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Citations for Thermo Fisher :
5751 - 5800 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... equipped with two Waters pre-columns and a Waters nano m/z analytical column (75 μm × 250 mm, 3 μm, 100 Å, Thermo Fisher Scientific) (Gronemeyer et al. ...
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bioRxiv - Neuroscience 2023Quote: ... An Acclaim PepMap (75μm x 2 cm) trap column together with a EasySpray ES803 column (75 µm x 50 mm, 100 Ȧ) (both Thermo Fisher Scientific) were employed for nanoLC with 3 µL peptide extract being injected.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 μg total RNA was then used to synthesize cDNA using a M-MLV Reverse Transcriptase Enzyme System (Life Technologies, Thermo Fisher Scientific) and OligoT primers ...
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bioRxiv - Neuroscience 2022Quote: ... One microgram of total DNase-treated RNA was reverse-transcribed in a 20-μl reaction volume using M-MLV (Thermo Fisher Scientific, USA). The resulting cDNA was diluted five-fold and RT-qPCR was performed with Eva™ Green PCR Master Mix (Biotium ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed 3x with 0.1 M PB and incubated in 0.1 M PB containing 10% goat serum and conjugated secondary antibody (Alexa Fluor 647 anti-mouse, dilution 1:500, Invitrogen cat #A-21235) for 1 h at room temperature.
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bioRxiv - Neuroscience 2024Quote: ... excised temporal bones were post fixed in 4% paraformaldehyde for 20 minutes on ice and decalcified in 0.1 M ethylenediaminetetraacetic acid (EDTA, Fisher Scientific, cat. # AC327205-000) overnight for ∼16 hours ...
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bioRxiv - Neuroscience 2023Quote: ... into 18 µ m-thick sections and collected on pre-cleaned Superfrost Plus Microscope Slides (Fisherbrand, Fisher Scientific, Cat. No. 12-550-15). Microscope slides were kept at -20°C until use ...
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bioRxiv - Neuroscience 2022Quote: Wild-type mice received a single iontophoretic injection of each anterograde tracer: biotinylated dextran amine (BDA; 10 kDa; 5% solution in 0.125 M phosphate buffer, pH 7.4; Thermo Fisher Scientific, Cat. No. D1956) or Phaseolus vulgaris leucoagglutinin (PHA-L ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed to detect the presence of influenza A virus (M gene) using AgPath-ID™ One-Step RT-PCR Kit (Thermo Fisher Scientific). Positive influenza samples were then selected for whole genome sequencing using next generation sequencing (NGS ...
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bioRxiv - Microbiology 2023Quote: ... Total cDNA was obtained by reverse transcription using random hexamers pd(N)6 and M-MLV reverse transcriptase (Life Technologies, Carlsbad, CA, USA) at 42 °C for 50min ...
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bioRxiv - Biochemistry 2023Quote: The RBD protein was incubated for 30 min at 21 °C and 750 rpm with a 10 molar equivalent of sulfo-m-maleimidobenzoyl-N-hydoxysuccinimide ester (Sulfo-MBS) (Thermo Scientific; MW 416.30). The excess crosslinker was removed by passing the protein through a PD-10 column using a PBS ...
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bioRxiv - Cell Biology 2023Quote: Sections (12 μm) were cut and allowed to dry at room temperature for 1 h on microscope slides (SuperFrostPlus; Menzel-Glaser; Thermo Fisher, Victoria, Australia). Sections were next incubated with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The combined lower layer was then washed with 300 μl of 1 M KCl followed by 300 μl of water and vacuum dried completely (Savant SpeedVac SPD130DLX vacuum concentrator, Thermo Fisher Scientific, USA) to obtain the dried lipid extract.
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bioRxiv - Microbiology 2023Quote: ... and 0.6 mL of ice cold isopropanol was added along with 1/10th volume sodium acetate solution (Invitrogen, 3 M, pH 5.2; R1181) and 1 μL GlycoBlue coprecipitant (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: A Thermo Trace 1310 Gas Chromatograph with a Thermo Scientific TraceGOLD TG-5SILMS column (30 m long, 0.25 mm inner diameter, 0.25 μm film thickness) (Thermo Fisher Scientific, Waltham, MA, USA) was used to separate the geraniol in the samples ...
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bioRxiv - Biochemistry 2023Quote: ApoE was immunoprecipitated from matched participant samples of 20 μL CSF and 2 μL plasma via a monoclonal antibody to human apoE (HJ15.3) (provided by the Holtzman Lab) conjugated to M-270 Epoxy Dynabeads (Life Technologies/Invitrogen, Carlsbad, CA). The CSF samples were prepared by combining 20 μL of neat CSF with 100 μL of the apoE3*/apoE4* astrocyte media internal standard ...
