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Citations for Thermo Fisher :
6701 - 6750 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... converted into cDNA using the SuperScript III First-Strand Synthesis SuperMix kit (Invitrogen), and treated with RNase A/T1 Mix (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and treated with RNase A/T1 Mix (Thermo Scientific). The final cDNA sample was then split into 16 separate reactions for final PCR amplification using 16 unique Illumina indexing primers and Q5 Hot Start High-Fidelity 2X Master Mix (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies (goat anti-guinea pig Alexa Fluor 647 (1:1000; Invitrogen), goat anti-rabbit Alexa Fluor 568 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibodies (donkey anti-rabbit Alexa Fluor Plus 488, Invitrogen, A32790, 1:1000; goat anti-mouse Alexa Fluor Plus 555, Invitrogen, A32727, 1:1000) were diluted with blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... After washing 3 times with PBST, the slides were incubated with DAPI (Beyotime, C1005) and then mounted with ProLong Glass Antifade Mountant (Invitrogen, P36980). After the mounting medium was allowed to dry for 10 h ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-rabbit Alexa Fluor 568 (1:1000; Invitrogen), goat anti-mouse Alexa Fluor 488 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were blocked in blocking buffer consisting of 5% normal goat serum (Invitrogen) in PBS for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted onto glass slides and coverslipped using ProLong diamond anti-fade reagent (Molecular probes/Life technologies). The sections were imaged using a confocal laser scanning microscope (Zeiss LSM900-Airyscan confocal ...
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bioRxiv - Neuroscience 2024Quote: Mouse hippocampal tissue was disrupted and homogenized in 1xRIPA buffer (#89901, Thermo Fisher Scientific) containing phosphatase and protease inhibitors (#P8340 and #P0044 ...
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bioRxiv - Neuroscience 2024Quote: Each sample underwent reverse transcription of 1 µg of total RNA using random primers and the High Capacity cDNA reverse transcription kit (Thermo Fisher Scientific. USA). Reverse transcription was performed on a VWR Ristretto thermal cycler (VWR International GmbH ...
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bioRxiv - Neuroscience 2024Quote: ... A ViiA™ 7 Real-Time PCR System (Applied Biosystems. USA) was used to perform RT-qPCR ...
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bioRxiv - Neuroscience 2024Quote: ... 1:1000 anti-KV3.1 (KCNC1) polyclonal rabbit antibody (#PA5-101763, Invitrogen), 1:1000 anti-Synaptotagmin-2 polyclonal rabbit antibody (#60889 ...
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bioRxiv - Neuroscience 2024Quote: ... Hoechst 33342 (Molecular Probes/Life Technologies) was used as a nuclear counterstain ...
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bioRxiv - Neuroscience 2024Quote: ... was used to obtain information about the quantity (Qubit RNA BR Assay Kit; Invitrogen. USA) and quality (Qubit RNA IQ Assay Kit ...
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bioRxiv - Neuroscience 2024Quote: ... The cDNA concentrations were measured using a Qubit ssDNA assay kit (Invitrogen. USA) on a Qubit 4 Fluorometer.
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bioRxiv - Neuroscience 2024Quote: ... The expression of 45 genes associated with either the eCB system or neuronal activity and 3 housekeeping genes were analyzed using Custom 384-well microfluidic TaqMan® Gene Expression Array Cards (Applied Biosystems. USA). The array cards consisted of eight fill reservoirs linked to a series of 48 reaction wells ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted onto glass slides and coverslipped using ProLong diamond anti-fade reagent (Molecular probes/Life technologies). The sections were imaged using a confocal laser scanning microscope (Zeiss LSM900-Airyscan confocal ...
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bioRxiv - Neuroscience 2024Quote: FAM-labeled TaqMan assays (ThermoFisher) used were ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was then quantified in a nanodrop and reverse-transcribed using the SuperScript III First-Strand Synthesis System (#18080051, ThermoFisher), followed by RNAse H digestion ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-mouse Alexa Fluor 488 (1:1000, Invitrogen)) were incubated for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and quality (Qubit RNA IQ Assay Kit; Invitrogen. USA) of the RNA in the samples ...
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bioRxiv - Neuroscience 2024Quote: ... Hoechst 33342 (Molecular Probes/Life Technologies) was used as a nuclear counterstain ...
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bioRxiv - Neuroscience 2024Quote: ... and its concentration was quantified by Nanodrop 2000 C Spectrophotometer (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... 1:1000 anti-KCNS3 polyclonal rabbit antibody (#PA5-96856, Invitrogen), 1:1000 anti-Nav1.1 Na+ Channel monoclonal mouse antibody (#75-023 ...
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bioRxiv - Neuroscience 2024Quote: ... The Qubit 4 Fluorometer (Invitrogen. USA) was used to obtain information about the quantity (Qubit RNA BR Assay Kit ...
