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Citations for Thermo Fisher :
5851 - 5900 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... of two sub-clusters in the viral population in infant 1580i and one env gene (M.2) that represents one major cluster viruses in mother 1580m were codon optimized and chemically synthesized (Thermo Fisher, Waltham, MA, USA). The env genes were cloned into pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA was synthesized by RT-PCR from 1 μg of purified RNA with the M-MLV reverse transcriptase (Thermo Fisher Cat#: 28025-013) and random primers (Thermo Fisher Cat# ...
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bioRxiv - Cancer Biology 2022Quote: Cells treated with 1 µM RSL3 for 4h were stained with 200 µM C11 581/591 BODIPY™ or Annexin V Pacific Blue™ conjugate (Molecular Probes®) as per manufacturer protocol ...
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bioRxiv - Neuroscience 2019Quote: ... mice were decapitated and their brain tissue was first embedded in cryomolds (Sakura Finetek, Cat. No. 25608-924) filled with M-1 Embedding Matrix (Thermo Scientific, Cat. No. 1310) then quickly fresh-frozen on dry ice ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL of SAT solution (used to dilute alcian blue solution) containing 30 μL of 18 M sulfuric acid (Acros Organics, 7664-93-9), 75 μL of triton x-100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... and adherent cells were subjected to LIVE/DEAD viability staining using 5 µM of Syto9 and 30 µM of propidium iodide (PI) according to the manufacturer’s instructions (LIVE/DEAD® BacLight™, Molecular Probes, Life Technologies). Fluorescence images were captured with Zeiss LSM510 META confocal microscope using Zeiss LSM 3.2 software (Zeiss GmbH ...
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bioRxiv - Physiology 2020Quote: ... extractions and then precipitated using 1/10 volume of 3 M sodium acetate and 2.5 volumes of 100% ethanol (HoneywellTM, Fisher Scientific UK Ltd, Loughborough, UK) at −80°C ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysates were incubated for 2 h at 4°C with IgG-coupled Dynabeads (Dynabeads M-270 Epoxy; Invitrogen, Thermo Fisher Scientific, Basel, Switzerland). The beads were washes 5 times with lysis buffer and proteins were eluted in TEV buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM EDTA pH 8) agarose gel for 75 m at 80V using GeneRuler 100 bp plus DNA ladder (Thermofisher Scientific, Waltham, MA, USA).
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bioRxiv - Microbiology 2022Quote: ... Negative and positive mode data were collected at a mass range of 70–1050 m/z in centroid data mode on a Thermo QExactive (Thermo Fisher Scientific, Waltham, MA). Fragmentation spectra (MS/MS ...
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bioRxiv - Neuroscience 2022Quote: ... 40-μm frozen coronal sections were cut through the rostrocaudal extent of the cortical mantle (Microm HM 430 freezing microtome, Thermo Fisher Scientific, Waltham, MA). Sections were collected in a cryoprotectant solution (30% ethylene glycol/15% sucrose/0.05% sodium azide in TBS ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration of the supernatant was determined using A280 nm with an extinction coefficient of 7450 M-1 cm-1 using a NanoDrop 2000/2000c Spectrophotometer (ThermoFisher Cat no. ND-2000) and diluted to 5 mg/mL in 150 mM KCl and 50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... cells were collected by centrifugation and total protein extracts were obtained using M-PER™ Mammalian Protein Extraction Reagent (Thermo-Fisher Scientific, CA, USA) supplemented with pierce protease kit and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were washed twice with 0.2 M sodium acetate for 10 min and mounted from a PBS solution on positively charged microscope slides (Fisher Scientific Cat # 15-188-48). Once mounted and dried ...
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bioRxiv - Cell Biology 2024Quote: The samples from oysters and cells were extracted using Cell lysis buffer for Western and IP (Beyotime Biotechnology, China) and M-PER™ Mammalian Protein Extraction Reagent containing protease inhibitor (Thermo Fisher Scientific, USA) supplemented with protease and phosphatase inhibitors (Beyotime Biotechnology ...
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bioRxiv - Genetics 2023Quote: ... we increased the SDS concentration to 1 % and added not only Proteinase K (20 mg/µl) but also TCEP (Thermo Scientific 77720, 0.5 M) to a final concentration of 0.01 µM ...
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bioRxiv - Neuroscience 2023Quote: ... Their brain tissue was first embedded in cryomolds (Sakura Finetek, Catalog number 4566) filled with M-1 Embedding Matrix (Thermo Scientific, Catalog number 1310) then quickly fresh-frozen on dry ice ...
