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Citations for Thermo Fisher :
5601 - 5650 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: Coverslips were incubated for one hour at 37°C in 1mL of of 1:30 solution of Collagen I Rat Protein (Thermo Fisher Scientific) to 1X PBS ...
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bioRxiv - Neuroscience 2021Quote: The levels of RTN3 mRNA were validated by qRT-PCR using a pre-validated primer sequence (rat RTN3, Rn01498010_m1; cat no. 4351372; ThermoFisher Scientific, Loughborough, UK). Levels of protrudin mRNA were analysed using pre-validated primer sequences (rat Protrudin ...
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Neuroscience 2020Quote: ... regions were amplified from rat BDNF BAC construct (Koppel et al., 2018) using Phusion Hot Start II DNA Polymerase (Thermo Fisher Scientific) and cloned into pGL4.15 vector (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... the CeA of the rats were injected with 200 nl of retrograde tracer cholera toxin B Alexa Fluor 488 conjugate (CTB, Life Technologies, C34775). All surgical instruments were sterilized before surgery ...
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bioRxiv - Plant Biology 2021Quote: ... Roots were incubating with the primary antibody diluted at 1/100 (anti-PI4Kα1) and with secondary antibody diluted at 1/300 (anti Rat IgG, alexa Fluor 488 conjugate, InVitrogen-Molecular Probes, #A-21210) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were washed three times in PBS and stained with secondary antibodies (Goat anti-Rabbit Alexa Fluor 488, Goat anti-Rat Alexa Fluor 647, and/or Goat anti-Mouse Alexa Fluor 647; Invitrogen, 1:1000) and DAPI (1:10,000 ...
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bioRxiv - Neuroscience 2020Quote: ... we incubated the tissues overnight at room temperature in primary antiserum (rat monoclonal anti-mCherry 1:30000; Thermo Fisher Scientific, Cat.#M11217). Next ...
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bioRxiv - Bioengineering 2021Quote: ... Collagen-coated surfaces were used as a native protein control and were prepared by adding 0.15 mL/cm2 of 50 μg/mL type 1 rat-tail collagen (Thermo Fisher Scientific™) in 0.02 N acetic acid (Thermo Fisher Scientific™) ...
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bioRxiv - Physiology 2021Quote: ... Membrane damage was assessed using anti-IgG staining using an anti-rat antibody (IgG H+L AlexaFluor® 647 1:100, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed using TaqMan gene expression assays (Human-Assay ID Hs00917510_m1; and (Rat-Assay ID Rn01489483_m1; Applied Biosystems, Life Technologies, Carlsbad, CA). Gene expression was quantified by the comparative Ct value ...
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bioRxiv - Bioengineering 2022Quote: ... cluster of differentiation 31 (CD31, rat IgG2a) primary antibody and alpha-smooth muscle actin (α-SMA, rabbit IgG) primary antibody were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Physiology 2022Quote: 60 x 106 of mouse splenocytes were incubated for 15 min at 4°C in PBS containing 5% Normal Rat Serum (Thermo Scientific, 10710C) and anti-CD16/32 (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for one hour with the chicken anti-rat AlexaFluor 647 secondary antibody (1:400, #A-21472, RRID: AB_2535875, Thermo Fisher Scientific, USA). For PV staining ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated in 500 μL of primary antibody solution (rat anti-alpha Tubulin YL1/2 (MA1-80017, Invitrogen, CA, SAD), diluted 1:500 ...
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bioRxiv - Bioengineering 2022Quote: We prepared PFA-fixed lower brainstem tissue samples from 12-week-old WKY rats 24 h after introducing fluorescently labeled hydrogel conjugated with Alexa Fluor 594 C5 maleimide (A10256, Thermo Fisher Scientific) to their RVLM as described above ...
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were dissociated from dissected embryonic day 18 rat hippocampi as previously described and cultured in Neurobasal with B27 supplement (Thermo Fisher Scientific) (Thomas et al. ...
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bioRxiv - Microbiology 2020Quote: ... followed by three 5 min washes in PBS-T and a subsequent incubation with species-specific secondary antibody (goat-anti-rabbit/rat/mouse IgG-HRP, Invitrogen 1:2500) for 1 h in 5 % milk in PBS-T ...
