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Citations for Thermo Fisher :
5851 - 5900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... resuspended in Advanced DMEM/F12 (ThermoFisher #12634010) supplemented with L-glutamine (2 mM ...
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bioRxiv - Cell Biology 2024Quote: ... DPBS supplemented with FCS (10% vol/vol, Gibco, #10270-106) was added to the cell suspension ...
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bioRxiv - Cell Biology 2024Quote: ... The 100 µm capillary column was packed in-house with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). The peptides were separated using a 180 min linear gradient from 5% to 32% buffer B (90% ACN + 0.1% formic acid ...
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bioRxiv - Cell Biology 2024Quote: ... Penicillin-Streptomycin (100 Units/ml; 100 μg/ml respectively, ThermoFisher #15140122), EGF (100 ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... The channels were washed three times with DPBS and chips were submerged in DPBS supplemented with Penicillin-Streptomycin (100 Units/ml; 100 μg/ml respectively, ThermoFisher #15140122) and stored at 4 °C until use for sectioning ...
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bioRxiv - Microbiology 2024Quote: ... we used the Sciex X500R QTOF LC-MS/MS system (Sciex Applied Biosystems, MA, USA) which consisted of an Exion LC liquid chromatography with a C18 column (150×2.1 mm ...
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bioRxiv - Microbiology 2024Quote: ... and fluorescence quantitative analysis was performed using the ABI 7500 PCR system (Applied Biosystems). Conditions were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... the unstained and Zombie Aqua-stained samples were fixed by incubation in PFA (4% vol/vol, ThermoFisher #28908) diluted in DPBS for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... containing 10% FBS and 1% penicillin/streptomycin (Gibco 15140). To induce differentiation to brown adipocytes ...
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bioRxiv - Cell Biology 2024Quote: ... B27 (1x, ThermoFisher #17504044). Media was replaced every other day ...
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bioRxiv - Cell Biology 2024Quote: ... The base media for adipocyte cell culture was DMEM (Gibco 11965092), containing 10% FBS and 1% penicillin/streptomycin (Gibco 15140) ...
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bioRxiv - Neuroscience 2024Quote: ... The right hemisphere of each sample was immersion-fixed in 4% paraformaldehyde (PFA; Fisher Scientific, MA), diluted in 10x phosphate buffered saline for immunohistochemical analysis ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed using a SuperScript™ VILO™ cDNA synthesis kit (Invitrogen). Relative gene expression was determined using custom Taqman® Gene Expression Array Cards (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Synaptosomes were freshly isolated from one half of the cortical tissue from the left hemisphere using Syn-per reagent (Thermo Scientific #87793) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and a QuantStudio™ 7K Flex Real-time PCR System (Applied Biosystems). Samples were run in triplicate across 29 custom array cards and data was normalized to GAPDH using the delta-Ct method ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative PCR (qPCR) was performed using a QuantStudio™ 12K Flex Real-time PCR System (Applied Biosystems) and a QuantStudio™ 7K Flex Real-time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... BV-2 cell samples were run on an EVOSEP liquid chromatography system using the 15 samples per day preset gradient (88min) and were monitored on a Q-Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific). Whereas ...
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bioRxiv - Neuroscience 2024Quote: ... Media was aspirated and collected EBs were resuspended in fresh Neuronal differentiation medium (NDM) containing DMEM/F12 (Gibco), 0.25% penicillin/streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... biotin-labeled anti-rabbit secondary antibody (1:200, Invitrogen, REF 65-6140, LOT WA314056), and an avidin-linked peroxidase complex (AB-HRP kit #PK-4000 ...
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bioRxiv - Neuroscience 2024Quote: ... Pluritest (ThermoFisher Scientific, Waltham, MA) was used to assign a novelty score in which all IPSC lines were highly dissimilar to the non-pluripotent samples ...
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bioRxiv - Neuroscience 2024Quote: ... 0.25% penicillin/streptomycin (Gibco), 1% N2 supplement (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... Purity and quantity of RNA was evaluated using a Nanodrop 2000 (Thermo scientific). A260/280 above 1.3 and A260/230 ≥ 2.0 were considered acceptable purity levels for RNA.
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bioRxiv - Neuroscience 2024Quote: ... 1% N2 supplement (Gibco), 1% NEAA (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... Relative gene expression was determined using custom Taqman® Gene Expression Array Cards (Thermo Fisher Scientific) pre-loaded with the following commercially available Taqman primers ...