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bioRxiv - Neuroscience 2023Quote: ... Frozen cortical tissue was homogenized with a micro pestle and electric rotor in 200 μL of mammalian protein extraction reagent (M-PER) (Thermo Scientific, Cat. #: 78501) containing ProBlock Gold Extra Strength Protease Inhibitor Cocktail Kit (GoldBio ...
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bioRxiv - Physiology 2023Quote: Confluent HUVECs were stimulated by 50 ng/ml human VEGF-A for indicated lengths of time and lysed in M-PER buffer (Thermo Fisher Scientific, USA) supplemented with protease and phosphatase inhibitors (Mini Complete/PhosphoStop ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μL ribodepleted RNA was mixed with 4 μL 5× TGIRT buffer (2.25 M NaCl, 25 mM MgCl2, 100 mM Tris-HCl, pH 7.5; Ambion RNase-free Buffer Kit AM9010), and 1 μL random hexamer primers (xGen) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Coronal brain slices were obtained using vibratome sectioning (80 μm), and immunostained with antibodies against GFP: rabbit anti-GFP (A-6455, 1:1,000) and goat antirabbit AF488 (Invitrogen, A-11008, 1:1,000). The sections were incubated in DAPI and mounted In Mowiol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific, cat# 28025013). For quantitative Real Time Polymerase Chain Reaction (RT-qPCR) ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were initially loaded onto a trap column (Acclaim C18 PepMap nano Trap × 2 cm, 100 μm I.D, 5-μ m particle size and 300-Å pore size; Thermo Fisher Scientific) at 15 μl min−1 for 3 min and subsequently switched to the analytical column (Acclaim RSLC C18 PepMap Acclaim RSLC nanocolumn 75 μm × 50 cm ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting supernatant containing soluble CCTX2 was then filtered through a 0.22 μm PES membrane filter and loaded onto a 5 mL Tricorn column packed with immobilized D-galactose gel (Pierce™, Thermo Scientific™) previously equilibrated with 6 CV loading buffer (20 mM Na-HEPES ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 μl forward primers (5 μM) and 0.2 μl reverse primers (5 μM) in 96-well plates (MicroAmp Fast 96-well Reaction Plate, Applied Biosystems for Life Technologies). The reactions were performed in triplicates on a QuantStudio 5 Real-Time PCR System (Thermo Fischer Scientific ...
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bioRxiv - Physiology 2023Quote: HA isolation was performed by biomagnetic precipitation following protocols reported previously with minor modification.12 Dynabeads™ M-280 streptavidin-coated superparamagnetic beads (Ref. 11206D, Thermo Fisher Scientific) were first washed according to the manufacturer’s directions and then incubated (2 hours at room temperature under gentle agitation ...
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bioRxiv - Pathology 2023Quote: ... OCT embedded tissues were sectioned at 10 μm thickness and glycol methacrylate embedded at 3 μm and transferred to positively charged glass slides (Superfrost glass slides, Thermo Fisher, IL, USA). Hematoxylin/eosin (H&E ...
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bioRxiv - Developmental Biology 2024Quote: ... 2-DG was solubilized as a 1 M stock solution in DMEM medium (without glucose, pyruvate and glutamine, Gibco, cat. no. A14430-01) and used at 10 mM ...
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bioRxiv - Cell Biology 2024Quote: HEK293T or HeLa Kyoto cells expressing the indicated tagged proteins were washed twice with ice-cold PBS and lysed in M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, 78501) containing protease and phosphatase inhibitors ...
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bioRxiv - Immunology 2024Quote: ... The acid-extracted histones were collected in the supernatant fraction after centrifugation at 650g for 10 min at 4 °C and were neutralized with 2 M NaOH at 1/10 the volume of the supernatant before quantifying protein concentration using BCA® Protein Assay kit (Thermo Scientific #23225)139 ...
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bioRxiv - Microbiology 2022Quote: ... α-mannose binding lectin C (primary: mAb 14D12, Abcam catalog no. ab106046; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α- IgM (mAb II/41 ...
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bioRxiv - Neuroscience 2021Quote: ... mAb24B10 (DSHB, 1:50), anti-OLLAS (Funakoshi, 1:200) and goat anti-rat IgG(H+L) Alexa Flour 488 conjugate (Life Technologies, 1:400).
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with PBS for 3 × 10 minutes prior to incubation overnight at 4°C with the Alexa secondary cocktail: Alexa Fluor 555 dye-conjugated goat anti-rat (1:1000, Invitrogen, Eugene, OR, USA) and Alexa Fluor 488 dye-conjugated goat anti-mouse (1:600 ...
