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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... RNA was isolated from the cells using Trizol Reagent (#15596, TRIzol™ Reagent; Life Technologies, Thermo Fisher Scientific). RNA isolation was succeeded by cDNA preparation from 1µg of total RNA using iScript™ cDNA Synthesis Kit (#1708891 ...
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bioRxiv - Cell Biology 2024Quote: ... hCMEC/D3 cells were plated in the plates or culture dishes at 6 × 104 cells/cm2 and transfected with 10 nM of negative control or human FOXO1 and ETS1 small interfering RNA (siRNA) (Tsingke Biotechnology, Beijing, China) for 12 h using Lipofectamine™ 3000 (Invitrogen, Carlsbad, CA, USA) reagent according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the primers were phosphorylated using T4 Polynucleotide Kinase (#EK0031, Thermo Fisher Scientific™). After the ligation step ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% dialyzed FBS (Gibco A33820-01) was used and 100µM of ethanolamine ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were maintained in either Essential 8 medium (E8) (Cat # A1517001, ThermoFisher Scientific) or mTeSR™-Plus (Cat # 100-0276 ...
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bioRxiv - Cell Biology 2024Quote: ... CAN) supplemented with (10% v/v) Fetal Bovine Serum (FBS) (Cat # 10437028, Fisher Scientific, Ottawa, CAN) and housed at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... Brain serial frontal sections (30 μm) were cut on a freezing microtome (Thermo Scientific Microm HM560), and immediately mounted on slides to keep track of the left and right sides of the brain ...
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bioRxiv - Cell Biology 2024Quote: ... transfections were performed with purified plasmids using lipofectamine 3000 (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... CRISPR-Cas9 gene editing was performed according to STEMCELL Technologies’ Genome Editing of Human Pluripotent Stem Cells Using the ArciTect™ CRISPR-Cas9 System Technical Document # 27084 Version 3.0.1 FEB 2022 with slight modification in conjunction with the Neon™ Electroporation System (Cat # MPK5000, ThermoFisher Scientific).
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated using Invitrogen PureLink RNA Mini Kit (Cat # 12183020, ThermoFisher Scientific) with on-column DNase I treatment (Cat# 12185010 ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was isolated using Trizol reagent (Thermo Fisher #15596018), then purified by sodium acetate precipitation and eluted in 10 mM Tris pH7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were passaged once they reached 70-80% confluency in the flask via Trypsin-EDTA (0.05% Trypsin, Cat # 325-044-EL, Wisent; 0.53 mM EDTA, Cat # AM9260G, ThermoFisher Scientific, Waltham, USA).
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bioRxiv - Cell Biology 2024Quote: ... F-Tractin-EGFP cells were grown on DMEM (Gibco Ref. 11965-092) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2024Quote: ... Soluble protein concentration was determined using the Pierce BCA Protein Assay Kit (Cat # PI23225, ThermoFisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... with on-column DNase I treatment (Cat# 12185010, ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... prepared in Hanks Balanced Salt Solution (HBSS; Cat # 14025092, ThermoFisher Scientific) to detach the cardiomyocytes from the growth surface ...
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bioRxiv - Cell Biology 2024Quote: ... the Alexa Fluor 594-conjugated goat anti-rabbit and anti-mouse IgG secondary antibodies (A-11012 and A-11005, Invitrogen, dilution 1:500) were added ...
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bioRxiv - Cell Biology 2024Quote: ... PowerTrack SYBR green master mix (ThermoFisher Scientific Cat. No. A46012) and a manufacturer-recommended protocol for a 20µl reaction were used ...
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bioRxiv - Cell Biology 2024Quote: ... they were incubated with the secondary antibodies diluted in blocking solution: Alexa Fluor 488-conjugated goat anti-chicken IgY (A-11039, Invitrogen, dilution 1:1000) and Alexa Fluor 594-conjugated goat anti-mouse IgG (A-11020 ...
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bioRxiv - Cell Biology 2024Quote: ... they were grown up to 70-90% confluency and trypsinized for 3 min (Gibco, 15400-054). For injection ...
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bioRxiv - Molecular Biology 2024Quote: ... 1× non-essential amino acids (catalog no. 11140050, Gibco), 1× sodium pyruvate (catalog no ...
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bioRxiv - Molecular Biology 2024Quote: ... 1× penicillin-streptomycin (catalog no. 15140163, Gibco), 1× non-essential amino acids (catalog no ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 μM 2-mercaptoethanol (catalog no. 21985023, Gibco), 1 μM CHIR99021 (catalog no ...