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bioRxiv - Neuroscience 2024Quote: ... diluted 1:1000 in PBS: anti-AꞴ polyclonal antibody (Thermo Scientific, MA #44-136); biotinylated Griffonia simplicifolia Lectin I (GSL 1 ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then loaded into a 10 µl electroporation tip (Invitrogen) and electroporated using the Neon Transfection system (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... RNA concentrations were measured using a spectrophotometer (Nanodrop, xxx) and RNA were used for reverse transcription (High-Capacity cDNA Reverse Transcription Kit, Thermo Fisher #4368813). Abundance of genes of interest was quantified using the SYBR Green-based quantification method (FastStart Universal SYBR Green Mastermix ...
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bioRxiv - Cell Biology 2024Quote: ... and digested with 2 mg/mL collagenase II (Life Technologies) and 15 kU/mL DNAse I (PanReac AppliChem ...
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bioRxiv - Cell Biology 2024Quote: ... Gel was stained with the SilverQuest Silver Staining Kit (Thermo Fisher) to identify differential bands comparing sin-lncRNA and control RNA samples ...
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bioRxiv - Cell Biology 2024Quote: ... ∼250,000 cells were centrifuged at 100 rcf for 3 min and resuspended in 12 µl of electroporation buffer (Invitrogen). 50-100 ng of plasmid DNA were added to the cell suspension ...
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bioRxiv - Cell Biology 2024Quote: ... Sonicated chromatin was incubated overnight with 6 μg of C/EBPβ or IgG antibody (listed in Table 1) followed by 2h incubation with Dynabeads Protein G beads (Invitrogen). After five washes with LiCl buffer (100mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were precleared with Dynabeads Protein A (Invitrogen) for 11h ...
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bioRxiv - Cell Biology 2024Quote: ... and washed once with 1 ml of PBS (Gibco). After analysis with an automated cell counter (Nexcelom) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated overnight at 37°C in X-gal staining solution (1mg/ml X-gal [Thermo Fisher], 0.12 mM K3Fe(CN)6 [Merk] ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were trypsinized with TrypLE (Gibco) for 10-15 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and electroporated using the Neon Transfection system (Invitrogen) with these parameters ...
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bioRxiv - Cell Biology 2024Quote: ... The SMART RT reaction was performed at 42°C for 90□min using commercial SuperScript II (Invitrogen) and a TSO ...
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bioRxiv - Cell Biology 2024Quote: ... and retrieved proteins were loaded in a NuPAGE 4%–12% bis-Tris gel (Invitrogen). Gel was stained with the SilverQuest Silver Staining Kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... 1% penicillin-streptomycin (all Life Technologies/Life Technologies), 10% FCS (Biochrom) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were maintained in DMEM/F12 media (ATCC) supplemented with 10% FBS (ATCC) and 0.01 mg/ml Hygromycin B (Invitrogen) at 37°C and 5% CO2.
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bioRxiv - Cell Biology 2024Quote: ... respectively (Applied Biosystems). Biotinylated RNAs were treated with RNase-free DNase I (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... 1% penicillin-streptomycin (all Life Technologies/Life Technologies), 10% FCS (Biochrom) ...
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bioRxiv - Neuroscience 2024Quote: ... HEK-293 cell samples were run on an EVOSEP liquid chromatography system using the 30 samples per day preset gradient (44 min) and were monitored on a Q-Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific). The mass spectrometer cycle was programmed to collect one full MS scan followed by 20 data dependent MS/MS scans ...
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bioRxiv - Neuroscience 2024Quote: ... HEK-293FT (Invitrogen) cells were maintained in complete medium (4.5g/L Glucose and L-Glutamine containing DMEM supplemented with 10% FBS and 1% Pen-Strep ...
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bioRxiv - Cell Biology 2024Quote: ... 500 μL of glacial acetic acid (ACROS Organics AC124040010; Thermo Fisher) was added to a solution of 3-(Trimethoxysilyl ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Flour 488 (#A11055, Invitrogen). Nuclei were stained with Hoechst 34580 (#H21486 ...
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bioRxiv - Cell Biology 2024Quote: ... The library concentration was quantified by Qubit™ dsDNA Assay kit (Thermo Fisher Cat#Q32851) and library size was determined using D1000 ScreenTape (Agilent Cat# 5067-5582 ...
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bioRxiv - Cell Biology 2024Quote: ... Colony dishes were scanned using EVOSTM FLTM auto imaging system (Thermo Fisher Scientific) every 7 days for 4 passages (day 7 ...
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bioRxiv - Cell Biology 2024Quote: ... The negatively selected fraction of PBMCs was cryopreserved in FBS (ThermoFisher Scientific) with 10% dimethylsulfoxide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... we used the Gateway cloning system (Thermo Fisher Scientific, cat. No. K202020) via an existing plasmid containing the UAS-Brp-Short sequence101 followed by a Gateway cassette ...