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Citations for Thermo Fisher :
5451 - 5500 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... epigenetic reprogramming was performed by exposing the DPSC to 10 μM 5-azacytidine (Acros Scientific, Geel, Belgium) in DMEM/F12 containing 2.5% FBS and 10 ng/mL bFGF (Fisher Scientific, Waltham, MA) for 48 hours ...
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bioRxiv - Immunology 2021Quote: ... Lungs were homogenised with a FastPrep-24 5G instrument using TallPrep Lysing Matrix M tubes (MP, Cat# 116949025) in 2 ml Trizol and RNA was isolated using the RiboPure Kit (Invitrogen, Cat# AM1924), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: Where ε’ is the molar extinction coefficient of the fluorescent dye (68000 M-1 cm-1 for FITC, as supplied by Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... connected to an analytical column (EASY-Spray column, 50 m × 75 μm ID, PepMap RSLC C18, 2 μm, 100 Å, Thermo Scientific) using the autosampler of an Easy nLC 1000 (Thermo Scientific ...
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bioRxiv - Biophysics 2019Quote: ... Purified Cas1-Cas2 complex was buffer-changed with 0.5 M Sodium acetate (pH 5.5) and labelled with EZ-Link™ Biotin-LC-Hydrazide (Thermo Scientific, Cat# 21340) and incubated overnight at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... CO-saturated buffer (950 μM CO) was prepared by sparging 3 mL of anaerobic Buffer E for 15 minutes in a Reacti-Vial (Thermo Fisher Scientific). CO was added to the sample to achieve a final concentration of 425 μM ...
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bioRxiv - Genetics 2019Quote: ... The linearized Cas9 plasmids were transcribed into mRNA using the T7 m MESSAGE Kit (Ambition, USA) and gRNA was synthesized using transcript Aid T7 High Yield Transcription Kit (Thermo Scientific, USA). Zebrafish embryos at the one-cell stage were co-injected with 20pg target gRNA and 300pg Cas9 mRNA ...
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bioRxiv - Bioengineering 2019Quote: ... Microscopy images for tdTomato and zsGreen fluorescence were taken of the 10-μ;m sections using an EVOS FL Auto 2 Imaging System (Invitrogen, Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... and CtaAlcOx (ε280 = 94,225 M-1.cm-1) concentration was determined by UV absorption at 280 nm using a Nanodrop ND-200 spectrophotometer (Thermo Fisher Scientific). Final solutions (3-5.5 mg.mL-1 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed once with ice-cold PBS and protein lysates were extracted using M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) with protease and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Mass spectrometry data was acquired using an Orbitrap Lumos and an ultimate 3000 RSLC nano UHPLC equipped with a 300 mm ID × 5 mm Acclaim PepMap m-Precolumn (Thermo Fisher Scientific) and a 75 mm ID × 50 cm 2.1 mm particle Acclaim PepMap RSLC analytical column was used as described before (47).
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bioRxiv - Systems Biology 2020Quote: ... LC-Orbitrap-MS analysis was performed using an ACQUITY UPLC M-Class liquid chromatography system coupled to a QE plus Orbitrap mass spectrometer (Thermo Fisher Scientific). Chromatographic separation was performed using a ZIC-pHILIC column (1.0×150 mm ...
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bioRxiv - Biochemistry 2020Quote: Fresh mouse liver or cells were rinsed in ice-cold PBS and homogenized in 10 volumes of STE buffer (0.25 M sucrose, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, and 1X HALT protease inhibitor cocktail; Thermo Fisher Scientific #78420B) with 15 strokes in a chilled glass-teflon homogenizer ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.03% Triton X-100 in 0.1 M PBS before overnight incubation with primary antibodies (rabbit anti-cldn5, 1:250, Life Technologies, #34-1600) in blocking solution ...
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bioRxiv - Biophysics 2021Quote: DNA-PAINT imaging was performed in Buffer C (2.5 M NaCl; S7653, Sigma-Aldrich in 5x PBS; 14200-059, Gibco Fisher Scientific) supplemented with 1 mM ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... Phages displaying human Fabs were enriched after three rounds of biopanning on biotinylated recombinant human ACE2 immobilised to streptavidin Dynabeads (Dynal M-280, Invitrogen, cat # 112.06D). After the third round of panning ...
