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Citations for Thermo Fisher :
5801 - 5850 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Fluorescent anti-rat (Alexa 594) and biotinylated anti-rabbit coupled with streptavidin fluorescent conjugated antibody (Alexa 488) (1:1000, both Life Technologies, Carlsbad, USA).
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bioRxiv - Cell Biology 2021Quote: ... the cell surface localized LAMP-1 protein was detected using immunolabeling with rat monoclonal anti-LAMP-1 antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific; RRID:AB_657536). The cells were moved to fresh media (containing chemical treatments ...
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bioRxiv - Developmental Biology 2021Quote: ... the sections were incubated with a secondary antibody conjugated with a biotin or fluorescence label (for immunofluorescence) or biotin for immunohistochemistry (for CD31, PEG10 and CD45, 1:500 goat anti-rat IgG conjugated with Cy3 (Thermo Fisher Scientific, A10522); for Cytokeratin ...
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bioRxiv - Neuroscience 2020Quote: ... A further incubation of sections commenced in a secondary antibody solution containing 1:500 donkey anti-rat ALEXA 594 (Invitrogen, Burlington, ON, Canada) in 0.1 M TBS for 24 hours at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Secondary antibodies include: Alexa Fluor® 647donkey anti-rabbit IgG (A31573) and Alexa Fluor® 488 donkey anti-rat IgG (A21208) purchased from Life Technologies
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bioRxiv - Immunology 2022Quote: ... sections were with the respective fluorochrome-conjugated secondary antibodies (goat anti-mouse/anti-rabbit/anti-rat, streptavidin etc, conjugated to AlexaFluor 488, 594, 633, 647, all purchased from ThermoFisher Scientific; 1:500) for 2 hours at room temperature in 5% normal goat serum and 0.5% Triton X-100 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Organoids were then washed three times with 1x PBS and incubated with Alexa Fluor 647 Goat anti-Rat IgG secondary antibody (Invitrogen, A-21247, RRID:AB_141778) diluted 1:400 in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: The following secondary antibodies were used at a 1:1000 dilution for tissue and cell staining: donkey anti-rat Alexa Fluor 594 (Thermo Scientific A-21209), goat anti-rat Alexa Fluor 647 (Thermo Scientific A-21247) ...
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bioRxiv - Neuroscience 2022Quote: ... were stained for hematoxylin and eosin or immunostained for glial fibrillary acidic protein (GFAP; rat-anti-GFAP; #13-0300 Thermo Fisher Scientific; RRID:AB_2532994), ionizing calcium binding adaptor protein 1 (IBA1 ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from dissected ipsilateral and contralateral vMB from AAV-TYR- injected rats using mirVana PARIS RNA and Native Protein Purification Kit (Thermo Fisher Scientific # AM1556). RNA concentration was determined using a NanoDrop ND-1000 Spectrophotometer ...
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bioRxiv - Neuroscience 2022Quote: Dried formaldehyde fixed 50mM floating sections of uninjured rat spinal cord were rehydrated in PBS and then treated with 0.25% trypsin EDTA solution (Gibco cat# 25-200-056) or 20mg/mL proteinase K (recombinant PCR grade ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue sections were washed three times with 0.01M PBS and incubated with goat anti-rat Alexa Fluor 488 (1:1000; Invitrogen, cat no. A-11006) and donkey anti-rabbit Alexa Fluor 594 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...
