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Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Protein identification was done using Proteome Discoverer (Thermo Fisher Scientific) software ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was quantified using the Pierce Gold Standard BCA kit (Thermofisher). 10-30 μg of samples were run on 4-15% (BioRad) ...
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bioRxiv - Biochemistry 2024Quote: ... and peptide concentration was determined using a NanoDrop 2000 spectrometer (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Biochemistry 2024Quote: ... containing 100 U/mL penicillin (Invitrogen), and 100 mg/mL streptomycin (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: Tandem mass tags 10-plex reagents (Thermo Fisher Scientific, San Jose, USA) were utilized to label the resulting peptide samples with the temperature points representing different mass tags ...
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bioRxiv - Biochemistry 2024Quote: ... Quantitative PCR analyses with SYBR Green PCR Master Mix (Applied Biosystems) were done on a BioRad CFX-96 Touch Real-Time PCR Detection System controlled by CFX Maestro (ver2.2 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was quantified with the BCA Protein Assay Kit (ThermoFisher Pierce) and 20µg of protein/sample was loaded per lane on SDS-PAGE gel ...
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bioRxiv - Biochemistry 2024Quote: TetraSpeck microspheres (ThermoFisher T7279 0.1µm) suspended in 0.1% gelatin were plated on a glass-bottom Fluorodish ...
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bioRxiv - Biochemistry 2024Quote: ... The movies were acquired using a Titan Krios G3i microscope (Thermo Fisher Scientific) operated at 300 kV accelerating voltage ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were incubated with 100 nM Hoechst reagent (H-1399, Invitrogen) for 5 min ...
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bioRxiv - Biochemistry 2024Quote: Peptide constructs were co-transfected into HeLa cells with an mCherry-EB1 construct using Lipofectamine 3000 regent following manufacturer’s instructions (Invitrogen #L3000015). mCherry-EB1-8 was a gift from Michael Davidson (Addgene plasmid #55035 ...
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bioRxiv - Biochemistry 2024Quote: ... samples were loaded on Pre-cast 4-20% gradient Tris-Glycine gels (ThermoFisher Scientific) and the gel run in Tris-Glycine Native running buffer (2.5 mM Tris Base ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were treated with 100 µg/mL Proteinase K (AM2546, Invitrogen) for 20 min at 60 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Total protein extracts and aggregates were run on 4-12 % NuPAGE Bis-Tris gels (Life Technologies) in MES-SDS running buffer and gels were stained with AzureRed Fluorescent Protein Stain (Azure Biosystems) ...
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bioRxiv - Biochemistry 2024Quote: ... with a Pierce protease inhibitor EDTA-free tablet (Thermo Scientific) and traces of DNaseI (Sigma)) ...
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bioRxiv - Biochemistry 2024Quote: ... All samples were quantified with the bicinchoninic acid (BCA) assay kit (Thermo Scientific). Lysates were mixed with 5x sample buffer (312 mM Tris-Base ...
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bioRxiv - Biochemistry 2024Quote: ... and plunge-frozen into liquid ethane by a Vitrobot Mark IV (Thermo Fisher Scientific) using the following parameters ...
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bioRxiv - Biochemistry 2024Quote: ... or anti-β-Tubulin (480011 Invitrogen;1:10,000). Secondary antibodies used were either anti-rabbit (170-6515 Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were reverse transcribed using SuperScript III (18080093, Invitrogen). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... a secondary Alexa Fluor CF680 anti-mouse antibody (A-21057 Invitrogen 1:1000) was used instead ...
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bioRxiv - Biochemistry 2024Quote: ... Extracts were chilled on ice and sonicated eight times with 50 % amplitude and duty cycle 50 % with a FisherBrand 120 tip sonicator (Thermo Fisher). Cell debris was pelleted by centrifugation at 200 g ...
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bioRxiv - Biochemistry 2024Quote: ... Then 5 g of HP20 resins (Thermo Fisher) was added to the reaction mixture to absorb the desired product ...
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bioRxiv - Biochemistry 2024Quote: ... Data were collected on a G1 Titan Krios microscope (Thermo Fisher Scientific) with S-FEG as electron source operated at an accelerating voltage of 300 kV ...
