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Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 25 ul alamarBlue Cell Viability Reagent (DAL1025, ThermoFisher, Waltham, MA) was added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... RNA samples were promptly processed for complementary DNA preparation using the SuperScript IV First-Strand Synthesis System (Invitrogen, 18091200).
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bioRxiv - Cell Biology 2024Quote: ... and those from PERK-/- mouse samples were collected on a Titan Krios (Thermo Fisher Scientific). The detailed information and parameters of data collection are listed in Table S1.
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bioRxiv - Cancer Biology 2024Quote: ... claudin 4 (ZMD.306, Invitrogen, 1:250), and PRKCDBP (Cavin3 ...
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bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
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bioRxiv - Cell Biology 2024Quote: ... along with the TaqMan Gene Expression Master Mix (Applied Biosystems, 4369510), were employed for qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... the stained samples were carefully mounted with glass coverslips using Fluoromount-G (Invitrogen, 00-4958-02). To validate specificity ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GM130 (MA5-35107) (Fisher Scientific, Pittsburgh, PA, USA), anti-EEA1 (24115) ...
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bioRxiv - Cell Biology 2024Quote: ... EM grids were imaged using a Philips CM10 electron microscope (Thermo Fisher Scientific), operated at 100 kV and at nominal magnification of 52,000x ...
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bioRxiv - Cell Biology 2024Quote: Lamellae were prepared by cryo-FIB milling using an Aquilos microscope (Thermo Scientific) as described previously 53 ...
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bioRxiv - Cancer Biology 2024Quote: ... 27) were plated on 96-well plates (103 cells/well) and grown in DMEM (Dulbecco’s Modified Eagle Medium, Gibco), supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... or S6 (E.573.4, rabbit, Invitrogen, MA5-15164, 1:1000), and followed by one-hour incubation with diluted secondary antibody (1706515 Goat Anti-Rabbit IgG-HRP Conjugate ...
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bioRxiv - Cell Biology 2024Quote: ... The absorption spectra of ER-Tracker and Mito-Tracker were measured using a UV-VIS spectrometer (GENESYS 50, Thermofisher).
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bioRxiv - Cancer Biology 2024Quote: ... Organoids were monitored and analysed twice a week using EVOS M7000 Imaging System (Thermo Fisher Scientific, Waltham, MA), whereby the numbers and sizes of organoids greater than 100 µm in diameter were recorded ...
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bioRxiv - Cell Biology 2024Quote: MCF10A cells were cultured in growth media composed of DMEM/F12 (Invitrogen #11330-032), 5% horse serum (Sigma #H1138) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed with DPBS (Dulbecco’s phosphate buffered saline, ThermoFisher Scientific, Massachusetts, USA) and fixed with 4% Formaldehyde for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and 7 μm sections were placed onto Superfrost Plus slides (Fisher Scientific). Tissue sections were processed for routine histology and stained with hematoxylin and eosin reagents (Sigma-Aldrich St ...
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bioRxiv - Cell Biology 2024Quote: ... washed again with PBS and then counter-stained with nuclear Hoechst (Invitrogen Waltham, MA). Slides were mounted with a 60% glycerol solution and imaged ...
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bioRxiv - Cell Biology 2024Quote: ... Stem Cell Medium was made of DMEM (Gibco; # 11995065) supplemented with 20% FBS (Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were blocked in 5% normal serum (Thermo Fisher Scientific, 10000 C) in PBS-T (0.4% Triton X-100 in PBS ...
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Pathway metabolite ratios reveal distinctive glutamine metabolism in a subset of proliferating cellsbioRxiv - Cancer Biology 2024Quote: ... Culturing media were supplemented with 10% fetal bovine serum (Cytiva Hyclone) and 1% penicillin/streptomycin (Gibco) unless stated otherwise (Appendix table 1) ...
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bioRxiv - Cancer Biology 2024Quote: ... with Geneticin G418 Sulphate (0.6 mg/ml, 11811-031, Gibco) for CAIX-transfected lines ...
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bioRxiv - Cancer Biology 2024Quote: ... and non-essential amino acids (0.1 mM Gibco) and sodium pyruvate (1 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µl 10% SDS plus 2 µl proteinase K (Thermo Fisher Scientific, Cat# EO0492) were added and incubated for 60 min 37°C ...
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Pathway metabolite ratios reveal distinctive glutamine metabolism in a subset of proliferating cellsbioRxiv - Cancer Biology 2024Quote: Cells were seeded in 96-well plates (Nunc) in 100 mL basal medium ...