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bioRxiv - Cell Biology 2021Quote: ... Peptide was reconstituted in 0.5 M TEAB and processed according to the manufacturer’s protocol for a TMT (Tandem Mass Tag) kit (ThermoFisher Scientific, Waltham, MA, USA). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... the dimerized GFP nanobody LaG-16–G4S–LaG-2 (green lobster) was covalently linked to magnetic beads (Dynabeads M-270 epoxy Thermo Fisher Scientific) as previously described (45) ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram of total RNA was reverse-transcribed to synthesize the first-strand cDNA with M-MuLV Reverse Transcriptases (Thermo Fisher Scientific) and oligo(dT ...
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bioRxiv - Microbiology 2020Quote: ... connected to an analytical column (EASY-Spray column, 50 m × 75 μm ID, PepMap RSLC C18, 2 μm, 100 Å, Thermo Scientific) using the autosampler of an Easy nLC 1000 (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was incubated for 1 hr at 4°C with 200 µL of pre-washed magnetic Dynabeads M-270 Epoxy (Thermo Fisher Scientific) conjugated to rabbit IgG (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... Mice were anesthetized with 10 mg/ml of ketamine and 250 μg/ml of acepromazine and immunized by intramuscular (i.m.) or intranasal (i.n.) route with the corresponding protein formulation along with 50 μl of Imject™ Alum Adjuvant (ThermoFisher Scientific, USA) or 20 μl of Sigma Adjuvant System® oil (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... An aliquot of lysate was taken and diluted 1:5 in 8 M Urea from which protein concentration was determined by BCA assay (Thermo Fisher Scientific). Aliquots corresponding to 100 μg of protein were subsequently diluted in SDC buffer into a 96-well deep-well plate ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were renatured by dialyzing out urea in a step wise manner from 6 M in steps of two at room temperature using Slide–A-Lyzer MINI Dialysis units (Thermo Scientific, USA) with a 10,000 Dalton MWCO ...
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bioRxiv - Cell Biology 2020Quote: ... cloned into the ELD-KKR backbone plasmid (Flemr M. et al., 2013) and transcribed into mRNAs using the mMESSAGE mMACHINE T7 kit (Thermo Fisher Scientific). TALEN mRNAs were polyadenylated with the Poly(A ...
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bioRxiv - Cancer Biology 2021Quote: Cells were washed two times in cold PBS and harvested on ice with M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, #78501), plus freshly added Turbonuclease (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... This population was then treated with TMP (10 μM) and sorted 24 h later for YFP-positive cells with fluorescence into black clear-bottomed 96 well plates (Nunc, Roskilde, Denmark), with a single cell per well ...
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bioRxiv - Molecular Biology 2022Quote: The ubiquitylated worm CMG was then incubated for 30 minutes at 4°C with 2 µl (per 10 µl ubiquitylation reaction mix) magnetic beads (Dynabeads M-270 Epoxy; Life Technologies, 14302D) that had been coupled to anti-worm SLD-5 antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... The extracts were sent to the Department of Chemistry Mass Spectrometry core facility at Texas A&M University for gas chromatography-mass spectrometry (GC-MS) on a DSQ II (Thermo Scientific, TX), and liquid chromatography-mass spectrometry (LC-MS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... equipped with AI1310 autosampler and Thermo TG-5MS column (length, 30 m; internal diameter, 0.25 mm; film, 0.25 μm) (Thermo Fisher Scientific, MA, USA). The analysis employed several internal standards as follows ...
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bioRxiv - Plant Biology 2021Quote: ... Six hundred microliters of the resulting supernatant were first incubated with 200 μl of uncharged Dynabeads M-280 Streptavidin (Thermo Fisher Scientific) for 20 min to minimize nonspecific binding before an incubation with beads loaded with the bait for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... total RNA from HaCaT cells was reverse-transcribed into cDNA using an M-MLV First-Strand Synthesis Kit (Invitrogen cat. C28025-021) with oligo(dT ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μg of RNA from DNAse-treated samples were reverse transcribed using M-MLV for complementary DNA generation (cDNA) (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged donkey anti-rabbit IgG (1:500, ThermoFisher, cat# A-31573) for 2 hr at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... aerobically at 340 nm (6,220 M-1.cm-1) continuously for 1.5 min on a NanoDrop 2000c UV-Vis spectrophotometer (Fisher Scientific, Pittsburgh, PA, USA) using a 10 mm quartz cuvette (Starna Cells ...
