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Citations for Thermo Fisher :
6101 - 6150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and dissected adult tissues were extracted in 400 μl Trizol reagent (Invitrogen, Carlsbad, USA) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Each oligonucleotide barcode was rehydrated in 10 µl nuclease-free water (Life Technologies, AM9937) and 210 μl of conjugation solution was added ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were fixed in methacarn (60% MeOH (Fisher Scientific A4124), 30% Chloroform (Fisher Scientific C298) ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were incubated with the reporters for 5 minutes in the dark and then washed with the screening buffer several times before mounting on a slide using Fluoroshield mounting medium (Invitrogen, 00495802).
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bioRxiv - Cell Biology 2024Quote: ... and 10% Acetic acid (Fisher Scientific A38-212)) for 20 min ...
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bioRxiv - Developmental Biology 2024Quote: ... were mounted on the slides using Fluoroshield mounting medium (Invitrogen, 00495802).
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bioRxiv - Developmental Biology 2024Quote: Reporters were diluted in reporter stock solution composed of nuclease-free water (Life Technologies, AM9937), 10x PhenoCycler buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Freestyle CHO-S cells (Thermo Fisher Scientific, UK) were cultured in BalanCD CHO Growth A medium (Irvine Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 30% Chloroform (Fisher Scientific C298), and 10% Acetic acid (Fisher Scientific A38-212) ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by fixation in 4% PFA (diluted from 16% paraformaldehyde solution (Fisher Scientific) on ice for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... 2% sodium pyruvate (both from Gibco), 35 ng/ml IL-4 and 50 ng/ml GM-CSF (both from PeproTech) ...
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bioRxiv - Cell Biology 2024Quote: ... and reverse transcription of RNA was performed using SuperScript VILO cDNA synthesis kit (Invitrogen), according to the manufacturerś instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor™ 647 Conjugate (IB4, ThermoFisher, cat. #I32450) or 25 ug/mL Fluorescein or Rhodamine Lens Culinaris Agglutinin (LCA ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were fixed and permeabilized for 30 minutes at room temperature using the Foxp3 / Transcription Factor Staining Buffer Set (Life Technologies Corp., cat. 00-5523-00), followed by an Fc receptor block (BD Pharmingen ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained with Live/Dead Fixable Aqua (ThermoFisher, cat. L10119) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in Dulbecco’s modified Eagle’s medium (DMEM;Thermo Fisher Scientific, 11965118) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 150,000 KPARG12C or 400,000 3LL-ΛNRAS cells were mixed 1:1 with Geltrex LDEV-Free Reduced Growth Factor matrix (Thermo Fisher Scientific) and injected subcutaneously into one flank ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were dehydrated with increasing concentrations of ethanol and mounted with DAPI ProLong Gold Antifade Reagent (Invitrogen). The following primary antibodies were used ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated with the following primary antibodies for 45 minutes at room temperature: anti-ErbB2 (1:400 dilution; ThermoFisher Scientific, cat. MA5-15050, SF23975824), anti-Ki67 (1:200 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were stacked for 30 min at 60V and resolved for 60 min at 150V in 1X NuPAGE™ MOPS SDS running buffer (Invitrogen) and transferred onto 0.45µm PVDF membrane (Immobilon ...
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bioRxiv - Molecular Biology 2024Quote: ... and loaded onto 4 to 12% Bis-Tris mini protein gels (NuPAGE™, Invitrogen, Waltham, MA, USA, 1.5 mm). PageRuler Plus™ Prestained Protein Ladder (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... was used to adjust the pH of the supernatant (initial pH 6.8 - 7.4) to 4.6 using a portable pH meter (Southern Labware, Fisher Scientific, Hampton, NH, USA). Acetic acid was added to samples ≤1 µL at a time ...
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bioRxiv - Molecular Biology 2024Quote: SW480 and MCF7 cells (western blot positive controls) were cultured in DMEM high glucose medium (Gibco, Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed in radioimmunoprecipitation assay-sodium dodecyl sulfate (RIPA-SDS) and protein concentration was quantified using a bicinchoninic acid (BCA) reagent kit (Thermo Fisher, Waltham, MA, USA) following manufacturer instructions ahead of western blotting.
