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Citations for Thermo Fisher :
6351 - 6400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Eluted RNA was subsequently extracted from the beads by the Trizol reagent (Invitrogen). The isolated RNA was quantified ...
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bioRxiv - Cancer Biology 2024Quote: ... 1x glutamine (Gibco 25030024) and 1x PenStrep (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1x PenStrep (Gibco, 15070-063). SUM159 cell line was cultured in Ham’s-F12 (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... fluorescently labeled secondary antibodies (Molecular Probes, Thermo) were added after washing away unbound primary antibodies and incubated at room temperature for at least 2 hr or 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with Fetal Bovine Serum 10% (Gibco 10270106), 1x glutamine (Gibco 25030024 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1x PenStrep (Gibco, 15070-063).
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bioRxiv - Cancer Biology 2024Quote: ... The resulting amplified cDNA library was quantified using the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher) via qPCR ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 0.1% BSA (Ambion, # AM2616). After 5 minutes centrifugation (179 × g for 5 min) ...
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bioRxiv - Cancer Biology 2024Quote: ... collected microstructures were incubated with pre-warmed (37 °C) TrypLE (Gibco, #12604013) for 15 minutes in a shaker at 100 rpm and 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Microstructures were then collected into a 50-ml conical tube by centrifugation (179 × g for 5 min) and washed with HBSS without calcium and magnesium (Gibco, #14175095) containing 0.1% BSA (Ambion ...
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bioRxiv - Neuroscience 2024Quote: PolyA RNA was purified with Dynabeads mRNA Purification Kit (Ambion, Naugatuck, USA)74 ...
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bioRxiv - Cancer Biology 2024Quote: ... The purified DNA was quantified using Qubit (Invitrogen) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 1% N2 supplement (ThermoFisher, 17502001), 0.1% B27 supplement (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with H14 media additives plus Antibiotic-Antimycotic (Gibco, #15240096) and added to ultra-Low Attachment Surface six-well plate (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA quantification was performed using Qubit (Invitrogen), and fragment sizes of individual libraries were analyzed using the High Sensitivity DNA Kit (Bioanalyzer®) ...
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bioRxiv - Cancer Biology 2024Quote: ... BE(2)-C cells and SK-N-AS cells were grown in 75 cm2 cell culture flasks in Dulbecco’s Modified Eagle’s Medium (DMEM) + 10% fetal calf serum (FCS) (Life Technologies), and stable ATP13A3 KD and overexpression SH-SY5Y cells were cultured in DMEM with high glucose supplemented with sodium bicarbonate ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µm-thick tissue sections were cut at -20°C using a Cryostar NX70 (Thermo Fisher), placed onto the Visium slides ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1x PenStrep (Gibco, 15070-063). MDA-MB-231 cell line was cultured in DMEM (Gibco 11960044 ...
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bioRxiv - Cancer Biology 2024Quote: ... hydrocortisone 1 μg/ml (Voden, 74144) and Fetal Bovine Serum 5% (Gibco 10270106) and 1x PenStrep (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in 1 ml TRIzol (Invitrogen, 15596018). Total RNA was extracted by phenol/chloroform and purified through the RNA Clean and Concentrator-25 kit (Zymo Research ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 μg/mL Glycogen (Thermo Scientific, R0561), and distilled water ...
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bioRxiv - Cancer Biology 2024Quote: ... Nascent DNA was labelled through EU incorporation (Click-iT® RNA Imaging Kits, Invitrogen, cat. C10329) for 1h hour starting from either ...
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bioRxiv - Cancer Biology 2024Quote: ... Next the complexes were captured on ProteinA/G agarose beads (ThermoFisher Scientific R0561), washed ...
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bioRxiv - Cancer Biology 2024Quote: ... and Alexa Fluor 488 anti-mouse (Invitrogen, 11001) 1:500 in blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were phenol/chloroform extracted and quantified using a Qubit™ 4 Fluorometer (Invitrogen, Q33238) using the Qubit™ 1X dsDNA High Sensitivity kit (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Qubit™ 1X dsDNA High Sensitivity kit (Invitrogen, Q33265). 3 ng of DNA were used to perform library preparation with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7645) ...
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bioRxiv - Bioengineering 2024Quote: ... MS2 fragmentation spectra were searched and analyzed using Sequest and XlinkX nodes bundled into Proteome Discoverer (version 2.5, Thermo Scientific) against in silico tryptic digested protein sequences including AAV9 capsid protein VP1 and Interleukin-3 retrieved from Uni-Prot (Q6JC40 and Q6NZ78 ...
