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Citations for Thermo Fisher :
6851 - 6900 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... BN-PAGE gels were run for 2 to 2.5 h at 150 V using NativePAGETM running buffer (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were verified and quantified by agarose gel electrophoresis with a standard low DNA mass ladder (Invitrogen). Purification and OneShot Sanger sequencing of merA and merAB gene products was performed by Genoscreen (Lille ...
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bioRxiv - Systems Biology 2020Quote: ... DNA was checked for quality by gel electrophorese and by using a Qubit 4 Fluorometer (Thermo Fisher Scientific, USA). Metagenomic sequencing was performed by Novogene Ltd ...
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bioRxiv - Neuroscience 2021Quote: ... extracted nuclear proteins were subjected to NuPAGE Bis-Tris (4 to 12%) gel electrophoresis under reducing conditions (ThermoFisher Scientific) and then electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... Gels were run for 1 hour and 15 min at 120 V in with NuPage MES Buffer (NP0002, ThermoFisher). Proteins were then transferred onto polyvinylidene difluoride membrane (IPVH15150 ...
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bioRxiv - Biochemistry 2021Quote: Protein concentration was quantified by loading proteins on denaturing SDS-PAGE gel and stained with SYPRO Red following the manufacturer’s recommended protocol (ThermoFisher). Linear dynamic range of the quantification was established by separately staining a titration series of known amount of MBP-paramyosin-Sal ...
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bioRxiv - Biochemistry 2021Quote: ... PCR reactions were analyzed for purity on an agarose gel and purified using GeneJET PCR purification kit (Thermo Fisher).
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bioRxiv - Genetics 2021Quote: ... The libraries were subjected to size selection (250–600 bp) on a 4–20% TBE PAGE gel (Thermo Scientific). The TBE gel was run at 180V ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were separated on NuPAGE™ Novex® 4-12% Bis-Tris protein gels in MES buffer (Life Technologies) and were transferred onto nitrocellulose membranes using the iBlot® system (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... and run at 180 V for 50 min on a 10% acrylamide TBE-Urea Novex gel (Thermo Fisher Scientific). Gels were stained with 2 µg/mL ethidium bromide (Amresco ...
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bioRxiv - Bioengineering 2021Quote: ... DNA quality was assessed using a 2% agarose gel and purified using the GeneJet PCR kit (Thermo Fisher Scientific). DNA was then eluted to a concentration of between 25-100 ng/µL and amplified using the T7 MegaShortScript RNA synthesis kit (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: Full-length N was separated from the lower molecular weight proteoforms on a 0.8 mm 4-12% bis-tris SDS-PAGE gel (Invitrogen) and stained with Coomassie Blue ...
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bioRxiv - Biochemistry 2020Quote: ... samples were mixed with loading buffer and applied to a 4-16% Bis-Tris native gel (Thermo Fisher Scientific) together with NativeMark unstained protein standard (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the EMX1 sgRNA was confirmed by electrophoresis on a 12% TBE-UREA-PAGE gel (Thermo Scientific). Concentrations were determined by A260nm using a NanoDrop instrument (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... at 95 °C for 5-10 min and loaded on a gradient 4-12% NuPAGE Bis-Tris gels (Invitrogen). After electrophoresis at 200 V ...
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bioRxiv - Neuroscience 2021Quote: ... to measure total protein concentrations according to manufacturer’s instructions 25 µg of total proteins of all samples were loaded onto a 4-12% gradient gel (Invitrogen) and blotted onto a PVDF membrane (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... Samples ranging from 15-40 μg were loaded onto a NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The eluates were loaded on a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and analysed with IstantBlue staining (Merck).
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were extracted from the gel blocks in 30 % acetonitrile (Biosolve) and 0.5 % formic acid (Biosolve) and dried in a SpeedVac Vacuum Concentrator (ThermoFisher). Samples were analyzed by LC-MS/MS on a 20 cm x 75 µm C18 column (BEH C18 ...
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bioRxiv - Cell Biology 2021Quote: ... Mitochondrial respiratory supercomplexes were resolved with BN-PAGE using the 4–16% NativePAGE Novex Bis-Tris Mini Gels (Invitrogen) in a Bis-Tris/Tricine buffering system with cathode buffer initially supplemented with 0.02% G-250 followed by the 0.002% G-250 ...
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bioRxiv - Cell Biology 2021Quote: ... the insoluble pellet fractions (2 A260 units) were separated on NuPAGE 4-12% Bis-Tris 15-well gels (Invitrogen), run in NuPAGE 1x MOPS SDS running buffer (Novex) ...
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bioRxiv - Cell Biology 2021Quote: ... and insoluble pellet (0.5 A260 units) were separated on NuPAGE 4-12% Bis-Tris 26-well Midi gels (Invitrogen), run in NuPAGE 1x MES SDS running buffer (Novex).
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bioRxiv - Cancer Biology 2020Quote: ... The reverse cross-linked immuoprecipitated DNA fragments were purified using GeneJET gel extraction kit (Thermo Scientific, Waltham, MA, USA) followed by PCR analysis for the p53-responsive DNA elements on the human ARTS promoter using the following primers ...
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bioRxiv - Cancer Biology 2020Quote: ... and transferred onto nitrocellulose membranes with pre-cast gels via the iBlot-2 system (Thermo Fisher Scientific, Waltham, MA). The membrane was then incubated at room temperature for 1-2 hours in LICOR Odyssey blocking buffer and incubated at 4°C with the appropriate antibody overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... equal amounts of proteins across conditions were separated on NuPAGE Novex Bis-Tris 4–12% Midi gels (Life Technologies) at room temperature and constant voltage ...
