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Citations for Thermo Fisher :
6951 - 7000 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... mRNA integrity prior to and after formulation was analyzed on 1% agarose gel with SYBR safe stain (Thermo Fisher).
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bioRxiv - Immunology 2022Quote: ... The quality and quantity of RNA were evaluated by agarose gel electrophoresis and Qubit RNA HS Assay Kit (Invitrogen).
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bioRxiv - Developmental Biology 2022Quote: ... 20μg of each respective sample was loaded onto a 1mm 3-8% Tris-Acetate Mini Protein gel (NuPAGE) along with 10μl of Spectra Multicolor High Range protein ladder (Thermofisher). The gel was run at 80V for 15 minutes and then increased to 120V until the dye front reached the bottom ...
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bioRxiv - Cell Biology 2022Quote: Gel bands were analysed using a LTQ-Orbitrap Velos Pro mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... The cells were washed from the slides into 1 mL of cold 0.1X PBS via pipette and stained with SYBR Green I nucleic acid gel stain (Invitrogen) for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... RNA quality and quantity were assessed using gel electrophoresis and using a NanoDropTM 2000/2000c Spectrophotometer (Thermo Fisher Scientific). All RNA extractions were carried out in triplicate.
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bioRxiv - Molecular Biology 2022Quote: ... The size and integrity of the biotinylated RNA were checked on a 6% Urea gel (Thermo Scientific, Darmstadt, Germany). Immediately before use ...
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bioRxiv - Plant Biology 2022Quote: ... The gel was then transferred to Hybond N+ (Amersham) nylon membrane and blotted with HRP-Conjugated Streptavidin (Thermo Scientific) with a dilution factor 1:2000 ...
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A quantitative tri-fluorescent yeast two-hybrid system: from flow cytometry to in-cellula affinitiesbioRxiv - Biochemistry 2019Quote: ... Ten microliters were used for SDS-Page analysis on Bolt™ 4-12% Bis-Tris Plus Gels (Thermo Scientific). Electrophoresis separation was performed in NuPAGE™ MOPS SDS Running Buffer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell extracts (20 μg per lane) were separated by NuPAGE™ 4-12% Bis-Tris protein gels (ThermoFisher Scientific) and transferred to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2019Quote: ... The gel was stained with 1 mg/ml Coomasie blue (Coomasie Brilliant Blue™ R-250 from ThermoFisher Sci.). The destained gel was stored in 5% glacial acetic acid at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... 20 μg total protein for each sample was run on 4-12% Bis-Tris gels (Thermo Fisher Scientific NP0322) with MES buffer (Thermo Fisher Scientific NP002) ...
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bioRxiv - Neuroscience 2019Quote: ... or slices (Figure 2E) were embedded very soft (0.2–0.3 mg/ml) collagen gel containing red fluorescent microbeads (Fluoro-Max [Thermo Fisher Scientific] ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using Bradford reagent and samples were run on a 4-12% Tris/Bis gel (Invitrogen). Protein was then transferred to an Immobilon-P PVDF membrane (Millipore) ...
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bioRxiv - Molecular Biology 2019Quote: ... SDS-PAGE was performed on NuPAGE 4–12% Bis–Tris gels with NuPAGE MOPS SDS Running Buffer (Life Technologies). Following transfer of proteins to nitrocellulose membranes ...
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bioRxiv - Genetics 2019Quote: ... The quality and concentration of the extracted DNA was verified using gel electrophoresis and Qubit 2.0 fluorometer (Life Technologies). Extracted genomic DNA was used for preparing Illumina RAD sequencing libraries based on previously described protocols (Baird et al ...
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bioRxiv - Synthetic Biology 2020Quote: ... 4 µl CFE reaction was loaded onto a NuPAGE 4–12% Bis–Tris Gel (Life Technologies, Carlsbad, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The gel-purified PCR product was then cloned into the sub-cloning vector TOPO XL using manufacturer’s instructions (Invitrogen). We amplified and cloned genomic fragments from resistant (Bowman+Rph15 and Hs 680 ...
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bioRxiv - Cancer Biology 2020Quote: ... denaturated 5 min at 95°C and finally loaded on NuPAGE 4-12% Bis-Tris protein gels (Life Technologies) and electro-transferred onto nitrocellulose membranes ...
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bioRxiv - Plant Biology 2019Quote: ... DNA quality was checked on a 1.5% agarose gel and DNA quantity was measured with Qbit HS (Invitrogen, USA). Overall ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Reactions were incubated for 60 min at room temperature and run on a 6% DNA Retardation gel (Invitrogen™) in TBE buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Elution fractions were analyzed by reducing SDS-PAGE using precast NuPAGE 4-12% Bis-Tris gels (Thermo Fisher Scientific) with 1x MOPS buffer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... while proteins above 200 kDa were blotted on a 3-8% Tris-Acetate gels in MOPS Buffer (Life Technologies). Proteins were separated at 200V for approximately one hour and transferred onto either PVDF or nitrocellulose membrane using the TransBlot Turbo Transfer (BioRad ...
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bioRxiv - Cell Biology 2019Quote: ... Each library was size-selected for 200-300 bp fragments on a 1.5% agarose gel and the library concentrations were determined using a QuBit 3.0 Fluorometer (Thermo Scientific). Libraries were sequenced on an Illumina HiSeq2000 using single-end 50 bp sequencing at the University of Massachusetts Medical School deep sequencing core facility.
