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Citations for Thermo Fisher :
6901 - 6950 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 7.5 μl of 6 μM samples were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Gel, Life Technologies) or native gels (NativePAGE 4-16% Bis-Tris Gel ...
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bioRxiv - Cell Biology 2021Quote: After Galectin 3 immunoprecipitation the samples were resolved on SDS-NuPAGE gel and stained with Simply Safe Blue (Invitrogen). Multiple protein bands were excised ...
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bioRxiv - Microbiology 2021Quote: ... we pooled the tRNA samples as described above but ran the pool on a 10% TBE-Urea gel (Invitrogen) cutting just above a primer dimer band present in a no template control up to ∼150 nt by comparison to a dsDNA ladder ...
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bioRxiv - Cell Biology 2021Quote: ... The gel solution was pipetted into 4-well chamber slides (Nunc Lab-Tek Chamber Slide Systems ™, ThermoFisher Scientific) and gels were permitted to crosslink for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... and equal loads of total protein were separated on a NuPAGE 4-12% Bis-Tris mini gel (NP0336BOX, ThermoFisher) and then transferred onto a Biotrace NT nitrocellulose membrane (27376-991 ...
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bioRxiv - Cancer Biology 2022Quote: ... Gel electrophoresis was performed at 130 V for 70 min in Bolt MOPS SDS Running Buffer (ThermoFisher Cat# B0001). Proteins were subsequently transferred to a 0.45 μm polyvinylidene difluoride membrane ...
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bioRxiv - Cell Biology 2022Quote: The lysates were analyzed by 4-16% Native PAGE using precast Bis-Tris Mini Protein Protein Gels (Invitrogen BN1003BOX) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... the reaction of the supercoiled DNA on a 1.5% agarose 1x TAE gel and visualized using SYBR-Safe (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... Forty μg of protein extract for each sample were separated on a 4–12% NuPage Bis-Tris gel (Invitrogen) at 125 V for 100 min in NuPage MES SDS Running Buffer (Invitrogen)and transferred to nitrocellulose membranes.
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bioRxiv - Pathology 2022Quote: ... Samples were diluted in 2× sample buffer (see above) and loaded onto 10% SDS-polyacrylamide gels (Invitrogen, Waltham, MA), followed by electrophoresis and Western blotting on to PVDF membranes (Hybond P ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 µg of RNA was electrophoresed in a 1.2 % denaturing formaldehyde agarose gel with NorthernMax reagents (Thermo Fisher Scientific). The gel was washed twice in nuclease free water (15 min each ...
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bioRxiv - Biochemistry 2022Quote: ... Fifteen to 20 μg of protein was loaded into a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies) and transferred onto a nitrocellulose membrane with the iBlot 2 semidry transfer system (Life Technologies) ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 mM aminocaproic acid and 30% sucrose) and loaded onto a 4-16% precast BN-PAGE gel (Life Technologies). Second dimension analysis was performed by solubilizing BN-PAGE gel slices in 2x Laemmli buffer (Laemmli ...
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bioRxiv - Neuroscience 2022Quote: ... Total protein in solutions was quantified prior to gel loading using standard BCA assay (Pierce™, Cat#23225, Thermofisher) following manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Denatured protein lysates (12.5 μg/sample) were loaded on to NuPAGE 4-12% Bis-Tris 1.0 mm Mini Protein Gels (Invitrogen, NP0321) alongside Chameleon Duo Protein Ladder (3 μL/lane ...
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bioRxiv - Biochemistry 2022Quote: ... and heated at 95°C for 5 min before loading onto a NuPAGE Bis-Tris 4-12% gel (Invitrogen). Immunoblotting was performed using a Mini Trans-Blot cell (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... RNAs from both treated and untreated arms of the experiment were separated on a 5% denaturing acrylamide:bisacrylamide (19:1) gel alongside a RiboRuler Low Range RNA Ladder (ThermoFisher) and the gel fixed ...
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bioRxiv - Bioengineering 2022Quote: ... SDS-PAGE was performed under reducing conditions using Novex 12% Tris-Glycine Midi Gels (Thermo Fisher Scientific, Waltham, MA). Separation was performed at 125V for 90 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl of the supernatant was used for SDS-PAGE on 8-12% Tris-Glycine gels (NOVEX™, Invitrogen) with Tris-Glycine SDS Running buffer (NOVEX™ ...
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bioRxiv - Cell Biology 2022Quote: ... The MV protein fractions were detected on the protein gel using Simple-Blue™ Safe Stain (ThermoFisher Scientific, UK). The sandwich method was then used to transfer the proteins from the gel to a methanol-activated polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was developed on film or Bio-Rad gel imager with enhanced chemiluminescence (ECL) detection (Thermo Fisher Scientific). Densitometric analysis of protein bands was performed in Fiji78.
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bioRxiv - Cell Biology 2022Quote: ... The supernatant containing solubilized mitochondria was supplemented with Coomassie Blue G-250 dye to a final detergent:dye ratio of 16 g/g before running on a 3-12% NativePAGE Mini Protein Gel (ThermoFisher) according to manufacturer’s directions ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were transferred to nitrocellulose with a semi-dry transfer system (Life Technologies Inc.: 65V/gel, 90 min). Membranes were blocked for 30 min with 5% milk in PBS containing 0.1% Tween 20 at 25 °C ...