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bioRxiv - Cell Biology 2021Quote: ... Each lane was divided into twelve fractions and the proteins were in-gel digested with trypsin (Thermo Fisher Scientific) at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2020Quote: All proteins were separated on pre-cast 4-12 % Bis-Tris SDS-PAGE gels (NPO321BOX, NPO322BOX, NPO336BOX) from Invitrogen using MES running buffer (50mM MES ...
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bioRxiv - Pathology 2021Quote: ... Fifteen micrograms of proteins were separated by Blue Native-PAGE Novex 4–16% Bis-Tris gel (Thermo Fisher Scientific). Gels were stained with Coomassie Brilliant Blue ...
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bioRxiv - Biochemistry 2021Quote: ... The gels were run at 4 °C using the electrophoresis system according to the Invitrogen instructions (ThermoFisher Scientific, USA), and then stained using the Pierce Silver Staining kit according to the instructions (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Equivalent protein amounts (20-40 ug) were run on 10% NuPAGE Bis-Tris gels (ThermoFisher Scientific, cat. no. NP0301) and transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were separated by electrophoresis under denaturing conditions on NuPage Bis-Tris Pre-Cast gels (Invitrogen, Carlsbad, CA) for 60 min at 200 Volts and transferred onto nitrocellulose membranes (Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... 600 mM DTT) was added to lysates before separation on 12% Novex Tris-glycine gels (Thermo Fisher Scientific, XP00120BOX). Separated proteins were transferred onto PVDF membranes (Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen, EC6265BOX). Bands of the appropriate size were excised ...
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bioRxiv - Cell Biology 2021Quote: ... Digitonin-solubilized mitochondria proteins (100 µg) were separated on pre-cast 3%-12% gradient blue native gels (Invitrogen #BN1001) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplicons were visualized with SYBR Safe™ DNA Gel Stain in 2% Ultra Pure Agarose (Thermo Scientific, USA). DNA bands were excised and purified using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... and RNA integrity was assessed with gel-electrophoresis and quantified using a Nanodrop (Thermo Fisher Science, Waltham, MA, USA). Library were prepared and singleend 50 bp sequenced on the DNBseq platform at BGI (Hong Kong ...
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bioRxiv - Microbiology 2021Quote: ... After analysis through electrophoresis on a 1% agarose gel as well as a Qubit 2.0 (Thermo Scientific, Waltham, USA), a NEBNext Ultra™ ‖ DNA Library Prep Kit (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... Polyacrylamide gels (10%) were blotted by dry transfer on 0.2μM nitrocellulose membranes using the iBlot™ 2 Gel Transfer Device (ThermoFisher). Primary anti-alpha-synuclein (1:1000 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... stained for 20 min in SYBR™ Green II RNA Gel Stain (1:10,000 in 0.5x TBE, ThermoFisher Scientific) and imaged on the iBright™ (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... Extracted DNA was visualized on 2.0% agarose gels and quantified with a Qubit 2.0 fluorometer (Thermo Fisher Scientific, Inc.). Library preparation followed a double digest Restriction-Site Associated DNA (ddRAD ...
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bioRxiv - Immunology 2020Quote: ... One million cells from lung cell suspension were resolved on 4 – 12 % NuPAGE Bis-Tris protein gels (Thermo Fisher), then transferred onto a nitrocellulose membrane (Biorad ...
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bioRxiv - Immunology 2020Quote: ... following SDS-PAGE in reducing and non-reducing conditions using NuPAGE™ 3-8% Tris-Acetate gels (Life Technologies). Purified proteins were dialyzed overnight against PBS using Slide-A-Lyzer® dialysis cassettes (10 kDa MW cut-off ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein (15 μg) for each sample were loaded into 4-12% gradient Bis-Tris gels (Invitrogen) and separated by electrophoresis ...
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bioRxiv - Physiology 2021Quote: ... Gel-purified RT-PCR products were then cloned into pCR4-TOPO vector using the TOPO TA cloning kit (Invitrogen), and automated DNA sequencing of cloned PCR products was performed using Big Dye chemistry (ABI 3730 capillary sequencer ...
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bioRxiv - Systems Biology 2020Quote: ... 16µl of sample were loaded into 1.0mm 15 well pre cast Novex 4-20% Tris-Glycine Gels (Invitrogen #XP04205BOX) and 30µl into 10 well pre cast gels ...
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bioRxiv - Microbiology 2021Quote: ... The obtained PCR products were purified using a GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Waltham, MA, United States). Sequencing libraries were generated following manufacturer’s recommendations with NEB Next® Ultra™ DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... 30 μg total protein was loaded in each well and electrophoresed on the sodium dodecyl sulfate–polyacrylamide gel (ThermoFisher) and transferred to the PVDF membrane ...
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bioRxiv - Microbiology 2020Quote: ... Protein quality and quantities were checked by SDS-PAGE electrophoresis (12% polyacrylamide gels) and UV spectrometry (Nanodrop, Thermo Scientific). A total of 100 μg protein from each condition was subjected to in-solution trypsin digestion before labelling the resultant tryptic peptides using the TMT-6plex Isobaric Label Reagent Set (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... DNA quality and concentration were evaluated using a 0.8% agarose gel and the Quant-iT PicoGreen dsDNA assay kit (Thermofisher). Subsequent to the quality check ...
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bioRxiv - Cancer Biology 2020Quote: ... Amplified DNA of target gene was mixed with 6x DNA gel loading dye (Thermo Fisher Scientific, Cat No. R0611) and subjected to gel electrophoresis at 105 V for 30 min ...