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Citations for Thermo Fisher :
5801 - 5850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The instrument was calibrated using the NativeMark Protein Standard (Thermo Fisher Scientific) immediately prior to measurements ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were measured on a Nanodrop One instrument (Thermo Scientific). 30 µg of each sample were mixed with 0.2 V of 6x sample buffer (350 mM Tris-Cl pH 6.8 ...
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bioRxiv - Biochemistry 2024Quote: ... and supplemented with 10% bovine calf serum (Gibco) at 37°C in a humidified incubator with 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... A pseudo-infusion was performed by injecting 50 µL using a Dionex Ultimate 3000 connected to a Q Exactive Plus mass spectrometer (Thermo Fisher) without the use of a column ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a 30-minute incubation on ice with Fixable Viability Dye (Thermo fisher, # 65-0865-18), mCD45 Monoclonal Antibody (Thermo fisher ...
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bioRxiv - Biochemistry 2024Quote: ... operating in positive ion mode coupled to an EvoSep One (EvoSep, Odense, Denmark) or EASY-nLC 1200 (Thermo Fisher Scientific) liquid chromatography system ...
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bioRxiv - Biochemistry 2024Quote: Total proteome data were collected on Orbitrap Eclipse mass spectrometer (ThermoFisher Scientific) coupled to a Proxeon EASY-nLC 1000 (or 1200 ...
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bioRxiv - Biochemistry 2024Quote: ... LC separations were performed on a capillary column (Acclaim PepMap100 C18, 2 μm, 100 Å, 75 μm i.d. x 50 cm, Thermo Fisher Scientific) at an eluent flow rate of 300 nL/min ...
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bioRxiv - Biochemistry 2024Quote: ... SIRT1 overexpression plasmid was obtained by amplifying the full length of SIRT1 and inserting into the pcDNA3.1 (-) overexpression vector (V795-20, Invitrogen, USA). ShSIRT1 ...
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bioRxiv - Biochemistry 2024Quote: ... all plant NAPstar sequences were subcloned by gateway cloning [64] into pETG10a (Invitrogen, Carlsbad, CA, United States) (Supplementary Table 3).
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bioRxiv - Biochemistry 2024Quote: Analysis was performed on a Q-Exactive Orbitrap high resolution mass spectrometer (HRMS) from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2024Quote: ... Virus generation was performed using the Bac-to-Bac™ expression system (Thermo Fisher Scientific) in Sf9 insect cells ...
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bioRxiv - Biochemistry 2024Quote: HeLa cells (Leibnitz Institute DSMZ; ACC 57) at passage 4-25 were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Ref ...
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bioRxiv - Biochemistry 2024Quote: ... HEK flp-InTm T-RExTM-293 cells (Invitrogen, Darmstadt, Germany) were transfected with this construct using a standard polyethylenimine-based approach ...
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bioRxiv - Biochemistry 2024Quote: ... and inserted into pDONR207 (LIFE Technologies, Carlsbad, CA, United States) (Supplementary Table 1) ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) (Gibco, Ref ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was eluted from SP3 beads using 200 mM EPPS pH 8.5 containing trypsin (ThermoFisher Scientific, 90305R20) and Lys-C (Wako ...
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bioRxiv - Biochemistry 2024Quote: ... ACC 57) at passage 4-25 were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Ref: 41966029, Thermofisher Scientific,Waltham ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 μL were analyzed by an Ultimate 3000 reversed-phase capillary nano liquid chromatography system connected to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). Samples were injected and concentrated on a trap column (PepMap100 C18 ...
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bioRxiv - Biochemistry 2024Quote: ... An Agilent 1260 binary pump coupled with a photodiode array (PDA) detector (ThermoFisher Scientific) was used to separate the peptides ...
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bioRxiv - Biochemistry 2024Quote: ... coupled to a Proxeon EASY-nLC 1000 (or 1200) LC pump (ThermoFisher Scientific). Peptides were separated using a 90-120 minute gradient at 500-550 nL/min on a 30 cm column (i.d ...
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bioRxiv - Biochemistry 2024Quote: ... Mach1 (Invitrogen) and NEBStable (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... MS and MS/MS raw data were processed using Proteome Discoverer version 2.4 (Thermo Fisher Scientific) to generate MGF files as described before28 ...
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bioRxiv - Biochemistry 2024Quote: ... with ∼60 µg of TMTPro 16plex reagents (ThermoFisher Scientific). Following confirmation of satisfactory labelling (>97%) ...
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bioRxiv - Biochemistry 2024Quote: ... in 1 mL of Optimem (11058-021, Gibco). After 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... and 1% v/v penicillin/streptomycin (15070063, Gibco) at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... and the plates were washed with 2 mL of 1x PBS pH 7.4 (10010023, Gibco). The cells were treated with 1 mL of trypsin ...
