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Citations for Thermo Fisher :
5551 - 5600 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... nano-capillary column packed with 1.9 μm C18-AQ resin (Dr. Maisch, Germany) mated to a metal emitter in-line with an Orbitrap Fusion Lumos (Thermo Scientific, USA). The column temperature was 45
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bioRxiv - Cell Biology 2023Quote: ... magnetic beads bound to antibody recognizing the FLAG epitope tag were prepared in-house by coupling Dynabeads M-70 Epoxy (Thermo Fisher Scientific) to FLAG M2 antibody (Millipore Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... samples were stained for 90 min with 1.65 x 10−7 M Alexa Fluor 488 phalloidin (Invitrogen, Life Technologies Corporation, Oregon, USA) in 1x DPBS ...
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bioRxiv - Cell Biology 2023Quote: ... Collected proteins were incubated overnight with 100 μl of streptavidin-coupled Dynabeads (Dynabeads M-280 Streptavidin, Thermo Fisher Scientific, Waltham, MA, USA) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ApoE was immunoprecipitated from matched participant samples of 20 μL CSF and 2 μL plasma via a monoclonal antibody to human apoE (HJ15.3) (provided by the Holtzman Lab) conjugated to M-270 Epoxy Dynabeads (Life Technologies/Invitrogen, Carlsbad, CA). The CSF samples were prepared by combining 20 μL of neat CSF with 100 μL of the apoE3*/apoE4* astrocyte media internal standard ...
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bioRxiv - Cancer Biology 2023Quote: ... and washed for 3min on a C18 reverse phase precolumn (3μm particle size, 100 Å pore size, 75 m inner diameter, 2 cm length, from Thermo Fisher Scientific). Peptides were separated on an Aurora C18 reverse phase resin (1.6μm particle size ...
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bioRxiv - Cell Biology 2022Quote: For the analysis of metabolic pool sizes the eluting compounds were detected in negative ion mode using full scan measurements in the mass range m/z 77 – 770 on a Q-Exactive HF high resolution MS (Thermo Fisher Scientific). The heated electrospray ionization (HESI ...
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bioRxiv - Evolutionary Biology 2023Quote: ... into the packaging 293T cells (with molar ratio for Luc-T20: N: M/E: S as 3: 1: 1: 1) with LipofectamineTM 2000 (Thermo Fisher Scientific). At 24 and 48 hours post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated in 0.1 M citrate buffer in a 70°C water bath for 30 min and then blocked with CAS block (Thermo Fisher, USA). The slides were washed and then incubated with a TUNEL reaction mixture for 60 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Mono-biotinylated Fab’ fragments of goat anti-mouse Ig(G+M) were labeled with AF546 using an Alexa Fluor 546 antibody labeling kit (Thermo Fisher Scientific). The molar ratio of AF546 to Fab’ in the AF546-labeled Fab’ was ∼2 determined by a Nanodrop spectrophotometer (Nanodrop Technologies).
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bioRxiv - Physiology 2023Quote: ... 0.5-1 µg of total RNA was isolated from human islets and reverse transcribed using an M-MLV RT kit (Invitrogen, MA, USA). TaqMan primers (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... nano-capillary column packed with 1.9 μm C18-AQ resin (Dr. Maisch, Germany) mated to a metal emitter in-line with an Orbitrap Fusion Lumos (Thermo Scientific, USA). The column temperature was 45
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bioRxiv - Plant Biology 2023Quote: ... We used 2 µg of high-quality samples with A260/A230>2.0 and A260/A280>1.8 as determined with a ND-2000 spectrophotometer (Nanodrop Technologies, San Francisco CA, USA) as template for reverse transcription with the M-MLV Reverse Transcriptase kit (Thermo Fisher Scientific). Quantitative real-time PCR reactions were performed using Maxima SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: 6µm-thick sections from tissue blocks of the above-mentioned regions were cut and mounted on superfrost+ glass slides (Thermo Scientific, USA). All sections were stained for synaptophysin (C-terminal) ...
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bioRxiv - Physiology 2023Quote: For the analysis of metabolic pool sizes the eluting compounds were detected in negative ion mode using full scan measurements in the mass range m/z 50–750 on a Q-Exactive HF high resolution MS (Thermo Fisher Scientific). The heated electrospray ionization (ESI ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoaffinity magnetic beads were prepared by coating antibodies onto Dynabeads® M-270 Epoxy beads using the Dynabeads Antibody Coupling Kit (14311D, ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The capture-oligonucleotides were 3′ end labelled with a biotin-triethylene-glycol (biotin-TEG) spacer arm to allow capture using M-270 Streptavidin Dynabeads (Invitrogen, Waltham, USA). Capture-sequences for T ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA synthesis was performed on 1 µg of total RNA with the RevertAid H Minus Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific) using random primers p(dN)6 (Roche) ...
