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Citations for Thermo Fisher :
6551 - 6600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... and 1 U/μl Superase-in RNAse inhibitor (AM2694 ThermoFisher) (Cutting Buffer) ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted using a TRIzol kit (Invitrogen, Carlsbad, CA, USA), and high quality RNA samples detected by the Agilent2100 Bioanalyzer instrument were subjected to PE150 sequencing using a BGISEQ500 platform (BGI ...
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bioRxiv - Plant Biology 2024Quote: ... 50 μl packed volume of each life-stage were washed in 10 mM MES buffered (pH 6.5) water and resuspended in 100 μl of ice cold 3xHank’s Balanced Salts Solution (14065-056 Gibco-BRL), supplemented with 2% Foetal Bovine Serum (A3160601 Gibco-BRL ...
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bioRxiv - Plant Biology 2024Quote: ... Luminescence was measured using a Fluoroskan Ascent FL microplate fluorometer and luminometer (Thermo Scientific). ROS production was reported as either the increase in photon counts or the sum of total photon counts.
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bioRxiv - Synthetic Biology 2024Quote: The Hek293T cell line (ATCC) was cultured in DMEM Gluta-max (Gibco) supplemented with 10% Tet-Free Fetal Bovine Serum (Euroclone ...
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bioRxiv - Plant Biology 2024Quote: ... supplemented with 2% Foetal Bovine Serum (A3160601 Gibco-BRL) and 1 U/μl Superase-in RNAse inhibitor (AM2694 ThermoFisher ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was run using a QuantStudio5 Real-Time PCR System (Applied Biosystems) and by using the PowerUp SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Goat anti-rabbit IgG AlexaFluor 633 (Invitrogen, A21070).
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bioRxiv - Cancer Biology 2024Quote: CellROX green or CellROX orange (ThermoFisher) was applied to live cells cultured on glass bottom dishes (Cellvis ...
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bioRxiv - Cancer Biology 2024Quote: ... c-Myc, and SIRT2 (ON-TARGET plus SMART pool siRNA, Dharmacon) were used with Lipofectamine RNAiMAX Transfection Reagent (#13778075, ThermoFisher). Knock-down and knock-in were confirmed by western blot.
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bioRxiv - Cancer Biology 2024Quote: ... and DNA was extracted using Genomic DNA mini kit (#K182001, Invitrogen).
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bioRxiv - Physiology 2024Quote: ... A ∼200mg sample of muscle was collected using a sterile 6mm hollow Bergstrom biopsy needle (Pelomi, Albertslund, Denmark) modified for suction 28and placed in holding media for transport to the laboratory (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... Tim-4 (1:1000 dilution, rabbit anti-TIM-4 antibody, Invitrogen, PA5-116045, RRID:AB_2900679) and Lumican (1:1000 dilution ...
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bioRxiv - Plant Biology 2024Quote: ... and transferred to vials (ThermoFisher Scientific) for UHPLC-MS analysis.
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bioRxiv - Plant Biology 2024Quote: ... RNA quantity and purity were evaluated with a Qubit®2.0 fluorometer (Invitrogen™, Carlsbad, CA, USA) using a Qubit™ RNA BR assay kit ...
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bioRxiv - Plant Biology 2024Quote: ... the supernatant was loaded onto a column containing 1 mL of Ni-NTA resin (Invitrogen, Waltham, MA) that had been previously equilibrated with 1 column volume of lysis buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Isopropyl β-d-1-thiogalactopyranoside (IPTG, ThermoFisher Scientific) was added to a final concentration of 1 mM to induce protein expression ...
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bioRxiv - Synthetic Biology 2024Quote: ... and mounted in 50 µL of Prolong Gold (ThermoFisher #P10144) before imaging.
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bioRxiv - Synthetic Biology 2024Quote: ... Donkey anti-rabbit IgG AlexaFluor 555 (Invitrogen, A32794), Goat anti-rabbit IgG AlexaFluor 633 (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... and by using the PowerUp SYBR Green Master Mix (Applied Biosystems), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNA was treated with Turbo DNase (ThermoFisher #AM2238) and purified using the RNeasy kit RNA cleanup protocol ...
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bioRxiv - Synthetic Biology 2024Quote: Cells in a 24-well plate were trypsinized with 50 μL of 0.25% Trypsin (ThermoFisher #25200056) for 5 minutes at 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 400 μL of n-hexane (Acros Organics; 160780010) in a capped mass spec autosampler vial (Microliter ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to assess sample quality and cDNA concentrations were quantified using a Qubit 2.0 Fluorometer with the dsDNA-High Sensitivity kit (Life Technologies Inc., Carlsbad, CA), yielding mean cDNA concentration of 1ng/ul ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA oligos (Table S2) were ordered to IDT (Coralville, Iowa, USA) and polymerase enzymes used for DNA amplification were Phusion (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were resuspended in PBS (pH 7.4, Gibco) and then subjected to five freeze and thaw cycles by alternating between liquid nitrogen and a 37 °C water bath ...