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bioRxiv - Neuroscience 2021Quote: ... was used as a molecular mass marker and the gels were stained with Colloidal Blue Staining Kit (Invitrogen, Germany), as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 8% SDS and 2% β-mercaptoethanol) were loaded onto 12% SDS-polyacrylamide gel and See-Plus 2 (Invitrogen, LC5925) was used as a molecular-weight marker ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were precipitated with anti-Myc antibody and ran on a 4-12% NuPAGE Bis-Tris gel (ThermoFisher) and blotted using the MiniBlot Module (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... Eluates were collected from the beads by centrifugation and resolved on a NUPAGE 4-12 % Bis-Tris gel (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... and separated on 10% polyacrylamide gel alongside dual-color Page Ruler Prestained Protein size markers (Thermo Fisher Scientific, Poland). The separated proteins were then transferred onto a Westran S PVDF membrane (GE Healthcare ...
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bioRxiv - Genetics 2021Quote: ... for 10 minutes at 70°C before loading into Bolt 4-12% Bis-Tris Plus gels (Thermo Fisher Scientific). Gels were run at 120V for 2-3 hours in Bolt MES SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The harvested DNA was detected by the agarose gel electrophoresis and quantified by Qubit 3.0 (Thermo Fisher Scientific, USA). Whole-genome sequence determinations of strain CS1 were carried out with the PacBio (Pacific Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... 21.7μg C-1 and 7.9μg 10-1) of each sample was separated was separated on a 10% Bis-Tris Novex mini-gel (Invitrogen) using the MES buffer system ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μg of protein from extracts were separated in SDS–polyacrylamide gels 4–12% (NuPAGE Bis-Tris, Thermo Scientific) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of protein for each sample were loaded and run on a 4–12% SDS-PAGE gel (Invitrogen). A nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... iProtein extracts were resolved in NuPageTM 4-12 % BisTris Protein Gels (Cat No. NP0321BOX, Thermo Fisher Scientific, MA, US), and blotted onto an Invitrolon™ PVDF (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: HCT116 cell lysates were combined with Laemmli buffer and resolved on Novex WedgeWell 4-20% Tris-Glycine gels (Invitrogen). Gels were either stained with Novex SimplyBlue SafeStain (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... Approximately 30 μg of soluble supernatant was run on a 4-20% Tris-Gly SDS PAGE gel (Life Technologies) and transferred onto a nitrocellulose membrane using an iBlot2 device (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... Samples and Blue Native Protein Ladder (Thermo LC0725) were loaded onto NativePAGE Bis-Tris Gels (3-12%, Invitrogen BN1001BOX) and gel electrophoresis was performed using anode NativePAGE Running Buffer (Invitrogen BN2001 ...
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bioRxiv - Microbiology 2021Quote: ... The resuspended pellets were analyzed using Novex 4-20% SDS– SDS-PAGE mini gel (Thermo Fisher Scientific, Waltham, MA) to quantify the S trimer/rRBD copies ...
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bioRxiv - Microbiology 2021Quote: ... samples were not heated nor reduced before being run in a 4-12% acrylamide denaturing gel (NP0323, NuPAGE, ThermoFisher), and then transferred onto a nitrocellulose membrane (IB23001 ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates and virions pelleted through 20% sucrose (14,000xg for 90 minutes at 4°C) were separated on a NuPage 4-12% Bis-Tris Gel (Invitrogen) and subsequently blotted onto a nitrocellulose membrane ...
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bioRxiv - Immunology 2021Quote: ... were heated to 95°C for 5 min and 10 μl (2.5 μg) were loaded to a NuPAGE 10% Bis-Tris gel (Invitrogen). The gel was run in NuPAGE MOPS Buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA/protein complexes were then electrophoreticed by 6% native polyacrylamide gel and transferred to nylon membrane (Thermo Fisher Scientific). RNA was cross-linked with a UV lamp at a distance of 0.5 cm from the membrane for 2 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µg of this total RNA input was loaded onto a precast 10% TBU acrylamide urea gel (Novex / ThermoFisher) and electrophoresed at 120 V for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... 2010) as follows: protein samples were separated in NuPAGE™ 3-8% Tris-Acetate Protein Gels (Thermo Fisher EA03755) in Tris-Acetate-EDTA running buffer (Thermo Fisher BP13354 ...
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bioRxiv - Neuroscience 2022Quote: ... We loaded 25 μL of cell lysate solution into the wells of NuPAGE 10% Bis-Tris gels (Invitrogen; NP0301BOX) and run for 55 min at 200 V ...
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bioRxiv - Microbiology 2022Quote: ... soluble fractions were then separated under native conditions on a 4–16% NativePage Novex BisTris Protein Gel (Life Technologies) and transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μl of proteins obtained after TCA precipitation were separated by 4-12% polyacrylamide gradient gel electrophoresis (NuPAGE-Invitrogen) in 1X MES buffer (50 mM MES ...
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bioRxiv - Molecular Biology 2022Quote: ... the gels were either subjected to Northern blotting as described below or stained with SYBR™ Safe stain (Invitrogen).
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bioRxiv - Genomics 2022Quote: ... The RNA quality was checked on agarose gel and quantified using a NanoPhotometer spectrophotometer (Thermo Fisher Scientific, Wilmington, DE). An equal amount of RNA of each tissue was pool to generate three libraries per tissue using 4 individuals per library ...
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bioRxiv - Microbiology 2022Quote: ... at 90°C for 10 min before being run on a 4-12% acrylamide denaturing gel (ThermoFisher, #NP0323 NuPAGE). Proteins were then transferred onto a nitrocellulose membrane (ThermoFisher ...