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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and 2mM Glutamine (Invitrogen). Small cell lung cancer cell line DMS53 was grown in Waymouth’s MB medium (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor cells were initially labelled with a viability dye (Cell Trace Violet, ThermoFisher, C34557) before being added to a 96-well plate (1 x 104 cells per well) ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 % fetal bovine serum (FBS, ThermoFisher, A5256701) and 10 % dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... The antibody-chromatin complex was then captured with a mix of protein A and G Dynabeads (Invitrogen) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:1000 2-Mercaptoethanol (ThermoScientific, 21985023) and 1:1000 Gentamicin (Gibco, 1570060). After collection ...
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bioRxiv - Cancer Biology 2024Quote: ... and re-suspended in 115 µL flow buffer with 10 µL of counting beads added per well (Invitrogen, C36950, 125 µL total per well). Plates were then analyzed using a high-throughput reader attached to a LSR Fortessa (BD ...
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bioRxiv - Cancer Biology 2024Quote: ... Small cell lung cancer cell line DMS53 was grown in Waymouth’s MB medium (Gibco, Invitrogen), supplemented with 10% FCS and gentamycin ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was retrotranscribed with Reverse Transcriptase Superscript IV (Invitrogen), qPCR was performed with SYBR Green (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... with 10% FBS and supplemented with 1:100 Minimum Essential Medium Non-Essential Amino Acids (MEM NEAA, Gibco, 1114050), 1:100 Sodium Pyruvate (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... Melanoma IGR cell lines (IGR37 and IGR39) were grown in RPMI w/o HEPES (Gibco, Invitrogen) supplemented with 15% FCS and gentamycin ...
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bioRxiv - Cancer Biology 2024Quote: ... PVDF membrane and T-PER tissue protein extraction reagents were obtained from Thermo Scientific (Waltham, MA). All other reagents were from MilliporeSigma.
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was treated with Luminata Forte Western HRP substrate (MilliporeSigma, Burlington, MA) and then imaged with a MyECL imager (Thermo Fisher, Asheville, NC). Quantification analysis of blots was performed with ImageJ software ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR was performed using Select Master Mix (Thermo Fisher, Asheville, NC) in a BioRad IQ5 detection system (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... and 15 μL of 2X Hoescht 33342 (Thermo Scientific, 62249) (1.7 μM final concentration ...
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bioRxiv - Cancer Biology 2024Quote: Whole-cell lysates were prepared in 1x Cell lysis buffer (CST, 9803) supplemented with protease and phosphatase inhibitor cocktail (Thermo Fisher, 78440), and total protein was measured by Pierce BCA Protein Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase was detected using the Varioskan LUX Plate Reader (Thermo Fisher).
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bioRxiv - Cancer Biology 2024Quote: ... cells were transfected with siTools TCF7L2 siRNA pool using the Lipofectamine RNAiMAX reagent (Thermo Fisher Scientific) to the proportions of 2 μL of 20nM siRNA per well ...
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bioRxiv - Cancer Biology 2024Quote: ... Caki-1 was maintained in McCoy’s 5A medium (Life Technologies, Carlsbad, CA, USA) with 10% FBS and 1% penicillin-streptomycin solution ...
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bioRxiv - Cancer Biology 2024Quote: ... Imaging Buffer was phenol red- and serum-free culture medium (DMEM/F12, Gibco Ref 11039-021) containing any relevant drugs (i.e ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin-streptomycin solution (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... and TCF7L2 (MA5-32240, Thermo Fisher) or p-c-Jun antibody (9164 ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse Transfection of rescue plasmid was performed in 6-well plates using the Lipofectamine 3000 reagent (Thermo Fisher Scientific) to the proportions of 5 μL of 2 μg plasmid per well ...
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bioRxiv - Cancer Biology 2024Quote: ... cells obtained from American Type Culture Collection (ATCC) were cultured in Dulbecco modified Eagle medium (DMEM, Thermo Fisher) supplemented with 10% fetal bovine serum (HyClone ...
