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Citations for Thermo Fisher :
7051 - 7100 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... were added to the wells and ran on a NuPage™ 4-12% Bis-Tris mini gel (Invitrogen, NP0336BOX). After gel electrophoresis ...
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bioRxiv - Neuroscience 2022Quote: ... RNA integrity and yield were assessed by 1.5% agarose gel electrophoresis and UV-visible spectroscopy (NanoDrop, Thermo Fisher Scientific), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... was added to the samples and the samples transferred to a NuPAGE 4-12% Bis-Tris Gel 1.0 mm x 10 well (Invitrogen) for SDS PAGE gel electrophoresis ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were incubated at 37°C for 30 min and loaded onto 6% DNA retardation gels (Thermo Fisher Scientific). DNA was detected by staining the gel with GelRed (VWR) ...
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bioRxiv - Biophysics 2022Quote: DNA fragment size of inputs was checked on 1X TBE 2.5% agarose gels with 1X SYBR Safe (Invitrogen, S33102) to ensure MNase digestion ...
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bioRxiv - Cell Biology 2022Quote: ... Sample buffer was added and the proteins resolved by electrophoresis on a NuPAGE 10% BisTris mini gel (Thermo Fisher). The gel was stained using SimplyBlue Safestain (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA (7.5 μg) was separated on a formaldehyde denaturing 1% agarose gel and transferred to a BrightStar-Plus membrane (Ambion) using capillary blotting ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 µg protein of each sample was loaded into 4 to 12% Bis-Tris protein gel (Thermo Fisher Scientific) and sent for MS analysis at the University of Texas System Proteomics Network ...
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bioRxiv - Immunology 2022Quote: ... 20 μg of total protein lysates were loaded and separated on NuPAGE 4–12% Bis-Tris gels (Thermo Scientific). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and quality was assessed on a 1% agarose gel before quantification using the Qubit dsDNA HS Assay Kit (Invitrogen). Library preparation and subsequent RRBS sequencing were carried out at SciLife (Uppsala University ...
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bioRxiv - Microbiology 2022Quote: ... LPS was visualized by staining with the Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (Molecular Probes, Eugene, OR).
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bioRxiv - Microbiology 2022Quote: ... the resulting DNA fragments were separated by loading 10 µl of PCR product onto a 1.5% agarose gel stained with ethidium bromide (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Western blot was performed according to the manufacturer guidelines using Novex 10-20% Tris-Glycine Mini Gels (XP10202BOX, ThermoFisher). Proteins were subsequently electrotransferred onto a polyvinylidene difluoride membrane (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... and BN-PAGE gels were run for 2-2.5 hours at 150 V using NativePAGETM running buffer (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... was loaded on a NuPage 4-12% gradient Bis-Tris pre-cast SDS-PAGE gel (NP0321, Thermo Fisher Scientific), which was electrophoresed at 115 V for 90 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... The 70kd Ssa1,2 proteins were isolated by electrophoresis on an SDS polyacrylamide gel followed by staining with colloidal coomassie blue (Invitrogen). The 70kd band was excised from the gel and subjected to reduction ...
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bioRxiv - Genomics 2022Quote: RNA concentration and quality were determined by gel electrophoresis in 1% UltraPure™ agarose (Invitrogen Corp., Carlsbad, California, USA) in 1X TBE running buffer at 100 V for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... 5 μg total RNA were denatured for 5 mins at 95°C in RNA Gel loading dye (Thermo Scientific) before being separated on 1% agarose gels in 1X TBE (native ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates containing 20 μg protein were then resolved on NuPAGE 4-12% Bis-Tris gels (Thermo Fisher Scientific NP0323) and transferred to PVDF membranes (Bio-Rad 1620177) ...
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Post-secretory synthesis of a natural analog of iron-gall ink in the black nectar of Melianthus spp.bioRxiv - Plant Biology 2022Quote: ... Concentrated nectar proteins were evaluated using denaturing 4-20% SDS-PAGE gels stained with PAGE-Blue (Thermo Fisher #24620).
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bioRxiv - Plant Biology 2022Quote: ... RNA quality and concentration were checked on a 0.9% agarose gel and by NanoDrop (Thermo Fisher Scientific, Vantaa, Finland). Single-strand DNA (cDNA ...
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bioRxiv - Microbiology 2024Quote: ... 30 μL of each concentrated sample was run on a Bolt 4–12% Bis-Tris Plus Gel (Thermo Scientific, catalog number ...
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bioRxiv - Immunology 2024Quote: ... Gel was prepared with 0.5 × TBE buffer with 11 mM MgCl2 and 0.005% v/v SYBR Safe (ThermoFisher #S33102), run at 70V for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were functionalized with 0.1M sulfo-succinimidyl 6-(4’-azido-2’-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Cat# 22589, Thermo Scientific) dissolved in HEPES before overnight incubation in collagen type-I solution (Catalogue # A1048301 ...
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bioRxiv - Physiology 2022Quote: ... The supernatant was mixed with LDS loading buffer and then resolved in a gradient NuPAGE gel (4%-12%, Invitrogen). NuPAGE gel resolved proteins were transferred to PVDF membranes at 30 V overnight at 4 C ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluates were loaded on a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and analysed with IstantBlue staining (Merck).
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bioRxiv - Developmental Biology 2022Quote: ... Samples were assessed by silver staining using NuPAGE 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific, Cat# NP0335) and SilverQuest Silver Staining Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The PAA gel surface was then incubated with a solution of 2 mg ml−1 Sulpho-SANPAH (4822589, ThermoFisher) under ultraviolet light for 5 min (wavelength of 365 nm at 5 cm distance) ...
