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Citations for Thermo Fisher :
6001 - 6050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis was performed with the MultiScrib (Applied biosystems, Cat#4368813) followed by a quantitative reverse transcription polymerase chain reaction (qRT-PCR ...
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bioRxiv - Cell Biology 2024Quote: ... for 3 min and 1:500 of Hoechst 33342 (20 mM, Thermo Fisher Scientific) for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... and sodium pyruvate were purchased from Gibco (Burlington, ON, Canada). Fetal bovine serum was obtained from Hyclone (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was done on the StepOnePlus Real-Time PCR System (Applied Biosystems) in a 20 µl reaction volume consisting of the following ...
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bioRxiv - Cell Biology 2024Quote: ... once with 2× SSC and once with wash buffer (10% formamide (Ambion, #AM9342) in 2× SSC) ...
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bioRxiv - Cell Biology 2024Quote: ... and SYBR green master mix (Life Technologies, Cat#11733046). Gene expression was normalized to GAPDH for in vitro systems and Actb for in vivo experiments ...
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bioRxiv - Cell Biology 2024Quote: ... Dissolved AlexaFluor 568 NHS Ester (Invitrogen, Waltham, MA USA) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... washed 3 times with DPBS and blocked with 1mg/mL ultrapure BSA (50 mg/mL, Invitrogen) in DPBS for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a quantitative reverse transcription polymerase chain reaction (qRT-PCR) on an ABI 7900HT cycler (Applied Biosystems) and SYBR green master mix (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... amine-modified DNA oligonucleotides were mixed with 25 μg Alexa Fluor 647 (AF647, Thermo Fisher Scientific) or CF568 (Sigma Aldrich)-conjugated succinimidyl ester in 0.1 M sodium bicarbonate (pH 8.5 ...
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bioRxiv - Cell Biology 2024Quote: ... and cDNA prepared using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). qPCR was done on the StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... RNA concentrations were measured using the Nanodrop 1000 (ThermoFisher). cDNA synthesis was performed with the MultiScrib (Applied biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... was labelled with AlexaFluor568 or AlexaFluro680 according to instructions of the manufacturer (ThermoFisher, Waltham, MA, USA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed once with 1× PBS and stained sequentially with 1:10 dilution of Trypan Blue Solution (0.4%, Thermo Fisher Scientific) for 3 min and 1:500 of Hoechst 33342 (20 mM ...
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bioRxiv - Cell Biology 2024Quote: ... nuclear protein was isolated using the NE-PER nuclear kit (Thermo Scientific), then proteins separated on 4-12% Bis-Tris Bolt gels using electrophoresis before transfer to nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007 ...
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bioRxiv - Cell Biology 2024Quote: ... then proteins separated on 4-12% Bis-Tris Bolt gels using electrophoresis before transfer to nitrocellulose membranes (Thermo Fisher Scientific) by iBlot (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... using Lipofectamine 3000 (Life Technologies). Two days after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... MCF10A and MCF10Am cells were cultured in DMEM:F12 mixture (Gibco) supplemented with 5% horse serum (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were transiently transfected using Lipofectamine 3000 (ThermoFisher Scientific) based on the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2024Quote: ... and U-2OS (ATCC HTB-96) cells were maintained in McCoy’s 5A medium (Gibco). Media was supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: RNA samples generated for RNA-seq experiment were treated with TURBO DNase (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... cells were kept in MEM Alpha Medium supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin (Invitrogen GmbH, Darmstadt, Germany) in a humidified atmosphere at 5% CO2 and 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were incubated with primary antibodies (Rabbit-anti-GFP: 1:1000, Thermo Fisher Scientific, Cat. #G10362; mouse-anti-c-myc: 1:200, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were TXR-anti-rabbit (Invitrogen T-2767), TXR-anti-goat (Invitrogen PA1-28662) ...
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bioRxiv - Cell Biology 2024Quote: ... emiPSCs were stained with custom elephant-specific antibodies for NANOG and OCT4 with TXR-anti-rabbit (Invitrogen T-2767) and SOX2 (Invitrogen MA1-014 ...