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bioRxiv - Neuroscience 2019Quote: ... cat# 703-505-155; donkey anti-rat Cy3, 1:400, Invitrogen, A-21209; donkey anti-mouse Cy5, 1:400, Invitrogen, A-31357) in PBS at 4 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... slides were incubated in a wet chamber with the secondary antibodies (Alexa Fluor Goat anti-Rat 594, Invitrogen, 1:1000 and Alexa Fluor Goat anti-Guinea Pig, Invitrogen, 1:1000) in PBS/Triton/BSA for 1 h at room temperature in the dark ...
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bioRxiv - Immunology 2020Quote: ... and then incubated with secondary antibodies for 30 min at room temperature (AF555-goat anti-rat IgG (Invitrogen, Carlsbad CA, 1:500); AF647-sAv (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with secondary antibody Alexa Fluor 647-or Alexa Fluor 488-conjugated goat anti-rat secondary antibody in above staining buffer for 1 h at RT (1:500, both Thermo Fisher Scientific). Finally ...
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bioRxiv - Immunology 2020Quote: ... Peyer’s patches were then stained with Alexa Fluor 488-conjugated anti-rat IgG Ab and Alexa Fluor 647-conjugated phalloidin to detect F-actin (both Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: The concentration of secreted Tgfβ1 ligand levels was measured by taking 3-day conditioned media at d5 of culture with Tgf beta-1/LAP Rat Uncoated ELISA Kit with Plates by following the manufacturer’s protocol (ThermoFisher Scientific, Waltham, MA). The cells were cultured in a 2% FBS media for ELISA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the sections were incubated for 1 h in 1:500 dilution of goat anti-rat Alexa 488 secondary antibody (ThermoFisher, Loughborough, UK) followed by several rinses with dH2O ...
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bioRxiv - Immunology 2022Quote: ... They were both determined to be of the IgG2a subclass by using the Pro-DetectTM Rapid Antibody Isotyping Kit-Rat (Thermo Fisher Scientific).
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bioRxiv - Pathology 2020Quote: ... total ribonucleic acid was isolated from cultured cells or rat carotid arteries (pulverized in liquid nitrogen) by using a Trizol reagent (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: REF52 cells (an immortal line of post-crisis Fischer rat embryo cells were grown in Minimum Essential Medium α (MEM α) (Gibco/Invitrogen) supplemented with 10% bovine growth medium (BGS ...
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bioRxiv - Synthetic Biology 2019Quote: ... and the supernatant was taken to measure the amount of secreted IL-10 using the IL-10 Rat ELISA Kit (Invitrogen, catalog # BMS629). The resulting values were normalized based on the cell viability ...
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bioRxiv - Physiology 2020Quote: ... Sections were then incubated with Alexa Fluor® 488 conjugated goat anti-rat immunoglobulin G (IgG; [H+L]; 1:100, Life Technologies) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... LPS-stimulated BMDCs (2.5×105 cells per well) were seeded into 3D collagen (type I, rat tail)/Matrigel matrix (both from Corning, via Thermo Fisher Scientific) supplemented with 10% minimum essential medium alpha medium (MEMalpha ...
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bioRxiv - Neuroscience 2019Quote: ... The coverslips were then washed 3 times with PBS and incubated with the secondary antibody Alexa Fluor donkey anti-rat 488 (Life Technologies, A21208), Alexa Fluor donkey anti-rabbit 555 (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 1-2 times with PBS and incubated for 2 hours in Alexa Fluor conjugated goat anti-rabbit/ rat/ chicken secondary antibodies (Thermo Fisher Scientific), secondary antibodies washed in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were fixed with 4% paraformaldehyde for 10 min before addition of anti-rat Alexa Fluor 555 and anti-mouse Alexa Fluor 488 secondary antibodies (Thermo Fisher Scientific). Fluorescently-labelled DNA fibers were imaged using a Nikon Eclipse Ni microscope with NIS-Elements software (Nikon Instruments) ...
