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Citations for Thermo Fisher :
7001 - 7050 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... 10-100 μg total protein was then separated with a 4-12% (v/v) gradient Bis-Tris gel (ThermoFisher) run in MES buffer system ...
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bioRxiv - Immunology 2021Quote: ... Samples were resolved by electrophoresis on 4-12% Bis-Tris gels with MOPS Running Buffer (Life Technologies NuPAGE system) and transferred to nitrocellulose membrane by electrophoresis at 100V for 1 hour in cold transfer buffer (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... Gel purified cDNA was subjected to in vitro transcription using the MEGAscript T7 High Yield Transcription Kit (ThermoFisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Cell lysates and immunoprecipitations were resolved using precast Novex 4-12% Bis-Tris Midi Protein Gels (Thermo Fisher Scientific) and transferred to methanol-activated Immobilon-P PVDF Membranes (Millipore ...
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bioRxiv - Physiology 2019Quote: ... Phalloidin-FITC was aspirated and gels were washed 3 × TBS before adding 250 μl of DAPI stain (ThermoFisher, D1306) at a concentration of 300 μM for 90 mins to counterstain myonuclei ...
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bioRxiv - Cancer Biology 2020Quote: In-gel and SCX fractions were analyzed using a Q Exactive or Orbitrap Elite mass spectrometer (Thermo Fisher Scientific), respectively ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA quality was tested using agarose gel electrophoresis and concentration was quantified using a Nanodrop Lite spectrophotometer (Thermo Fisher). Complementary DNA was created by reverse transcription using an iScript cDNA kit (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: ... Thirty micrograms of protein lysates were resolved on Bolt 4-12% Bis-Tris plus gel (Invitrogen, Carlsbad, CA, USA) under reducing conditions and transferred to a nitrocellulose filter ...
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bioRxiv - Immunology 2019Quote: Twenty microgram (µg) of total protein lysates were size-fractionated on 4-12% NuPAGE Bis-Tris gel (#NP0321, Invitrogen). OLR1 was detected by incubation with a rabbit anti-mouse OLR1 antibody (1:500 ...
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bioRxiv - Plant Biology 2019Quote: ... About 20 µg of total soluble proteins of each sample was loaded to 3-12% Bis-Tris 1.0 mm Invitrogen NativePAGETM protein gels from ThermoFisher Scientific ...
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Exploring the microbial diversity in oil-contaminated mangrove sediments using 16S rRNA metagenomicsbioRxiv - Microbiology 2019Quote: ... DNA was visualised on a 0.8% agarose gel and quantified using a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA). DNA from all samples were pooled together and the DNA was concentrated to a final volume of 50 µl ...
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bioRxiv - Genomics 2021Quote: The beads were magnetically separated and the eluate was loaded on a 4-12% NuPAGE Bis-Tris gel (Invitrogen). 0.5 l of 1x MOPS running buffer (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... Samples (5 μL each) were loaded into wells of a NuPAGE Tris-acetate 3 – 8% polyacrylamide gel (ThermoFisher Scientific) and electrophoresed for 60 min at 15 volts/cm ...
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bioRxiv - Cell Biology 2020Quote: ... The immunoprecipitates were dissolved in 2×SDS loading buffer and resolved on NuPAGE 4–12% Bis-Tris gel (Invitrogen), and then silver stained using Pierce silver stain kit (Thermo) ...
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bioRxiv - Microbiology 2020Quote: ... and separated on 10% polyacrylamide gels alongside dual-color Page Ruler Prestained Protein size markers (Thermo Fisher Scientific, Poland). The separated proteins were then transferred onto a Westran S PVDF membrane (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: The input (total Nav1.5 expression) and biotinylated (cell surface-expressed Nav1.5) samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen, ThermoFisher) and transferred to nitrocellulose membrane (iBlot transfer system ...
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bioRxiv - Biophysics 2021Quote: ... and incubated (95 °C, 20 min) before SDS-PAGE using 3–8 % Tris-Acetate protein gels (Thermo Fisher Scientific). After transfer onto PVDF membranes (iBlot 2 Dry Blotting System ...
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bioRxiv - Genomics 2021Quote: The integrity and concentration of RNA and DNA was analyzed by agarose gel electrophoresis and Qubit 2.0 fluorometer (ThermoFisher), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... phosphorylation reactions were stopped by adding SDS-containing sample buffer and samples were analyzed using a SDS-polyacrylamide gel supplemented with 2,2,2-trichlorethanol (TCE, Acros Organics) for stain-free visualization (Ladner et al ...
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bioRxiv - Biochemistry 2021Quote: ... and for nucleic acids using a 12.5 % polyacrylamide/8 M urea gel visualized by SYBR Gold staining (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... 1x samples were run on a 1 % Agarose gel (BP1356-500, Fisher Bioreagent, Fisher Scientific, Hanover Park, IL, USA) in 1x TAE buffer at 110 V for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were boiled at 95°C for 5 minutes and 15–30μl was separated on Tris-Glycine SDS-PAGE gels (ThermoFisher) followed by Western blotting onto nitrocellulose membranes (ThermoFisher ...
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bioRxiv - Genomics 2021Quote: ... The quality and quantity of the DNA were measured via standard agarose gel electrophoresis and with a Qubit□4.0 Fluorometer (Invitrogen). Sequencing library was constructed and sequenced by Nanopore PromethION platform (Oxford Nanopore Technologies) ...
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bioRxiv - Genomics 2021Quote: ... The quality and quantity of the DNA were measured via standard agarose gel electrophoresis and with a Qubit□2.0 Fluorometer (Invitrogen). Sequencing library was constructed and sequenced by Illumina Novaseq platform (Illumina).