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bioRxiv - Neuroscience 2024Quote: ... following the manufacturer’s instructions (#12183018A, ThermoFisher). RNA was then quantified in a nanodrop and reverse-transcribed using the SuperScript III First-Strand Synthesis System (#18080051 ...
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bioRxiv - Neuroscience 2024Quote: ... The cycle threshold (Ct) values and other data related to PCR reactions were collected using the QuantStudio software (Applied Biosystems. USA). The gene expression levels for the candidate genes were normalized to the geometric mean of expression levels of the three endogenous control genes (18S ...
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bioRxiv - Neuroscience 2024Quote: ... respectively (1:500; Invitrogen A31573, Invitrogen A11055, Jackson IR 703-545-155). After additional incubation in DAPI (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were measured at 562nm using the Pierce Bicinchoninic Acid (BCA) kit (Thermo Scientific; 10678484). Proteins were prepared for SDS-PAGE separation by denaturation with 2x Laemmli sample buffer (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... The backbone vector used in this study is pcDNA3.1 (Invitrogen) with a CMV promoter.
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bioRxiv - Neuroscience 2024Quote: ... 100 mM dextrose in ddH2O) mixed with 52 mM bis(sulfosuccinimidyl)suberate (BS3; ThermoFisher Scientific, catalog 21580), a membrane-impermeable bifunctional crosslinking agent ...
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bioRxiv - Neuroscience 2024Quote: ... HEK 293 cells were suspended using 0.25% Trypsin-EDTA (Gibco, USA) then plated onto poly-L-lysine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Compensation control was performed using Ultracomp beads (Thermo Fisher) using optimal antibody concentration determined by titration ...
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bioRxiv - Neuroscience 2024Quote: ... 488-anti-mouse (Invitrogen, A28175, 1:500), 555-anti-rabbit (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates underwent immunoprecipitation using Dynabeads Protein A (Thermo Fisher Scientific) with either anti-p-UPF1 S1116 (MilliporeSigma ...
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bioRxiv - Neuroscience 2024Quote: ... and RNA fragments were purified using TRIzol Reagent (Thermo Fisher Scientific). Electrophoresis in 6M urea–15% polyacrylamide parallel to a DynaMarker Prestain Marker for Small RNA (BioDynamics Laboratory ...
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bioRxiv - Neuroscience 2024Quote: ... Cellular RNAs captured by bead-bound antibody complexes were digested to <100 nt by incubating for 30 min at 4°C with RNase I (1 U/μl; Thermo Fisher Scientific Ambion; AM2294). After extensive washing ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5% w/w Triton X-100) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific). The lysates underwent immunoprecipitation using Dynabeads Protein A (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Cellular RNAs captured by bead-bound antibody complexes were digested to <100 nt by incubating for 30 min at 4°C with RNase I (1 U/μl; Thermo Fisher Scientific Ambion; AM2294). After extensive washing ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected using Lipofectamine RNAiMAX (Invitrogen) according to manufacturer guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... pre-designed siRNAs (Ambion Silencer Select or Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Cells were harversted in 1mL TRIzol Reagent (Thermo Fisher Scientific) per 1-3 million cells ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription of the adapter-ligated RNAs using SuperScript III reverse transcriptase (Thermo Fisher Scientific) was followed by PCR amplification using Phusion high-fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... Microscopic samples were mounted on glass slides using ProLong Antifade reagent (Thermo Fisher Scientific). Images were captured using an Olympus FV10i microscope with a 60x/1.35 oil immersion objective and analyzed using ImageJ 1.52N software (National Institutes of Health) ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were then mounted in Fluoromount (ThermoFisher Scientific, Waltham, MA) and scanned using a Hamamatsu NanoZoomer 2.0 digital slide scanner (Hamamatsu Photonics K.K. ...
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bioRxiv - Neuroscience 2024Quote: ... DNA yield was then measured using Qubit 3 (High Sensitivity DNA Assay, ThermoFisher). Libraries were prepared using 2 ng of ChIP DNA and 10 ng of Input DNA (KAPA Hyper Prep Kit ...
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bioRxiv - Neuroscience 2024Quote: Click-iT™ EdU Cell Proliferation Kit (Invitrogen, C10337) was used to measure cell proliferation as descried in the protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted onto slides (Superfrost Plus) and cover–slipped using ProLong Gold Antifade Reagent (Thermo Fisher Scientific, catalog no. P36982). All steps were performed at room temperature.
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bioRxiv - Neuroscience 2024Quote: ... A BCA protein assay kit (ThermoFisher, catalog number: 23225) was used to determine protein concentration ...
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bioRxiv - Neuroscience 2024Quote: HEK 293 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, USA) supplemented with 10% Fetal Bovine Serum (FBS ...