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bioRxiv - Molecular Biology 2024Quote: ... 1× GlutaMAX™ (catalog no. 35050061, Gibco), 1× penicillin-streptomycin (catalog no ...
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bioRxiv - Molecular Biology 2024Quote: ... The slides were mounted with ProLong™ Glass Antifade Mountant (catalog no. P36982, Thermo Fisher). Fluorescence images were taken using a confocal laser scanning microscope (catalog no ...
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bioRxiv - Molecular Biology 2024Quote: ... the chamber was removed and the slides were mounted with ProLong™ Glass Antifade Mountant (catalog no. P36982, Thermo Fisher). Fluorescence images were taken using a confocal laser scanning microscope (catalog no ...
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bioRxiv - Molecular Biology 2024Quote: The cells were digested with 0.25% trypsin-EDTA (catalog no. 25200072, Gibco), at 37°C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and then re-suspended in FluoroBrite™ DMEM (catalog no. A1896701, Gibco). The cell suspension was filtered through a 35 μM cell strainer (catalog no ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA solution was subjected to DNase treatment using TURBO DNA-free™ kit (catalog no. AM1907, Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... BioDynamics Laboratory), and then transferred to nitrocellulose membranes (catalog no. 10600003, Cytiva) using a Power Blotter System (catalog no. PB0012, Thermo Fisher). The membranes were blocked with PBST containing 2% skim milk (catalog no ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were treated with Image-iT™ FX Signal Enhancer (I36933, Invitrogen) for 30 min to reduce non-specific binding of fluorophores ...
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bioRxiv - Cell Biology 2024Quote: ... and cell number/ml was counted in Countess 3 (Invitrogen by Thermofisher Scientific). Cell numbers were corrected for trypan blue addition and volume of resuspension and presented as histograms.
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bioRxiv - Cell Biology 2024Quote: ... and Alexa Fluor 594-conjugated goat anti-mouse IgG (A-11020, Invitrogen, dilution 1:1000). After rinsing twice and washing twice the samples with PBST for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... and cell number/ml was counted in Countess 3 (Invitrogen by Thermofisher Scientific). Cell numbers were corrected for trypan blue addition and volume of resuspension and presented as histograms.
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP (A10262, Invitrogen, dilution 1:1000) diluted in blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... Nucleoids stained using 1μL/mL PicoGreen (15 min, 37°C; Thermo Fisher Scientific) or transfection with Tfam-GFP ...
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bioRxiv - Cell Biology 2024Quote: ... 10µl of the mix was injected in a countess chamber slide (Invitrogen), and cell number/ml was counted in Countess 3 (Invitrogen by Thermofisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-KIDINS220 (1:1000, Thermo Fisher, PA5-22116), anti-RTN4b (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The system was delivered by lipofection into macrophages using Lipofectamine CRISPRMAX (Invitrogen). A clonal population was generated and targeted Sanger sequencing at the Egr1 locus was performed (Genewiz ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from cells using TRIzol (Invitrogen) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and assayed on ViiA 7 system (Applied Biosystems) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The HRP signal was assayed using SuperSignal West Pico PLUS substrate (Thermo Scientific). GAPDH was detected using mouse anti-GAPDH (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... with 5% Horse serμ (Invitrogen), 1% Penicillin/Streptomycin ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% FBS (Fisher Scientific) and 1% Penicillin/ Streptomycin (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... MCF10a cells (ATCC CRL-10317) were cultured in DMEM/F12 media (Thermo Fisher Scientific) with 5% Horse serμ (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1% Penicillin/ Streptomycin (Invitrogen). MCF10a cells (ATCC CRL-10317 ...
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bioRxiv - Molecular Biology 2024Quote: ... Hap-1 cells (Horizon C631) were cultured in IMDM (Thermo Fisher Scientific) supplemented with 10% FBS (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The pellet was resuspended in 1× LDS sample buffer (Thermo Fisher) with 0.1 M DTT ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was stopped by the addition of LDS sample buffer (Thermo Fisher). The samples were run on an SDS-PAGE gel ...
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bioRxiv - Cell Biology 2024Quote: RNA of cells was isolated with TRIzol® (Invitrogen, #15596026) reagent according to the manufacturer’s protocol (Life Technologies) ...