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bioRxiv - Cancer Biology 2023Quote: ... the dried pellet was resuspended in 8 M urea and quantified using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, USA). A 2 hour incubation with 10 mM DTT at 56 °C was followed by 40 mM iodoacetamide for 45 minutes in the dark at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... were used to inject 100 µL headspace samples into TRACE 1300/ISQ 7000 GC-MS equipped with a TracePLOT TG-BOND Q+ column (30 m x 0.32 mm x 10 µm, Thermo Scientific, Cat. No. 26005-6030) with an injector temperature of 100°C in splitless mode ...
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bioRxiv - Microbiology 2023Quote: ... The cell lysate was mixed with 1 vol of lysis buffer B supplemented with 2% BSA and 50 µl of Dynabeads M-270 Streptavidin (Thermo Fisher Scientific, cat 65305) and incubated at 4°C for 30 min with rotation ...
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bioRxiv - Genetics 2023Quote: ... we increased the SDS concentration to 1 % and added not only Proteinase K (20 mg/µl) but also TCEP (Thermo Scientific 77720, 0.5 M) to a final concentration of 0.01 µM ...
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bioRxiv - Physiology 2023Quote: ... Chromatin complexes were precipitated with 100 µl of Dynabeads M-280 (Sheep anti-mouse, # 11202D; sheep anti-rabbit, #11204; Thermo Fisher Scientific, Waltham, MA). After washes and elution ...
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bioRxiv - Microbiology 2024Quote: Protein was isolated from differentiated cultures using M-PERTM mammalian cell protein lysis reagent and Halt® protease and phosphatase inhibitor cocktail (both from Thermo Scientific, Victoria, Australia). Protein samples were quantified using the BCA protein assay method (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... in CD-1 mouse, Sprague−Dawley rat, and human plasma (Bioreclamation, Inc., Hicksville, NY) by equilibrium dialysis using the RED Device (Thermo Fisher Scientific, Rockford, IL). Compounds were added to pooled plasma (n ≥ 3 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by secondary antibodies (Alexa Fluor Goat anti-Rat 488 Invitrogen #A-11006, 1:1000 and Alexa Fluor Goat anti-Rabbit 594 Invitrogen #A-11012, 1:1000). Coverslips were mounted on slides with Fluoromount-G mounting media containing DAPI (Southern Biotech ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were washed in PBS-T and incubated with the corresponding anti-rabbit or anti-mouse Alexa Fluor 568-conjugated and anti-rat Alexa Fluor 488-conjugated IgG secondary antibody (all 1:1000, Thermo Fisher Scientific, Waltham, MA) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... followed by the secondary antibodies (biotinylated goat anti-rat antibody, Jackson 112-066-072, 1/500; goat anti-rabbit Alexa fluor 635, Life technologies A31577, 1/500) and DAPI (4/1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were washed three times in PBS-T and were incubated for 2 hr with the secondary antibodies Alexa Fluor® 488 or 546 Goat Anti-Mouse/Rat/Rabbit IgG (Life Technologies; 1:200) and then with DAPI (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... sections were incubated for 1 hour at room temperature with goat anti-rat Alexa-Fluor 488 conjugate antibody (Molecular Probe, ThermoFisher Scientific Waltham, MA, USA) diluted 1:500 in PBS ...
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bioRxiv - Cell Biology 2020Quote: Cardiomyocytes were isolated from the left ventricle of 1–2-day-old Sprague-Dawley rats following the protocol from an isolation kit (Cellutron Life Technologies, cat#nc-6031). Myocytes were then plated on cover slips coated with laminin (Life tech #23017-015 ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed with PBS-T and then were incubated with Alexa Fluor 488–conjugated donkey anti–rat IgG (1:500; Invitrogen, California, USA; RRID AB_2535794), Alexa Flour 594–conjugated donkey anti–rabbit IgG (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed and incubated with 1 µg/ml Donkey anti-Rat polyclonal Dylight 488-conjugated antibody (Thermo Fisher Scientific Corporation, OR, USA) at room temperature for 45 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and subsequently for 16 hours at 4°C in blocking solution containing primary antibodies (anti-A2AR mouse antibody, sc-32261 Santa Cruz Biotechnology, at 1:100 and anti-CD31 rat monoclonal antibody, ER-MP12 Thermo Scientific, 1:20). For detection of A2AR and CD31 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ileum and brain of the sub-chronic IP injected animals as well as the CIE rats was extracted using Trizol (Thermo Fisher, Waltham, MA, USA) and the RNeasy MicroElute RNA extraction kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... coverslips were flipped onto 60 mm dishes containing 15 DIV rat hippocampal glial cells cultured in Neurobasal plus medium (Gibco Thermo Fisher Scientific, #A3582901) supplemented with 2 mM glutamine and 1x B27TM plus Neuronal supplement (Gibco Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... and the secondary antibodies solution (1:75 dilutions of Alexa Fluor 488 goat anti-rat and Alexa Fluor 546 goat anti-mouse [Molecular probes, A11006 & A11030, respectively]), always washing with PBS-T in between ...
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bioRxiv - Microbiology 2021Quote: ... The CD3 (Rat anti-CD3 clone CD3-12, P. Moore, UC-Davis, Davis, CA) and CD20 (Rabbit anti-CD20, Thermo Fisher Scientific, Waltham, MA) primary antibodies were applied for 30 m at 1:25 and 1:400 dilutions ...
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bioRxiv - Neuroscience 2020Quote: Tissue sections from male (n = 3) and female (n = 3) rats were mounted on subbed glass slides (Fisher brand Superfrost Plus, Fisher Scientific, Hampton, NH, USA) and desiccated overnight (~16 h ...
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bioRxiv - Neuroscience 2022Quote: ... from E18 Sprague Dawley rats were thawed and cultured according to the manufacturer’s recommendations in a B-27 Plus Neuronal Culture System (Thermo Fisher Scientific, cat. no. A3653401). The thawing and culturing medium (NB Plus + ...
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bioRxiv - Neuroscience 2019Quote: ... at 4°C overnight, rinsed in PBS (3 times, 5 min each) and amplified by Alexa Fluor-488 donkey anti-rat antibody (Life Technologies, A21208, 1:600). Sections were mounted with Fluoromount containing DRAQ-5 (1:1000).
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bioRxiv - Plant Biology 2021Quote: ... the slides were incubated with secondary goat anti-rabbit or goat anti-rat antibodies conjugated with Alexa Fluor 488 or Alexa 555 (Thermo Fisher, diluted 1:100) in PBS containing 0.01 % acetylated BSA at 37 °C for 2h ...
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bioRxiv - Cell Biology 2019Quote: Py-3Y1-S2 rat fibroblast cells were cultured in Dulbecco’s minimum essential medium (DMEM) containing 5% fetal bovine serum (Gibco, Thermo Fisher Scientific K.K., Tokyo, Japan) in the presence of 5% CO2 at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... rat anti-Vasa (1:100, Developmental Studies Hybridoma Bank, RRID: AB_760351) and rabbit anti-GFP (1:2500, Thermo Fisher, cat# A-11122, RRID: AB_221569). Staining was detected with the following conjugated antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were incubated for two hours at room temperature with goat anti-Rat IgG (H+L) Alexa Fluor 488 secondary antibody (ThermoFisher Scientific, diluted 1:200). Antibodies were diluted in 1×PBS with 5% BSA and incubated in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed by washing and cells stained for 1 hr at RT with Alexa Fluor-488 anti-rat secondary antibody (Thermo Scientific, A11006, 1:1000) for CD44 and Alexa Fluor-594 anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... sc-376764) and anti-PbGAPDH rabbit polyclonal serum (1:100 dilution) or anti-mouse CD3 rat monoclonal antibody (Invitrogen, 14-0032-82; 1;100 dilution) for 16 h at 4°C ...
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bioRxiv - Genetics 2020Quote: ... Later optimization steps were performed using genomic DNA from SD rats extracted from tail tissue using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, Waltham, MA). HS rat DNA was extracted from spleen tissue using the Agencourt DNAdvance Kit (Beckman Coulter Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed twice with PB then incubated with species-specific secondary antibodies diluted in PBS-T for two hours (goat anti-rat conjugated to Alexa 488; ThermoFisher; #A-11006; 1:500 and goat anti-rabbit conjugated to Alexa 647; ThermoFisher; A-21247; 1:500). After two final rinses in PB ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and scanned using the standard Affymetrix procedure (Affymetrix GeneChip® Mouse Genome 430 2.0 Array or Affymetrix GeneChip® Rat Genome 230 2.0 Array). Quality control and low level analysis of raw fluorescence intensities was performed using the affy package (v1.64.0 ...
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bioRxiv - Neuroscience 2023Quote: Sections were washed in PBS three times for 5 min each and incubated in secondary antibodies in PBS (goat or donkey anti-rabbit, anti-mouse, or anti-rat fluorescent antibodies; Invitrogen Alexa Fluor; 1:500) for 1 h at room temperature in the dark ...