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bioRxiv - Immunology 2021Quote: ... Unlabeled primary antibodies were detected using secondary antibodies (goat anti-rat Alexa Fluor 488 or goat anti-rabbit Alexa Fluor 647; both from Thermo Fisher Scientific). Tissue was counterstained with DAPI (10pg/ml final dilution in PBS ...
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bioRxiv - Pathology 2021Quote: ... the sections were incubated with goat anti-rat secondary antibody conjugated to Alexa Fluor 594 for 1 hour (diluted 1:500, Thermo Fisher Scientific). Proliferating cell nuclear antigen (PCNA ...
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bioRxiv - Neuroscience 2021Quote: Rats (N = 4) were infused unilaterally with AlexaFluor-594 conjugated cholera toxin B (CTb; 5 μg/μL; Life Technologies, San Diego, CA) into the mOFC (AP +4.1 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then centrifuged and washed in FACS Buffer and incubated on ice (15 min) with secondary antibody donkey anti-rat IgG Alexa Fluor-488 (Thermo Fisher Scientific). Cells were centrifuged and washed in FACS Buffer and flow cytometry data was collected using the FACS Calibur (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... fixed cells were rinsed in PBS and incubated 1 hour at RT 10% blocking solution in PBS with a mixture of Alexa Fluor 488 conjugated goat anti-rat IgG antibody (diluted 1:300, Cat. A-11006, Molecular Probes Inc.) and Alexa Fluor 555 conjugated goat anti-rabbit IgG antibody (diluted 1:300 ...
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bioRxiv - Cell Biology 2021Quote: ... transfected cells were seeded at low density 1000 cells/well in 96-well microtiter IncuCyte® ImageLock Plate (Essen BioScience) pre-coated with rat tail Collagen type I (Gibco™). After 24Lhours ...
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded onto a 96-well microtiter IncuCyte® ImageLock Plate (Essen BioScience) pre-coated with rat tail Collagen type I (Gibco™). When cells were at full confluence ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed three times in washing solution for 5 min at RT and incubated with secondary antibodies (donkey anti-rat Alexa Fluor 488 (1:200, A-21208, Thermo Fisher Scientific) and donkey anti-mouse Alexa Fluor 555 (1:200 ...
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bioRxiv - Immunology 2021Quote: ... anti-CD326/EpCAM [G8.8] (PE-Cy7) anti-CD107a [eBio1D4B] (PE) and Rat IgG2a kappa Isotype control [eBR2a] (PE) were purchased from Thermo Fisher (Waltham, Massachusetts). Anti-KLRG1 [MAFA] (APC) ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... and unlabeled anti-LTβR mAb and subsequently counterstained with streptavidin-Alexa 488 and goat-anti-rat-Alexa 488 (all Invitrogen, Breda, The Netherlands), respectively ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... all samples were repeatedly washed in 1x PBS with 0.2% saponin and incubated with secondary antibodies donkey anti-rat Alexa fluor 488 (Thermo Fisher Scientific, A21208) or donkey anti-rabbit Alexa fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... at 4°C and bound antibodies were detected by fluorescently labelled anti-rat AlexaFluor 488 and anti-rabbit IgG AlexaFluor 647 antibodies (Thermo Fisher Scientific). Nuclei were counterstained with DAPI (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then stained with the following antibodies: rat CD31-eFluor450 (1/500; eBiosciences™, Thermo-Fisher Scientific, San Diego, CA, USA), rat CD45-eFluor450 (1/500 ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were washed with 0.3% Triton X-100 in PBS for three times and the secondary goat anti-rat-Alexa Fluor 568 antibody (Life Technologies, A11077) was added to the slide for 1 h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... were incubated with fluorescent secondary antibodies (Goat anti-mouse IgG DyLight 800 ThermoFisher #SA5-10176 and Goat anti-rat IgG DyLight 800 ThermoFisher #SA5-10024), and images acquired on a LI-COR Odyssey instrument ...
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bioRxiv - Bioengineering 2022Quote: ... The rat tail collagen I protein and GFOGER peptide peptide were subsequently blocked using methyl-PEG24-amine (MA-PEG24) (Thermo Fisher Scientific). Post functionalization ...