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bioRxiv - Genomics 2022Quote: ... Quality and quantity of the DNA were measured via standard agarose-gel electrophoresis and with a Qubit 3.0 Fluorometer (Invitrogen). Nanopore sequencing libraries of S ...
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bioRxiv - Genetics 2022Quote: ... All samples were loaded on pre-cast NuPAGE™ 10%, Bis-Tris (1.5 mm, 10-well) polyacrylamide gels (Invitrogen) and transferred onto Immobilon-P PVDF membranes (Merck Millipore) ...
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bioRxiv - Plant Biology 2022Quote: ... and qualified and quantified using 1% agarose gel and a Qubit™ Fluorometer 4 (Invitrogen, Life Technology, CA, USA). DNA was randomly fragmented using Bioruptor® Pico B01060010 (Diagenode ...
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bioRxiv - Molecular Biology 2022Quote: ... Gels were run at 100V until completion of electrophoresis and visualised using colloidal coomassie blue (Thermofisher Scientific, Paisley, UK) according to manufacturer’s guidelines.
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bioRxiv - Microbiology 2022Quote: ... Gel remnants were removed by centrifugation (10 min, 1.000xg) and protein content was measured with BCA assay (ThermoFisher, US). Samples were normalized to 100μg/ml with ddH2O ...
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bioRxiv - Cell Biology 2022Quote: ... samples were separated on 10% or 4-20% gradient Novex™ WedgeWell™ Tris-Glycine gels (Thermo Fisher Scientific) for 40 minutes at 225V and transferred at 40V overnight at 4°C onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were heated to 80°C for 5 min and electrophoresed on 4-12% NuPAGE Bis-Tris gels (ThermoFisher). Wet transfer was to a nitrocellulose membrane (0.45 uM pore ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were transferred onto nitrocellulose membranes using an iBlot2 gel transfer device (Thermo Fisher Scientific, Waltham, MA, USA). The membranes were then blocked with 5% nonfat milk in PBST at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... An equal amount of total protein was resolved using SDS-PAGE gels and transferred to nitrocellulose membranes (Fisher Scientific) at 110mA for 2h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Gels were then run at 4°C in 0.5X TBE before staining in 1/10000 SYBR Gold (ThermoFisher Scientific) for 1 hour at room temperature on a rocker and visualization on the Typhoon 9400 Variable Mode Imager using the A488 channel.
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bioRxiv - Biophysics 2024Quote: ... The quality and concentration of the linearized plasmid were confirmed via gel-electrophoresis and Nanodrop Spectrophotometer (Thermo Fisher Scientific). Following in vitro transcription to cRNA was performed using the linearized cDNA template and the mMessage mMachine® T7 transcription kit (Ambion ...
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bioRxiv - Biophysics 2024Quote: ... SDS-PAGE gel was transferred to PVDF membrane at 30 V for 45 min in NuPAGE Transfer Buffer (Invitrogen) and blocked with 4% BSA in TTBS [100 mM Tris/HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein per sample were separated on 4 to 20% Tris-glycine gradient gels (XP04200BOX, Thermofisher Scientific) and transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... the resulting traction force gels were coated with 30 ug/ml collagen type I using sulfo-SANPAH (Thermo Fisher) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The gels were transferred at 100 V for 1.5 hours at 4°C in transfer buffer (ThermoFisher Scientific, NP0006) onto PVDF membranes (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The 2D well of the pre-cast NuPAGE 4%-12% Bis-tris 2D well gel (Thermo Fisher Scientific, NP0326BOX) was washed and filled with 1x MOPS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µL of each sample was loaded into a well of NativePAGE 4-12% Bis-Tris Gels (Invitrogen NP0321BOX). The gels were run at 150 V for approximately two hours at 4 °C and transferred onto 0.2 µm PVDF membranes (Millipore IPVH00010 ...
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Modular structure of RNA 3’ processing condensates involving the Arabidopsis RNA binding protein FCAbioRxiv - Molecular Biology 2024Quote: ... The band at the right size was cut out, sliced, and eluted in gel extraction buffer (0.5 M NH4Ac, 1 mM EDTA, 0.2% SDS, 1xRNAsecure™ (Invitrogen)) at 37ºC overnight ...
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bioRxiv - Molecular Biology 2024Quote: Equivalent volumes of each fraction were loaded onto a 4-12 % Bis-Tris NuPAGE SDS-PAGE gel (Life Technologies) and separated by molecular weight using denaturing electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... Labeling reactions were incubated for 30 min at 37°C and stopped with the addition of 2X denaturing gel loading buffer (95% Formamide, 18 mM EDTA, 0.025% SDS, Xylene Cyanol, Bromophenol Blue; ThermoFisher).
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bioRxiv - Neuroscience 2024Quote: ... from each sample were separated on 10% Tris-HCL SDS-PAGE gels and transferred to a nitrocellulose membrane (0.45 µm pore size, #LC2001, Invitrogen). Blots were blocked with fish gelatin (Biotium ...
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bioRxiv - Microbiology 2024Quote: ... Purified proteins were examined using NuPAGE Bis-Tris 4-12% acrylamide gels with MES as the running buffer (Invitrogen). Proteins were stained AcquaStain Protein Gel Stain (Bulldog-Bio).