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Citations for Thermo Fisher :
5501 - 5550 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: CIL in single cells encountering cadherin coated beads used cdh3-coated beads prepared by binding purified cdh3-fc (gifted from Dr. Douglas W. DeSimone and Dr. Barry. M. Gumbiner) to Protein A/G coated beads (53132, Thermo Fisher Scientific) (Chappuis-Flament et al. ...
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bioRxiv - Plant Biology 2019Quote: ... The run-on assay was performed in a reaction containing 10 µl 10X transcription assay buffer (500 mM pH7.5 Tris-HCl, 50 mM MgCl2, 1.5 M KCl, 1% sarkosyl, 20 U/ml RNase inhibitor [Invitrogen] and 100 mM DTT), 50 µl nuclei in storage buffer ...
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bioRxiv - Neuroscience 2019Quote: ... forebrains were homogenized in 4 ml 0.32 M HEPES-buffered sucrose solution (0.32 M sucrose, 4 mM HEPES, 0.25 mM PMSF and protease and phosphatase inhibitors (Thermo Fisher Scientific, Cat# 78442)) at 4 °C by using a motor-driven glass-Teflon tissue homogenizer ...
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bioRxiv - Developmental Biology 2020Quote: ... was captured using a GFP binding protein (GBP) covalently conjugated to carboxylic acid decorated Dynabeads (Dynabeads M-270 carboxylic acid, Thermo Fisher Scientific). Untagged SMO (Fig ...
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bioRxiv - Biochemistry 2021Quote: ... IP were performed by incubation of lysates 4 h at 4 °C on a rotating wheel with either 50 µL of DynaBeads M-280 protein A magnetic beads (Thermo Fisher Scientific), pre-loaded with 8 µg of rabbit control (Dako ...
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bioRxiv - Biochemistry 2021Quote: ... The extracts were then complemented to 600 µL with IPD buffer and incubated for 2 h at 4 °C on a rotating wheel with 60 µL of streptavidin-coupled magnetic beads (Dynabeads M-280, Thermo Fisher Scientific). The beads ...
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bioRxiv - Biochemistry 2021Quote: ... 10 µL of protein mix were taken as an input before adding 10 µL of pre-equilibrated magnetic Dynabeads M 270 streptavidin beads (Thermo Fisher Scientific) to the reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by separation on 20% 7 M urea SDS-PAGE gel and immunoblot assay with anti-Biotin antibody (Thermo Fisher Scientific, 692033).
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of total RNA was used for cDNA synthesis using the M-MLV reverse Transcriptase Kit (Invitrogen Ltd, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then lysed using 50 µL lysis buffer (20 mM tris, 0.05 M MgCl2) and Quant-iT™ PicoGreen® dsDNA Reagent (ThermoFisher Scientific) was then added to detect the DNA of the lysed remaining cells ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were reconstituted in 0.5 M TEAB and processed following the instruction manual for the TMT kit (Thermo Fisher Scientific, Waltham, USA). The tryptic peptides were fractionated by high pH reverse-phase HPLC using a Thermo Betasil C18 column (5 μm particles ...
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bioRxiv - Immunology 2020Quote: ... Cells from spleen and BMM were washed 3 times with ice-cold PBS and lysed by M-PER™ Mammalian Protein Extraction Reagent (Thermo Scientific) in the presence of protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:1000 in PBS for 15 m at RT and mounted on glass microscope slides (Fisher Scientific, cat n. 1157-2203) using VectaShield Vibrant mounting reagent (2B Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed three times for 10min each in 0.01☐M PBS and incubated in secondary antibody (goat Alexa Anti-Rabbit 488, 1:1,000; ThermoFisher Scientific, #A-11008) for 2☐h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... and GFP (gift from M Anger, IAPG, CAS) in vitro transcription RNA were carried out using mMessage mMachine® kit (Life Technologies) and a poly(A ...
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bioRxiv - Immunology 2021Quote: 5 µm sections were cut from formalin-fixed paraffin-embedded endoscopically-collected colon mucosal tissue blocks onto SuperFrost plus slides (Fisher Scientific 1255015). Slides were baked at 65°C for 2 hours before use ...