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bioRxiv - Bioengineering 2024Quote: ... and human and mouse DKK3 (hDKK3: AA 1-350, mDKK3: AA 1-349) triple tagged with Fc-Myc-6xHis were transfected into Expi293FTM (Thermo Fisher Scientific) cells at a density of 3 × 106 viable cells/mL using ExpiFectamineTM (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane permeability of the peptide was determined by using the LIVE/DEAD BacLight Bacterial Viability Kit (Invitrogen). S ...
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bioRxiv - Bioengineering 2024Quote: The cross-linking procedure was modified from the manufacturer’s instructions (Thermo Fisher). In brief ...
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bioRxiv - Bioengineering 2024Quote: ... LC-MS/MS analysis was performed with an EASY-nLC 1200 (ThermoFisher Scientific, San Jose, CA) coupled to a Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Bis(sulfosuccinimidyl)suberate (BS3) (Thermo Fisher) was added to a final concentration of 3 mM and incubated at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... cells at a density of 3 × 106 viable cells/mL using ExpiFectamineTM (Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Bioengineering 2024Quote: ... coupled to a Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (ThermoFisher Scientific, San Jose, CA). Peptides were separated on an Aurora UHPLC Column (25 cm × 75 μm ...
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bioRxiv - Biophysics 2024Quote: ... Isolated RNA was reversely transcribed using High-Capacity cDNA Reverse Transcription Kit (ThermoFisher #4368813). cDNA was diluted 1:10 in H2O and was used for quantitative PCR using Power SYBR Green PCR Master Mix (ThermoFisher #4368708 ...
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bioRxiv - Biophysics 2024Quote: ... individual 20 μl reactions were prepared containing 1μM of ctNAT10 and a 1:1000 dilution of a 5000× concentrated SYPRO orange dye (Thermo Fisher Scientific) in DSF buffer (37.5 mM Hepes-NaOH pH = 7.5 ...
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bioRxiv - Biophysics 2024Quote: Total RNA (5 µg) from each yeast transformant was treated with TURBO DNase (Invitrogen) by adding 1 µl of the enzyme ...
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bioRxiv - Biophysics 2024Quote: ... cDNA was diluted 1:10 in H2O and was used for quantitative PCR using Power SYBR Green PCR Master Mix (ThermoFisher #4368708) following manufacturer protocol and run on Applied Biosystem QuantStudio Real-Time PCR System using targeted primer and reference primer sets (See Key Resource Table S4).
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... and were converted into cDNA using SUPERSCRIPT IV VILO MASTERMIX (Thermofisher Scientific, Cat#11756050). Quantitative real-time PCR was performed using Applied Biosystems PowerUp SYBR Green Master Mix (Fisher Scientific ...
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bioRxiv - Zoology 2024Quote: ... injected embryos were frequently cultured through stage 8 in HL saline containing roughly 1% commercial penicillin-streptomycin solution (Gibco).
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bioRxiv - Zoology 2024Quote: ... peptides were separated on an EASY nanoLC 1000 UPLC system (Thermo Fisher Scientific, Waltham, MA) using RPC18 columns 50 cm (fused Silica tube with ID 50μm ± 3μm ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... We used the Ion Chef System for automated library preparations with the Ion 540 kit (Thermofisher Scientific, Cat#A30011). Pooled barcoded libraries were subsequently loaded into the Ion GeneStudio S5 sequencer for high throughput sequencing to obtain 60-80 million reads per run.
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bioRxiv - Zoology 2024Quote: ... using PowerUPTM SYBR® Green Master Mix (Applied Biosystems, Carlsbad, CA, United States). The correctness of amplicons was confirmed by Sanger sequencing (Center for Applied ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... Stained cells were mounted using Prolong™ Glass Antifade Mountant (Thermofisher Scientific, Cat#P36982) and stored at 4°C ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... Ion AmpliSeq transcriptome human gene expression panel (Thermofisher Scientific, Cat#A31446) was added to the Reagents cartridge ...
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bioRxiv - Biophysics 2024Quote: ... 1mL Ni2+ agarose bead (Cat # R90101, ThermoFisher Scientific, Waltham, MA) slurry per liter of cell culture were washed in wash buffer 1 (50 mM Tris pH 8 ...
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bioRxiv - Biophysics 2024Quote: ... were pre-mixed in 1 mL of Opti-MEM media (Life Technologies Cat #:51985-034) and incubated for 20 min ...
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bioRxiv - Biophysics 2024Quote: ... with 2.5 μl Novex™ Hi-Density TBE Sample Buffer (5X) (Invitrogen). The gel was run in 0.5x TBE buffer at 120 V for 42 min and stained with 1x SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: ... supplemented with 15% fetal bovine serum (Gibco™, A3840002), 2 mM L-glutamine (Gibco™ ...
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bioRxiv - Systems Biology 2024Quote: ... 0.1 mM beta-mercaptoethanol (Gibco™ 31350010) and 500 U/mL recombinant leukemia inhibitory factor (LIF ...