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Citations for Thermo Fisher :
5701 - 5750 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... rat or rabbit IgG (Invitrogen Clone A10043 Lot 753765; Invitrogen Clone A11055 Lot 2211210; Invitrogen A21447 Lot 872645; Invitrogen A10043 Lot 753765) at 1:500 in PBS+0.3% triton X-100 overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and rat pAbs horseradish peroxidase-conjugated (HRP-Ab) (#111-035-003, #115-035-003, Jackson Immunoresearch Laboratories; #31470, Invitrogen; 1:5000 dilution).
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked with 1% normal goat serum (NGS) in 1X PBS for 1 h followed by incubation with primary antibodies [rat anti-mCherry (Thermo Fisher, #M11217)] overnight at 4°C for standard immunohistochemistry.
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bioRxiv - Immunology 2024Quote: ... Whole- mounts were then washed 3 times for 5 min at room temperature in PBS followed by incubation with Alexa Fluor 568 anti-rat (Invitrogen, 1:500) for 1 hour at room temperature in PBS with 1% BSA and 0.5% Triton-X-100 ...
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bioRxiv - Developmental Biology 2024Quote: ... both yolk sacs and embryos were incubated overnight at 4°C with secondary antibodies to chicken anti-rat Alexa Fluor 488 (Life technologies, A21470) diluted in CAS-Block ...
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bioRxiv - Molecular Biology 2024Quote: ... with 100 nM of either ON-TARGETplus Non-targeting Control siRNA Pool (Dharmacon #D-001810-10-05) or On-TARGETplus Smartpool siRNA targeting rat ACLY protein (Dharmacon #L-089773-02-0005) using lipofectamine RNAimax (Thermofisher Scientific #13778075), as per manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... Excess formalin was quenched with 1 M Glycine and the spheroids were stained with 1 µg/ml Hoechst 33342 solution (62249, ThermoFisher Scientific, Waltham, MA). The organoids were imaged using a 4x objective and appropriate lasers and filters for Hoechst ...
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bioRxiv - Cell Biology 2020Quote: M cell mediated uptake was determined by confocal microscopy of apically exposed 2.0μm fluorescent polystyrene latex beads (Life Technologies; 4×108 final concentration) to human enteroid monolayers expressing or lacking M cells that were cultured in the presence or absence of 106 pathogenic bacteria overnight ...
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bioRxiv - Microbiology 2020Quote: Lysates of infected HeLa cells were prepared by scraping three wells of a 6-well plate into 300-500 μL volume of ice-cold Mammalian Protein Extraction Reagent (M-PER™) buffer (Thermo Fisher Scientific) supplemented with cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154, Fisher Scientific, Waltham, MA, USA). Following purification of total RNA ...
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bioRxiv - Developmental Biology 2020Quote: ... HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154, Fisher Scientific, Waltham, MA, USA). Following purification of total RNA ...
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bioRxiv - Neuroscience 2021Quote: ... After 3 × 15 min washes in 0.1 M PBS and 0.2% TritonX-100 the secondary antibodies were applied (in 0.1 M PBS, 0.2% TritonX-100 and 3% Normal Goat Serum): Alexa647 goat anti-rabbit (Invitrogen #A-21245, 1:1000 dilution). Slices were incubated in the secondary mix for 2 hr at room temperature ...
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bioRxiv - Genomics 2020Quote: ... were treated for 5 min at 65°C and added to 10 μL reverse transcription master mix containing 200 U/μL M-MLV RT enzyme (Invitrogen, Carlsbad, CA, USA). After incubation at 25°C for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Each oligo was diluted to 100 μM in nuclease-free 10 mM Tris pH 8 (diluted from Ambion 1 M Tris pH 8; Invitrogen; cat. no. AM9856). The rRNA depletion mix was generated by combining equal volumes of each 100 μM oligonucleotide stock.
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bioRxiv - Neuroscience 2019Quote: The PSR1 beads were generated by chemical conjugation of a thiolated PSR1 peptoid derivative to magnetic beads (Dynabeads™ M-270 Carboxylic Acid, Invitrogen, Carlsbad CA) as described previously and were provided as a 30mg/ml suspension of beads in bead storage buffer (1xPBS with 0.1% Sodium Azide ...
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bioRxiv - Cell Biology 2020Quote: ... SKOV3-Red and SKOV3-M (1 × 106 of each) were resuspended in PBS with 5 μg/mL Hoechst 33342 (Life Technologies, Carlsbad, USA), and incubated for 20 min at 37°C in water-bath ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 2 µg total RNA by reverse transcription using random primers with M-MLV reverse transcriptase (Thermo Fisher Scientific, 28025013). Real-time PCR was performed on QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Their brain tissue was first embedded in cryomolds (Sakura Finetek, Ref 4566) filled with M-1 Embedding Matrix (Thermo Scientific, Cat. No. 1310) then quickly fresh-frozen on dry ice ...
