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Citations for Thermo Fisher :
6501 - 6550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... supplemented with L-glutamine (2 mM, ThermoFisher #25030081), Penicillin-Streptomycin (100 Units/ml ...
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bioRxiv - Genetics 2024Quote: ... 2 x 105 cells were seeded in 6-well plates and grown overnight in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco #11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2024Quote: ... We evaluated the extracted DNA quality and quantity by agarose gel electrophoresis (1% w/v) stained with SYBR Safe DNA Gel Stain (Invitrogen) and by visual comparison with lambda DNA (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: ... coli (Invitrogen 12297016), followed by restriction digestion and insertion into pSM vector (78 ...
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bioRxiv - Genetics 2024Quote: ... while the starved condition was incubated in Hanks’ Balanced Salt Solution (HBSS) (Gibco #14175095) for 3 ...
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bioRxiv - Cell Biology 2024Quote: ... Penicillin-Streptomycin (100 Units/ml; 100 μg/ml respectively, ThermoFisher # 15140122), Activin A (100 ng/ml ...
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bioRxiv - Genetics 2024Quote: ... and RNAseOUT (Invitrogen 10777-019).
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bioRxiv - Genetics 2024Quote: ... Worms were starved at 20°C while being continuously rotated on a tube rotator (ThermoFisher) at 20 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... TrypLE Select Enzyme solution (ThermoFisher #12563011) was introduced to the bottom channel and chips were incubated for 10 minutes at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The organoids were washed twice in DPBS (Gibco, #14190-094), re-suspended in fresh Basement Membrane Matrigel and plated in domes as described before.
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA was extracted from Lbm and Lh individuals using the TRIzol reagent (Invitrogen), and RT-PCR experiments were performed using the RacGAP1-specific degenerate primers ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM sodium pyruvate (100 mM, #11360070, Gibco), 20 mM HEPES (1 M ...
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bioRxiv - Neuroscience 2024Quote: ... 100 U/ml Penicillin/streptomycin (10,000 U/ml, #15140122, Gibco), 1 mM sodium pyruvate (100 mM ...
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bioRxiv - Neuroscience 2024Quote: ... and Glutamax-1 (100X, #35050-061, Gibco). The homogenous cells were seeded onto poly-L-lysine-coated coverslip in 12-well plates or 6-well plates ...
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bioRxiv - Neuroscience 2024Quote: ... The dissected cortices were then transferred to a 15 ml tube and washed three times using dissection buffer consisting of HBSS (10X, #14185052, Gibco), 100 U/ml Penicillin/streptomycin (10,000 U/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The cortices were incubated in a dissection buffer containing 0.03% trypsin-EDTA (0.25%, #25200056, Gibco) and 50 U/ml DNase (10,000 U ...
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bioRxiv - Neuroscience 2024Quote: ... sheep anti-parvalbumin (PA5-47693, Invitrogen), goat anti-CD31 (AF3628 ...
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bioRxiv - Neuroscience 2024Quote: ... and quantitative PCR was performed using Fast SYBR™ Green Master Mix Kit (Applied Biosystems, #4385612). Gapdh was used as control to normalize the expression levels of analyzed gene transcripts ...
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bioRxiv - Neuroscience 2024Quote: ... and NOD2−/− primary microglia treated with M18-MDP (1 μg/ml) or α-Syn PFF (5 μg/ml) were lysed in ubiquitination lysis buffer (#PI89900, ThermoFisher), supplemented with protease inhibitor ...
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bioRxiv - Neuroscience 2024Quote: ... 0.25% triton X-100 and 1XTBS at 4°C followed by immunostaining using goat Alexafluor-labeled secondary antibodies (Thermo Fisher Scientific or Jackson ImmunoResarch Laboraties ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme (Invitrogen, #11766500), and quantitative PCR was performed using Fast SYBR™ Green Master Mix Kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted on glass slides using ProLong Gold (#P36934, Thermo Fisher Scientific) and imaged on a Nikon laser scanning confocal microscope or a Ti2 widefield microscope at the Northwestern University Center for Advanced Microscopy and Nikon Imaging Centre.
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bioRxiv - Neuroscience 2024Quote: ... The total protein content of the lysates was determined by BCA protein assay (#23227, ThermoFisher), and 20 µl of Anti-Ub TUBE agarose resin was added ...
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bioRxiv - Neuroscience 2024Quote: ... and developed using Pierce ECL (enhanced chemiluminesence) (Thermo Fisher Scientific). ECL signals were quantified on a Bio-Rad ChemiDoc MP Imaging System and chemiluminescent signals were quantified using Bio-Rad Image Lab Software (6.1).
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bioRxiv - Neuroscience 2024Quote: ... in phenol red-free Opti-MEM I reduced serum medium (Gibco/Thermo Fisher Scientific) (2 h ...
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bioRxiv - Neuroscience 2024Quote: ... plus TRIzol (Life Technologies, #15596-026). cDNA was synthesized using SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and Halt phosphatase inhibitor (#78420, Thermo Fisher Scientific). Protein concentration was determined using bicinchoninic acid assay (BCA ...