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bioRxiv - Biochemistry 2024Quote: ... The protein concentration was estimated by the NanoDropTM Lite Spectrophotometer (Thermo Fisher). The samples were subsequently mixed with loading dye and incubated for 5 min at 95 °C.
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bioRxiv - Biochemistry 2024Quote: ... DSBU (ThermoFisher, A35459), Glutaraldehyde (ThermoFisher) ...
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bioRxiv - Biochemistry 2024Quote: ... samples were run on Bolt 4–12% SDS-polyacrylamide and native PAGE 4-16% gels (Invitrogen) respectively ...
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bioRxiv - Biochemistry 2024Quote: ... Automated data collection was performed using EPU (Thermo Fisher Scientific) with periodic centring of zero loss peak ...
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bioRxiv - Biochemistry 2024Quote: ... The cell suspension was lysed by sonication (FB505 sonicator, Fisher Scientific) on ice for 50 seconds in 5 s on/15-s off cycles at 30% power ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was incubated in the humidity chamber of a Mark III vitrobot (Thermo Fisher) at 20°C and 100% humidity for 30 seconds ...
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bioRxiv - Biochemistry 2024Quote: ... and were flash frozen in liquid ethane using the Vitrobot mark IV (Thermo Fisher Scientific) set at 100% humidity and 4 °C for the prep chamber ...
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bioRxiv - Biochemistry 2024Quote: ... Goat anti-mouse HRP-coupled secondary antibody (Invitrogen, 31444) were from commercial suppliers.
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bioRxiv - Biochemistry 2024Quote: ... Both cell lines were cultured in DMEM (Gibco) containing 100 U/mL penicillin (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein purification was conducted with HisPur Ni-NTA resin (Thermo Scientific) on a Poly-Prep Chromatography Column (BioRad) ...
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bioRxiv - Biochemistry 2024Quote: ... connected to a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher). Solvent A was 0.1% formic acid in water ...
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bioRxiv - Biochemistry 2024Quote: ... sealed by Nunc-sealing tape and incubated at 37°C with constant 600 RPM agitation in a ClarioStar Plus plate reader ...
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bioRxiv - Biochemistry 2024Quote: ... and 100 mg/mL streptomycin (Invitrogen) and supplemented with 10% bovine calf serum (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... These divided wells are then subjected to heating at different temperature blocks (50, 53, and 56°C, respectively) for 4 minutes using a Applied Biosystems Veriti Thermo Cycler (Thermo Fisher Scientific) followed by a ramp down to cool the plate to 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... with an Easy nLC-1200 HPLC (Thermo Fisher), connected to a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... A secondary anti-mouse conjugated to Alexa488 (Invitrogen A11001) diluted at 1:500 in blocking solution was added for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... mCD45 Monoclonal Antibody (Thermo fisher, # 61-0451-82), Mouse Anti-Human CD45 (BD Bioscience ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were buffer exchanged using dialysis (dialysis cassette, 7K MWCO, 3ml, Thermo Fisher). The terminal buffers were 50 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in fresh complete M199 and incubated with 100 nM of MitoTracker DeepRed (MTDR) (M22426; Thermo Scientific) for 30 min ...
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bioRxiv - Biochemistry 2024Quote: All samples were analyzed using a Q-Exactive HF-X or Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) operating in positive ion mode coupled to an EvoSep One (EvoSep ...
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bioRxiv - Biochemistry 2024Quote: ... nuclei and kinetoplasts were stained using Hoechst (Invitrogen H3570) at 2 μg/mL in PBS for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... using Mix PowerUp SYBR Green (Thermo Fisher Scientific) and primers listed in Supplementary Table 1.
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bioRxiv - Biochemistry 2024Quote: ... All RT-qPCR reactions were performed in the platform abi 7500 (Thermo Fisher Scientific) using Mix PowerUp SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... and reducing agent (ThermoFisher: NP0009). Samples were heated at 100°C for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were further lysed via sonication (10 sec on 5 sec off, repeat 3x, ThermoFisher: FB120, 4°C) and protein was clarified by centrifugation at 21,000 x g for 5 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: The mRNA preparation involved the utilization of a T7 mMESSAGE mMACHINE kit (Invitrogen). We amplified the cDNAs corresponding to GRIT ...
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bioRxiv - Biochemistry 2024Quote: ... and 1% Penicillin/Streptomycin (P/S, Gibco: 15140-122), and used for all studies ...