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bioRxiv - Plant Biology 2020Quote: ... the samples were centrifuged before receiving 50 µL of N-Methyl-M (trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (ThermoFisher Scientific, Waltham, MA, USA), briefly vortexed and incubated at 60 °C for 40 min ...
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bioRxiv - Systems Biology 2020Quote: ... 100 mM D-luciferin was prepared in sterile 0.1 M sodium phosphate buffer (PB) and mixed on an Eppendorf ThermoMixer (Thermo Fisher Scientific, Waltham, MA) at 37° C for two minutes @ 1500 RPM ...
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bioRxiv - Plant Biology 2021Quote: ... 5 μm, 100 Å) precolumn and separated on an Acclaim PepMap RSLC 75 μm × 50 cm (C18, 2 μm, 100 Å) column (Thermo Scientific). The flow rate was 220 nL·min−1 with a total analysis time of 90 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Their brain tissue was first embedded in cryomolds (Sakura Finetek, Ref 4566) filled with M-1 Embedding Matrix (Thermo Scientific, Cat. No. 1310) then quickly fresh-frozen on dry ice ...
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bioRxiv - Systems Biology 2021Quote: ... the samples were centrifuged before receiving 50 μL of N-Methyl-M (trimethylsilyl) trifluoroacetamide (MSTFA) + 1 % trimethylchlorosilane (TMCS) (ThermoFisher Scientific, Waltham, MA, USA), briefly vortexed and incubated at 60 °C for 40 min ...
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bioRxiv - Molecular Biology 2020Quote: ... as a control were independently added to 250 μL of cell lysate solution with 20 μL of Dynabeads M-280 (Thermo Fisher Scientific, US). The IP solutions were incubated at 4°C overnight with rotation ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were loaded on a reverse phase EASY-Spray− column (50 cm × 75 m inner diameter) operated using Easy-nLC− 1200 μ (Thermo Fisher Scientific) coupled online to a Q-Exactive HF-X mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... individual blastomeres were washed thoroughly in Research Wash Medium and either subjected to imaging using the hyperspectral microscope or reaggregated in 2% phytohaemagglutinin M (ThermoFisher Scientific, Waltham, Massachusetts) in Research Cleave Medium to generate chimeric blastocysts ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 μg total RNA was then used to synthesize cDNA using a M-MLV Reverse Transcriptase Enzyme System (Life Technologies, Thermo Fisher Scientific) and OligoT primers ...
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bioRxiv - Developmental Biology 2022Quote: ... 500 ng of RNA was reverse transcribed using Oligo (dT) 12-18mer primers and M-MLV Reverse Transcriptase (Thermo Fisher Scientific, Cat# 280250139) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The head was then immediately sliced off behind the brain and with the eyestalks immersed in 0.13 M cacodylate buffered (pH 6.8; Fisher Scientific, Cat#: 50-980-232) 1% paraformaldehyde with 2% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... 500ng of total RNA was then reverse transcribed with random decamers and M-MLV reverse transcriptase (RT) using the Message Sensor RT Kit (Life Technologies, Carlsbad, CA).
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bioRxiv - Neuroscience 2022Quote: ... with 16 μM BSA in the presence of CaCl2 (1.3mM) and fura-2-acetoxymethyl ester (fura 2-AM, 5 μM: Molecular Probes, Eugene, OR, USA), and they were incubated at 37 °C for 25 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins on the beads were eluted with 300 μl DLB containing 2 mM biotin and precipitated by adding 1/10 volume of 8.74 M Trichloroacetic acid solution (TCA, Fisher Scientific, Cat# BP555-500). Precipitated proteins were washed once with 500 μl of 0.874 M TCA and four times with ice-cold acetone ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were washed twice in 1 × PBS and lysed in 8 M urea supplemented with 1 × Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific, USA). Insoluble cellular material was removed by centrifugation at 18,000 ×g for 20 min at 4°C ...
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bioRxiv - Zoology 2021Quote: ... ASW was filtered to 0.22 µm and the pH adjusted to the test value using either concentrated HCl or 10 M NaOH as measured with an Accumet AP125 pH meter (Fisher Scientific; Pittsburgh, PA). After mounting the tentacle on the glass slide and blotting away excess seawater ...