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bioRxiv - Developmental Biology 2019Quote: ... for 1 hour at room temperature and Alexa647-conjugated goat-anti rabbit or goat anti-rat secondary antibodies highly cross-adsorbed against IgG from other species (ThermoFisher Scientific, 1:200) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... chips were washed with 100 ul PBS per channel and stained with the following secondary antibodies diluted 1: 100 in 5% BSA in PBS for 2 hours at room temperature: goat anti-rat Alexa Fluor 488 (Thermo Fisher Scientific A11006), donkey anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific A31573) ...
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bioRxiv - Developmental Biology 2021Quote: ... They were then incubated with secondary antibodies for 1 hour at room temperature [donkey Alexa 488 anti-rat (1:1000, Invitrogen, Carlsbad, CA, #A21208), goat Alexa 555 anti-rabbit (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... Roots were incubating with the primary antibody diluted at 1/100 (anti-PI4Kα1) and with secondary antibody diluted at 1/300 (anti Rat IgG, alexa Fluor 488 conjugate, InVitrogen-Molecular Probes, #A-21210) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed using TaqMan gene expression assays (Human-Assay ID Hs00917510_m1; and (Rat-Assay ID Rn01489483_m1; Applied Biosystems, Life Technologies, Carlsbad, CA). Gene expression was quantified by the comparative Ct value ...
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bioRxiv - Cell Biology 2021Quote: ... Primary neuronal cultures from cortices of 2-day-old rats were prepared and maintained at 37 °C with a 5% CO2-containing atmosphere in neurobasal medium (Life Technologies, 21103-049) supplemented with 2% B27 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... α-mannose binding lectin A (primary: mAb 8G6, Hycult catalog no. HM1035; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α-mannose binding lectin C (primary ...
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bioRxiv - Neuroscience 2021Quote: ... coverslips were flipped onto 60 mm dishes containing 15 DIV rat hippocampal glial cells cultured in Neurobasal plus medium (Gibco Thermo Fisher Scientific, #A3582901) supplemented with 2 mM glutamine and 1x B27TM plus Neuronal supplement (Gibco Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were incubated in primary antibody (chicken anti-GFP, Aves labs GFP-1010, 1/500; goat anti-PDGFRA, R&D Systems AF1062, 1/200; rat anti-SOX2, ThermoFisher 14-9811-82 ...
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bioRxiv - Neuroscience 2022Quote: ... rat neurons were seeded in eight-well Lab-Tek II chambered cover glasses (German #1.5 borosilicate glass; Thermo Fisher Scientific, cat. no. 155409) at a density of 100,000–110,000 neurons per well ...
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bioRxiv - Cancer Biology 2020Quote: ... After incubation with secondary antibodies (Alexa-Fluor 594 goat antirabbit, Invitrogen Cat # A-11012 and Alexa-Fluor 488 goat anti-rat, Invitrogen Cat # A-11006) diluted 1:1000 in blocking solution ...
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bioRxiv - Cell Biology 2019Quote: REF52 cells (an immortal line of post-crisis Fischer rat embryo cells were grown in Minimum Essential Medium α (MEM α) (Gibco/Invitrogen) supplemented with 10% bovine growth medium (BGS ...
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bioRxiv - Microbiology 2020Quote: ... 474-1506), anti-mouse at 1:3,000 (Insight Biotechnology, Cat no. 474-1506) or anti-rat at 1:4000 (Life technologies, Cat. no 629520). The blots were visualised using ECL Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The slides were washed three times for 10 min with 0.033% Triton X-100 and incubated with an anti-rat antibody conjugated with Alexa Fluor® 488 (1:1000 dilution, Thermo Fisher Scientific) in blocking buffer for 1 hr at room temperature ...
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bioRxiv - Immunology 2021Quote: ... for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088, Thermo Fisher Scientific; A18869, Thermo Fisher Scientific) for monoclonal antibodies ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated and unconjugated primary antibodies were detected by incubating with Streptavidin-PE (Pharmingen, 534061) and Rat anti-mouse IgG2a antibody (Invitrogen, 17-4210-80) respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa 488-conjugated donkey anti-goat and Alexa 488-conjugated donkey anti-rat antibodies were purchased from Life Technologies (Grand Island, NY, USA).
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bioRxiv - Physiology 2021Quote: ... and incubated with secondary antibody (conjugated goat anti-rat Alexa fluoro 546, 1:1,000 dilution, A11081; Invitrogen by ThermoFisher Scientific; Waltham, MA, USA) for 30 minutes ...