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Citations for Thermo Fisher :
6151 - 6200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... biotin azide (with HPG alkyne) was detected by Streptavidin Alexa Fluor™ 647 conjugate (Invitrogen) and acquired by iBright FL1500 Imaging ...
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bioRxiv - Molecular Biology 2024Quote: ... equipped with a Selectris X energy filter and a Falcon 4i (Thermo Fisher Scientific, USA) direct electron detector ...
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bioRxiv - Molecular Biology 2024Quote: ... Grids were clipped and loaded into a 300-kV Titan Krios G4i microscope (Thermo Fisher Scientific, USA) equipped with a Selectris X energy filter and a Falcon 4i (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... were applied to the freshly glow-discharged grids and plunge-frozen in LN2-cooled liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) with a blotting time of 2.5 s ...
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bioRxiv - Cell Biology 2024Quote: ... was transfected with Lipofectamine™ 3000 Transfection Reagent (Invitrogen). 72 h after the transfection ...
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bioRxiv - Cell Biology 2024Quote: ... and the Click-it reaction between Click-iT® HPG alkyne (present in protein and Alexa Fluor™ 555 Azide, Triethylammonium Salt (Invitrogen) was applied using the Click-iT™ Cell Reaction Buffer Kit (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... pH 8.0) 1x Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific). After incubating on ice for 40 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and 1x Penicillin-Streptomycin (Life Technologies, no. 15140-122). All C3H10T1/2 cells were maintained below passage 15.
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bioRxiv - Cell Biology 2024Quote: ... was applied using the Click-iT™ Cell Reaction Buffer Kit (Invitrogen). Parallel immunostaining (e.g. ...
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bioRxiv - Cell Biology 2024Quote: ... After washing with Phosphate Buffered Saline (DPBS, Gibco), the cells were permeabilized and blocked for 1 h at RT with 10% donkey (GeneTex ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.375 µL of lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, no. 11668027) were thoroughly mixed ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ml MEM amino acids solution 50x (ThermoFisher, ref.no. 11130036), 10 ml MEM non-essential amino acids solution 100x (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... Library concentration and size distribution were quantified by Qubit (Thermo Scientific) and TapeStation (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... the labelled BSA (AlexaFluor555 conjugate, Molecular Probes; Alexa555-BSA-Au, UMC Utrecht) was clarified by centrifugation (750 x g ...
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bioRxiv - Cell Biology 2024Quote: ... Transfection reaction was carried out in opti-MEM medium (GIBCO) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells received daily media changes and were passaged every 4-5 days as aggregates using 0.5 mM EDTA in DPBS (Cat # AM9260G, ThermoFisher Scientific). Cell aggregates were gently scraped off the bottom of the well and re-seeded onto a new Geltrex-coated dish.
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bioRxiv - Cell Biology 2024Quote: ... and washed 2-times with ice-cold 1X DPBS (Gibco Cat. No. 14190-144). Cells were fixed with ice-cold methanol for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Mitochondria were stained with MitoTracker Deep Red 75nM (15 min, 37°C; Thermo Fisher Scientific) or transfection of mCherry-Omp25 ...
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bioRxiv - Cell Biology 2024Quote: Flow cytometry-based detection of early and late apoptosis of cancer cells was conducted using the Annexin-V apoptosis assay kit (Thermofisher Scientific Cat. No. V13241). Staining of cells with Annexin-V and propidium iodide was conducted according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... we used 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH; Thermofisher Scientific Cat. No. T204). Yeast cells were stained with 0.5µM TMA-DPH ...
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bioRxiv - Molecular Biology 2024Quote: ... and in McCoy’s 5A medium (16600-082; Gibco), respectively ...
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bioRxiv - Molecular Biology 2024Quote: U2OS and HCT116 cell lines were cultured in DMEM (11995-065; Gibco) and in McCoy’s 5A medium (16600-082 ...
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bioRxiv - Molecular Biology 2024Quote: ... and: anti-mouse antibody (A11032) or anti-rabbit antibody (A11037) from Molecular Probes for immuno-fluorescence ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the instructions (C10337; Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... TRAIP (HSS129125) from Invitrogen. For RNAi ...