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bioRxiv - Cancer Biology 2021Quote: ... which were boiled for 5 min at 95°C and then separated on 4-12% polyacrylamide gradient gels (Invitrogen). After blotting ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were separated by electrophoresis in NuPAGE 4-12% Bis-Tris gels run with 1x MOPS buffer (Invitrogen NP0323) and transferred to an iBlot nitrocellulose membrane (Invitrogen IB301002) ...
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bioRxiv - Genetics 2020Quote: ... thirty micrograms of whole brain extracts were electrophoresed in 10-20% Tris-Glycine Gel (Novex™ WedgeWell™, Invitrogen) and transferred to Immun-Blot™ PVDF membranes (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... RNA integrity and concentration were confirmed by agarose gel electrophoresis and by using the Nanodrop 2000 (Thermo Fisher Scientific) respectively ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein was loaded into a 15-well NuPAGE 4-12 % Bis-Tris gel (Cat# NP0336BOX, ThermoFisher) and run in MES buffer (50 mM MES ...
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bioRxiv - Bioengineering 2020Quote: ... gels were detached from their fixed points and mounted on poly-l-lysine coated microscope slides (Fisher Scientific, UK) and the constructs were ringed with PAP pen (Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... DNA quality was confirmed by agarose gel electrophoresis and quantification was performed on a NanoDrop 8000 spectrophotometer (Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... and 5µl of the diluted samples were loaded onto a 4-12% bis-tris NuPage gel (Thermo Fisher Scientific) and run at 200V for 50min in MOPS buffer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... The libraries were subjected to size selection (250–500 bp) on a 4–20% TBE PAGE gel (Thermo Scientific). The recovered libraries were sequenced on the Illumina Nextseq platform using Nextseq 500 mid-output kit with 2×150 paired-end.
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bioRxiv - Molecular Biology 2020Quote: ... The libraries were subjected to size selection (250–500 bp) on a 4–20% TBE PAGE gel (Thermo Scientific). The recovered libraries were sequenced on the Illumina Nextseq platform using Nextseq 500 high-output kit with 2 × 76 paired-end ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 5 μg protein was run on a NuPage™ 4-12% Bis-Tris Protein Gel (Invitrogen) using MOPS buffer according to manufacturer instructions and subsequently transferred to a nitrocellulose membrane according to the NuPage™ manufacturer's protocol (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: Protein samples (4×106 cell equivalents per lane) were separated on 4%-12% Bis-Tris gels (Thermo Fisher Scientific) and then transferred to nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 2 μg of protein per lane was loaded on a 4-12% graded Tris-glycine SDS-polyacrylamide gel and proteins were transferred to a 0.2μm PVDF membrane (Life Technologies). Membranes were blocked in 5% milk in PBS with 0.05% Tween-20 for one hour at room temperature with rocking ...
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bioRxiv - Immunology 2021Quote: ... lysed in 1X Novex buffer and 5-7.5×105 cells equivalents were resolved on 4-12% NuPAGE gel (Invitrogen) in MOPS buffer ...
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bioRxiv - Immunology 2021Quote: ... The proteins from the SDS-PAGE gel were blotted onto 0.2 µm nitrocellulose (NC) membrane (#LC2000, Invitrogen, Thermofisher Scientific) by wet transfer ...
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bioRxiv - Immunology 2021Quote: ... The proteins from the SDS-PAGE gel were blotted onto 0.2 µm nitrocellulose (NC) membrane (#LC2000, Invitrogen, Thermofisher Scientific) by wet transfer ...
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bioRxiv - Genetics 2019Quote: ... 10% β-mercaptoethanol at 95°C for 5 min and run on NuPage 10% Bis-Tris gels (Life Technologies) in 2-(N-morpholino ...
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bioRxiv - Genomics 2021Quote: ... Gels were stained for 3 min with SYBR Gold Nucleic Acid Gel Stain (diluted from 10,000x in 1x TE; Invitrogen) and visualized by UV transillumination ...
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bioRxiv - Biochemistry 2020Quote: ... 50-75 μg of proteins were loaded on Pre-cast Native PAGE 3-12% Bis-Tris gels (Invitrogen, #BN2011BX10) at 150 V and 4°C for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Precipitates were resolved in RNase-free water for visualization on 12.5% denaturing acrylamide gel with SYBR Gold (Thermo Scientific). Random hexamer primers were used for preparing TruSeq3 reverse-stranded cDNA libraries for Illumina single-end 50bp sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... Reducing and non-reducing protein samples were separated by gel electrophoresis and transferred to 0.2μm nitrocellulose membranes using the Bolt SDS-PAGE system (Life Technologies).
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bioRxiv - Immunology 2022Quote: ... The soluble fraction was supplemented with 0.5% Coomassie G-250 immediately before loading samples onto NativePAGE 4 to 16% Bis-Tris mini gels from Invitrogen™ ...
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bioRxiv - Microbiology 2022Quote: ... The final sequencing template for each group (187-bp in length) was purified using SizeSelect II agarose gels (Invitrogen) and analysed using a Bioanalyzer High Sensitivity DNA chip (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... quantified using aLife Technologies Qubit 2.0 instrument were run on 4-12% Bis-Tris Plus Bolt gels (ThermoFisher Scientific) and transferred to a nitrocellulose membrane with an iBlot dryblot system (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were heated to 95°C for 10 min and run on NuPAGE 4%-12% bis-tris gels (Invitrogen). Proteins were transferred to PVDF membranes (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR-products were separated on a 1.5-2.5% agarose gel along with molecular weight ladder (ThermoFisher, Waltham, MA), stained with 0.5µg/mL ethidium bromide (Research Products International ...