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Citations for Thermo Fisher :
5901 - 5950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... A High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific) was used to create cDNA from purified RNA ...
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bioRxiv - Biochemistry 2024Quote: ... (Invitrogen #MA3-001); α-Cdk7 (total) ...
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bioRxiv - Biochemistry 2024Quote: ... The peptide samples were sprayed using a nano bore stainless-steel emitter (Fisher Scientific). Peptides were analyzed using an Orbitrap-Exploris-480 MS™ mass spectrometer ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were mixed with 4X NuPage lithium dodecyl sulfate loading buffer (Invitrogen), heated to 90°C for 5 min and then loaded onto 12% NuPAGE Bis-Tris Gels (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein in each sample was quantified by a BCA protein assay (ThermoFisher, Rockford, I L), and boiled with 5×loading buffer (LB ...
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bioRxiv - Biochemistry 2024Quote: ... or DMEM (Gibco) supplemented with 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted using TRIzol (Invitrogen). A High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Total protein concentration was determined using a Pierce BCA assay (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and dried in a DNA 120 SpeedVac Concentrator (Thermo Fisher).
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bioRxiv - Biochemistry 2024Quote: ... and anti-mouse IgG (HRP) (#32430, Thermo Fisher Scientific, Inc., Massachusetts, USA).
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bioRxiv - Biochemistry 2024Quote: ... SYPRO Orange (5000×, Invitrogen) was added at a dilution of 1:1000 (final concentration of 5x) ...
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bioRxiv - Biochemistry 2024Quote: ... Injected sample fractions were preconcentrated and further desalted online using a PepMap C18 nano trap column (2 cm X 75 μm; particle size, 3 μm; pore size, 100 Å; Thermo Fisher) with a flow rate of 3.5 μL/min for 6 min ...
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bioRxiv - Biochemistry 2024Quote: ... Then the supernatant was loaded onto a Ni-NTA-beads (ThermoFisher) batch and washed with 50 mM Tris pH = 8.0 ...
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bioRxiv - Biochemistry 2024Quote: Samples were cleaned up using Sep-Pak cartridges (Waters) and dried in a DNA 120 SpeedVac Concentrator (Thermo Fisher). The dried peptide samples were reconstituted in 50 mM EPPS buffer (pH 8.5) ...
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bioRxiv - Biochemistry 2024Quote: ... Peptide separation was performed using an EASY-Spray C18 reversed-phase nano LC column (Acclaim PepMap RSLC; 50 cm X 75 μm; particle size, 2 μm; pore size, 100 Å; Thermo Fisher) at 55°C and a flow rate of 300 nL/min ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 10% FBS superior (Biochrom) and 100 units/mL penicillin/streptomycin (Gibco). Low-number passages (<10 ...
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bioRxiv - Biochemistry 2024Quote: ... Novex Sharp Pre-Stained Protein Standard (Thermo Fisher) was used as a ladder ...
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bioRxiv - Biochemistry 2024Quote: ... the DMEM was replaced with 100 μL Opti-MEM (Gibco, 31985079) and incubated for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... with 2 wells in MES Running Buffer under reduced conditions at 150 V for 60 min using the XCell SureLock system (Thermo Fisher). 100 µg of protein were loaded onto the gel for each sample ...
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bioRxiv - Biochemistry 2024Quote: Cell viability was assessed using AlamarBlue® reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 1% L-glutamine (Gibco), and 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Biochemistry 2024Quote: The 24 composite fractions were analyzed by LC-MS/MS using a Orbitrap Fusion Lumos mass spectrometer equipped with an EASY Spray Source and connected to an Ultimate 3000 RSLC nano UPLC system (all - Thermo Fisher). Injected sample fractions were preconcentrated and further desalted online using a PepMap C18 nano trap column (2 cm X 75 μm ...
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bioRxiv - Biochemistry 2024Quote: ... TMTpro 16plex reagents (Thermo Fisher) were added at a 5:1 w/w ratio to each sample ...
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bioRxiv - Biochemistry 2024Quote: ... Data were collected on a Titan Krios (Thermo Fisher Scientific, Hillsboro, USA) electron microscope at 300 kV equipped with a K3 camera (Gatan ...
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bioRxiv - Biochemistry 2024Quote: ... The grid was plunged into liquid ethane cooled by liquid nitrogen using Vitrobot Mark IV (Thermo Fisher Scientific). The parameters were set as follows ...
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bioRxiv - Biochemistry 2024Quote: ... connected to a Q-Exactive mass spectrometer (Thermo Fisher Scientific) and separated on a 2.1 mm × 50 mm Acquity UPLC BEH C18 ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC CRL-1573) were seeded in 6-well plates with Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11965092) supplemented with 10 % FBS (Sigma–Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Immune complexes were detected using PierceTM ECL Plus Western Blotting Substrate (Thermo Fisher Scientific 32132) and images were captured using ChemiDocTM MP Imaging System(Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... using lipofectamine 2000 (Invitrogen). Cells were cultured in the transfection media for 6 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed with phosphate buffer saline (PBS) 24 hours post transfection and grown in Earle’s Balanced Salt Solution (EBSS) (24010043, Thermo Fisher) with 10% calf serum for 16 hours to induce amino acid stress.
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bioRxiv - Biochemistry 2024Quote: ... Lipofectamine RNAiMax transfection reagent was from Invitrogen, USA ...
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bioRxiv - Biochemistry 2024Quote: ... in the presence of 10µL liposomes loaded with Dextran-FITC (10kDa, Sigma) (liposomes preparation is detailed in [32]. 4µg of anti-FITC antibody (Rabbit Monoclonal 6HC5LC9; ThermoFisher Scientific) was added to quench external FITC fluorescence ...
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bioRxiv - Biochemistry 2024Quote: ... ECL plus western blotting detection was purchased from Thermo Scientific. Polyethyleneimine (PEI ...
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bioRxiv - Biochemistry 2024Quote: ... preadipocytes were cultured in high-glucose (400 mg/dl) Dulbecco’s modified Eagle’s medium (DMEM, 11965–092, Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: ... Fetal bovine serum (FBS) was purchased from GibcoTM (Life Technologies, Canada). Lipofectamine RNAiMax transfection reagent was from Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: All siRNAs (siControl: Stealth RNAiTM siRNA Negative Control, Med GC, siMat2a #911: MSS232488) were obtained from Invitrogen (Carlsbad, CA, USA). The target sequence of the siMat2a (911 ...
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bioRxiv - Biochemistry 2024Quote: ... methionine and cysteine (Gibco, Carlsbad, USA). L-methionine (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Hepa1 cells were transfected with siRNAs using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: ... Glass cover slips were from Fisher Scientific, Canada.
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bioRxiv - Biochemistry 2024Quote: ... Protein identification using 1200 Easy-nanoLC - Orbitrap Exploris 480 platform (Thermo Fisher Scientific), typically generates up to 1000 protein identifications for similar samples ...
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bioRxiv - Biochemistry 2024Quote: ... All Alexa flour labelled secondary antibodies used for immunocytochemistry were purchased from Molecular Probes, USA ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were observed on a Tecnai F20 FEG electron microscope (FEI, ThermoFisher Scientific) operated at 200 kV using an Eagle 4k_4k camera (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... containing 10% fetal bovine serum (FBS, 26140079, Gibco). For ACSS2 shRNA stable knockdown ...
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bioRxiv - Biochemistry 2024Quote: ... and protein concentration was determined with the Bradford assay (23200, Thermo Fisher). Normalized Lysates were incubated with 20μL of anti-FLAG affinity gel or anti-Acetyl-Lysine (mAb mix ...
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bioRxiv - Biochemistry 2024Quote: HEK293Tcells were cultured in DMEM media (11965–092, Gibco) with 10% calf serum (C8056 ...
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bioRxiv - Biochemistry 2024Quote: ... the liver was perfused with Liver perfusion medium (Thermo Fisher Scientific) at 37℃ for 10 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... or 6% (Thermofisher) tris-glycine SDS-PAGE gels then transferred onto PVDF membranes using the Transblot rapid transfer machine (BioRad) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml streptomycin and 4 mM glutamine (Gibco, Carlsbad, USA) were added in DMEM without glutamine ...
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bioRxiv - Biochemistry 2024Quote: ... Pierce™ BCA Protein Assay Kit and Halt™ Protease and Phosphatase Inhibitor Single-Use Cocktail were from Thermo Scientific™ ...
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bioRxiv - Biochemistry 2024Quote: ... Conditioned media for methionine and cysteine were prepared as follows: 10% dialyzed FBS (Gibco, Carlsbad, USA), 100 U/ml penicillin ...