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bioRxiv - Cancer Biology 2024Quote: ... coupled to the LTQ Orbitrap XL mass spectrometer (Thermo Scientific, USA). Each biological repeat was measured in 3 technical replicates ...
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bioRxiv - Cancer Biology 2024Quote: Frozen samples (n=8) were OCT embedded and sectioned at 10μm on a Cryostar NX70 cryostat (Thermo Fisher Scientific). Capture sections were fixed with methanol ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA (0.2 μg) was amplified in quadruplate using PowerUP SYBR Green Master mix (Thermo Fisher). Quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Cancer Biology 2024Quote: ... 150mM NaCl (Affymetrix), 1mM EDTA (Affymetrix) ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were fixed with 10% formalin-buffered solution (Thermo Fisher) for at least 48 h before being embedded in paraffin ...
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bioRxiv - Cancer Biology 2024Quote: Total mRNA from 3 CT-PAK2EC and 3 KO-PAK2EC average-sized tumors was extracted using TRIZOL reagent (Invitrogen) according to recommended procedures ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting data from all samples were analysed with the Proteome Discoverer (PD) software (version: 2.5, Thermo Scientific, USA). The data was searched against the human proteome (downloaded ...
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bioRxiv - Cancer Biology 2024Quote: ... 20μL Protein G-coated Dynabeads (Life Technologies) were added to the mixture ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher). Microvascular mouse lung ECs (MLECs ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from cells using TRIzol (Invitrogen, USA) and cDNA synthesis was performed using the Superscript II cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were harvested using cell scraper and lysed for 10 minutes using RIPA buffer (20mM Tris-HCl (Affymetrix), pH-7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... and Sytox™ Green (Invitrogen™, S7020) were added 30 minutes before imaging (4x magnification using DAPI and FITC channels ...
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bioRxiv - Cancer Biology 2024Quote: ... Magnetic Dynabeads (Thermo Fisher) were conjugated with anti-mouse CD102 (clone 3C4 ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs were transfected with si-RNAs Lipofectamine 2000 (Thermo Fisher) according to recommended procedures for 48 h to 96 h.
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bioRxiv - Cancer Biology 2024Quote: ... and cDNA synthesis was performed using the Superscript II cDNA Synthesis Kit (Invitrogen, USA). qRT-PCR was performed using the PowerUp SYBR Green Master Mix ...
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bioRxiv - Cancer Biology 2024Quote: ... red blood cells were lysed with RBC lysis buffer (Gibco) 5 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... 1mM EDTA (Affymetrix), 1mM EGTA (HiMedia) ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were finally developed using chemisluminescent substrate (SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Fisher Scientific) in chemiDoc imaging system (Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2024Quote: ... and imaged on an Evos M7000 (ThermoFisher) with brightfield settings ...
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bioRxiv - Cancer Biology 2024Quote: Total mRNA from 3 siCT and 3 siPAK2 independent experiments was extracted using TRIZOL reagent (Invitrogen) according to recommended procedures ...
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bioRxiv - Cancer Biology 2024Quote: ... KaryoMAX Colcemid solution (Gibco, Catalogue No. 15212012) was added to the media to achieve a final concentration of 0.1 µg/mL for 2.5 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed in 4% PFA for 10 min and stained with DAPI (Invitrogen #R37606) as directed ...
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bioRxiv - Cancer Biology 2024Quote: Cells are transfected twice on consecutive days using 50 nM siRNA with RNAiMAX (Invitrogen) following the protocol recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with cell surface markers and secondary Alexa Fluor 647 goat anti-rabbit (1:200, Invitrogen #A21244) as described previously 4 ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was performed using PowerUpTM SybrTM Green Master Mix (#A25741, Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s protocol and utilized a BioRadTM CFX384 thermocycler ...
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bioRxiv - Cancer Biology 2024Quote: ... or Alexa Fluor 594 (Invitrogen #A11012) anti-rabbit secondary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted using Trizol (Life Technologies #15596026) and RNeasy Kit (Qiagen #74104) ...