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bioRxiv - Genetics 2019Quote: ... The ovarian tissues were homogenized with M-PER Mammalian Protein Extraction Reagent (Pierce Biotechnology, Rockford, IL, USA) supplemented with Halt TM Protease Inhibitor Cocktail (Thermo Fisher Scientific). Following centrifugation (16,000 g ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in 0.05 M Tris-HCl buffer (pH 7.6) containing 5 mg 3-3′ diaminobenzidine (Thermo Scientific, TA-060-HDX) per 10 mL buffer and a final concentration of 0.01% hydrogen peroxide for 15 min at RT ...
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bioRxiv - Biochemistry 2022Quote: ... at a concentration of 0.23 mg/ml in PBS buffer was covalently coupled to 25 mg of Dynabeads™ M-270 Epoxy magnetic beads (ThermoFisher, #14302D), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... 2.2 mM LAP was added to half of the samples and 10 µM 5-ethynyl-2’-deoxyuridine (EdU) solution from a Click-iT Plus EdU Cell Proliferation Kit (cat. no. C10637; Invitrogen, Waltham, MA) was added to the other half according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... HIC1 siGENOME SMART Pool (M-006532-01; Dharmacon/Horizon Discovery, Cambridge, UK) and HIC1 Pre-designed Silencer (AM16708; Ambion/Thermo Fisher Scientific)] ...
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bioRxiv - Genetics 2022Quote: ... with the following changes: the PacBio universal primer was used instead of the Twist universal blockers and Dynabeads M-270 Streptavidin (Invitrogen, CA, USA) were used instead of Twist streptavidin binding beads ...
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bioRxiv - Plant Biology 2022Quote: ... ∼10 pmol of the biotin-labeled probes used in the EMSA experiments mentioned above were first incubated with 10 µl DynabeadsTM M-280 streptavidin magnetic beads (Thermo Fisher Scientific) for 15 min at room temperature in 1 x B&W buffer [5 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Plant Biology 2022Quote: ... The final step of cDNA synthesis comprised the addition of 1 µL of M-MLV Reverse Transcriptase (Invitrogen Catalog Number 28025-013), 1 µL RNasin® Ribonuclease Inhibitor (Promega Part # N211A) ...
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bioRxiv - Neuroscience 2022Quote: Six μm thick sections from tissue blocks of the above-mentioned regions were cut and mounted on superfrost+ glass slides (Thermo Scientific, USA). All sections were stained for pSer129-αSyn (clone EP1536Y) ...
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bioRxiv - Neuroscience 2022Quote: Mice were deeply anesthetized with pentobarbital and perfused transcardially with 0.1 M phosphate-buffered saline (PBS) followed by 4% formaldehyde solution (Thermo Fisher BP531-500) in 0.1 M phosphate buffer (PB) ...
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bioRxiv - Physiology 2022Quote: ... were homogenized by ultrasonication (Q55 Sonicator, Qsonica Inc., USA) in 5 volumes of 1 M perchloric acid (PCA; A228 Fisher Scientific, USA). The samples were neutralized by adding KHCO3 ...
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bioRxiv - Neuroscience 2023Quote: ... The protein pellets were resuspended in 6 M guanidine solution prepared in 100 mM triethylammonium bicarbonate (TEAB) buffer (Thermo Scientific, Cat# 90114). The protein solutions were reduced with 5 mM dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Tetrode tips were immersed in ALEXA 647 fluorophore coupled Hydrazide (A20502 diluted in 0.5 M KCl, Thermo Fisher Scientific GmbH, Dreieich, Germany) to quantify the tetrode position after each experiment ...
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bioRxiv - Neuroscience 2022Quote: ... filled cells were stained with Alexa-633 conjugated Streptavidin (1:1000 in 0.01 M PBS with 10 % NGS and 0.1 % Triton X-100; Thermo Fisher Scientific, Cat# S21375) overnight at 4 °C (while shaking) ...
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bioRxiv - Neuroscience 2022Quote: ... The fixed cultures were washed twice in 0.01 M PBS and incubated overnight at 4 °C with donkey anti-rabbit Alexa488-labeled secondary antibody (1:1000; Invitrogen, Cat# A21206) in 0.01 M PBS with 10% NGS ...
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bioRxiv - Cell Biology 2024Quote: ... we used 0.2 μm diameter carboxylate-modified polystyrene fluorescent microspheres (FluoSpheres, far red/FR λ=660/680, 2% solid diluted in water, F8807, ThermoFisher Scientific) that were activated to promote covalent coupling with primary amines of proteins exposed on the parasite surface ...
