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Citations for Thermo Fisher :
6651 - 6700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Visualizations of the primary antibodies were achieved using suitable secondary antibodies conjugated with Alexa fluorophores (Alexa Fluor 488, 594, and 647, Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... All constructs were prepared by Genscript USA (Piscataway, NJ) and cloned into a pcDNA3.1(+) vector backbone (Thermo Fisher Scientific, Waltham, MA). Constructs were confirmed by sequencing analysis.
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bioRxiv - Neuroscience 2024Quote: ... in phenol red-free Opti-MEM I reduced serum medium (Gibco/Thermo Fisher Scientific) (2 h ...
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bioRxiv - Developmental Biology 2024Quote: ... washed 3 times with PBT1% (1% Triton X-100 in PBS) and permeabilised using a 0.25% trypsin solution (Gibco) at 25°C when embryos were older than 24 hpf ...
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bioRxiv - Developmental Biology 2024Quote: ... and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix ...
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bioRxiv - Developmental Biology 2024Quote: ... Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScript™ III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, France) from 200 ng of katna1+/+ and katna1-/-MZ RNA extracts using the following primers:
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bioRxiv - Developmental Biology 2024Quote: ... and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were used: Zn-5 (1/200; ZIRC, University of Oregon) and GFP (1/1000; Molecular Probes). After several washes in PBT1% ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were incubated overnight at 4°C with the appropriate secondary antibody (Alexa Fluor 488 or 555 at 1/1000, Molecular Probes).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using bicinchoninic acid assay (BCA) assay (#23225, Thermo Fisher Scientific). Equal amounts of protein were separated by 4-12% NuPAGE (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and 200 µg/ml hygromycin (Life Technologies, Grand Island, NY) (37°C ...
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bioRxiv - Neuroscience 2024Quote: ... cells were harvested using pre-mixed Earle’s Balanced Salt Solution (EBSS) with 5 mM EDTA (Life Technologies) and centrifuged at 3000 rpm (10 min ...
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bioRxiv - Neuroscience 2024Quote: ... and an autosampler coupled with a TSQ Vantage mass spectrometer (Thermo Fisher Scientific). Separation of the analyte from potentially interfering material was achieved at ambient temperature using an Agilent Eclipse Plus column (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were passaged with Versene (ThermoFisher Scientific, Waltham, MA), and transferred onto fresh Matrigel (Corning ...
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bioRxiv - Neuroscience 2024Quote: ... they were loaded with 2 µM Fura-2-AM (Invitrogen/Thermo Fisher Scientific) dissolved in normal Ca2+-buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher] ...
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bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher], 2 ng/ml GDNF [Invitrogen/Thermo Fisher] and 10 ng/ml NT4 [PeproTech ...
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bioRxiv - Neuroscience 2024Quote: ... 1x N2 (Life Technologies, Carlsbad, CA) and 1x B27 (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... and commercially available TaqMan primers (Invitrogen) for synaptophysin (Mm00436850_m1) ...
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bioRxiv - Neuroscience 2024Quote: ... Neurotrace 435/455 (1:250, ThermoFisher N21479) was added to the secondary antibody solution ...
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bioRxiv - Neuroscience 2024Quote: RNA was isolated from the remainder of the synaptosomal preparations using Tri-Reagent (Thermo Fisher, Waltham, MA) using the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... mounted in Permount (Fisher Scientific, Pittsburg, PA) and cover slipped ...
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bioRxiv - Neuroscience 2024Quote: ... they were loaded with 2 µM Fura-2-AM (Invitrogen/Thermo Fisher Scientific) dissolved in normal Ca2+-buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... 0.25x penicillin/streptomycin (Gibco, Grand Island, NY), 1x N2 (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Non-Essential-Amino-Acids (all from Invitrogen/Thermo Fisher). G418 (500 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... and Goat anti-Mouse-Alexa 633 (1:1000, Invitrogen). Neurotrace 435/455 (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti-Rat-Alexa 594 (1:1000, Invitrogen), and Goat anti-Mouse-Alexa 633 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher] ...