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bioRxiv - Biophysics 2024Quote: ... precleared and immunoprecipitated overnight at 4 °C with 50 μL of GFP-conjugated Dynabeads Protein A (Invitrogen). Beads had previously been conjugated with 10 μL anti-GFP antibody (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... Beads had previously been conjugated with 10 μL anti-GFP antibody (Invitrogen, A6455) for 2 hours at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... or Alexa Fluor 647 dye (AF647, Thermo Fisher Scientific). The labeling ...
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bioRxiv - Biophysics 2024Quote: ... fitted on an Orbitrap Eclipse™ Tribrid (Thermo Scientific). Spray voltage in the Easyspray™ source was set to 2.5 kV ...
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bioRxiv - Biophysics 2024Quote: ... The column outlet was directly connected to an Easyspray™ (Thermo Scientific) fitted on an Orbitrap Eclipse™ Tribrid (Thermo Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... the purified proteins were labeled with DyLight 488 dye (DL488, Thermo Fisher Scientific) or Alexa Fluor 647 dye (AF647 ...
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bioRxiv - Plant Biology 2024Quote: ... Synthesis of cDNA was performed using the Taqman Reverse Transcription Reagents kit (ThermoFisher Scientific). Each qRT-PCR reaction contained cDNA (equivalent of 3 ng RNA ...
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bioRxiv - Plant Biology 2024Quote: ... while its concentration was estimated using a fluorescent DNA intercalating agent (e.g., Quant-iT PicoGreen dsDNA Assay Kit, Thermo Fisher Cat. No. P7589) and a microplate reader (Thermo Fisher Scientific)) ...
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bioRxiv - Plant Biology 2024Quote: ... The final libraries were quantified using PicoGreen (Thermo Fisher Scientific) and equally combined into a single sample ...
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bioRxiv - Plant Biology 2024Quote: ... the cDNA libraries were cleaned up using AMPure XP magnetic beads and concentrations were measured using the Qubit® dsDNA BR Assay kit (ThermoFisher Scientific) using a Qubit® 3.0 Fluorometer.
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bioRxiv - Plant Biology 2024Quote: ... and analyzed in a Step-OnePlus real-time PCR system (Applied Biosystems). The RT-qPCR analyses used two or three biological samples with two technical replicates each ...
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bioRxiv - Plant Biology 2024Quote: ... The quantity and quality of genomic DNA was assessed using a Qubit 3 fluorometer (Thermo Fisher, Waltham, MA, USA) and a Bioanalyzer DNA12000 kit (Agilent ...
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bioRxiv - Plant Biology 2024Quote: ... before being used for cDNA synthesis with SuperScript IV Reverse Transcriptase (Invitrogen). The resulting cDNA was then amplified using Phusion High-Fidelity DNA Polymerase (New England Biolabs) ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from 30 mg of macerated leaf tissue using Trizol according to the manufacturer’s instructions (TRIzol™ Reagent – Invitrogen). RNA quantification was performed with the aid of NanoDropTM Lite (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... LR reactions (Invitrogen) were then used to combine plasmids pENTR L4-promoter-R1 ...
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bioRxiv - Plant Biology 2024Quote: ... RNA quantification was performed with the aid of NanoDropTM Lite (Thermo Scientific, Wilmington, DE) under an absorbance of 260 nm ...
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bioRxiv - Plant Biology 2024Quote: ... and ligated to an RNA adapter following the manufacture’s guide of GeneRacer kit (Invitrogen), the RNA ligation reaction was performed for 5 hours at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... or X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside, W5376C; Thermo Fisher Scientific, Guilford, CT). GUS and/or LacZ-stained tissues were cleared for 30 s with 12 % sodium hypochlorite solution before microscopy observations.
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bioRxiv - Cancer Biology 2024Quote: ... Protein measurement was determined using Pierce™ BCA Protein Assay Kits BCA assay (Thermo Scientific™, Cat# 23225) using Pierce™ Bovine Serum Albumin Standard Pre-Diluted Set (Thermo Scientific™ ...
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bioRxiv - Cancer Biology 2024Quote: ... HK2 Monoclonal Antibody (3D3) (Invitrogen™, Cat# MA5-15679, RRID:AB_10986812) at 1:1,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... using Pierce™ Bovine Serum Albumin Standard Pre-Diluted Set (Thermo Scientific™, Cat# 23208). Samples were run on 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with Protease Inhibitor Cocktail and Halt Phosphatase Cocktail (Thermo Scientific™, Cat# 78440). Protein measurement was determined using Pierce™ BCA Protein Assay Kits BCA assay (Thermo Scientific™ ...
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bioRxiv - Plant Biology 2024Quote: ... This was followed by an overnight incubation at room temperature in the dark with 200 ng/µL WGA Alexa Fluor 488 (Invitrogen W11261) in PBS ...