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Citations for Thermo Fisher :
6751 - 6800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS) and antibiotics (Gibco). All cell lines were authenticated by short tandem repeat profiling and tested for mycoplasma contamination ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 100 U per mL penicillin/100 μg per mL streptomycin (Gibco).
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bioRxiv - Synthetic Biology 2024Quote: ... and finally mounted in Prolong Gold (ThermoFisher #P10144).
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bioRxiv - Synthetic Biology 2024Quote: ... The metabolite extract (100 μL) was added to 100 μL of 100 mM borate buffer (pH 10) (Thermo Fisher, 28341), 400 μL of 100 mM pentafluorobenzyl bromide (Millipore Sigma ...
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bioRxiv - Synthetic Biology 2024Quote: ... were washed three times in cold flow buffer and then the secondary antibody (Goat anti-Human IgG H+L Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488, ThermoFisher Scientific) at a dilution of 1:500 was added ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then filtered through a 40 μm cell strainer (Fisher Scientific #08-771-1) into a 96-well round bottom plate (Fisher Scientific #08-772-17) ...
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bioRxiv - Synthetic Biology 2024Quote: ... either Falcon Polystyrene Microplates (Fisher Scientific #08-772-1) for flow cytometry or MatTek No 1.5 uncoated glass bottom plates (Fisher Scientific #NC9988706 ...
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bioRxiv - Cancer Biology 2024Quote: ... using Gateway technology (Invitrogen, Thermo Fisher, US), followed by validation by Sanger sequencing and restriction digest ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in Hoeschst 33342 (62249, Thermo Scientific) and incubated at 37°C for 2hrs with slight agitation ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... Quantitative real-time PCR was performed using Applied Biosystems PowerUp SYBR Green Master Mix (Fisher Scientific, Cat#A25742) on QuantStudio6 real-time PCR system ...
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bioRxiv - Zoology 2024Quote: ... with optimized primers designed using Geneious Pro Bioinformatics Software (Supplement Table S1) on a StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, United States) using PowerUPTM SYBR® Green Master Mix (Applied Biosystems ...
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bioRxiv - Zoology 2024Quote: ... Tissue samples were subsequently rinsed three times with DPBS again and then transferred onto microscope slides in mounting buffer containing 1:1 DPBS: glycerol and 1µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Molecular Probes, Eugene, OR) to stain cell nuclei in prepared tissues and examined under a Lumen Dynamics XCite™ 120Q Nikon fluorescence microscope (Nikon ...
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bioRxiv - Zoology 2024Quote: ... ExoSAP-IT (Applied Biosystems) was used to clean PCRs ...
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bioRxiv - Zoology 2024Quote: ... using Lipofectamine 3000 or LTX reagent (Invitrogen, Burlington, ON) following the manufacture guidelines ...
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bioRxiv - Zoology 2024Quote: ... goat anti-rabbit Alexa 568 (Life Technologies, Burlington, ON) that was diluted 1:200 in DPBS containing 10% NSS ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... B27 (Life Technologies, Cat#: 17504-044), N2(Life Technologies ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... NPCs and dCas9-NPCs were cultured in DMEM/F12 with sodium pyruvate GlutaMAX medium (Thermo Fisher Scientific, Cat#: 10565018) supplemented with FGF2 (Bio-Techne ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... and penicillin-streptomycin (Thermo Fisher Scientific, Cat#: 15-140-122). NPCs were induced into neurons with doxycycline-inducible transcription factor NGN2 with neomycin antibiotic selection (Addgene #99378) ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... N2(Life Technologies, Cat#: 17502-048), and penicillin-streptomycin (Thermo Fisher Scientific ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... they were transplanted onto 12mm glass acid-etched coverslips coated in 80ug/mL Matrigel (Thermo Fisher Scientific, Cat#: 08-774-552) and cultured for a week to achieve appropriate coverage and attachment.
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... Starting at Day 2 post-induction iNs were cultured in Neurobasal medium (Life Technologies, Cat#: 21103-049) supplemented with N2 and penicillin-streptomycin ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... and separated on a BOLTTM 4-12% Bis-Tris 1mm gel (Thermo Fisher Scientific, Invitrogen, Cat#: NW04125BOX) at 200mV for 20-30 minutes ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... After 4-5 minutes 30 µM SCH-23390 (Fisher Scientific Cat#: 092550) in ACSF was applied for 1 minute ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... Osmolarity was measured at 290-300 mOsm with VAPRO® Vapor Pressure Osmometer (Fisher Scientific, ELITechGroup Model 5600 ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... The lysate was transferred to a clear flat-bottom 96-well plate to measure absorbance at 595 nm using a Varioskan plate reader (Thermo Scientific, Cat#: VL0000D0) and SkanIt Software for Microplate Readers ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... and 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat#: 15-140-122) was introduced through half medium changes every other day to promote electrophysiological maturation.
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... B27 Plus culture system medium (Thermo Fisher Scientific, Cat#: A36534-01) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Zoology 2024Quote: ... The HPLC was coupled to a Q-Exactive Plus (Thermo Scientific, Bremen, Germany) mass spectrometer ...