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bioRxiv - Neuroscience 2024Quote: ... 50% Neurobasal (Gibco, Grand Island, NY), 2.5 μg/ml human insulin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: rat anti-mCherry (1:250, ThermoFisher M11217), Mouse anti-Parvalbumin (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... and plated again in a dilution 1:6 in neural differentiation medium to induce neuroepithelial differentiation (NDM; 50% DMEM-F12 (Gibco, Grand Island, NY), 50% Neurobasal (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... Each purified library was quantified using a Qubit fluorometer (Life Technologies) and equal amounts of each library were pooled and submitted for sequencing on the Illumina NextSeq 500 by the DNA Sequencing and Genotyping Core at CCHMC ...
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bioRxiv - Neuroscience 2024Quote: ... 1x non-essential amino acid (NEAA; Gibco, Grand Island, NY), 0.25x penicillin/streptomycin (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was reverse transcribed with the Superscript III First Strand Synthesis kit (Thermo Fisher, Waltham, MA) to generate cDNA as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... DRGs or TGs were isolated and digested with 1 mg/ml collagenase and 2.5 mg/ml dispase dissolved in ‘basal medium’ (Neurobasal A medium supplemented with 10% FCS) (all from Gibco/Thermo Fisher) at 37°C for ca ...
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bioRxiv - Neuroscience 2024Quote: ... Relative gene expression was determined using TaqMan Gene Expression Master Mix (Invitrogen) and commercially available TaqMan primers (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... 1,1’-Dioctadecyl-3,3,3’,3’- Tetramethylindocarbocyanine Perchlorate (DiI, D282, Invitrogen, Carlsbad) was delivered via intracardiac injection prior to, ...
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bioRxiv - Neuroscience 2024Quote: ... Non-Essential-Amino-Acids (all from Invitrogen/Thermo Fisher). G418 (500 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... DRGs or TGs were isolated and digested with 1 mg/ml collagenase and 2.5 mg/ml dispase dissolved in ‘basal medium’ (Neurobasal A medium supplemented with 10% FCS) (all from Gibco/Thermo Fisher) at 37°C for ca ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with primary antibody directed against Aβ (Invitrogen, beta Amyloid (1-40) Polyclonal Antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher], 2 ng/ml GDNF [Invitrogen/Thermo Fisher] and 10 ng/ml NT4 [PeproTech ...
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bioRxiv - Neuroscience 2024Quote: ... or DiD (Cat.Nos.V22885, V22886, and V22887; Thermo Fisher Scientific) by repeatedly moving a drop of dye along the probe shank using a pipette until a dye residue was visible along its entire length (∼20 passes) ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative PCR (qPCR) was performed on a StepOne Plus Machine (Applied Biosystems) and analyzed using the delta-delta Ct method.
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 1% NEAA (Gibco, Grand Island, NY), 1% N2 (Gibco ...
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bioRxiv - Plant Biology 2024Quote: ... HRP signal was detected using chemiluminiscence (Thermo Scientific Pierce ECL Western Blotting Substrate Cat# PI32109).
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bioRxiv - Plant Biology 2024Quote: ... The mass spectra were acquired using a Q-exactive Orbitrap high-resolution mass spectrometer: Fourier-transform mass spectrometer (FT-MS) (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and resuspended in DOX-induction medium (DMEM-F12 (Gibco, Grand Island, NY) supplemented with 1% NEAA (Gibco ...
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bioRxiv - Plant Biology 2024Quote: ... The acidified tryptic peptides were desalted using C18 Tips (Thermo Fisher Scientific, 10 μL bed, 87782) and peptide samples were eluted with 0.1% formic acid and analysed on an Orbitrap Elite instrument (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... [38] attached to an EASY-nLC 1000 liquid chromatography system (Thermo Fisher Scientific). Samples were separated on an analytical column based on a fused silica capillary with an integrated PicoFrit emitter (New Objective ...
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bioRxiv - Plant Biology 2024Quote: Mouse liver microsomes (purchased from Thermo: GIBCO Mouse (CD-1) Microsomes ...