for 2h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and uterine tissue lysates (75 μg per well) were subjected to electrophoresis in sulfate-polyacrylamide gels (Cat# NP0336BOX; Invitrogen). The proteins were transferred onto nitrocellulose membranes (Cat# 1620145 ...
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bioRxiv - Molecular Biology 2024Quote: ... and samples containing 30 µg total protein were analyzed by western blotting on 4-12% Bis-Tris Gels (Invitrogen). Proteins were transferred onto nitrocellulose membranes in accordance with the manufacturer’s instructions (Biorad) ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were transferred onto nitrocellulose membranes using an iBlot2 gel transfer device (Thermo Fisher Scientific, Waltham, MA, USA). The membranes were then blocked with 5% nonfat milk in PBST at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 10–15 µL of protein lysis were run per lane of 4–12% Bis-Tris gels (Thermo Fisher Scientific) using MOPS buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by electrophoresis in NuPage 4-12% Bis-tris gels run with 1X MOPS buffer (Invitrogen NP0323) and transferred to an iBlot nitrocellulose membrane (Invitrogen IB301002) ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg protein sample was loaded in each well and ran on a 4%–12% SDS-PAGE gel (Invitrogen), then transferred to the nitrocellulose membrane (0.2 µm ...
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bioRxiv - Cell Biology 2024Quote: ... while 10 μg/lane of lysates were electrophoresed on Bolt 4–12% Bis-Tris Plus Gels (Thermo Fisher Scientific) for β-actin ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then assessed the average fragment size within each extraction using 1.5x agarose gel and quantified the amount of double stranded DNA using a Qubit® 3.0 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... Double stranded DNA bands migrating at 490 nt were observed with SYBR Safe DNA Gel Stain (Thermo Scientific, S33102) were excised and extracted with a Freeze and Squeeze kit (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: ... The transcribed RNA was then separated on a 6% TBE-Urea (7M urea) polyacrylamide gel (Thermo Fisher Scientific, EC6865BOX) which was post stained with GelRed (Biotium ...
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bioRxiv - Microbiology 2024Quote: ... the bound proteins resolved using 4-12% Novex Tris-glycine gels and silver-stained (MS compatible) (Thermo Fisher Scientific). Trypsin digestions of the 0.2% FA elution were processed for mass spectrometric (MS ...
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bioRxiv - Immunology 2024Quote: ... The gel staining for protein content was done using Pierce™ silver stain/distain kit (Thermo Fisher Scientific 24612). Immunoblot analysis was performed using PVDF membranes (Pierce 88518 ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 5 minutes at 95°C and loaded on 4-12% Bis-Tris NuPage gels (Thermo Fisher Scientific). After electrophoretic separation ...
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bioRxiv - Cancer Biology 2024Quote: ... An equal amount of total protein was resolved using SDS-PAGE gels and transferred to nitrocellulose membranes (Fisher Scientific) at 110mA for 2h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The gels were transferred at 100 V for 1.5 hours at 4°C in transfer buffer (ThermoFisher Scientific, NP0006) onto PVDF membranes (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Gels were then run at 4°C in 0.5X TBE before staining in 1/10000 SYBR Gold (ThermoFisher Scientific) for 1 hour at room temperature on a rocker and visualization on the Typhoon 9400 Variable Mode Imager using the A488 channel.
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein per sample were separated on 4 to 20% Tris-glycine gradient gels (XP04200BOX, Thermofisher Scientific) and transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... the resulting traction force gels were coated with 30 ug/ml collagen type I using sulfo-SANPAH (Thermo Fisher) overnight at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... The quality and concentration of the linearized plasmid were confirmed via gel-electrophoresis and Nanodrop Spectrophotometer (Thermo Fisher Scientific). Following in vitro transcription to cRNA was performed using the linearized cDNA template and the mMessage mMachine® T7 transcription kit (Ambion ...
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bioRxiv - Biophysics 2024Quote: ... SDS-PAGE gel was transferred to PVDF membrane at 30 V for 45 min in NuPAGE Transfer Buffer (Invitrogen) and blocked with 4% BSA in TTBS [100 mM Tris/HCl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... The 2D well of the pre-cast NuPAGE 4%-12% Bis-tris 2D well gel (Thermo Fisher Scientific, NP0326BOX) was washed and filled with 1x MOPS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 15µg of each lysate prepared from muscle was loaded on NuPAGE 4-12% Bis-Tris acrylamide gels (Life Technologies) and run at 80V for 8 min to stack proteins in a single band ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were centrifuged at 13,300 rpm for 10 minutes and ran on NuPage 4-12% Bis-Tris polyacrylamide gels (Invitrogen). Proteins were transferred onto a Immobilon PVDF membrane (Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... 12 µL of each sample was loaded on an SDS-PAGE (Novex WedgeWell 10-20% Tris-Glycine gels, Invitrogen), run for 50 minutes at 200 V ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal volumes of normalized and heat-denatured protein preparations were electrophoresed on 12.5% SDS-PAGE gels alongside a SeeBlueTM Plus2 Prestained Standard (Invitrogen). VirB was detected using an affinity-purified anti-VirB antibody obtained from Pacific Immunology ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA quality check was done using gel electrophoresis and NanoDrop 1000 instrument (Thermo Fisher Scientific, Waltham, MA, USA). Quantitative estimation of DNA was performed using Qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were centrifuged at 13,300 rpm for 10 minutes and ran on NuPage 4-12% Bis-Tris polyacrylamide gels (Invitrogen). Proteins were transferred onto a Immobilon PVDF membrane (Millipore) ...