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bioRxiv - Cell Biology 2024Quote: ... For CLEM assays the grids were mounted with ProLong™ Diamond Antifade with DAPI (Life Technologies) between a coverslip and glass slide and imaged using a DMI6000B widefield microscope as described above ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 μl of 5 mg/ml transferrin (Tetramethylrhodamine conjugate, ThermoFisher Scientific) was added ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% fetal bovine serum (GIBCO) and 1x penicillin/streptomycin (Lonza) ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-rabbit and anti-mouse antibodies conjugated to AlexaFluor dyes (Molecular Probes).
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bioRxiv - Cell Biology 2024Quote: ... 0.5 mM dNTPs, 0.25 mM ATP, 2.75% PEG 4000, 6U T4 PNK [NEB], 0.5U T4 DNA Polymerase [Thermo Scientific] and 0.5U Taq DNA polymerase [Bioline]) was prepared and incubated for 20 min at 12°C ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were cultured in DMEM medium (GIBCO), supplemented with 10% fetal bovine serum (GIBCO ...
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bioRxiv - Molecular Biology 2024Quote: ... Electrophoreses were performed using 4–12% NuPAGE Bis-Tris gels (Invitrogen) and semi-dry western blotting on nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... A 35 mm glass bottom dish was used as an imaging chamber (Glass Bottom Dish, Thermo Fisher Scientific). The dish was first cleaned with 100 % EtOH ...
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bioRxiv - Cell Biology 2024Quote: ... gels were placed in water containing DAPI (Thermo Fisher Scientific, 5 mg/ml) at a 1:1000 dilution and incubated for 30 minutes in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... 45 mg Glycoblue (Thermo Fisher), and 20 mM sodium acetate at –80°C for >1 hr ...
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bioRxiv - Cell Biology 2024Quote: ... DAPI (4′,6-diamidino-2-phenylindole, dihydrochloride, 1:10,000, Invitrogen) was used as a DNA counterstain together with the secondary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed and fixed for 15 min at room temperature (RT) using 3% methanol-free formaldehyde solution (Thermo Fisher).
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bioRxiv - Cell Biology 2024Quote: ... treated with DNase I (Invitrogen) and reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystem ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% Fetal Bovine Serum (Thermo Fisher Scientific) on a siliconized glass slide ...
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bioRxiv - Cell Biology 2024Quote: ... the media was changed to FluoroBrite DMEM (Gibco) supplemented with 20% FBS.
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bioRxiv - Cell Biology 2024Quote: ... WAT was fixed and further processed using the automated Epredia Excelsior AS Tissue Processor (ThermoFisher Scientific). Tissues were dehydrated ...
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bioRxiv - Cell Biology 2024Quote: ... The rest of the embryo was placed into a 12-well plate filled with 2 ml ice-cold 0.25 % Trypsin/PBS (diluted from 2.5 % Trypsin, Gibco). The embryos were chopped into small pieces and incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 16 ml phenol-red free RPMI (Gibco, Cat # 11835-063), and 10 ml 2x HBSS (pH 7.2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lysed in RIPA buffer (ThermoFisher, Cat. # 89901) supplemented with protease inhibitors (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... + 50 µM β-Mercaptoethanol (Gibco, Cat. # 31350-010) + 100 U/ml penicillin and streptomycin (Gibco ...
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bioRxiv - Cell Biology 2024Quote: Each library was quantified on Qubit with Qubit® dsDNA HS Assay Kit (Life Technologies) and then ...
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bioRxiv - Cell Biology 2024Quote: ... using DMEM/HAM F12 culture medium (Life Technologies) supplemented with 10% fetal calf serum (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... and penicillin/streptomycin (1%) (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred with the PowerBlotter XL (Invitrogen by ThermoFisher Scientific) onto nitrocellulose membranes (Amersham™ Protran™ Premium 0.2μm ...
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bioRxiv - Cell Biology 2024Quote: ... and quantified using the Qubit high-sensitivity dsDNA assay (Invitrogen). Seq-Well libraries were equimolarly pooled and clustered at 1,25 nM concentration with 10% PhiX on a NovaSeq6000 system using a S2 flow cell with 100 bp v.1.5 chemistry ...