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS, Cytiva HyClone™, Marlborough, MA, USA) and 1% penicillin/streptomycin (Gibco), and grown at 37 °C in a humidified incubator with 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... PageRuler Plus™ Prestained Protein Ladder (Thermo Fisher, 10 – 250 kDa) was resolved as a molecular weight reference ...
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bioRxiv - Molecular Biology 2024Quote: SW480 and MCF7 cells (western blot positive controls) were cultured in DMEM high glucose medium (Gibco, Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... EV preparations were fixed in s 4% paraformaldehyde (PFA) solution (Thermo Scientific). Five microliters of fixed EVs were deposited onto glow discharged (60 secs 15 mA ...
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bioRxiv - Molecular Biology 2024Quote: ... via a wet transfer system with 1X NuPAGETM transfer buffer (Invitrogen) at 100 V for 60 min ...
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bioRxiv - Molecular Biology 2024Quote: ... or RNAqueous™-Micro Total RNA Isolation Kit (Invitrogen). High-capacity cDNA Reverse Transcription Kit (Applied Biosystems™ ...
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bioRxiv - Cell Biology 2024Quote: ... 200 μg of total protein in a maximum volume of 50 μL was used for the Click-it reaction between Click-iT® HPG alkyne and Biotin Azide (PEG4 carboxamide-6-Azidohexanyl Biotin) (Invitrogen) was performed using Click-iT™ Protein Reaction Buffer Kit (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... Stbl3 one shot competent cells (Invitrogen, no. C737303) were then transformed with pEXPRESS and grown on ampicillin (100 ug/mL ...
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bioRxiv - Cell Biology 2024Quote: ... and the coverslips mounted in ProLong® Diamond Antifade Reagent with DAPI (P36962, Thermo Fisher) or in the case of microplates in Mounting medium with DAPI (ibidi) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were mounted with mounting medium ProLong™ Diamond AntifadeMountan with DAPI (Life technologies) and covered with a #1.5 coverslip ...
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bioRxiv - Cell Biology 2024Quote: ... filtered using 40 µm strainers and counted with Trypan blue live-dead stain (Thermo Fisher Scientific, no. T10282) using a Countess automated cell counter (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred to Low-Fluorescence PVDF Transfer Membranes (Thermo Scientific) and blocked in 3% BSA in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... with 12 mm #1.5 coverslips (Thermo Fisher Scientific), or on 96-well black microplates (Ibidi) ...
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bioRxiv - Cell Biology 2024Quote: ... pyruvate (Gibco), GlutaMAX (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... enteroids were pelleted and washed one time with 1X PBS and fixed in 4% (w/v) PFA (Fisher Scientific) for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Enteroids were pelleted and mounted using Molecular Probes Prolong™ Gold Antifade Mountant (Thermo Fisher) and sealed with CoverGrip Coverslip sealant (Biotium ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 600 nM DAPI (Thermo Fisher) for 10 min at RT in the dark ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with 165 nM Phalloidin (Fisher Scientific) for 40 min ...
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bioRxiv - Cell Biology 2024Quote: ... CCL-226) cells were maintained in basic culture medium containing high-glucose DMEM with GlutaMax and pyruvate (Thermo Fisher Scientific, no. 10569010), 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... or SuperSignal®West Dura (Thermo Scientific), and acquired and quantified using an iBright FL1500 Imaging System.
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bioRxiv - Cell Biology 2024Quote: ... and then for 3-4 h with 50 μM Click-iT® HPG (L-Homopropargylglycine) (Invitrogen). For gel electrophoresis analysis ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µL of Opti-MEM (Thermo Fisher Scientific, no. 31985070) and 0.375 µL of lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... for cell viability and Hoechst 5 µg/ml (Thermo Fisher Scientific, no. H3570) for nuclei ...
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bioRxiv - Cell Biology 2024Quote: ... and time-lapse imaging was performed in FluoroBrite™ DMEM (Gibco) (with or without Hoechst 1 μg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... using a Countess automated cell counter (Invitrogen, no. AMQAX2000).
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bioRxiv - Cell Biology 2024Quote: ... permeabilized with PBS and triton (0.3%, Applichem, no. A4975.0100) and stained with fluorescence dyes: Bodipy 10 µg/ml (boron-dipyrromethene; Invitrogen, no. D3922) for lipids and DAPI (1:5000 ...