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bioRxiv - Bioengineering 2024Quote: ... and the concentration was measured using a Nanodrop (Thermo Scientific ND-ONEC-W). To label DO with fluorophores ...
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bioRxiv - Biochemistry 2024Quote: ... hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Biochemistry 2024Quote: ... TMPRSS2 wild-type (WT) and TMPRSS2S441A were cloned into a modified pMT/BiP plasmid (Invitrogen; hereafter termed pT350), which translates the protein in frame with an enterokinase cleavage site and a double strep-tag at the C-terminal end ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were subcultured by trypsinization (25200-056, Gibco). The cell lines were tested for mycoplasma contamination using the LookOut® Mycoplasma PCR Detection Kit (Sigma Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... HisPur cobalt resin beads (Thermo Scientific) were used ...
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bioRxiv - Biochemistry 2024Quote: ... or FBS (A14430-01 Gibco) was used as the base media ...
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bioRxiv - Biochemistry 2024Quote: ... followed by staining with Alexa Fluor 647-conjugated Goat anti-Human antibody (Thermo Fisher Scientific, A-21445, 1:500).
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bioRxiv - Biochemistry 2024Quote: ... The cells were washed with cold 1x PBS (10010023, Gibco) and then harvested in cold 1x PBS ...
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bioRxiv - Biochemistry 2024Quote: ... HisPur cobalt resin beads (Thermo Scientific via Danyel Biotech) were used for protein purification ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were then washed twice with PBS and then incubated with Alexa Fluor 647-conjugated streptavidin (Thermo Fisher Scientific, S21374, 1:400) for 30 min at 4°C.
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bioRxiv - Bioengineering 2024Quote: ... Digested ssDNA product was then mixed with 1 μl 20 mg/mL glycogen (Thermo Scientific, R0561) and 1/3 volume 10 M ammonium acetate to perform EtOH precipitation ...
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bioRxiv - Biochemistry 2024Quote: ... and the buffer was exchanged using Zeba spin desalting columns (7k mwco, ThermoFisher Scientific) at 4°C into different buffers as required for specific experiments.
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bioRxiv - Biochemistry 2024Quote: ... the cell density of each plate was determined using a Countess 3 (Thermofisher). The supernatant was discarded ...
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bioRxiv - Bioengineering 2024Quote: A volume of 4 μl of Cy5-labeled DO structures at 50 nM concentration were mixed with 6 μl R Buffer solution (Neon kits-MPK1096 Thermo Fisher). DO structures in R buffer were then immediately subjected to electroporation using the Neon Transfection system (MPK5000 ...
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bioRxiv - Biochemistry 2024Quote: Mass spectrometry analysis was performed on an Orbitrap Eclipse Tribrid Mass Spectrometer with a High Field Asymmetric Waveform Ion Mobility (FAIMS Pro) Interface (Thermo Scientific) with an UltiMate 3000 Nano Flow Rapid Separation LCnano System (Thermo Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein Thermal Shift Software (ThermoFisher Scientific) was used for data analysis ...
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bioRxiv - Biochemistry 2024Quote: ... Biolog Microbial Phenotyping Microarray Plates PM1-PM6 were resuspended in 125 μL MilliQ water and 5 μL of each well was aliquoted into MicroAmp Optical 96-Well Reaction Plates (ThermoFisher Scientific) to prepare screening plates ...
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bioRxiv - Biochemistry 2024Quote: ... and fractionated using high pH reversed-phase peptide fractionation kits (Thermo Fisher Scientific, P/N 84868) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... cells were given virus production media (DMEM supplemented with 20mM HEPES (Gibco; 15630080) and 30% FBS) ...
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bioRxiv - Biochemistry 2024Quote: ... High Five cells (Thermo Fisher, B85502) were infected with baculovirus at 2.0 x 106 cells/mL and incubated at 27 °C with 120 rpm shaking for 72 h.
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bioRxiv - Biochemistry 2024Quote: ... MG132 and polyethylenimine (PEI) was purchased from Fisher Scientific (Hampton, NH). Lipofectamine 3000 was purchased from ThermoFisher (Waltham ...
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bioRxiv - Biochemistry 2024Quote: RNA oligonucleotides used for binding affinity measurements and crystallographic studies were synthesized on an ABI 394 DNA/RNA synthesizer (Applied Biosystems (ABI); Waltham ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were then loaded on BOLT 4– 12% Bis-Tris gels (Thermo Fisher Scientific) and proteins were separated by SDS-PAGE ...