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Citations for Thermo Fisher :
5651 - 5700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... were seeded at 20,000 per well in a 96-well plate coated with 75 mL Matrigel (Fisher Scientific) or Cultrex (R & D Systems ...
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bioRxiv - Molecular Biology 2024Quote: After desalting by molecular weight cut-off dialysis (Pierce Slide-A-Lyzer, Fisher Scientific), the proteins were stored at −80 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were quantified using the Pierce BCA Protein Assay Kit (Fisher Scientific). Protein expression and purity were examined using SDS-PAGE (Supplementary Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription mix was composed of 5 µL Maxima H minus Reverse Transcriptase (Thermo Fisher Scientific EP0753), 20 µL 5X RT buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mg/ml Collagenase IV (17104019, ThermoFisher Scientific, Zurich, Switzerland), 2 mM CaCl2 and 2% BSA in PBS at 37°C for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Harvested cells were washed twice with phosphate-buffered saline (PBS) with 0.04% Bovine albumin Fraction V (Thermo Fisher Scientific 15260037), and resuspended with PBS to achieve single-cell suspensions.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 0,1% (vol/vol) 2-mercaptoethanol (Gibco). For in vivo infections ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... supplemented with 1% (vol/vol) fetal bovine serum (FBS, Gibco), 1% (vol/vol ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 1% (vol/vol) penicillin/streptomycin (Gibco), 0,1% (vol/vol ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Tg tachyzoites were maintained in vitro by serial passage on confluent monolayer of human foreskin fibroblasts (HFF, ATCC SCRC-1041) in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 1% (vol/vol ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... the slices were washed in 0.3% Triton X-100 in PBS and incubated with a secondary antibody bound with Alexa Fluor 647 (Invitrogen, A31571). The sections were washed 3 times in PBS ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... mounted on glass microscope slides (Thermo Fisher Scientific) and coverslipped with Fluoromount-G medium with DAPI for fluorescence (Invitrogen ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and coverslipped with Fluoromount-G medium with DAPI for fluorescence (Invitrogen, 00-4959-52).
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bioRxiv - Molecular Biology 2024Quote: ... DynabeadsTM Silane Viral NA kit (ThermoFisher, 37011D) was used to purify 1st strand cDNA and ATAC fragments according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... calcein-AM (5 µg/mL, ThermoFisher Scientific) was added to the well to stain the cells on the outer surface of the membrane of the transwell ...
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bioRxiv - Neuroscience 2024Quote: ... the culture medium was replaced with Neurobasal™ medium (GIBCO by Life Technologies). This medium was supplemented with B27 ...
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bioRxiv - Neuroscience 2024Quote: ... Mg2+ (Gibco, #14175-053), incubated on ice for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... and goat anti-chicken Alexa Fluor 488 (Thermo Fisher Scientific Inc).
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bioRxiv - Immunology 2024Quote: ... with data acquired on a ViiA Real-Time PCR System (Applied Biosystems). Manual RT-qPCR was carried out using TaqMan primer/probes (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... and then by live cell gating using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit (Invitrogen, Carlsbad, CA, USA). Flow cytometry was performed on a three-laser CytoFLEX (Beckman Coulter ...
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bioRxiv - Immunology 2024Quote: ... and 1% PSG (Gibco, Gaithersburg, MD, USA). Magnetic bead removal and the evaluation of CAR expression on T cells by flow cytometry were performed on day 6 by staining with a goat anti-mouse F(ab’)2 antibody (Invitrogen ...
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bioRxiv - Immunology 2024Quote: THJ-529 cells (2 x 104 cells/chamber) were plated in a 4-well chamber slide (Nunc, Thermo Scientific, Waltham, MA, USA), incubated overnight ...
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bioRxiv - Immunology 2024Quote: ... and 1% PSG (Gibco, Gaithersburg, MD, USA).
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bioRxiv - Immunology 2024Quote: ... Cells were transferred to the HistoScreen Tissue Cassettes (Thermo Scientific, Waltham, MA, USA) and covered with liquid Histogel ...
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bioRxiv - Immunology 2024Quote: ... TaqMan Low-Density Array (TLDA) cards (Applied Biosystems) were custom designed using TaqMan primer/probes (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: THJ-529 cells (2 x 104 cells/chamber) were plated in a 4-well chamber slide (Nunc, Thermo Scientific, Waltham, MA, USA), incubated overnight ...
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bioRxiv - Immunology 2024Quote: ... tissues (with TRIzol, Life Technologies), or joints [by homogenisation in a TissueLyser II using a Precellys hard tissue Lysing Kit (Bertin ...