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bioRxiv - Biochemistry 2020Quote: ... Survey full-scan MS spectra (from m/z 300–1800) were acquired in the Orbitrap analyzer (Q Exactive, Thermo Fisher Scientific, USA) with resolution r = 70,000 at m/z 400 ...
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bioRxiv - Molecular Biology 2022Quote: ... packed with 1.9 µM C18 ReproSil Pur AQ particles using a Dionex U3000 HPLC coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific). Column temperature was maintained at 60°C with a Sonation column oven ...
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bioRxiv - Microbiology 2022Quote: ... The integrity of the extracted RNA was evaluated in a 1% (m/v) agarose gel and the material was quantified using the nanodrop (ThermoFisher, Massachusetts, USA).
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bioRxiv - Neuroscience 2022Quote: ... Nissl (Neurotrace 640, 1:200 in 0.01 M PBS with 0.1% Triton X-100, 2h, Thermo Fisher Scientific #N21483, Waltham, MA, USA) or DAPI (1:10000 in 0.01 M PBS ...
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bioRxiv - Immunology 2022Quote: ... CD4+ CD25Lo CD127Hi Tconv cells and CD4+ CD25Hi CD127Lo Treg cells were sorted by flow cytometry and stimulated in 96-well cell-culture plates with three times the number of Dynabeads M-450 Tosylactivated beads (Thermo Fisher Scientific) coated with anti-human CD3 mAb (clone HIT3α ...
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bioRxiv - Cell Biology 2022Quote: ... Oocytes were then placed animal hemisphere down in a chamber whose bottom is formed by a fresh ethanol washed microscope cover glass (type-545-M; Thermo Fisher Scientific) and were bathed in Ringer’s solution (110 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... Oocytes were then placed animal hemisphere down in a chamber whose bottom is formed by a fresh ethanol washed microscope cover glass (type-545-M; Thermo Fisher Scientific) and were bathed in Ringer’s solution (110 mM NaCl ...
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bioRxiv - Systems Biology 2019Quote: ... 75 μm × 200 mm (Waters) in a nanoACQUITY UPLC system (Waters, USA) coupled to an LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific). The LTQ-Pribtirap Velos was operated in data-dependent mode with full mass scan acquired for mass range of 400-1,400 m/z ...
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bioRxiv - Biochemistry 2019Quote: ... His6 tagged Ubiquitin M-2C was also labelled using the same protocol but with Cy3B maleimide and AlexaFluor 647 C2 maleimide (Thermo Fisher Scientific) separately ...
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bioRxiv - Pathology 2020Quote: ... the final primer concentration was diluted to 0.5 μM including 5 ng of gDNA in a 20 μl final reaction volume with the SYBR Green qPCR Mix (Thermo Fisher Scientific). The used qRT-PCR program was as follows ...
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bioRxiv - Developmental Biology 2019Quote: ... Total protein was extracted from the homogenized whole kidney lysates using the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, 78501) supplemented with protease (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: 8,000 cells were transfected with 4.8 pmoles of ASH2L siRNA (Dharmacon, M-019831-01-0005) or non-targeting siRNA (Dharmacon, D-001210-02-05) using Lipofectamine RNAiMax (Invitrogen, 13778-150) in OPTI-MEM I Reduced serum medium (Gibco ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were transferred to 0.1 M PB and then incubated with tyramide-conjugated Alexa Fluor 647 (Thermo Fisher Scientific Cat# T20951) for 15 min in dark before H2O2 was added (final concentration 0.0015% ...
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bioRxiv - Systems Biology 2019Quote: Cell lysate proteins from each iPSC time point were extracted by commercial RIPA or M-Per tissue lysis buffer (Thermo Fisher Scientific) followed by brief pulses of sonication with typically 6 pulses at 20% amplitude followed by 5 sec cooldown on ice ...
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bioRxiv - Biochemistry 2019Quote: ... The protein concentration was determined after denaturing the proteins with 6 M guanidine hydrochloride by measuring absorbance at 280 nm and 496 nm with a NanoDrop Spectrophotometer (Thermo Fisher Scientific). The concentration was calculated using the following equation: