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Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Complementary DNA was synthesized by High Capacity cDNA Reverse Transcription Kits (Applied Biosystems, Foster city, CA, USA). Quantitative PCR was performed using LightCycler Fast Start DNA Master SYBR Green I ...
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bioRxiv - Biophysics 2024Quote: ... prepolymer solutions of the 3900 kPa native placental stiffness containing 0.5% volume of 0.5 μm diameter carboxylate-modified fluorescent beads in PBS (FluoSpheres, Invitrogen, Catalog: F8812) were prepared as previously described34 ...
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bioRxiv - Biophysics 2024Quote: ... all cell culture materials and supplies were purchased from Fisher Scientific (Ottawa, ON) and chemicals from Sigma Aldrich (Oakville ...
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bioRxiv - Biophysics 2024Quote: HEK293 cells were maintained at 37 °C in 5% CO2 containing Dulbecco’s modified Eagle’s medium (DMEM, purchased from Thermofisher) supplemented with 10% fetal bovine serum and penicillinstreptomycin (0.2 mg/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by 0.05 % Trypsin-EDTA (Gibco) for 4 minutes ...
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bioRxiv - Biophysics 2024Quote: ... Affinity chromatography was performed using Co+2-NTA resin (Thermo Fisher). Washing buffer (50mM sodium phosphate ...
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bioRxiv - Biophysics 2024Quote: ... A fluorescence stain SYPRO Orange dye solution (ThermoFisher) was used and diluted to 5x concentration in 20 mM Hepes and 150 mM NaCl at pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... and the PEG-biotin:streptavidin:FUS-biotin tethering system was established by incubating 1 mg/mL streptavidin (Thermo Fisher) and then 1 µM biotinylated FUS in sample wells for 15 minutes each ...
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bioRxiv - Biophysics 2024Quote: ... in an iBright system (Thermo Fisher).
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bioRxiv - Biophysics 2024Quote: ... and polyadenylation (Thermo Fisher), employing an initial incorporation of 2’-azido-2’-dATP (Jena Biosciences ...
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bioRxiv - Biophysics 2024Quote: ... Frozen grids were clipped into AutoGrids with a milling slot (Thermo Fisher Scientific) to allow milling at shallow grazing angles as described previously (31 ...
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bioRxiv - Biophysics 2024Quote: ... Coverslips were placed on the MP sample stage and a single gasket was filled with 20 μL DPBS (wo/ calcium and magnesium, pH 7.4, ThermoFisher Scientific) to find focus ...
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bioRxiv - Biophysics 2024Quote: CryoEM grids were imaged using 300KeV Titan Krios G3 (ThermoFisher Scientific) transmission electron microscope (Midlands Regional Cryo-EM Facility at the University of Leicester ...
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bioRxiv - Biophysics 2024Quote: ... ended with a disuccinimidyl tartrate (DST, Thermo Fisher) treatment to neutralize any excess amount of reactive amine sites provided by APTES ...
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bioRxiv - Biophysics 2024Quote: Fluorogenic POP substrate Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin (AMC) (HANGZHOU JHECHEM CO LTD) was diluted in PBS to 1-25 µM final concentrations in 96-well plates (ThermoFisher) to measure enzyme activity parameters in real time and as end-point reactions at 10 and 30 min ...
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bioRxiv - Biophysics 2024Quote: ... 20-nm FluoSpheres Carboxylate-Modified Microspheres (Thermo Fisher), 9.5-nm CdSe/ZnS core-shell type QDs functionalized with carboxylic acid ...
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bioRxiv - Biophysics 2024Quote: ... Membrane stain FM5-95 (final concentration of 5 μg/mL; Molecular Probes) and 0.5 mM IPTG were added to the solidified LB ...
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bioRxiv - Biophysics 2024Quote: Tilt series were acquired on the Titan Krios G3 (Thermo Fisher Scientific) at 300 keV with either a K2 detector and Quantum 968 LS post-column energy filter or a K3 Summit detector with 1067HD BioContinuum post-column energy filter in counting and dose fractionation modes (Gatan) ...
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bioRxiv - Biophysics 2024Quote: ... and EPU automated data acquisition software (ThermoFisher Scientific). Micrograph movies were recorded with 75 fractions ...
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bioRxiv - Biophysics 2024Quote: ... prior to flash-cooling in liquid ethane using a Vitrobot Mark IV (FEI ThermoFisher), set at 4 °C and 100% humidity.
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bioRxiv - Biophysics 2024Quote: ... Cryo-FIB milling was performed in an Aquilos Dual-Beam (Thermo Fisher Scientific) as described previously (31 ...
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bioRxiv - Biophysics 2024Quote: ... MaxiSorp flat-bottom 96-well ELISA plates (ThermoFisher) were coated with recombinant TcPOP (2 µg/mL in PBS ...
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bioRxiv - Biophysics 2024Quote: ... An Orbitrap Fusion Lumos Tribrid (Thermo Fisher Scientific) was used to perform online mass spectrometric analyses ...
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bioRxiv - Biophysics 2024Quote: The sample were assessed on the SDS-PAGE gel (Bolt™ Bis-Tris Plus Mini Protein Gels, 4-12% gradient) (Page Ruler Pre-stained Ladder, ThermoFisher) and transferred to nitrocellulose membrane using Trans-Blot Turbo system (Biorad) ...
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bioRxiv - Biophysics 2024Quote: ... followed by the signal detection using SuperSignal West Pico PLUS Chemiluminescent Substrate (Cat. 34580, ThermoFisher) in an iBright system (Thermo Fisher).
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bioRxiv - Biophysics 2024Quote: ... 0.2% (v/v) formic acid and loaded onto an EASY-Spray column (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... via an EASY-nLC 1200 (Thermo Fisher Scientific). The column temperature was set to 35°C ...
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bioRxiv - Biophysics 2024Quote: ... a click-chemistry reaction with AF647 sDIBO alkyne (Thermo Fisher) was conducted ...
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bioRxiv - Biophysics 2024Quote: ... Calibration involved introducing 3 μL of a 1:100 v/v pre-diluted NativeMark standard (LC0725, Thermo Scientific) to an acquisition well ...
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bioRxiv - Biophysics 2024Quote: ... fluorescent beads were purchased from ThermoFisher (F8801 and F8784) and Bangs Laboratories Inc ...
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bioRxiv - Biophysics 2024Quote: ... the protein concentration of the final product was measured on NanoDrop (Thermo Fisher) with an extinction coefficient of 70,140 M-1cm-1 ...
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bioRxiv - Biophysics 2024Quote: ... Nitrocellulose membrane was incubated with His6-tagged Monoclonal Antibody - HRP (Invitrogen) with 1:3000 v/v dilution in PBS-T ...
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bioRxiv - Biophysics 2024Quote: ... The 3 mAbs were specifically conjugated to HRP using EZ-Link™ Plus Activated Peroxidase Kit (ThermoFisher) and detected with ECL (Pierce ...
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bioRxiv - Biophysics 2024Quote: ... The linear PCR product was phosphorylated with T4 Polynucleotide kinase and circularized using T4 DNA-ligase according to manufacturer’s instructions (Thermo Scientific). All constructs were verified by Sanger Sequencing.
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bioRxiv - Biophysics 2024Quote: ... cells were detached using ethylenediaminetetraacetic acid (EDTA) 5 [mM] (ThermoFisher), and subsequently plated in the micropatterns.
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bioRxiv - Cancer Biology 2024Quote: ... CD11c (ThermoFisher Scientific: N418), CD80 (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were seeded in 4 confocal dishes (ThermoFisher Scientific) and grown in DMEM in 5% CO2 in a 37 °C incubator ...
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bioRxiv - Biophysics 2024Quote: ... The dishes were washed once with Dulbecco’s Phosphate-Buffered Saline (DPBS, 1X, Gibco, ThermoFisher) prior to the culture ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 1% Penicillin-Streptomycin (PenStrep, ThermoFisher), on 100 [mm] diameter Corning culture dishes ...
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bioRxiv - Cancer Biology 2024Quote: ... and human gastrointestinal stromal tumour cells (GIST882) (National Infrastructure of Cell Line Resource, Beijing, China) were seeded in confocal dish (ThermoFisher Scientific) and grew in DMEM in 5% CO2 in a 37 °C incubator ...
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bioRxiv - Cancer Biology 2024Quote: ... which were induced into iNSC following manufactured protocol (Gibco, #A1647801).
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bioRxiv - Cancer Biology 2024Quote: ... CD11b (ThermoFisher Scientific: M1/70), IFNγ (BD Biosciences ...
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bioRxiv - Biophysics 2024Quote: ... the cells were passaged by detaching the cells with 3 [mL] of TrypLE Express (with Phenol Red, 1X, Gibco, ThermoFisher) for 2 [min] and then plating 1,5×106cells/[mL] in a new coated Corning culture dishes with 100 [mm] diameter in a total of 10 [mL] new full endothelial growth medium.
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bioRxiv - Biophysics 2024Quote: ... the cells were passaged by detaching the cells with 3 [mL] of TrypLE Express (with Phenol Red, 1X, Gibco, ThermoFisher) for 2 [min] and then plating 1,5×106cells/[mL] in a new coated Corning culture dishes with 100 [mm] diameter in a total of 10 [mL] new full endothelial growth medium.
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bioRxiv - Cancer Biology 2024Quote: The concentrations of purified protein HMGB1a and extracted genomic DNA were determined by NanoDrop OneC (Thermo Fisher Scientific Inc.). The concentrations of whole cell proteins from different cells were determined using a BCA kit (Beyotime Biotech Inc.).
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bioRxiv - Biophysics 2024Quote: ... The dishes were washed once with Dulbecco’s Phosphate-Buffered Saline (DPBS, 1X, Gibco, ThermoFisher) prior to the culture ...
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bioRxiv - Cancer Biology 2024Quote: ... the SunTag plasmids and HMGB1-BFP (2 dishes) or Smad3-BFP plasmid (2 dishes) were simultaneously transfected with Lipo3000 transfection reagent (ThermoFisher Scientific). One dish of cells transfected with HMGB1-BFP ...
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bioRxiv - Biophysics 2024Quote: ... sodium pyruvate (Gibco), MEM NEAA (Non-Essential Amino Acids – Gibco) ...
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bioRxiv - Biophysics 2024Quote: ... and Alexa Fluor 555 Phalloidin (Invitrogen) for microtubules and actin respectively at 1:100 dilutions ...
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bioRxiv - Biophysics 2024Quote: ... A single colony was resuspended in 2 ml LB broth (Fisher Scientific) supplemented with kanamycin and grown overnight at 37°C ...