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bioRxiv - Neuroscience 2023Quote: ... Reducing and non-reducing protein samples were separated by gel electrophoresis and transferred to 0.2µm nitrocellulose membranes using the Bolt SDS-PAGE system (Life Technologies). Membranes were blocked in 5% nonfat dry milk (NFDM ...
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bioRxiv - Biophysics 2023Quote: ... The gels were run at 4°C using the electrophoresis system according to the Invitrogen instructions (ThermoFisher Scientific, USA), and then stained with the Pierce Silver Staining kit according to the manufacturer’s instructions (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... Gels were run at 200 V for approximately 1 h and incubated with SYBR Gold nucleic acid stain (Invitrogen), diluted 1:10,000 in TBE buffer (90 mM Tris ...
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bioRxiv - Synthetic Biology 2023Quote: ... The second-round PCR products were size-selected and purified using GeneJET Gel Extraction Kit (Thermo Fisher Scientific #K0691). The samples were pooled into a DNA LoBind 1.5-mL tube (Eppendorf #0030108051) ...
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bioRxiv - Cell Biology 2023Quote: ... RNA integrity was assessed by gel electrophoresis and RNA concentration was measured using a NanoDrop 2000 (Thermo Fisher Scientific). TaqMan Reverse Transcription Reagents (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and 1% n-Dodecyl-β-D-maltoside were loaded onto Novex 4-16% Bis/Tris gradient gels (Life Technologies). When assaying stoichiometry of AMPAR-auxiliary subunit complexes ...
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bioRxiv - Neuroscience 2022Quote: Forty micrograms of protein was heated in the loading buffer and loaded on 5–12% gradient NuPage gels (Invitrogen). After the transfer on nitrocellulose membranes ...
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bioRxiv - Immunology 2023Quote: ... Samples were subjected to SDS-PAGE on 4-12% Tris-Glycine Gels (Bio Rad) and transferred to a 0.2 μm nitrocellulose membrane using the iBlot system (Invitrogen) followed by blocking with 5% Milk in Tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Cell Biology 2023Quote: ... 25% of the total immunoprecipitated material (per condition) was loaded onto a 3-8% Tris-Acetate gel (Thermo Fisher), fully resolved gels were transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2023Quote: Protein extracts were loaded on pre-cast NuPAGE™ 10%, Bis-Tris (1.5 mm, 10-well) polyacrylamide gels (Invitrogen) and transferred onto Immobilon-P PVDF membranes (Merck Millipore) ...
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bioRxiv - Bioengineering 2023Quote: ... Gels were stained with Coomassie blue and evaluated against Novex sharp pre-stained protein marker (Thermo Fisher Scientific, USA). The imaging was done using Amersham Imager 600 RGB (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: The eluted proteins solubilized in Laemmli buffer were stacked in the top of a 4-12% NuPAGE gel (Invitrogen). After staining with R-250 Coomassie Blue (Biorad) ...
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bioRxiv - Microbiology 2023Quote: ... and loaded to gels (8 μL/well) for SDS-PAGE and Western blotting (NuPage Novex system, Thermo Fisher Scientific) to 0.45 μm nitrocellulose membranes (BioRad laboratories).
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bioRxiv - Microbiology 2022Quote: ... heated for 10 min at 95°C and separated on 4–12% Bis-Tris NuPAGE gels (Thermo Fisher Scientific) in the presence of antioxidant (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein (between 10 and 20 ug) was resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE; ThermoFisher #NP0335) in 1X MOPS buffer (40 mM 3-(N-morpholino ...
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bioRxiv - Microbiology 2023Quote: ... and pooled at equimolar concentrations before single-tube cleaning with the Invitrogen Gel and PCR Clean-up Kit (Invitrogen) and sequencing using an Illumina MiSeq (v2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The digested RNA was further resolved by 10% urea polyacrylamide gel electrophoresis (PAGE) followed by SYBR Gold (Invitrogen S11494) staining.