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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2024Quote: ... Excreta were then collected over intervals of 1h-1h30 in 500 µl of DMEM containing 1% antibiotic-antimycotic (Gibco, France). Before adding media ...
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bioRxiv - Zoology 2024Quote: ... 2% FBS and 1% of antibiotic-antimytotic (Gibco, France). Cells were incubated at 37°C with 5% CO2 for 7 days ...
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bioRxiv - Zoology 2024Quote: ... Excreta were collected in 500 µl of DMEM containing 1% antibiotic-antimycotic (Gibco, France) on the 4th ...
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bioRxiv - Biophysics 2024Quote: ... following ABI assay ID data base (Thermo Fisher). The qPCR sets of primers for ITGA1 (Hs00235006_m1) ...
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bioRxiv - Zoology 2024Quote: Diatoms and other microalgae species were cultured in 50 ml vented flasks (BioLite 25cm2, ThermoFisher Scientific 130189) with 40 ml culture medium (autoclaved FSW containing F/2 nutrient medium at 1 ml per 2.5 L (ZM Systems) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with penicillin (100 I.U/mL, Cellpure®), streptomycin (0.1 mg/mL, Cellpure®) and fetal bovine serum (Brazilian origin, Gibco). Cells were seeded on μ-Dishes (35mm μ-Dish ...
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bioRxiv - Neuroscience 2024Quote: ... and α-tubulin (ThermoFisher, 62204 ...
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bioRxiv - Neuroscience 2024Quote: ... denatured in LDS sample buffer (ThermoFisher, NP0007) with a reducing agent (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: Protein extracts were prepared in radioimmunoprecipitation assay lysis buffer (ThermoFisher, 89900) with protease and phosphatase inhibitors (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... and Dycle Ruby (Invitrogen, Cat. No. V10309) were used to sort the cells by FACS ...
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bioRxiv - Neuroscience 2024Quote: ... and used as template DNA to generate each sgRNA by in vitro transcription using MEGAshortscript T7 kit (Invitrogen). The size and quality of the gRNAs were visualized in %3 agarose gel electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... with protease and phosphatase inhibitors (ThermoFisher, 87786). Lysates were sonicated at 4 °C (Bioruptor Pico) ...
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bioRxiv - Neuroscience 2024Quote: ... (pCS2-Cas9) was linearized by NotI digestion and then purified by reaction purification kit (Invitrogen). Using mMESSAGE mMACHINE SP6 kit (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Viability indicator dyes Sytox Blue (Invitrogen, Cat No. S34857) and Dycle Ruby (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... SYBR Green (Applied Biosystems) based qPCR was performed on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System ...
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bioRxiv - Neuroscience 2024Quote: ... and reverse transcribed to generate cDNA (ThermoFisher, 11756500). PCR was performed using 10-30 ng cDNA template ...
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bioRxiv - Neuroscience 2024Quote: ... TAU-5 (ThermoFisher, AHB0042, 1:2000), and α-tubulin (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... 50% Ham’s F12 (ThermoFisher, 11765047), supplemented with 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2024Quote: ... with a reducing agent (ThermoFisher, NP0009), and heated at 70 °C for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... The recording ACSF consisted of (in mM): 126 NaCl (Fisher Scientific), 25 NaHCO3 ...
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bioRxiv - Neuroscience 2024Quote: ... a two-photon microscope turned to 920-nm and 1045-nm wavelength lasers were used to acquire images after intravenous injection of Dextran-Texas Red (Thermo Fisher Scientific, D1830) and DIR antibodies.
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bioRxiv - Neuroscience 2024Quote: ... and GFP (Invitrogen, A11122).
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bioRxiv - Neuroscience 2024Quote: ... and 0.4 U/μl RNase inhibitor (Invitrogen, Carlsbad, USA). Tissues were transferred into a 2 ml Dounce Tissue Grinder (Kimble Chase ...