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bioRxiv - Molecular Biology 2019Quote: ... was added to the plasmid sample prior to resolving it on a 0.7% agarose gel stained with SYBR gold (Invitrogen).
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bioRxiv - Microbiology 2019Quote: ... DNA was visualized on a 0.8 % agarose gel and quantified using the Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA). Extracted DNA was stored at −80 °C.
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bioRxiv - Cell Biology 2019Quote: ... and incubated for 5min at 90°C before loading on pre-cast 4-12% Bis-Tris acrylamide gel (Invitrogen). Subsequently ...
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bioRxiv - Immunology 2019Quote: ... Nuclear lysates (10µg per lane) were resolved on precast NuPAGE 4-12% Bis-Tris protein gels (Invitrogen, Carlsbad, CA) and proteins transferred to PVDF membranes (Bio-Rad ...
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bioRxiv - Synthetic Biology 2020Quote: ... un-incorporated GMPS and DTT were removed by gel filtration using two Zeba Spin desalting columns (Thermo Fisher Sci.) sequentially.
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An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccinesbioRxiv - Microbiology 2020Quote: ... BN-PAGE gels were run for 2 hours at 150 V using the NativePAGE™ running buffer (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2020Quote: ... Each 3D-EHT was casted in a mold made of 2% agarose by adding 500,000 WTC11c hiPSC-CMs and 50,000 HS27a stromal cells in a fibrin gel solution (89 μL RPMI-1640 supplemented with B-27, 5.5 μl of DMEM/F12, [Gibco] ...
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bioRxiv - Cancer Biology 2020Quote: ... The beads with antigens captured were loaded onto a SDS-PAGE gel (4-20% gradient polyacrylamide) (Thermo Fisher Scientific) in duplicates ...
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bioRxiv - Genetics 2020Quote: ... A 30 μg sample of protein was loaded and resolved on a 10% NuPAGE BIS-TRIS gel (Life Technologies), then transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Systems Biology 2020Quote: LC-MS/MS acquisition in samples of 30µg of 3,910 proteins was prepared from the groups of patient biopsies running on a NUPAGE 4-12% acrylamide gel (Invitrogen) and stained in Coomassie blue (Simply-blue Safestain ...
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bioRxiv - Immunology 2020Quote: Lysate of Bacillus anthracis was loaded on a 4-12 % gradient NuPAGE Novex SDS-PAGE gel (Invitrogen, Thermo Fisher). After migration ...
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bioRxiv - Immunology 2020Quote: Lysate of Bacillus anthracis was loaded on a 4-12 % gradient NuPAGE Novex SDS-PAGE gel (Invitrogen, Thermo Fisher). After migration ...
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bioRxiv - Molecular Biology 2020Quote: ... the annealing reaction mixture was analysed using a 4% agarose gel containing a DNA stain (Thermo Fisher Scientific, #S33102). The bands were visualized using a UV transilluminator (Bio-Rad laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... The silver-stained gel shown in Figure S1A and H were generated using Pierce Silver Stain Kit (ThermoFisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... Cells were prepared for analysis by staining with 2 x SYBR Green I nucleic acid gel stain (Life Technologies) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... SDS–PAGE and immunoblotting were performed using the 4-12% NuPage gel system (Life Technologies, Foster City, CA, USA). Membranes were blocked in 5% milk/TBS-Tween ...
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bioRxiv - Molecular Biology 2020Quote: ... heated at 95°C for 3 min and seprated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to an PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... SDS-PAGE gels were loaded with 5 - 15 μL of lysate per lane (NuPAGE 4-12% Bis-Tris; Invitrogen). Lysates were diluted with additional 1x NuPAGE LDS buffer if necessary ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were run on Novex 4-12 % gradient gel (Bis-Tris) in 1x NuPAGE™ MES buffer (Invitrogen) at 150 V for 60 min on ice followed by 30 min at 200 V ...
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bioRxiv - Microbiology 2020Quote: ... Ten μg of protein per sample was loaded on NovexTM 10-20% Tris-Glycine protein gels (Thermo Fisher Scientific) and transferred onto iBlot2 Transfer Stacks PVDF membrane (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The isolated DNA was assessed with a 1.0% agarose gel and measured with a Qubit 2.0 Fluorometer (Invitrogen, Life technologies) using Broad-range assay reagents ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were polymerized for 2 h at 37°C and then digested with 8 U/ml Proteinase K (Invitrogen) in 50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2020Quote: Proteins were separated by SDS-PAGE using NuPAGE 4–12% (w/v) Bis-Tris gradient gels (Thermo Fisher Scientific), and then transferred onto a PVDF membrane using the iBlot 2 dry blotting system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The integrity of DNA was checked on gel electrophoresis and quantified using a NanoDrop ND1000 Spectrophotometer (Thermo Scientific, USA).
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using a Gel Extraction Kit (OMEGA, D2500-01) and quantified with Qubit 4 fluorometer (Invitrogen). The amplicon library was sequenced by Illumina MiSeq (500-cycle ...
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bioRxiv - Neuroscience 2020Quote: All proteins were separated on pre-cast 4-12 % Bis-Tris SDS-PAGE gels (NPO321BOX, NPO322BOX, NPO336BOX) from Invitrogen using MES running buffer (50mM MES ...