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bioRxiv - Neuroscience 2024Quote: ... Biotin-GCO AGC TOG ATO TTG OGC OC (O = C-phosphorthioate) directed against a unique region of the rat mRNA was synthesized by Invitrogen (Waltham, MA, USA). The corresponding GenBank accession number and ODN position within the mRNA sequence are NM_017171.2 and 420-439 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked using 2.5% fish gelatin in a solution of 0.05% Tween20 in PBS followed by incubation with primary anti-Ki67 (ThermoFisher, rat, 14-5698-82), incubated over-night at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells and rat neurons were plated on 18 mm coverslips that had been coated with poly-D-lysine (Thermo Fisher Scientific, ICN10269491) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... culture conditions were established using a mix of 50% Janvier Labs Rat Serum Sprague Dawley RjHan SD (male only) and 50% DMEM FluoroBrite (Thermo Fisher Scientific, A1896701) with incubation at 37°C and a 7% CO2 concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 hr under room temperature followed by an 1-hr incubation of an alexa 488 goat anti-rat secondary antibody (Thermo Fisher, A-11006). The slides were mounted with VECTASHIELD Antifade Mounting Medium with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were 1:30 rat anti-N-cadherin (Developmental Studies Hybridoma Bank, DN-EX #8,) and 1:200 rabbit anti-lucifer yellow (Molecular Probes, A-5750). The secondary antibodies used were 1:500 goat anti-rabbit with Alexa fluor 633 (Molecular Probes ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were labelled for 2 h at room temperature with secondary antibodies (α-rat Alexa fluor 488, α-rabbit Alexa fluor 568, α-rabbit Alexa fluor 594 - Invitrogen) then washed again 3 times in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed in 0.1M PBS and incubated in goat anti-rat Alexa Fluor 568 secondary antibody (1:500, catalog #A11077, Thermo Fisher Scientific; RRID: AB_2534121) for 1 hr ...
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bioRxiv - Microbiology 2023Quote: ... haemoglobin RNA was removed from 1 µg of RNA extracted from blood samples using the GLOBINclear-Mouse/Rat Globin mRNA Removal Kit (Thermo Fisher Scientific; AM1981) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed three times in PBS and incubated with secondary antibody (Alexa-Fluor-647 goat anti-rat, Thermo Fisher, 1:200 dilution) for 1 hour at room temperature ...
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Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: ... wells of 96-well flat bottom microtiter plates were pre-coated with 300 µg/mL collagen I from rat tail (Gibco, Life Technologies, USA) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rat anti-Tbr2 (1:500, Invitrogen 14-4875-80), rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2022Quote: ... brains were fixed for 14 min in freshly prepared 4% paraformaldehyde and incubated overnight in rat anti-CD8 (1:50, Thermo Fisher #MA5-17594) and mouse nc82 (1:40 ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... pCAFs and pNFs were cultured on dishes coated with collagen I from rat tail (12 μg/ml, Gibco, Thermo Fisher Scientific, Waltham, MA). Unless otherwise stated ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... pCAFs and pNFs were cultured on dishes coated with collagen I from rat tail (12 μg/ml, Gibco, Thermo Fisher Scientific, Waltham, MA). Unless otherwise stated ...
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bioRxiv - Cancer Biology 2022Quote: ... The secondary antibodies were used with goat anti-Rat IgM cross-adsorbed secondary antibody DyLight 488 (Thermo fisher, SA5-10010, 1:400 dilution) or goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies used were goat anti-rat Alexa Fluor 488 or goat anti-mouse Alexa Fluor 596 (Thermo Fisher scientific, 1:1000). The incubation with antibodies was accomplished in a humidified chamber for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Brain slices were then incubated either 4 hours at room temperature or overnight at 4°C in 1.5% Normal Donkey Serum in PBS-T with donkey anti-rat DyLight 550 (ThermoFisher, SA5-10029, 1:1000), donkey anti-goat AlexaFluor 488 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2023Quote: ... unconjugated primary antibodies were detected with fluorescently-labelled species appropriate secondary antibodies including Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 and Goat Anti-Rat IgG (H+L) Alexa Fluor 647 (ThermoFisher Scientific, Brisbane, Australia). All IF sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged and washed 2 times in cold flow buffer and resuspended in donkey anti-rat 555 secondary antibody (ThermoFisher, A48270, 1:1000), donkey anti-guinea pig 647 secondary antibody (Biotium ...
