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Citations for Thermo Fisher :
5951 - 5976 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies diluted in 3% BSA/PBST were incubated with the samples at 37°C for 1 h: goat anti-rat IgG (H+L) (Thermo Fisher Scientific A11006, 1:100), goat anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times with PBS and subjected to conjugated secondary antibody diluted in IFF buffer (anti-Mouse-AF488 A21202 Invitrogen, anti-Rabbit-568 A11036 Invitrogen, anti-Rat-AF647 A78947 Invitrogen) at 1:2000 for 2h at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in 500 μL of primary antibody solution containing rat anti-alpha-tubulin YL1/2 (MA1-80017, Invitrogen, CA, SAD, diluted 1:500) overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti-rabbit secondary antibody conjugated with green Alexa Flour 488 and donkey anti-rat secondary antibody conjugated with green Alexa Flour 488 were purchased from Invitrogen (cat. # A21206 and cat. # A21208). Confocal microscopic images were acquired using a Zeiss 710 laser scanning confocal microscope and the associated image acquisition and processing software Zen.
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bioRxiv - Neuroscience 2024Quote: ... the dissected tissues were incubated for 24 hours in secondary goat anti-rat IgG antibodies: Alexa Fluor 488 conjugated (Molecular Probes, Invitrogen, Cat# A11006, RRID: AB_141373) at a final dilution 1:40.
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bioRxiv - Cancer Biology 2024Quote: ... The sample was then washed three times followed by an incubation for 1 h with the following secondary antibodies: goat anti-rat Alexa Fluor 647 antibody (Thermo Fisher, #A78947, 1 µg/ml), goat anti-mouse Alexa Flour 488 antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... brain sections were washed in PBS-T 3×5min and incubated with the corresponding anti-rabbit or anti-mouse Alexa Fluor 568-conjugated and anti-rat Alexa Fluor 488-conjugated IgG secondary antibody (all 1:1000, Thermo Fisher Scientific, Waltham, MA, USA) for 2 hours at RT ...
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bioRxiv - Immunology 2021Quote: Tendon samples were homogenized for 45 seconds at 4 m/s in 600 μL TRIzol reagent using a Fisherbrand™ Bead Mill 4 Homogenizer (Thermo Fisher Scientific, 15-340-164) with reinforced 2 mL tubes (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Coronal sections were cut at 8 μm in thickness and mounted onto charged slides (Fisher brand™ Superfrost™ Plus Microscope Slides, Cat. No. 12-550-15, Thermo Fisher Scientific, Pittsburgh, PA). Non-specific binding was blocked with 10% normal donkey serum (Cat ...
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bioRxiv - Microbiology 2022Quote: ... we used the following antibodies: α-complement C3 (primary: mAb 11H9, ThermoFisher catalog no. MA1-40046; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α-surfactant protein D (primary ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were stainied with secondary antibodies in 0.3% triton-X100/PBS for 2 hours at room temperature (anti-rat Alexa Fluor 568, Thermo Fisher A-11077, 1:500, anti-rabbit Alexa Fluor 488, Thermo Fisher A-11008, 1:500). After three washes in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... for 20 minutes followed by overnight incubation in primary antibodies at 4°C (mouse anti-GM-130, BD Transduction laboratories #610822 1:250; rat anti-VE-cad BV13, ThermoFisher Scientific #14-1441-82 1:500) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S; Rat, Cell Signaling, cat #7077S; Sheep, Thermo Fisher, cat #A16041) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Lung supernatant and serum cytokines were measured using a ProcartaPlex Multiplex Immunoassay kit: Th Complete 14-Plex Rat ProcartaPlex Panel (Thermo Fisher Scientific Cat. EPX140-30120-901) per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were warmed for 1 hour at room temperature before washing for 15 minutes in 1x PBS and incubating for 90 minutes at room temperature in the secondary antibody solution consisting of a mixture of anti-rabbit Alexa Fluor® (488)with anti-rat Alexa Fluor® 568 and/or anti-guinea pig Alexa Fluor® 568(Invitrogen, Thermo Fisher Scientific) diluted in 1x PBS (1:600) ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were washed three times in 1xTBS and incubated in fluorescent Alexa-Fluor-labelled IgG secondary antibodies (Alexa-Fluor goat anti-rabbit 488 Invitrogen A-11008, goat anti-rat 546 Invitrogen A-11081 all 1:400 dilution) in 10% serum solution in 2% TBS-T for 2 h ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated for 2 hours at room temperature in the dark in goat anti-mouse IgG conjugated with Alexa-Fluor 488 or goat anti-rat IgG conjugated with Alexa-Fluor 488 (Molecular Probes, Life Technologies Corporation, Carlsbad, California, USA) diluted 1:100 in KPBS ...
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bioRxiv - Immunology 2023Quote: ... the sections were washed three times and incubated with the secondary antibodies (goat anti-rat IgG (H+L) Cross Adsorbed secondary antibody Alexa Fluor546 (Invitrogen, cat. no. A-11081, Karlsruhe, Germany)) for 1 hr at RT ...
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bioRxiv - Immunology 2023Quote: ... Samples were washed with PBS-T three times (flush×1 and 10 min×2) and stained with Donkey anti-rat IgG Alexa Fluor 488 (Thermo Fisher Scientific, A21208, 1:1000 dilution) and Hoechst 33342 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Transient knockdown of NaV1.7 mRNA was done using short interfering RNA (siRNA) targeting both mouse and rat channel transcripts (Cat# 4390771, ID: s134909; Thermo Fisher Scientific Ambion, Waltham, MA, USA). Permanent knockout of endogenous NaV1.7 in ND7/23 cells was achieved using CRISPR/Cas9 genome editing with plasmid pD1301-AD (pCMV-Cas9-2A-GFP ...
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bioRxiv - Bioengineering 2020Quote: ... The solution pH was adjusted to 7.4 by dropwise additions of 0.1 M HCl or NaOH solution and measured with a pH meter (Model 13-641-253, Thermo Scientific Orion 720A+, Thermo Fisher Scientific, Inc., Waltham, MA, USA). The protein UF experiments were conducted with protein feed solutions at room temperature (25 °C).
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bioRxiv - Cell Biology 2023Quote: ... 96-well ELISA plates were coated with coating buffer (Na2CO3 0.1 M pH 9.6) containing IL-12p70 coating antibody (Invitrogen, clone 20C2 M122 and used at 3 μg/ml) overnight and at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The sections were washed three times with PBS and then incubated with primary antibody anti-CD31 (Rat Anti-Mouse, Thermo Fisher Scientific Inc., cat. no. MA1-40074) with a 1:200 dilution in blocking buffer at 4 °C overnight ...
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bioRxiv - Genomics 2023Quote: ... followed by overnight incubation at 4 °C in a primary antibody cocktail (Rabbit anti-NKX2.1, Abcam ab76013 at 1:200; Rat anti-SOX2, Thermo Fisher Scientific 14-9811-80 at 1:100). After washes with TBS-T ...
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bioRxiv - Neuroscience 2023Quote: The brains of P9-11 C57 mice were immersed and fixed for 3 days in cold 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide HCl (EDC, Thermo Fisher Scientific, in 0.1 M PB, pH 7.4) at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend]; goat anti-myeloperoxidase [goatMPO, AF3667, R&D Systems], and [hamsteranti-CD31, 2H8, MA3105, Thermo Fisher] all diluted 1:200 in blocking buffer), for 3 days at 4°C on a shaker ...