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bioRxiv - Immunology 2024Quote: ... were analyzed and calculated by flow cytometry as previously described.(50) T cells isolated from normal donors were stimulated using anti-CD3/CD28 Dynabeads (Life Technologies, Oslo, Norway) at a 3:1 beads-to-cell ratio and then transduced 24 hours after stimulation with lentivirus particles at a MOI of 3 ...
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bioRxiv - Immunology 2024Quote: ... Magnetic bead removal and the evaluation of CAR expression on T cells by flow cytometry were performed on day 6 by staining with a goat anti-mouse F(ab’)2 antibody (Invitrogen, Carlsbad, CA, USA). CART cells were harvested and cryopreserved on day 8 for future experiments ...
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bioRxiv - Immunology 2024Quote: ... Cells were then fixed and permeabilized (FIX & PERM Cell Fixation & Cell Permeabilization Kit, Life Technologies, Oslo, Norway) and stained for CD3 (clone UCHT1 ...
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bioRxiv - Immunology 2024Quote: ... Slides were dehydrated through ethanol and xylene and cover-slipped using a xylene-based mounting medium (Fisher Scientific, Waltham, MA, USA). Samples were examined under bright-field illumination at 20x objective ...
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bioRxiv - Immunology 2024Quote: ... with data acquired on a ViiA Real-Time PCR System (Applied Biosystems, Supplementary Table S1), or with KiCqStart SYBR Green Primers with data acquired and melt curves examined on a QuantStudio 3 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... HistoGel (Thermo Scientific, Waltham, MA, USA) was heated in the microwave for 3 seconds at maximal power and converted to a liquid state ...
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bioRxiv - Immunology 2024Quote: ... ice-cold 100% methanol (Fisher Scientific, Waltham, MA, USA) was added for 7 minutes at -20°C ...
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bioRxiv - Immunology 2024Quote: ... to obtain a pure population as previously described.(50) K562 cell lines were cultured in R10 medium made with RPMI 1640 (Gibco, Gaithersburg, MD, USA), 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... or with KiCqStart SYBR Green Primers with data acquired and melt curves examined on a QuantStudio 3 (ThermoFisher Scientific, Supplementary Table S2). Fold change in expression was calculated using the ΔΔCt method.
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bioRxiv - Immunology 2024Quote: ... were custom designed using TaqMan primer/probes (Applied Biosystems), with data acquired on a ViiA Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed with 3% hydrogen peroxide (Fisher Scientific, Waltham, MA, USA) for 10 minutes to block the activity of endogenous peroxidases ...
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bioRxiv - Immunology 2024Quote: ... and 10% neutral buffered formalin (Fisher Scientific, Waltham, MA, USA) was added for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: RNA was reverse transcribed using a High Capacity Reverse Transcription cDNA kit (Applied Biosystems) after extraction from cultured cells (with RNeasy mini spin-columns ...
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bioRxiv - Immunology 2024Quote: ... Manual RT-qPCR was carried out using TaqMan primer/probes (Applied Biosystems) with data acquired on a ViiA Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... and 1% penicillin-streptomycin-glutamine (PSG, Gibco, Gaithersburg, MD, USA). FTC133 and XTC.UC1 cell lines were cultured in D10 medium made with DMEM (Corning Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... 1x B-27 (Gibco; 17504044), 2 mM GlutaMAX and Primocin ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were ionized using a Nanospray Flex ion source (Thermo Fisher Scientific) with 1,800 V spray voltage and MS acquisition was performed in an Orbitrap Elite spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... and labelled with CellTrace Violet (5 μM, 20 min, 37 °C, Invitrogen). Labelled CD4+ T-cells were co-cultured (4 d ...
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bioRxiv - Neuroscience 2024Quote: ... hiPSC colonies were dissociated into single cells using TrypLE and plated onto the pre-coated MEA plates or coverslips in E8 basal medium (Gibco; A1517001) supplemented with RevitaCell™ ...
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bioRxiv - Immunology 2024Quote: Antigen uptake was measured by flow cytometry analysis of BMDMs incubated with Alexa647-labelled ovalbumin (OVA, 0.1 mg/ml, 1 h, 37 °C, Molecular Probes). To assess antigen presentation ...
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bioRxiv - Microbiology 2024Quote: ... The DNA concentration was measured using a Qubit fluorometer and a dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA), and purity was determined by measuring the A260/A280 absorbance ratio with a NanoDrop spectrophotometer (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... containing 1×B27 Supplement (Gibco, #17504-044), 500 µM L-glutamine (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...