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bioRxiv - Cancer Biology 2024Quote: ... MEM Sodium Pyruvate Solution (Invitrogen, 11360-070), MEM Non-Essential Amino Acids Solution (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... The following additive were added freshly: B27-A (1:50, Invitrogen, 12587010), human FGF (20 ng/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... MEM Non-Essential Amino Acids Solution (Invitrogen, 11140-050), GlutaMAX-I (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... and Neurobasal-A (Invitrogen, 10888022) media ...
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bioRxiv - Cell Biology 2024Quote: ... The sections were washed with PBS-T (0.4% Triton X-100 in PBS) and mounted with ProLong® Gold Antifade Mountant with DAPI (Thermo Fisher Scientific, P36934). All mounted slides were visualized using a Zeiss LSM 800 Axio Inverted confocal microscope (Plan-Apochromat 5x/0.16 or 10x/0.45) ...
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bioRxiv - Cell Biology 2024Quote: Intracellular calcium concentration ([Ca2+]i) was measured using the Fura-2-AM calcium probe (ThermoFisher Scientific). Healthy and DMD hiPSC-derived muscle cells at D3 and D7 were loaded with Fura-2 AM (3 μM ...
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bioRxiv - Cell Biology 2024Quote: ... The specimens were minced into small pieces in phosphate-buffered saline solution (PBS) and directly cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, 12800-017, Massachusetts, USA) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 555 conjugated goat anti-chicken and Alexa Fluor 647 Donkey anti-mouse (1:500, ThermoFisher Scientific). Cells were mounted in Mowiol medium (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... The mitochondrial DNA (mtDNA) content was determined using qPCR (Applied Biosystems A25742 PowerUP SYBR green master mix). For mitochondrial DNA (mtDNA ...
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bioRxiv - Cell Biology 2024Quote: ... and then the medium was changed to StemPro-34 Serum-free Medium (SFM, Gibco) supplemented with 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... RPMI 1640 medium (with L-glutamine, Gibco) with B-27 supplement (minus vitamin A ...
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bioRxiv - Cell Biology 2024Quote: ... and then fixed with 4% paraformaldehyde and 0.2% saponin in PBS (Gibco). The fixed cells could be stored in 10% DMSO in FBS at -80°C for future use ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell pellets were resuspended in cold PBS containing 0.5% BSA and filtered through a cell strainer with 35μm sized mesh (Fisher Scientific 352235) to remove clumps ...
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bioRxiv - Cell Biology 2024Quote: ... The cells harvested on different days of cardiac differentiation were stained with cTnT antibody (Troponin T, mouse monoclonal antibody, Thermo Fisher Scientific, MS-295-P, 1:200) and then with donkey anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... 1% penicillin/streptomycin (Gibco; #15070063), 1% NEAA (Non-Essential Amino Acid ...
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bioRxiv - Cell Biology 2024Quote: ... with B-27 supplement (minus vitamin A, Gibco) was used for further cell culture of the differentiated myocytes.
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bioRxiv - Cell Biology 2024Quote: ... Lipofectamine 2000 (11668019) were obtained from Life Technologies (Carlsbad, CA, USA). Fluorescein sodium (46955) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged with a ratio of 1:4 to when they reached 70% to 80% confluency by incubation cells with 0.5 mM EDTA (Fisher Scientific MT-46034CI) at room temperature for 5 minutes.
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bioRxiv - Cell Biology 2024Quote: ... water (5:3:2) and 3 µL was injected into a Ultimate 3000 UHPLC system coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific). Separation was done on an Accucore C18 column (150 x 2.1 mm ...
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bioRxiv - Cell Biology 2024Quote: All the hPSCs are cultured on Matrigel (Fisher Scientific 08-774-552) in Stemflex Medium (Thermo Fisher A3349401 ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were treated with FastAP alkaline phosphatase and Exonuclease I (Thermo Scientific), and Sanger sequencing was performed on a 3130xl genetic analyzer (ABI) ...
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bioRxiv - Cell Biology 2024Quote: ... in Stemflex Medium (Thermo Fisher A3349401) and fed daily ...
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bioRxiv - Cell Biology 2024Quote: Cells were washed once with ice-cold PBS and subsequently lysed with RIPA Buffer (ThermoFisher, 89900), supplemented with protease inhibitor cocktails (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 20% FBS (Fetal Bovine Serum; Gibco; #10082147), 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Cell Biology 2024Quote: Mouse iPSCs and differentiated cells in tissue cultures were dissociated by incubation with Trypsin-EDTA (0.25%, Gibco) and then fixed with 4% paraformaldehyde and 0.2% saponin in PBS (Gibco) ...