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Citations for Thermo Fisher :
6801 - 6850 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Samples were loaded onto the gel and run with 1X Bolt™ MES SDS Running Buffer (Thermo Fisher Scientific) in the Mini Gel Tank (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... 10 μg of protein were loaded onto a 15-well NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen), run at 100 V for 2.5 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... The libraries were subjected to size selection (250–500 bp) on a 4–20% TBE PAGE gel (Thermo Scientific). The recovered libraries were sequenced on the Illumina MiniSeq platform using the MiniSeq High Output kit with 2×150 paired-end.
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bioRxiv - Microbiology 2020Quote: ... then heated to 42°C for 15 min before loading to 12% Novex Tris-Glycine SDS-PAGE gels (ThermoFisher). The eluted protein was resolved by electrophoresis at 150 V for 60 min ...
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bioRxiv - Immunology 2021Quote: ... 10ug of protein per sample was resolved on NuPage 4-12% Bis-Tris precast gels in MES buffer (Invitrogen), transferred to 0.45μm PVDF membrane then blocked with 5% BSA in TBS supplemented with 0.1% Tween-20 ...
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bioRxiv - Microbiology 2019Quote: ... Blue native electrophoresis of the concentrated complexes was carried out on a 3-12% Bis-Tris gel (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the extracted RNA samples were run on standard RNase-free 1.0% agarose gels and measured using a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 150 µg of protein extract from each sample were ran on NuPAGE 4%–12% Bis–Tris Protein Gels (Invitrogen) using MES running buffer (100 mM MES ...
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bioRxiv - Molecular Biology 2020Quote: ... gel-purified RNA fragments of around 17-30 nucleotides were treated with 10 units of T4 PNK (Thermo Fisher) in a low-pH buffer (700 mM Tris ...
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bioRxiv - Neuroscience 2019Quote: ... 25µg of total protein (60µg for PLCγ1) in 20µL was run on 4-20% tris-glycine gels (ThermoFisher #XP04205BOX, USA) for 120 minutes at 130V ...
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bioRxiv - Molecular Biology 2020Quote: ... were resolved on precast NuPAGE 4-12% Bis-Tris midi 12+2-well protein gels (cat. no. WG1401BOX, Invitrogen) at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Molecular Biology 2020Quote: ... ∼50 µg of protein extracts was then resolved on precast NuPAGE Novex 4–12% Bis-Tris gels (Invitrogen, NP0321BOX). The proteins were transferred to a nylon membrane with the semidry transfer Pierce Power System (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA loading buffer was added and 10 mL of the reactions were electrophoresed on 6% DNA Retardation gels (Invitrogen) at 130 V for 60 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The libraries were selected from 250–350-bp fragments obtained by E-Gel 2% SizeSelect electrophoresis (Invitrogen, cat# G661012). Size-selected libraries were verified using the Agilent 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... The gel was washed three times with distilled water (diH2O) and stained with GelCode Blue Protein Safe stain (Invitrogen). In order to confirm and evaluate Rep and Cap expression from the new Rep hybrid plasmids Western-blot analyses were performed ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were then subjected to SDS gel electrophoresis before being transferred to nitrocellulose membranes using iBlot2 (Life Technologies), blocked with 5% BSA in TBS with 0.1% Tween-20 and incubated with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... or 10-20% Tris-glycine gel (for LC3) and then electro-transferred to a 0.45 μm (or 0.2 μm) PVDF membrane (Thermo Scientific). After blocking with 5% milk ...
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bioRxiv - Genomics 2020Quote: ... 28 mM TASP, 0.08 mM EDTA, pH 8.7) (Sage Science, USA) containing SYBR Safe DNA gel staining (Invitrogen, USA).
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bioRxiv - Genomics 2020Quote: ... The integrity and purity of the RNA were determined by gel electrophoresis and a NanoDrop ND-1000 (Thermo Scientific), respectively ...
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bioRxiv - Immunology 2021Quote: ... protein lysates were resolved in a 10% agarose gel with SDS and transferred to a nitrocellulose membrane (Fisher Scientific). Membranes were blocked in 5% bovine serum albumin v (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... An equal amount of protein per sample was loaded into 2-12% gradient NuPage Bis-Tris gels (Thermo Scientific) and protein was separated by electrophoresis ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions were incubated on ice for 20 min and separated on a precast Bis-Tris Native gradient Gel (Invitrogen) in 1x running buffer (50 mM Bis-Tris 50 mM Tricine pH 6.8 ...
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bioRxiv - Cancer Biology 2020Quote: ... The annealed product was gel purified and cloned into a pJet vector using the CloneJET PCR Cloning Kit (Thermofisher). The vector was used to transform chemically-competent E ...
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bioRxiv - Microbiology 2019Quote: ... sonicated and denatured at 95°C for 10 min and then separated on Tris-Glycine-SDS polyacrylamide gels (Invitrogen). After electrophoresis ...
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bioRxiv - Cell Biology 2019Quote: Between 40-50µg of samples were run on NuPAGE Bis-Tris 4–12% gels in NuPage MES SDS Buffer (Invitrogen) for 40 minutes at 200 V and transferred to 0.4-µm nitrocellulose membranes using a semi-dry transfer system (Bio-Rad Catalog #1704150 ...
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bioRxiv - Cell Biology 2019Quote: ... Five μg of proteins were loaded onto a 4-12% (wt/vol) NuPAGE Bis-Tris gel (Thermo Fisher Scientific), and SDS-PAGE and immunoblotting were conducted following standard protocols ...
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bioRxiv - Genomics 2020Quote: ... The samples were then loaded onto a NuPAGE 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific Cat# NP0335BOX) and a short gel was run using NuPAGE MOPS SDS Running Buffer (Thermo Fisher Scientific Cat# NP0001 ...