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bioRxiv - Synthetic Biology 2024Quote: ... glass bottom plates were coated overnight by diluting human laminin (ThermoFisher #A29249) to a surface density of 0.5 µg/cm2 ...
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bioRxiv - Neuroscience 2024Quote: ... glass coverslips were then mounted on glass slides using Prolong Gold antifade reagent (Thermofisher) and imaged by confocal microscopy ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA samples were assessed for concentration using a Qubit fluorometer (ThermoFisher Scientific, Waltham, MA) and quality using the 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... to visualize nuclei and/or CellMask (ThermoFisher Scientific, Waltham, MA) to visualize the plasma membranes of cells as per product protocols ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor conjugated secondary antibodies (anti-mouse Alexa Fluor 488 and 568, anti-rabbit Alexa Fluor 488 and 568; ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2024Quote: ... The pellet collected was centrifuged 200g for one minute and fixed with 2.5% glutaraldehyde (GA) in sodium cacodylate buffer (SCB, ThermoFisher Scientific, Waltham, MA) for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... with 1µg of Plasmid DNA in 30µl of Opti- MEM medium (ThermoFisher). The mix was added to cultured neurons for 3 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were either frozen for whole brain sectioning/western blotting/qPCR or kept in RPMI (1X) + GlutaMAX (Gibco) + 1X MEM NAA for isolation of BBB-ECs ...
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bioRxiv - Physiology 2024Quote: Peptides were acidified by Trifluoroacetic acid (TFA) (Thermo Fisher Scientific, #FSBA116) to a final concentration of 1% TFA and centrifuged at 16,000 g for 10 minutes at room temperature ...
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bioRxiv - Physiology 2024Quote: ... then resuspended in digestion buffer (10% trifluoroethanol (Thermo Fisher Scientific, #96924) in 100 mM HEPES pH 7.5) ...
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bioRxiv - Physiology 2024Quote: ... SpCas9 mRNA and sgRNAs were purified using a MEGAclear kit (Life Technologies). The injection mix consisted of 100 ng/ml spCas9 mRNA and 50 ng/ml each sgRNA ...
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bioRxiv - Physiology 2024Quote: ... TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR) were then performed using a QuantStudio 7 Pro Real-Time PCR System (ThermoFisher). The housekeeping gene Vinculin was used as an internal control for cDNA quantification and normalization of gene amplified products.
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bioRxiv - Physiology 2024Quote: ... Samples were mounted using Fluoromount-G with DAPI (00-4959-52, Invitrogen), and images were collected using a Leica TCS SP8 microscope.
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bioRxiv - Neuroscience 2024Quote: ... 20 mM HEPES (1 M, #15630080, Gibco), and 25 mM Glucose (2.5 M ...
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bioRxiv - Physiology 2024Quote: ... The cardiomyocytes were finally plated onto laminin-coated coverslips (5 μg/ml, Thermo Scientific) and cultured in the M-199 medium supplemented with bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: HEK-293T cells were transfected after reaching 70% confluency via Lipofectamine 3000 (Thermo Fisher Scientific) (2.5 μg total cDNA ...
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bioRxiv - Physiology 2024Quote: ... The blocks were then treated with 1 mg/ml collagenase type II in Leibovitz’s L-15 Medium (Thermo Fisher Scientific Inc.) at 37°C for 30-45 minutes ...
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bioRxiv - Physiology 2024Quote: ... These cells were generated by transfecting HEK293 cells using Lipofectamine 2000 (Invitrogen; 2 μl per well) with D1ER cDNA (0.5 μg/well ...
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bioRxiv - Physiology 2024Quote: ... cells were transfected using Lipofectamine 2000 (Invitrogen; 2 μl per well) with cDNA (0.5 μg/well ...
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bioRxiv - Physiology 2024Quote: ... 1000 ng of total RNA was converted to cDNA using the “High-Capacity cDNA Reverse Transcription Kit” (Thermo Fisher Scientific, Ref# 4368814). Sample cDNA was diluted 1:10 before input for real-time Q-PCR reaction ...
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bioRxiv - Physiology 2024Quote: ... and γ subunits and mouse prostasin were transcribed using the mMessage mMachine transcription kit (Life Technologies). Resulting cRNA encoding each of the subunits was injected into stage V to VI Xenopus oocytes ...
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bioRxiv - Physiology 2024Quote: ... transferred to “Phasemaker tubes” (ThermoFisher Scientific, A33248), mixed with 140 μl of chloroform ...
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bioRxiv - Physiology 2024Quote: ... RNA quality and concentration were determined using the “Qubit RNA High Sensitivity Assay Kit” (ThermoFisher Scientific, Ref# Q32852), and a nanodrop spectrophotometer.
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bioRxiv - Physiology 2024Quote: ... only 4 wells of cells were incubated in DMEM with 4 μM fura-2/AM (fura-2/AM, Molecular Probes, USA) at 37° C in the dark for 45 min ...