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bioRxiv - Cell Biology 2024Quote: Data Analysis was performed using Proteome Discoverer 2.5 (Thermo Scientific) with search engine Sequest ...
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bioRxiv - Cell Biology 2024Quote: ... A 100μL aliquot of Cell Extraction Buffer (Invitrogen) containing 1μL/mL protease inhibitor solution (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... The two supernatants were combined and subjected to high pH fractionation using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: High pH fractions were analyzed using an UltiMate 3000 nano-HPLC system coupled to a Orbitrap Eclipse mass spectrometer (Thermo Scientific, Bremen, Germany). The peptides were separated on a homemade fritless fused-silica microcapillary column (100 µm i.d ...
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bioRxiv - Cell Biology 2024Quote: ... and SOX2 (Invitrogen MA1-014) with AF488-anti-mouse (Invitrogen A-21202) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then stained with PE (Phycoerythrin) mouse/rat anti-CD61 (1:500, Invitrogen; 12-0611-82) and Alexa Fluorβ 647 mouse/human anti-SSEA1 antibodies (1:200 ...
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bioRxiv - Cell Biology 2024Quote: Mouse Gamma Globulin Control (ThermoFisher Scientific 31878)
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bioRxiv - Cell Biology 2024Quote: ... 2×103 EpiLCs per well were seeded in a U-bottom 96-well plate (ThermoFisher Scientific, 268200) in GK15 medium (GMEM with 15% KSR ...
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bioRxiv - Cell Biology 2024Quote: ... single-cell suspensions were stained with 1 μg/ml propidium iodide (PI, Invitrogen; P3566) and filtered through a 70 μm cell strainer ...
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bioRxiv - Cell Biology 2024Quote: ... Apoptotic assays: Apoptotic cells were identified by labeling 3’ ends of DNA using a BrdU apoptosis kit (Fisher Scientific cat. 88-6671-88) and anti-BRDU antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... After 1 week of Geneticin™ (80 μg/ml; Life Technologies, 10131035) selection (all vectors except pPyCAG-PBase contain neomycin resistance genes) ...
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bioRxiv - Cell Biology 2024Quote: cyclin D3 (1:1000, Thermo Fisher Scientific MA5-12717)
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bioRxiv - Cell Biology 2024Quote: ... Zo1 (Thermo Fisher Scientific, 40-2200, 1:100), E-cadherin (1:100) ...
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bioRxiv - Cell Biology 2024Quote: ... and Sox2 (Thermo Fisher Scientific, MA1-014, 1:100). After washing twice with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... and 1% knockout serum replacement (KSR, ThermoFisher, 10828010). The medium was changed the following day ...
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bioRxiv - Cell Biology 2024Quote: ... the slides were incubated at 37 °C for 30 min with a 1:500 dilution of secondary antibody goat anti-rabbit IgG 594 (Molecular Probes, A11012) and/ or goat anti-mouse IgG 488 (Molecular Probes ...
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bioRxiv - Cancer Biology 2024Quote: ... 5% CO2 in RPMI 1640 (Gibco, cat. 11875-093) supplemented with 20% fetal bovine serum (Gemini ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissue fixation was performed with 4 % paraformaldehyde (Thermo Scientific, J19943.K2) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... cells were cultured in phenol red-free DMEM (Thermo Fisher Scientific, 31053028) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% L-glutamine (Gibco, cat. 25030-081), 1% MEM non-essential amino acids (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% MEM non-essential amino acids (Gibco, cat. 11140-050), 0.5% penicillin/streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1× protease/phosphatase inhibitor (Thermo Fisher 78440). Approximately 3 mg of protein lysates were mixed with protein A/G-magnetic beads (Thermo Fisher 88802 ...
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bioRxiv - Cancer Biology 2024Quote: ... donkey anti-mouse Alexa Fluor 568 (Invitrogen; 1:500), and goat anti-rabbit Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5% penicillin/streptomycin (Gibco, cat. 15140-122), 1% sodium pyruvate (Sigma ...