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bioRxiv - Microbiology 2023Quote: Grids were imaged with a Talos L120C TEM operated at 120 keV and equipped with a LaB6 filament and a Ceta-M camera with a 4k × 4k CMOS (Thermo Fisher Scientific). Electron micrographs were recorded at magnifications of 5,300 × ...
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bioRxiv - Cell Biology 2023Quote: ... 50% sucrose and 0.0015% hydrogen peroxide) [41] with the following minor modification: 150ul of Dynabeads M-270 streptavidin (Invitrogen, catalog no. 65306) was used to purify the biotinylated DNA ...
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bioRxiv - Cell Biology 2023Quote: ... the compounds were eluted and detected in negative ion mode using the full scan measurements in the mass range m/z 50 – 750 on a Q-Exactive HF high resolution MS (Thermo Fisher Scientific). The heated electrospray ionization (ESI ...
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bioRxiv - Cell Biology 2023Quote: ... connected to an analytical column (EASY-Spray column, 50 m × 75 μm ID, PepMap RSLC C18, 2 μm, 100 Å, Thermo Scientific) using the autosampler of an Easy nLC 1000 (Thermo Scientific ...
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bioRxiv - Physiology 2023Quote: ... 5 µm sections were stained for Ki-67 and TTF-1 (nuclear thyroid marker) using anti-Mo/Rt Ki-67 (Invitrogen, Waltham, MA) and anti-TTF-1 (D2E8 ...
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bioRxiv - Synthetic Biology 2023Quote: Phage particles were blocked with phosphate-buffered saline (PBS) with 5% BSA and depleted for nonspecific binders on M-280 streptavidin coated magnetic beads (Thermo Fisher Scientific). Biotinylated Human CD40L (Acro CDL-H52Db ...
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bioRxiv - Neuroscience 2024Quote: ... Complementary DNA was synthesized using 35 to 200 ng of purified RNA using oligo-dT primers and M-MLV Reverse Transcriptase (Invitrogen, 28025-013). Real-time qPCR was performed using SYBRTM Green PCR Master Mix (Applied Biosciences ...
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bioRxiv - Biophysics 2024Quote: ... with 5 µM protein sprayed from 400 mM AmAc on a Q Exactive Ultra-High Mass Range (UHMR) orbitrap mass spectrometer (Thermo Fisher Scientific). The instrument was modified to allow for surface induced dissociation ...
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bioRxiv - Biophysics 2024Quote: ... Trp concentration was determined by UV absorbance at 280 nm with the extinction coefficient ε =5540 M-1 cm-1 using a Nanodrop 2000c spectrophotometer (Thermo Scientific) in droplet mode.
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bioRxiv - Cancer Biology 2024Quote: ... De-crosslinking was conducted using 4 µl of 5 M NaCl and 1 µl of 20 mg/ml proteinase K (Thermo Fisher Scientific) for 4 h at 60 °C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... in 0.1 M PB for 10 minutes and developed with a streptavidin-solution (1:500 in 0.5% Tx-100 in 0.1 M PB, Life Technologies, Carlsbad, CA, USA). The tissue was washed three times in 0.1 M PB and once in 0.025 M PB ...
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bioRxiv - Cell Biology 2024Quote: Cells and liver tissues were lysed in M Cellytic Lysis Buffer containing 1% protease inhibitor (Halt™ Protease Inhibitor Cocktail; ThermoFisher Scientific) for 15 min using a cell disruptor or homogenizer ...
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bioRxiv - Immunology 2024Quote: Liver was scratched using a 100 µM cell strainer (Falcon 352360) and a syringe plunge in PBS (Eurobio CS1PBS01-01) supplemented with EDTA 2 mM (Invitrogen 15575-038) and BSA 0.5% (Sigma-Aldrich A7906 ...
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bioRxiv - Molecular Biology 2024Quote: The MS2 spectra were screened for sulfated HexNAc oxonium marker ions (HexNAc1Sulfo1 [M+H]+/ 284.0435 and Hex1HexNAc1Sulfo1 [M+H]+/ 446.0963) using a scan filter in Thermo Xcalibur Qual Browser software (Version 2.2, Thermo Fisher Scientific, Bremen, Germany) as described by Zuniga-Banuelos et al ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... 0.8 µM aprotinin, 50 µM bestatin, 15 µM E-64, 5 mM EDTA, 20 µM leupeptin, 10 µM pepstatin A; Thermo Fisher 78429). At 4°C ...