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bioRxiv - Zoology 2024Quote: ... on a 7500 Real-Time RT-PCR System (Applied Biosystems). Specific primers and probes (Table S1 ...
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bioRxiv - Biophysics 2024Quote: ... Real time PCR were carried out on the 7500 Real-Time PCR System (Thermo Fisher), using pre-PCR step of 20 s at 95 °C ...
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bioRxiv - Zoology 2024Quote: ... filtered (filter exclusion size 0.45µm, Fisher Scientific, France) and analyzed by RT-PCR and plaque assay.
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bioRxiv - Zoology 2024Quote: ... The amplicon DNAs were further analyzed for Sanger sequencing with the QuantumDye Terminator Cycle Sequencing Kit (Tomy Digital Biology) and specific primers (Table S1) upon ABI 3500 Genetic Analyzer (Applied Biosystems). The genome sequence of the M segment-coding region has been deposited in GenBank (accession numbers ...
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bioRxiv - Zoology 2024Quote: ... Cell densities of resuspended pellets were calculated using OD600 values measured on a Nanodrop 8000 (ThermoFisher Scientific). Cell densities were adjusted to produce 108 cells/ml for all strains ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... 6.0.2.3 (Thermo Scientific, Cat#: 5187139). Absorbance values were applied as a scaling factor for raw oxygen consumption rate (pmol/min ...
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bioRxiv - Neuroscience 2024Quote: ... followed by analysis with GeneMapper 4.0 software (Applied Biosystems) and conversion of peak size to GGGGCC repeat length via the calibration curve method according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... To precipitate the RNA, 250 μL of 2-propanol (AppliChem, Darmstadt, Germany) and 2 μL of GlycoBlue Coprecipitant (15 mg/mL) (Thermo Fisher Scientific, Waltham, MA, USA) were added to each sample ...
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bioRxiv - Neuroscience 2024Quote: ... Capillary electrophoresis was carried out on an ABI 3730 DNA Analyzer (Applied Biosystems, Waltham, MA. USA) using a ROX 1000 Size Ladder (Asuragen) ...
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bioRxiv - Neuroscience 2024Quote: NanoLC–MS/MS analyses were conducted using a nanoAcquity UPLC system (Waters) coupled with a Q-Exactive HF-X Mass Spectrometer (Thermo Scientific) equipped with a Nanospray Flex ion source ...
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bioRxiv - Neuroscience 2024Quote: ... respectively Alexa Fluor 488 (Invitrogen, Waltham ...
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bioRxiv - Zoology 2024Quote: ... PCR products were purified using ExoSAP-IT reagent (ThermoFisher Scientific) and sequenced by Macrogen Spain Inc.
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bioRxiv - Zoology 2024Quote: Media containing pooled excreta was filtered through 0.22 µm filter (Milex-GV®, Fisher Scientific, France) and 150 µl of the filtrate were combined with 350 µl of DMEM to inoculate T25 flasks containing 8.5 x 105 Vero cells for 1h15 at 37°C ...
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bioRxiv - Zoology 2024Quote: ... psittaci DNA control and UltraPure DNase/RNase-free distilled water (Invitrogen), respectively ...
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bioRxiv - Zoology 2024Quote: ... and the slides were reacted with FITC-conjugated goat anti-feline IgG (H + L) antibody (Thermo Fisher Scientific) diluted with PBS at a ratio of 1:500 for serum specimens and the negative control ...
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bioRxiv - Zoology 2024Quote: Seven and eight pupae were separately incubated with 300 µl of pooled excreta solution in one well of 48-well flat-bottom plate (Falcon™, Fisher Scientific, France). Pupae were placed in a climatic chamber with rearing conditions ...
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bioRxiv - Biophysics 2024Quote: ... in a reaction volume of 10 µl containing 5 µl of TaqMan® Fast Advance Master Mix and 0.5 µl of TaqMan gene expression assay 20X (Thermofisher). The entire process (retrotranscription ...
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bioRxiv - Zoology 2024Quote: ... Insect cells medium was also supplemented with 1 % non-essential amino acid (Gibco, France). Mosquito cells were grown at 28°C and mammalian cells at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... CD44 (Hs00153304_m1) and the housekeeping genes GAPDH (Hs99999905_m1) and GusB (Hs99999908_m1) were from Thermofisher. Real time PCR were carried out on the 7500 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Zoology 2024Quote: ... The online oligo design tools from ThermoFisher Scientific were used to calculate the GC content and melting temperatures for the designed primers ...
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bioRxiv - Zoology 2024Quote: ... cell suspensions of each microalgae strain were aliquoted into 24-well plates (ThermoFisher Scientific 930186 BioLite 6 well multidish ...
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bioRxiv - Zoology 2024Quote: ... Sanger sequencing was performed using ABI 3730XL DNA Analyser with BigDyeTM Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific), and the sequences were submitted to NCBI GenBank.