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bioRxiv - Neuroscience 2023Quote: All plates required for cell culture were coated with 100µg/mL with collagen rat tail type I (20nM acetic acid/distilled water) overnight before seeded with cells (Sigma-Aldrich; UK, ThermoFisher Scientific; USA). Spinal cords were collected from postnatal 7–14 day old mice and placed in Hanks balance buffer solution (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were stained for 1 h at 37°C with Alexa Fluor 488-labeled chicken anti-rat IgG (Invitrogen, A21470, 1:300) and Alexa Fluor 546-labeled goat anti-mouse IgG (Invitrogen ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... Brain slices were then incubated for 20 h at RT with primary antibodies (chicken anti-GFP, 1:1000, ab13970, Abcam; rat anti-mcherry, 1:1000, Thermo Fisher Scientific, M11217) in PBS-based blocking solution containing 0.1% TX ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice with perm buffer and then incubated with 20 μL of perm buffer containing 10% rat serum (ThermoFisher 24-5555-94) for 20 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then incubated overnight at 4°C with the following primary antibodies diluted in 1% Normal Goat Serum: Rat anti-Ki67 conjugated FITC (1:50, Invitrogen, 11-5698-82), Mouse anti-active/pro-caspase3 (1:20 ...
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bioRxiv - Microbiology 2024Quote: ... cells were probed with Alexa Fluor 568-conjugated goat anti-rat antibody or 488-conjugated goat anti-rabbit antibody (Invitrogen-Molecular Probes, Paisley, U.K.), diluted 1:200 or 1:1000 in PBS ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from whole heart according to the aforementioned procedure and analyzed by means of GeneChip technology (GeneChip Arrays platform with Clariom™ S Assay, Rat; Thermo Fisher Scientific). Standard quality control steps were included to determine total RNA quality with use of Agilent Bioanalyzer 2100 (RNA integrity number (RIN ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then blocked with 1% bovine serum albumin in PBS for 1 hour before incubating with rat anti-GFAP (Thermo Fisher, 13-0300) or rabbit anti-cFos (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μL of each sample were used for liquid-chromatography-mass spectrometry analyses performed on an Acquity M-Class UPLC (Waters Corporation, Milford, MA) and either an Orbitrap Q-Exactive HFX mass spectrometer (Thermo Scientific, San Jose, CA) or an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... The purified cell lysates were then incubated with pre-washed streptavidin-conjugated paramagnetic beads (DYNAL™ Dynabeads™ M-280 Streptavidin; Invitrogen, cat#11205D) for 2 h at 4 °C (preclearing step) ...
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bioRxiv - Physiology 2022Quote: ... 1 μg of total RNA from each sample was reverse transcribed with 40 U of M-MLV Reverse Transcriptase (Thermofisher Scientific, Waltham, MA, USA) using random hexamer primers.
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bioRxiv - Genomics 2020Quote: ... The barcode-UMI-Poly(dT)-flanked cDNAswerecaptured on the streptavidin-coated M-280 dynabeads using Dynabeads™ kilobaseBINDER™ Kit (60101, Invitrogen, Carlsbad, CA), whereas the unbound cDNAs were removed.
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bioRxiv - Physiology 2019Quote: ... in 2 ml of pure nanopure water (16 to18 M∧) before and after stress using a FisherbrandTM TraceableTM conductivity meter (Thermo Scientific, Waltham, MA, USA). The %EL from intact tissues was determined relative to total tissue electrolytes after boiling ...
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bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
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bioRxiv - Developmental Biology 2021Quote: Preparation of lipid/cholesterol depleted fetal bovine serum (FBS): 500mL FBS were stirred overnight at 4°C with 10g Cab-osil M-5 (ACROS Organics 7631-86-9). The mix was then centrifuged for 10min at 3000rpm and the supernatant filtrated under sterile conditions ...
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bioRxiv - Biochemistry 2020Quote: ... with a flow rate of 5 μ l/min and separated on an EASY-Spray HPLC Column (75 μ m × 150 mm, Thermo Fisher Scientific). HPLC gradient was conducted using 5-55% of the mobile phase (75% acetonitrile and 0.1% formic acid ...
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bioRxiv - Biophysics 2021Quote: ... The filtered lysate was diluted 1:2 in purification buffer UA (8 M Urea, 50 mM sodium acetate [Acros Organics, Carlsbad, CA], pH 4). The protein was then purified using a cation exchange HiPrep SP HP 16/10 (Cytiva ...