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bioRxiv - Biophysics 2021Quote: ... 1 μM AF568-labeled actin without or with 2 μM C0-C2 in M-ABB was dispensed by a Multidrop Combi Reagent Dispenser (ThermoFisher Scientific, Waltham, MA) into the 1,536-well assay plates containing the compounds ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed with PBS and incubated with 100 μL of 5 M H2-DCFDA in PBS (Molecular Probes, catalogue number D399). H2O2 levels were assayed on a fusion spectrofluorimeter (PackardBell ...
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bioRxiv - Physiology 2020Quote: ... Purified total RNA was reverse transcribed to cDNA using M-MLV Reverse Transcriptase and a Poly T primer according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Biophysics 2021Quote: ... Protein samples (12 μL) were mixed with 3 μL 5 M DTT and 5 μL 4x NuPAGE™ LDS sample buffer (Thermo Fisher Scientific) and incubated (95 °C ...
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Cryo-EM reveals disrupted human p97 allosteric activation by disease mutations and inhibitor bindingbioRxiv - Biochemistry 2021Quote: ... Digital micrographs of frozen hydrated protein particles were typically recorded at 190000 x magnification with defocus range between -1 to -2.5 μm with a total dose of 50 e-/Å2 on either a 200 kV Glacios or 300 kV Krios (ThermoFisher Scientific, MA, USA) transmission electron microscope fitted with a K3 (Gatan) ...
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bioRxiv - Genetics 2021Quote: ... The amplified library and the pAAV-stop-MCS-bGH plasmid were digested overnight using SpeI-HF and MluI-HF enzymes and purified using Streptavidin M-270 Dynabeads (Thermo Fisher Scientific, 65305) or gel purification respectively (28704 ...
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bioRxiv - Genomics 2021Quote: ... was added and the samples were incubated on a mechanical rotator at room temperature for 30 minutes to activate M-270 Amine-coated Dynabeads (Thermo Fisher Scientific, 14307D). Excess cross-linkers were removed ...
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bioRxiv - Immunology 2021Quote: ... The messenger RNA (mRNA) was isolated from total RNA by Dynabeads M-270 streptavidin with biotin-oligo (dT)25 (Thermo Fisher Scientific, USA). First- and second-strand cDNA synthesis was performed by Superscript reverse transcriptase IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... with one half fixed in 4% formaldehyde in 0.1 M phosphate-buffered saline (PBS; pH 7.3) for histological analysis and the other half extracted in TRIzol™ (Invitrogen, Waltham, MA, USA) for mRNA expression analysis by RT-qPCR.
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bioRxiv - Microbiology 2021Quote: ... Peptides were separated over a 25- cm EasySpray reversed phase LC column (75 μ m, 100 Å, PepMap C18 particles, Thermo Fisher Scientific). The mobile phases (A ...
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bioRxiv - Cancer Biology 2022Quote: ... according to the manufacturer’s instructions with 10 nM siRNA targeting HIC1, HIC1 siGENOME SMART Pool (M-006532-01; Dharmacon/Horizon Discovery, Cambridge, UK) and HIC1 Pre-designed Silencer (AM16708; Ambion/Thermo Fisher Scientific)] ...
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bioRxiv - Cell Biology 2022Quote: ... mice were deeply anesthetized with ketamine (400 mg/kg)/xylazine (50 mg/kg) by intraperitoneal injection and then perfused with 4% paraformaldehyde in 0.1 M phosphate buffer saline (PBS) (Fisher Scientific, Waltham, MA, USA). Liver ...
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bioRxiv - Immunology 2022Quote: PGT145 and VRC26-family antibodies were captured with Protein A columns (Cytiva, 11003493) and gently eluted with 3 M MgCl2 solution (Thermo Fisher Scientific, 21027). Elutions were then buffer exchanged to HEPES buffer (10 mM HEPES pH 8.0 and 75 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... fluorescent FFE skin cells were collected from the FF using forceps and protein was collected using M-Per™ Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) to be used in immunoblotting or frozen and processed by the University of Illinois Protein Science Laboratory for mass spectrometry (MS)-based analyses.
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bioRxiv - Plant Biology 2022Quote: ... cDNA synthesis was carried out in 20 µL final volume reactions with 200 − 500 ng mRNA using Invitrogen™ M-MLV reverse transcriptase (28025013, Thermo Fisher Scientific). qRT-PCR was carried out in 20 µL reactions using 20 ng cDNA and SYBR® Green JumpStart™ ReadyMix™ (S4438 ...
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bioRxiv - Physiology 2023Quote: ... epiphyseal ends of both tibias and femurs were removed and the bone marrow cells were flushed out with M-199 (Thermo Fisher Scientific, USA) supplemented with 10% FBS (heat-inactivated Qualified FBS ...