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bioRxiv - Synthetic Biology 2019Quote: ... 20 μg of soluble protein per sample were run in a 4-20% Tris-Glycine gradient polyacrylamide gel (Invitrogen) and transferred to a ProTran NitroCellulose transfer membrane (Whatman ...
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bioRxiv - Neuroscience 2019Quote: ... A molecular mass standard was run on each gel (Novex Sharp Pre-stained Protein Standard, Life Technologies, Carlsbad, CA). Membranes were blocked for one hour at room temperature and probed with primary antibodies using conditions optimized to be within the linear range of detection for each primary antibody (Table 2) ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were verified and quantified by agarose gel electrophoresis with a standard low DNA mass ladder (Invitrogen). Purification and OneShot Sanger sequencing of 16S rRNA gene products was performed by Genoscreen (Lille ...
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bioRxiv - Synthetic Biology 2019Quote: ... The gels were washed with deionised water three times (5 minutes each) and stained with Coomassie blue (SimplyBlueStain, Invitrogen) overnight and destained with deionised water for 4-6 hrs ...
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bioRxiv - Biochemistry 2019Quote: ... 0.25 M cell equivalents were run on each lane of a 7% NuPage Tris-Acetate gel (Invitrogen, Carlsbad, CA) and then transferred to an Immobilon-FL PVDF membrane (EMD Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Gel pieces were incubated in Elution buffer (1% formic acid, 2% acetonitrile in LC-MS grade water (Thermo Scientific)) and dried in a SpeedVac ...
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bioRxiv - Genomics 2019Quote: ... The amplified Tn5 libraries were then size selected from 300 - 800 bp on a 2% EX E-gel (Thermofisher) and purified using QIAquick gel extraction kit (Qiagen) ...
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bioRxiv - Biochemistry 2019Quote: ... Gels were stained in 0.5x TBE buffer containing a 1:10000 dilution of Sybr Gold Stain (Thermo Fisher Scientific), for 30 min with shaking ...
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bioRxiv - Immunology 2019Quote: ... loaded onto NuPAGE® 4-12% gels and run at 120 V in 1 X MES buffer (ThermoFisher Scientific). The separated proteins were transferred onto membranes using the iBlot® transfer system (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated using SDS–PAGE with Novex 4–20% Tris-glycine polyacrylamide gel (Invitrogen, Life Technologies, Carlsbad, CA). Western blot analysis was performed using peroxide-conjugated anti-hemaglutinin antibody at 1:250 dilution (Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated using SDS–PAGE with Novex 4–20% Tris-glycine polyacrylamide gel (Invitrogen, Life Technologies, Carlsbad, CA). Western blot analysis was performed using peroxide-conjugated anti-hemaglutinin antibody at 1:250 dilution (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... heated at 70°C for 10min and ran on a 7cm NuPAGE Novex 10% Bis-Tris gel (Life Technologies) at 200 V for 6min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Amplicons were visualized on 0.8% agarose gel electrophoresis using 1× TAE buffer stained with SYBRSafe (Invitrogen, Carlsbad CA, USA), and compared to a GeneRuler 1 Kb Ladder (ThermoScientific ...
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bioRxiv - Developmental Biology 2019Quote: ... chilled on ice for 5 min and electrophoresed in a 3 – 8% Tris-Acetate NuPAGE gel (Novex Life Technologies). Transfer of the proteins onto a nitrocellulose membrane was done in transfer buffer (5% methanol ...
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bioRxiv - Physiology 2019Quote: ... 25 µg or 50 µg of ventricular mouse heart lysate samples were loaded on a 1.0 mm-thick 10% Tris-acetate gel for high molecular weight proteins (Invitrogen). Gels were run at 150 V for 1 hour and then subsequently transferred to nitrocellulose membranes using the Biorad Turbo Transfer System (Biorad ...
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bioRxiv - Cell Biology 2019Quote: ... boiled for 5 min at 90°C and loaded on NuPAGE Novex 4–12% Bis-Tris Protein Gels (Invitrogen). Gels were then fixed for 30 min at RT in fixing solution (40% [v/v] methanol ...
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bioRxiv - Genetics 2020Quote: ... Quality/quantity of the extracted DNA was evaluated by gel electrophoresis and Qubit dsDNA BR Assay Kit (Life Technologies). To evaluate the probability of DNA degradation ...
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bioRxiv - Genetics 2020Quote: ... Samples were then crosslinked with 0.2% glutaraldehyde (37°C, 5 min) then run on a 0.8% agarose 1xTAE gel and stained with SYBR Gold (ThermoFisher).
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bioRxiv - Cancer Biology 2021Quote: ... 25 μg of protein per lane were resolved on NuPAGE Novex Bis-Tris 4-12% polyacrylamide gels (Invitrogen, #NP0321BOX) and transferred onto nitrocellulose blotting membranes (Amersham Protran ...
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bioRxiv - Immunology 2021Quote: ... proteins with a molecular weight (MW) below 100 kDa were separated on 4-12% Bis/Tris gels (Life Technologies) in MES SDS Buffer (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and proteins with a molecular weight above 100 kDa were separated on 3-8% Tris/Acetate gels (Life Technologies) in MOPS Buffer (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Samples were spun to remove any debris and loaded onto a 4-12% Bis-Tris NuPAGE gel (Thermo Fisher) and separated for 3 hours at 4C at 100V ...
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bioRxiv - Neuroscience 2020Quote: ... The sample was digested with nuclease and electrophoresed on Novex TBE Gels (10%, 12 well; Thermo Fisher Scientific, EC62752BOX) and then subjected to nucleic acid staining with GelRed (Fujifilm Wako Chemicals ...