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bioRxiv - Genomics 2024Quote: ... 50% sucrose and 0.0015% hydrogen peroxide) (Zhang et al., 2020) with the following minor modification: 150ul of Dynabeads M-270 streptavidin (Invitrogen, catalog no. 65306) was used to purify the biotinylated DNA ...
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bioRxiv - Biophysics 2021Quote: ... Cultured cells were stained with mAb 0614 or human chimera MAb 0614-5 as the primary antibody and Alexa Fluor 488– or 647–conjugated anti-rat or human-Ig polyclonal antibody (Thermo Fisher Scientific) as the secondary antibody ...
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... and secondary antibodies Alexa Fluor 488-conjugated goat anti-rabbit IgG and Alexa Fluor 633-conjugated goat anti-rat IgG were from Invitrogen (Carlsbad, CA). Vibrant DiD membrane-labeling reagent was from Thermo Fisher (Waltham ...
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bioRxiv - Cell Biology 2020Quote: For live-cell imaging of neurons, primary cultures of rat cortical neurons were prepared as described (Govind et al., 2012) using Neurobasal Medium (Thermo Fisher; 21103049), 2% (v/v ...
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bioRxiv - Genetics 2021Quote: ... in PBS-T and subsequently incubated for one hour with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG secondary antibody at 1:20,000 (Invitrogen cat. number 31470). Blots were then rinsed again once quickly ...
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bioRxiv - Genetics 2021Quote: ... The sections were rinsed in PBS/Tween buffer before incubating for 60 min in 1:200 goat anti-rat IgG (H+L) antibody conjugated to horseradish peroxide (HRP) (Invitrogen, ThermoFisher Scientific). The slides were then developed in 3,3’ diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were incubated with the following primary antibodies in blocking buffer overnight at 4 °C: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific) and mouse anti-OCT4 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies include: goat-anti-rat polyclonal Alexa Fluor and goat-anti-mouse polyclonal Alexa Fluor 488 (both 1:1000, Thermofisher, Waltham, MA).
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bioRxiv - Neuroscience 2022Quote: ... counted and re-seeded on poly-D-Lysine coated dishes containing Rat astrocytes in neuronal medium (B27 Electrophysiology medium (Gibco, A14137-01) supplemented with 1× MEM non-essential amino acids ...
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bioRxiv - Molecular Biology 2020Quote: ... the slides were incubated with the following secondary antibodies (anti-rat IgG Alexa Fluor 594, Molecular Probes A-11007; anti-mouse IgG1 Alexa Fluor 488, Molecular Probes A-21121 ...
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bioRxiv - Molecular Biology 2020Quote: ... Alexa Fluor 488-conjugated goat anti-rat IgG (H + L) antibodies and Alexa Fluor 594-conjugated goat anti-mouse IgG (H + L) antibodies (ThermoFisher Scientific, A11005) were used as secondary antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... and then the corresponding secondary antibodies: anti-rat IgG (Alexa Fluor 488 conjugated, Molecular Probes A21210 and Dylight 550 Invitrogen SA5-10027) and IgM (Dylight 488 conjugated ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides were then washed with PBS and incubated with Alexa Fluor 488-conjugated chicken anti-rat antibody at 1:200 dilutions (Life technologies, A21470). Slides were washed wit PBS and incubated for 45 minutes at room temperature with mouse anti-BrdU (Becton Dickinson ...
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bioRxiv - Bioengineering 2019Quote: ... Secondary antibodies at 1:200 dilution were added for 90 min at room temperature (goat anti-rat with AlexaFluor 647 ab150159, Abcam, Cambridge, UK; goat anti-rabbit with AlexaFluor 488 A11006, Invitrogen, Carlsbad, CA). DAPI at 2 µg/mL was added for 10 min at room temperature to counterstain nuclei ...
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Tracking of quiescence in Leishmania by quantifying the expression of GFP in the ribosomal DNA locusbioRxiv - Microbiology 2019Quote: ... Samples were subsequently incubated for 1.5 hrs with Alexa 555 goat polyclonal anti rat IgG (1/1000) and Alexa 488 goat polyclonal anti rabbit IgG (1/1000) (Thermo fisher scientific). After two washes with PBS the samples were mounted with Prolong Gold antifade (Thermo fisher scientific ...
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bioRxiv - Immunology 2019Quote: ... lung tissues were incubated with primary antibodies diluted in PBS with 0.3% TritonX-100 on a rocker at 4° C overnight. Primary antibodies used (Fig. 5) were: rat anti-E-cadherin (E-Cad, 131900, Invitrogen; 1:1000), goat anti-CCSP (1:2,500 ...