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bioRxiv - Neuroscience 2024Quote: ... The NPCs were maintained in Dulbecco’s modified eagle’s medium: F12 (DMEM: F12, Thermo Fisher) containing 1×B27 (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... Then tissue sections were washed with PBS for three times and incubated with Alexa fluorescence-conjugated secondary antibodies (Invitrogen) for 60 min at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were washed and then developed by adding Slow Step ELISA TMB substrate (Thermofisher, Cat: 34024) to each well ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or a combination of lead and aptamers (10nM, 25nM and 50nM) in Opti-MEM (reduced serum medium) using Lipofectamine RNAi MAX reagent from Invitrogen for 6 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were incubated with 100ul of tenfold diluted Alamar Blue solution (Invitrogen, DAL1100). After a 3-hour incubation period ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... LC-MS/MS experiments were performed on an Orbitrap Exploris 480 equipped with an UltiMate 3000_UPLC system (Thermo Scientific, Waltham, MA, USA) as described above.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Potassium phosphate 0.5 M buffer solution pH 7.4 was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... was performed on Machery & Nagel Alugram® xtra SIL G/UV 254 visualization under UV light (254 nm) and/or (366 nm) and/or by dipping in anisaldehyde stain and subsequent heating.Commercial reagents and solvents (Acros Organics, Fluorochem ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and cDNA was synthesized with 1ug of RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). SYBR green chemistry was used for quantitative determination of the various mRNAs for Runx-2 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... All DIA-MS experiments were performed on an Orbitrap Exploris 480 equipped with an UltiMate 3000_UPLC system (Thermo Scientific, Waltham, MA, USA). The project-specific DIA spectral library was first generated by DIA-MS2pep as previously described48 ...
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bioRxiv - Physiology 2024Quote: ... and incubated for 1h at RT with a chicken anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies, Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then incubated with anti-rat FoxP3 (Invitrogen) for 30 minutes ...
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bioRxiv - Physiology 2024Quote: Cell volume was measured as fluorescence in cells loaded with 10 mM Calcein AM (ThermoFisher Scientific) at RT for 30 min ...
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bioRxiv - Physiology 2024Quote: Cardiac RNA was isolated from 3- and 18-week-old hearts that were stored at −80°C in 500 μL of RNAlater™ Stabilization Solution (Invitrogen, Cat. No. AM7020). Total RNA was isolated by homogenization with T10 basic ULTRA-TURRAX® (IKA) ...
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bioRxiv - Physiology 2024Quote: ... cells were incubated with chicken anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies, Thermo Fisher, diluted 1:500 in FBS) for 1h at RT ...
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bioRxiv - Physiology 2024Quote: ... the excess antibody was removed using 10-min washes with TBS-T and a goat anti-rabbit antibody conjugated with Alexa Fluor 488 (Life Technologies, Thermo Fisher scientific ...
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bioRxiv - Physiology 2024Quote: ... the excess antibody was removed using 10-min washes with TBS-T and a goat anti-rabbit antibody conjugated with Alexa Fluor 488 (Life Technologies, Thermo Fisher scientific, diluted 1:5000 in TBS-T) was added for 1 h at RT ...
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bioRxiv - Physiology 2024Quote: ... neonatal hearts from 1-2 days pups were dissected in cold Hanks’ Balanced Salt Solution (HBSS) and the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher, 88281) was used to obtain neonatal cardiomyocytes ...
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bioRxiv - Physiology 2024Quote: ... Concentration of eluted DNA and RNA were verified with Qubit dsDNA High Sensitivity or RNA High Sensitivity assays (Thermo Fisher Scientific), respectively ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Splenocytes were incubated at 4°C and washed three times with FACS buffer and fixed with FoxP3/Transcription Factor Staining Buffer Set (Invitrogen) fixative and stored at 4°C until intracellular staining ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Nanodrop UV quantitative system (Thermo Fisher Scientific, NC2000), Sequencer (Illumina ...
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bioRxiv - Physiology 2024Quote: ... Results were visualized in an iBright 1500 reader (Invitrogen, Thermo Fisher). cMyBP-C levels normalized to GAPDH were quantified using Quantity One software ...
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bioRxiv - Physiology 2024Quote: ... species-matched Alexa Fluor 488 labelled secondary antibody was used (Thermo Fisher W11261 ...
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bioRxiv - Physiology 2024Quote: ... cDNA samples were diluted 20 times and used for qPCR using Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific, Cat. No. 4367660) in a Bio-Rad CFX384 thermal cycler using specific primers sets for Mybpc3 (Forward ...
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bioRxiv - Physiology 2024Quote: ... and incubated for 1h at RT with a chicken anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies, Thermo Fisher, diluted 1:5000 in TBS-T). After incubation with the secondary antibody ...
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bioRxiv - Physiology 2024Quote: ... Results were visualized in an iBright 1500 reader (Invitrogen, Thermo Fisher). cMyBP-C levels normalized to GAPDH were quantified using Quantity One software ...
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bioRxiv - Physiology 2024Quote: ... using a UPLC-Ultimate 3000 chromatography system connected to a Q-Exactive HF mass spectrometer (Thermo Fisher). Mass spectra were acquired in a data-dependent manner ...