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bioRxiv - Bioengineering 2024Quote: ... the HLOs were treated with 35 µL of Visikol® Histo-M™ in a regular 384-well plate (ThermoFisher Scientific; 242757) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... the HLOs were treated with 35 µL of Visikol® Histo-M™ in a regular 384-well plate (Thermofisher Scientific; 242757) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: Knockdown of RORα in human cells was performed using the siGenome SMARTpool Human RORA (#6095) siRNA from the Horizon Discovery “Dharmacon” portfolio (#M-003440-01-005) and Lipofectamine 2000 (Invitrogen, #11668-030). The siRNA stock was diluted to 20 µM in RNase-free water following the product protocols ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed with serum-free RPMI-1640 medium before being transfected with 5 µL of siRNA pool (20 µM) and 5 µL Lipofectamine 2000 in a 6-well plate in 1 mL of antibiotic-free Opti-MEM (Gibco, #31985-062) medium plus 2 mL antibiotic-free cell-culture medium ...
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bioRxiv - Plant Biology 2024Quote: ... One microgram of total RNA was reverse transcribed using an oligo(dT)20 primer (Macrogen, South Korea) and M-MLV Reverse Transcriptase™ (Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... M and L with three independent plants for each of the 1205A and 1205B lines using the TRIzol kit (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: For each biological replicate a quarter of kidney tissue was used and dounced with a Wheaton Dounce tissue grinder in urea buffer (8 M Urea, 50 mM ammonium bicarbonate) supplemented with Halt protease-phosphatase-inhibitor cocktail (Thermo Scientific™). After clearing of the sample (16.000 xg ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... The capture-oligonucleotides were 3′ end labelled with a biotin-triethylene-glycol (biotin-TEG) spacer arm to allow capture using M-270 Streptavidin Dynabeads (Invitrogen, Waltham, USA). Capture-sequences for T ...
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bioRxiv - Cell Biology 2023Quote: ... each α-arrestin-containing lysate was incubated with GFP-nanobody-conjugated to Dynabeads M-270 Epoxy magnetic beads (#14301, Thermo Fisher Scientific). The supernatant was separated from the beads using a magnetic rack ...
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bioRxiv - Pathology 2023Quote: ... 3µm, 100Å) trap, and an Acclaim PepMap RSLC 50µm x 15cm, nanoViper (C18, 2µm, 100Å) separating column (ThermoFisher Scientific, Waltham, MA, USA) for mouse degradome samples or an Acclaim PepMap RSLC 75µm x 50cm ...
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bioRxiv - Plant Biology 2023Quote: ... according to the manufacturer’s protocol including the options to add 20 μl of 2 M dithiothreitol (Life Technologies/Thermo Scientific; https://www.thermofisher.com) per 1 ml buffer RLC and on-column Dnase digestion with Dnase I (Qiagen) ...
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bioRxiv - Physiology 2023Quote: ... 0.5-1 µg of total RNA was isolated from human islets and reverse transcribed using an M-MLV RT kit (Invitrogen, MA, USA). TaqMan primers (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... nano-capillary column packed with 1.9 μm C18-AQ resin (Dr. Maisch, Germany) mated to a metal emitter in-line with an Orbitrap Fusion Lumos (Thermo Scientific, USA). The column temperature was 45
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled with 10 μM EdU for 30 min before harvesting and processed using the Click-iT® EdU flow cytometry assay kit (C10643) (Thermo Fisher). In brief ...
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bioRxiv - Cell Biology 2023Quote: ... Collected proteins were incubated overnight with 100 μl of streptavidin-coupled Dynabeads (Dynabeads M-280 Streptavidin, Thermo Fisher Scientific, Waltham, MA, USA) at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... We used 2 µg of high-quality samples with A260/A230>2.0 and A260/A280>1.8 as determined with a ND-2000 spectrophotometer (Nanodrop Technologies, San Francisco CA, USA) as template for reverse transcription with the M-MLV Reverse Transcriptase kit (Thermo Fisher Scientific). Quantitative real-time PCR reactions were performed using Maxima SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... nano-capillary column packed with 1.9 μm C18-AQ resin (Dr. Maisch, Germany) mated to a metal emitter in-line with an Orbitrap Fusion Lumos (Thermo Scientific, USA). The column temperature was 45